Rw5G008070

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003764
Physical Location & Seq
Reverse (-)
8604373 .. 8606275
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G008070.1

Sequence Viewer

Length: 981 bp
ATGAGGCCGATTCTGCTGAAAGGACACGAGAGGCCCTTGACTTTCATCAAGTACAACAAGGACGGAGACCTCCTCTTCTCCTGCGCCAAAGACCACAACCCTAACGTCTGGTTCGCCGACAACGGCGAGCGCCTGGGCACCTACGTCGGCCACAACGGCGCCGTTTGGTGCTGCGACGTCTCCAGAGACTCCTCGCGCCTCATCACCGGCAGCGGGGACTCCACCGCCAAGCTCTGGAATGTTCGGACCGGCGAGCAGCTATTCTCCTTCGACTTCAAATGTCCGGCGAGATCCGTCGAGTTCTCCGTCGGCGATCGCCTCGCCGTCATTACCACTGATCCCTTCATGGGAGTTCCTTCTGCGATTCACGTCAAACGCATCCCTAAGGACCACGCCGATCAGACTGGTGAGTCCGTTCTGACACTGAATGGGCACCAAGGAAAGATAAACAGAGCTGTATGGGGGCCTTTGAACAAAACTATCATTAGCGTTGGAGAAGATTTTGTGATTCGTATTTGGGATTCTGAGACTGGGAAATTACTCAAAAAGAACGAGGCGGCGATTGGTCACAAGGAGGAGATAAAATCACTAGTAAAATCTAAGGATGGTTCTCACTTTCTAACTGGTTCCCGAGATAGATCTGCCAAGCTCTGGGATACTAGAACATTAACTCTTATCAAGACCTACGTGACGCAGCCTCCCATCAACGCAGTTGCAATGTCTCCTCTGCTTGATCATGTCGTGCTTGGTGGTGGCCAGGATGCCACTAAAGTCACCACGACTGATCATCGCGCAGGGCATTTTGAAGCCAAATTCTTTGACAAGGTTCTTGAAAAGGAGATTGGAACTGTTAAAGGGCATTTCTCACCTATTAATGCTTTGGACTTCAATCCTGATGGTAACAGTTTCTCAACCGGAGGTGAGGATGGTTATGTACGTATAAATCATTTTGACCAGGAGTACTTCAGAATTAAGGTTTAG

Protein Analysis

326

Amino Acids

36.2

Weight (kDa)

