MD15G1352700.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003764
Physical Location & Seq
Reverse (-)
42163067 .. 42163297
231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1352700.v1.1.491

Sequence Viewer

Length: 231 bp
ATGAAAGGGCACGAGAGGCCCTTAACTTTCCTCAAATACAACAGGGACGACGACCTCCTCTTCTCCTGCGCCAAGGACCACAACCCCACCGTCTGGTTCGCCAACAACGGCGAGCGCCTCGGGACCTACTGTGACCACAACTGCATCGTTTGGTGCTGCGATGTATCAAAAGACTCCGCCCGCTTCATTACCGGCAGCGCTGACCAGACCGCCAAGCTGTGGAACGTTTAG

Protein Analysis

77

Amino Acids

8.74

Weight (kDa)

6.24

Isoelectric Point (pI)

25.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 76 1e-33 EIF3I
Beta-prop_WDR3_1st PF25173 1 - 76 2.8e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1 - 76 2.6e-11 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 3 - 75 1.1e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 7 - 76 1.6e-09 THOC3 beta-propeller domain
WD40_Gbeta PF25391 9 - 75 3e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 11 - 75 3.8e-06 Prp19 WD40 domain
WD40 PF00400 37 - 75 2e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 93, 219
AciI CCGC 3 cut(s) 177, 181, 210
AclI AACGTT 1 cut(s) 225
AfeI AGCGCT 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 93, 219
AluBI AGCT 1 cut(s) 217
AluI AGCT 1 cut(s) 217
Ama87I CYCGRG 1 cut(s) 119
Aor51HI AGCGCT 1 cut(s) 199
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 156, 195
AspLEI GCGC 3 cut(s) 71, 117, 200
AspS9I GGNCC 3 cut(s) 18, 76, 123
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 2 cut(s) 76, 123
BaeGI GKGCMC 1 cut(s) 12
BauI CACGAG 1 cut(s) 11
BbvI GCAGC 2 cut(s) 143, 207
BceAI ACGGC 1 cut(s) 124
BfoI RGCGCY 2 cut(s) 118, 201
BisI GCNGC 2 cut(s) 157, 196
BlsI GCNGC 2 cut(s) 158, 197
Bme18I GGWCC 2 cut(s) 76, 123
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 3 cut(s) 18, 76, 123
BmiI GGNNCC 1 cut(s) 124
BmsI GCATC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 72, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 219
Bse118I RCCGGY 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 72, 118
BseLI CCNNNNNNNGG 2 cut(s) 93, 219
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 12
BseXI GCAGC 2 cut(s) 143, 207
BshFI GGCC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 59, 136
BslI CCNNNNNNNGG 2 cut(s) 93, 219
BsmFI GGGAC 2 cut(s) 59, 136
BsnI GGCC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 12
BspACI CCGC 3 cut(s) 177, 181, 210
BspANI GGCC 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 72, 118
BssSI CACGAG 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 91, 131
Bst6I CTCTTC 1 cut(s) 65
BstC8I GCNNGC 2 cut(s) 113, 181
BstH2I RGCGCY 2 cut(s) 118, 201
BstHHI GCGC 3 cut(s) 71, 117, 200
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 143, 207
BsuRI GGCC 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 113, 181
CfoI GCGC 3 cut(s) 71, 117, 200
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 18, 76, 123
CviJI RGCY 2 cut(s) 19, 217
CviKI_1 RGCY 2 cut(s) 19, 217
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 72
Eco47I GGWCC 2 cut(s) 76, 123
Eco47III AGCGCT 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 119
EcoO109I RGGNCCY 2 cut(s) 18, 123
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaqI GGGAC 2 cut(s) 59, 136
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 157, 196
Fsp4HI GCNGC 2 cut(s) 157, 196
GlaI GCGC 3 cut(s) 70, 116, 199
GluI GCNGC 2 cut(s) 157, 196
HaeII RGCGCY 2 cut(s) 118, 201
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 192
HhaI GCGC 3 cut(s) 71, 117, 200
Hin6I GCGC 3 cut(s) 69, 115, 198
HinP1I GCGC 3 cut(s) 69, 115, 198
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 192
Hpy188III TCNNGA 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 2 cut(s) 91, 131
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpySE526I ACGT 1 cut(s) 225
HspAI GCGC 3 cut(s) 69, 115, 198
LpnPI CCDG 5 cut(s) 28, 79, 79, 205, 218
Lsp1109I GCAGC 2 cut(s) 143, 207
LweI GCATC 1 cut(s) 153
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 131
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 12
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 5 cut(s) 9, 41, 65, 68, 128
MseI TTAA 1 cut(s) 23
MspI CCGG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 16
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 131
PflMI CCANNNNNTGG 2 cut(s) 93, 219
PkrI GCNGC 2 cut(s) 158, 197
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 3 cut(s) 18, 76, 123
PspPPI RGGWCCY 1 cut(s) 123
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 2 cut(s) 157, 196
Sau96I GGNCC 3 cut(s) 18, 76, 123
SchI GAGTC 1 cut(s) 167
SduI GDGCHC 1 cut(s) 12
SetI ASST 4 cut(s) 57, 128, 219, 228
SfaNI GCATC 1 cut(s) 153
SinI GGWCC 2 cut(s) 76, 123
SsiI CCGC 3 cut(s) 177, 181, 210
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 91, 131
TaiI ACGT 1 cut(s) 228
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 2 cut(s) 156, 195
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 2 cut(s) 17, 175
Van91I CCANNNNNTGG 2 cut(s) 93, 219
VpaK11BI GGWCC 2 cut(s) 76, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.