8.25

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 325 1.1e-125 EIF3I
Beta-prop_TEP1_2nd PF25047 5 - 113 1.5e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 6 - 103 8.2e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 6 - 84 4.4e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 6 - 90 5.9e-07 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 6 - 87 4.2e-10 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 6 - 173 2e-13 CDC20/Fizzy WD40 domain
WDR55 PF24796 7 - 90 3.7e-06 WDR55
Beta-prop_EML_2 PF23414 9 - 88 1.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 109 4.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 12 - 117 1.7e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 183 2.6e-11 WDHD1 first WD40 domain
WD40_Gbeta PF25391 14 - 80 7.5e-09 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 80 3e-09 WD domain, G-beta repeat
Beta-prop_EML PF23409 48 - 266 3.4e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 134 - 228 2.1e-10 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 134 - 254 5.3e-14 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 134 - 249 6.6e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 137 - 314 4.2e-22 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 138 - 222 1e-05 TEP-1 second beta-propeller
WD40_Gbeta PF25391 142 - 265 1.5e-10 G protein beta WD-40 repeat protein
WD40 PF00400 189 - 219 4.2e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 201 - 324 3.9e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 279 - 314 1.4e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 3 cut(s) 137, 158, 432
AccB7I CCANNNNNTGG 2 cut(s) 234, 651
AccII CGCG 2 cut(s) 196, 792
AciI CCGC 3 cut(s) 213, 225, 557
AclWI GGATC 2 cut(s) 285, 332
AcoI YGGCCR 2 cut(s) 148, 754
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 1 cut(s) 949
AcyI GRCGYC 2 cut(s) 159, 177
AfaI GTAC 3 cut(s) 53, 936, 962
AfiI CCNNNNNNNGG 4 cut(s) 213, 234, 347, 651
AgsI TTSAA 5 cut(s) 277, 472, 806, 833, 889
AhlI ACTAGT 1 cut(s) 589
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 3 cut(s) 132, 756, 954
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
AluBI AGCT 4 cut(s) 232, 259, 455, 649
AluI AGCT 4 cut(s) 232, 259, 455, 649
Alw26I GTCTC 5 cut(s) 60, 180, 184, 521, 726
AlwI GGATC 2 cut(s) 285, 332
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 5 cut(s) 5, 32, 148, 464, 754
ApeKI GCWGC 4 cut(s) 171, 210, 256, 694
ApoI RAATTY 1 cut(s) 812
AseI ATTAAT 1 cut(s) 873
AsiSI GCGATCGC 1 cut(s) 316
AspLEI GCGC 5 cut(s) 86, 132, 161, 198, 794
AspS9I GGNCC 4 cut(s) 33, 246, 388, 464
AsuHPI GGTGA 5 cut(s) 196, 419, 766, 858, 932
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 2 cut(s) 246, 388
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 2 cut(s) 140, 435
BalI TGGCCA 1 cut(s) 756
BanI GGYRCC 3 cut(s) 137, 158, 432
BauI CACGAG 1 cut(s) 26
BbvI GCAGC 4 cut(s) 158, 222, 268, 706
BccI CCATC 4 cut(s) 599, 710, 890, 920
BceAI ACGGC 4 cut(s) 139, 146, 172, 308
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 3 cut(s) 134, 758, 956
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 2 cut(s) 733, 784
BcoDI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 2 cut(s) 590, 660
BfoI RGCGCY 2 cut(s) 133, 162
BfuI GTATCC 1 cut(s) 649
BglI GCCNNNNNGGC 1 cut(s) 156
BglII AGATCT 1 cut(s) 638
BisI GCNGC 5 cut(s) 172, 211, 257, 558, 695
BlsI GCNGC 5 cut(s) 173, 212, 258, 559, 696
BmcAI AGTACT 1 cut(s) 962
Bme1390I CCNGG 3 cut(s) 134, 758, 956
Bme18I GGWCC 2 cut(s) 246, 388
BmeT110I CYCGRG 1 cut(s) 630
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 33, 246, 388, 464
BmiI GGNNCC 5 cut(s) 139, 160, 434, 465, 628
BmrFI CCNGG 3 cut(s) 134, 758, 956
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 387, 751
BmuI ACTGGG 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 1 cut(s) 166
BsaAI YACGTR 2 cut(s) 688, 938
BsaHI GRCGYC 2 cut(s) 159, 177
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 133, 436
BsaWI WCCGGW 1 cut(s) 914
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 4 cut(s) 213, 234, 347, 651
Bse118I RCCGGY 2 cut(s) 206, 248
Bse1I ACTGG 3 cut(s) 409, 535, 628
Bse21I CCTNAGG 1 cut(s) 384
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 3 cut(s) 134, 758, 956
BseDI CCNNGG 2 cut(s) 133, 436
BseGI GGATG 4 cut(s) 378, 610, 766, 931
BseLI CCNNNNNNNGG 4 cut(s) 213, 234, 347, 651
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 3 cut(s) 409, 535, 628
BseRI GAGGAG 4 cut(s) 62, 181, 590, 714
BseSI GKGCMC 2 cut(s) 140, 435
BseXI GCAGC 4 cut(s) 158, 222, 268, 706
Bsh1236I CGCG 2 cut(s) 196, 792
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 7, 34, 150, 466, 756
BshNI GGYRCC 3 cut(s) 137, 158, 432
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 4 cut(s) 207, 249, 284, 915
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 4 cut(s) 213, 234, 347, 651
BsmAI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 5 cut(s) 7, 34, 150, 466, 756
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 140, 435
Bsp143I GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
BspACI CCGC 3 cut(s) 213, 225, 557
BspANI GGCC 5 cut(s) 7, 34, 150, 466, 756
BspCNI CTCAG 1 cut(s) 517
BspFNI CGCG 2 cut(s) 196, 792
BspLI GGNNCC 5 cut(s) 139, 160, 434, 465, 628
BspPI GGATC 2 cut(s) 285, 332
BspT107I GGYRCC 3 cut(s) 137, 158, 432
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 2 cut(s) 206, 248
BsrI ACTGG 3 cut(s) 409, 535, 628
BssAI RCCGGY 2 cut(s) 206, 248
BssECI CCNNGG 2 cut(s) 133, 436
BssMI GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
BssNI GRCGYC 2 cut(s) 159, 177
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 3 cut(s) 134, 758, 956
Bst4CI ACNGT 2 cut(s) 850, 905
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 159, 177
BstBAI YACGTR 2 cut(s) 688, 938
BstC8I GCNNGC 2 cut(s) 128, 254
BstDEI CTNAG 3 cut(s) 384, 525, 600
BstF5I GGATG 4 cut(s) 378, 610, 766, 931
BstFNI CGCG 2 cut(s) 196, 792
BstH2I RGCGCY 2 cut(s) 133, 162
BstHHI GCGC 5 cut(s) 86, 132, 161, 198, 794
BstKTI GATC 7 cut(s) 293, 316, 340, 400, 641, 736, 787
BstMAI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BstMBI GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 2 cut(s) 13, 156
BstNI CCWGG 3 cut(s) 134, 758, 956
BstSCI CCNGG 3 cut(s) 132, 756, 954
BstSLI GKGCMC 2 cut(s) 140, 435
BstSNI TACGTA 1 cut(s) 938
BstUI CGCG 2 cut(s) 196, 792
BstV1I GCAGC 4 cut(s) 158, 222, 268, 706
BstX2I RGATCY 2 cut(s) 290, 638
BstYI RGATCY 2 cut(s) 290, 638
Bsu36I CCTNAGG 1 cut(s) 384
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 5 cut(s) 7, 34, 150, 466, 756
BtgZI GCGATG 1 cut(s) 773
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 4 cut(s) 378, 610, 766, 931
BtsIMutI CAGTG 2 cut(s) 333, 422
Cac8I GCNNGC 2 cut(s) 128, 254
CfoI GCGC 5 cut(s) 86, 132, 161, 198, 794
Cfr10I RCCGGY 2 cut(s) 206, 248
Cfr13I GGNCC 4 cut(s) 33, 246, 388, 464
CpoI CGGWCCG 1 cut(s) 246
CseI GACGC 1 cut(s) 700
Csp6I GTAC 3 cut(s) 52, 935, 961
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 2 cut(s) 346, 737
CviQI GTAC 3 cut(s) 52, 935, 961
DdeI CTNAG 3 cut(s) 384, 525, 600
DinI GGCGCC 1 cut(s) 160
DpnI GATC 7 cut(s) 292, 315, 339, 399, 640, 735, 786
DpnII GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
DrdI GACNNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 2 cut(s) 148, 754
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco105I TACGTA 1 cut(s) 938
Eco130I CCWWGG 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 246, 388
Eco57I CTGAAG 1 cut(s) 949
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 630
EcoO109I RGGNCCY 2 cut(s) 33, 464
EcoRII CCWGG 3 cut(s) 132, 756, 954
EcoT14I CCWWGG 1 cut(s) 436
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 2 cut(s) 349, 740
FaiI YATR 5 cut(s) 347, 460, 738, 933, 941
FaqI GGGAC 1 cut(s) 230
FatI CATG 2 cut(s) 345, 736
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 2 cut(s) 733, 784
Fnu4HI GCNGC 5 cut(s) 172, 211, 257, 558, 695
FokI GGATG 4 cut(s) 365, 617, 773, 938
Fsp4HI GCNGC 5 cut(s) 172, 211, 257, 558, 695
FspBI CTAG 2 cut(s) 590, 660
GlaI GCGC 5 cut(s) 85, 131, 160, 197, 793
GluI GCNGC 5 cut(s) 172, 211, 257, 558, 695
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 2 cut(s) 133, 162
HaeIII GGCC 5 cut(s) 7, 34, 150, 466, 756
HapII CCGG 4 cut(s) 207, 249, 284, 915
HgaI GACGC 1 cut(s) 700
HhaI GCGC 5 cut(s) 86, 132, 161, 198, 794
Hin1I GRCGYC 2 cut(s) 159, 177
Hin1II CATG 2 cut(s) 349, 740
Hin6I GCGC 5 cut(s) 84, 130, 159, 196, 792
HinP1I GCGC 5 cut(s) 84, 130, 159, 196, 792
HinfI GANTC 7 cut(s) 10, 188, 218, 364, 410, 508, 521
HpaII CCGG 4 cut(s) 207, 249, 284, 915
HphI GGTGA 5 cut(s) 196, 419, 766, 858, 932
Hpy188I TCNGA 5 cut(s) 246, 402, 420, 526, 968
Hpy188III TCNNGA 6 cut(s) 183, 235, 630, 679, 830, 893
Hpy99I CGWCG 4 cut(s) 149, 179, 299, 311
HpyAV CCTTC 3 cut(s) 277, 352, 366
HpyCH4III ACNGT 2 cut(s) 850, 905
HpyCH4IV ACGT 6 cut(s) 105, 144, 177, 369, 687, 937
HpyCH4V TGCA 1 cut(s) 716
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 156
HpyF3I CTNAG 3 cut(s) 384, 525, 600
HpySE526I ACGT 6 cut(s) 105, 144, 177, 369, 687, 937
Hsp92I GRCGYC 2 cut(s) 159, 177
Hsp92II CATG 2 cut(s) 349, 740
HspAI GCGC 5 cut(s) 84, 130, 159, 196, 792
KasI GGCGCC 1 cut(s) 158
Ksp22I TGATCA 2 cut(s) 733, 784
Kzo9I GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
Lsp1109I GCAGC 4 cut(s) 158, 222, 268, 706
LweI GCATC 2 cut(s) 387, 751
MaeI CTAG 2 cut(s) 590, 660
MaeII ACGT 6 cut(s) 105, 144, 177, 369, 687, 937
MaeIII GTNAC 4 cut(s) 566, 688, 772, 899
MalI GATC 7 cut(s) 292, 315, 339, 399, 640, 735, 786
MboI GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
MboII GAAGA 2 cut(s) 67, 509
MflI RGATCY 2 cut(s) 290, 638
MhlI GDGCHC 2 cut(s) 140, 435
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 3 cut(s) 536, 812, 969
MluNI TGGCCA 1 cut(s) 756
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 3 cut(s) 182, 212, 419
MmeI TCCRAC 1 cut(s) 472
Mox20I TGGCCA 1 cut(s) 756
MscI TGGCCA 1 cut(s) 756
MseI TTAA 4 cut(s) 668, 852, 873, 972
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 4 cut(s) 207, 249, 284, 915
MspR9I CCNGG 3 cut(s) 134, 758, 956
MvaI CCWGG 3 cut(s) 134, 758, 956
MvnI CGCG 2 cut(s) 196, 792
MwoI GCNNNNNNNGC 2 cut(s) 13, 156
NarI GGCGCC 1 cut(s) 159
NdeII GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
NlaIII CATG 2 cut(s) 349, 740
NlaIV GGNNCC 5 cut(s) 139, 160, 434, 465, 628
NmuCI GTSAC 3 cut(s) 566, 688, 772
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 4 cut(s) 10, 364, 508, 521
PflMI CCANNNNNTGG 2 cut(s) 234, 651
PkrI GCNGC 5 cut(s) 173, 212, 258, 559, 696
Ple19I CGATCG 1 cut(s) 316
PleI GAGTC 3 cut(s) 182, 212, 418
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 3 cut(s) 182, 212, 418
Ppu21I YACGTR 2 cut(s) 688, 938
PshBI ATTAAT 1 cut(s) 873
Psp6I CCWGG 3 cut(s) 132, 756, 954
PspGI CCWGG 3 cut(s) 132, 756, 954
PspN4I GGNNCC 5 cut(s) 139, 160, 434, 465, 628
PspPI GGNCC 4 cut(s) 33, 246, 388, 464
PsuI RGATCY 2 cut(s) 290, 638
PvuI CGATCG 1 cut(s) 316
RgaI GCGATCGC 1 cut(s) 316
RsaI GTAC 3 cut(s) 53, 936, 962
RsaNI GTAC 3 cut(s) 52, 935, 961
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SaqAI TTAA 4 cut(s) 668, 852, 873, 972
SatI GCNGC 5 cut(s) 172, 211, 257, 558, 695
Sau3AI GATC 7 cut(s) 290, 313, 337, 397, 638, 733, 784
Sau96I GGNCC 4 cut(s) 33, 246, 388, 464
ScaI AGTACT 1 cut(s) 962
SchI GAGTC 3 cut(s) 182, 212, 419
ScrFI CCNGG 3 cut(s) 134, 758, 956
SduI GDGCHC 2 cut(s) 140, 435
SfaAI GCGATCGC 1 cut(s) 316
SfaNI GCATC 2 cut(s) 387, 751
SfoI GGCGCC 1 cut(s) 160
SgfI GCGATCGC 1 cut(s) 316
SinI GGWCC 2 cut(s) 246, 388
SnaBI TACGTA 1 cut(s) 938
SpeI ACTAGT 1 cut(s) 589
Sse9I AATT 3 cut(s) 536, 812, 969
SsiI CCGC 3 cut(s) 213, 225, 557
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 590, 660
StyD4I CCNGG 3 cut(s) 132, 756, 954
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 850, 905
TaiI ACGT 6 cut(s) 108, 147, 180, 372, 690, 940
TaqI TCGA 2 cut(s) 270, 297
TasI AATT 3 cut(s) 536, 812, 969
TatI WGTACW 2 cut(s) 51, 960
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 4 cut(s) 10, 364, 508, 521
Tru1I TTAA 4 cut(s) 668, 852, 873, 972
Tru9I TTAA 4 cut(s) 668, 852, 873, 972
TscAI CASTG 2 cut(s) 340, 429
TseFI GTSAC 3 cut(s) 566, 688, 772
TseI GCWGC 4 cut(s) 171, 210, 256, 694
Tsp45I GTSAC 3 cut(s) 566, 688, 772
TspDTI ATGAA 2 cut(s) 34, 334
TspGWI ACGGA 4 cut(s) 78, 283, 295, 403
TspRI CASTG 2 cut(s) 340, 429
Van91I CCANNNNNTGG 2 cut(s) 234, 651
VpaK11BI GGWCC 2 cut(s) 246, 388
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 1 cut(s) 812
XspI CTAG 2 cut(s) 590, 660
ZraI GACGTC 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.