FvH4_4g35950

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003764
Physical Location & Seq
Forward (+)
33075658 .. 33078125
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35950.t1

Sequence Viewer

Length: 990 bp
ATGAGGCCGATTCTGATGAAAGGGCACGAGAGGCCCTTGACCTTCCTCAAGTACAACCGCGACGGCGACCTCCTCTTCTCCTGCGCCAAGGACCACACCCCTAACGTCTGGTTCGCCGACAACGGCGAGCGCCTCGGCACCTACCGCGGCCACAACGGCGCCGTTTGGTGCTGCGACGTCTCACGTGACTCCTCCCGCCTCATCACCGGCAGCGCGGATCAGACCGCCAAGCTCTGGGATGTCCAGTCCGGCCACCAGGTCTTCACCTTCAACTTCGACTCTCCGACGAGGTCGGTTGAGTTCTCCGTCGGCGATAGGCTCGCCGTCATCACTACCGATCCGTTCATGGAGCAGACTTCTGCTATTCACGTCAAACGCATCGCCGCCGATCCTGCAGATCAGACTGCGGAGTCGATTCTGACACTGAAGATTACTCCTCAGGGGAGGATGAAGATCAACAGAGCTGTGTGGGGGCCGTTGAACAGAACTATAATAAGCGCCGGAGAAGATGCTATCATTCGTATCTGGGATTCTGAGACTGGAAAGCTACTTCAAGAGAACCAGAAGGAGGATGGTCACACAAAAACAATTACTTCGCTAGTAAAGTCTCCTGATGGTTCACACTTCCTAACCGGTTCCCTTGATAAATCTGCCAAGCTATGGGACACTAGGACATTGACTCTTATCAAGACATACCGGACTGAGAGTCCAGTCAATGCAGTTGCTATGTCGCCTCTTCTCAATCACGTTGTACTTGGTGGTGGTCAGGATGCAATCAATGTCACCACGACTGATCATCGTGCTGGGAAATTTGAAGCCAAATTCTATGATAAGATTCTTCAAGAAGAGATTGGAGGTGTTAAAGGGCATTTTGGACCTATCAATGCTTTGGCTTTCAACCCTGATGGTAAAAGTTTCTCAAGTGGAGGTGAAGATGGTTACATACGTTTGCATCACTTCGACCATGATTACTTCAACATCAAGATTTAG

Protein Analysis

330

Amino Acids

36.54

Weight (kDa)

6.67

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 328 9.4e-130 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 91 1.3e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 105 1e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 6 - 93 1.5e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 6 - 176 4.7e-12 CDC20/Fizzy WD40 domain
WDR55 PF24796 7 - 91 3.6e-06 WDR55
Beta-prop_WDR3_2nd PF25172 7 - 80 1.1e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 108 8.7e-09 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 201 2.3e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 13 - 120 6.6e-18 THOC3 beta-propeller domain
WD40_Gbeta PF25391 13 - 90 1.6e-07 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 80 1.6e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 47 - 84 2.6e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 48 - 266 6e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz PF24807 150 - 262 6.6e-10 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 150 - 318 6.3e-15 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 150 - 260 5.6e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 150 - 249 6.5e-14 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 151 - 273 6e-18 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 161 - 321 4.4e-10 WDHD1 first WD40 domain
WD40_Gbeta PF25391 163 - 269 5.3e-12 G protein beta WD-40 repeat protein
WDR55 PF24796 163 - 276 2.3e-06 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 191 - 248 4.9e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 191 - 222 2.8e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 199 - 326 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 286 - 317 9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 2 cut(s) 137, 158
AccB7I CCANNNNNTGG 2 cut(s) 234, 660
AccII CGCG 3 cut(s) 60, 147, 215
AciI CCGC 8 cut(s) 58, 145, 147, 196, 215, 225, 384, 407
AclWI GGATC 3 cut(s) 225, 332, 383
AcoI YGGCCR 2 cut(s) 148, 250
AcsI RAATTY 2 cut(s) 809, 821
AcuI CTGAAG 1 cut(s) 446
AcvI CACGTG 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 159, 177
AfaI GTAC 2 cut(s) 53, 753
AfiI CCNNNNNNNGG 3 cut(s) 234, 568, 660
AgeI ACCGGT 1 cut(s) 632
AgsI TTSAA 7 cut(s) 271, 481, 554, 815, 842, 898, 976
AhdI GACNNNNNGTC 1 cut(s) 705
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 4 cut(s) 232, 464, 547, 658
AluI AGCT 4 cut(s) 232, 464, 547, 658
Alw26I GTCTC 3 cut(s) 184, 530, 612
AlwI GGATC 3 cut(s) 225, 332, 383
AoxI GGCC 5 cut(s) 5, 32, 148, 250, 473
ApeKI GCWGC 2 cut(s) 171, 210
ApoI RAATTY 2 cut(s) 809, 821
AsiGI ACCGGT 1 cut(s) 632
AspLEI GCGC 5 cut(s) 86, 132, 161, 215, 500
AspS9I GGNCC 4 cut(s) 33, 91, 473, 875
AsuHPI GGTGA 4 cut(s) 196, 256, 775, 941
AvaII GGWCC 2 cut(s) 91, 875
AxyI CCTNAGG 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 137, 158
BauI CACGAG 1 cut(s) 26
BbrPI CACGTG 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 158, 222
BccI CCATC 4 cut(s) 566, 608, 899, 929
BceAI ACGGC 6 cut(s) 79, 139, 146, 172, 308, 460
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 3 cut(s) 184, 530, 612
BfaI CTAG 2 cut(s) 599, 669
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 3 cut(s) 133, 162, 501
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 4 cut(s) 148, 172, 211, 384
BlsI GCNGC 4 cut(s) 149, 173, 212, 385
Bme1390I CCNGG 1 cut(s) 257
Bme18I GGWCC 2 cut(s) 91, 875
BmeRI GACNNNNNGTC 1 cut(s) 705
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 33, 91, 473, 875
BmiI GGNNCC 4 cut(s) 139, 160, 474, 637
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 4 cut(s) 387, 499, 760, 961
BpiI GAAGAC 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 32, 904
BsaAI YACGTR 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 452
BsaHI GRCGYC 2 cut(s) 159, 177
BsaJI CCNNGG 3 cut(s) 87, 133, 145
BsaWI WCCGGW 2 cut(s) 632, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 568, 660
Bse118I RCCGGY 2 cut(s) 206, 632
Bse1I ACTGG 3 cut(s) 244, 544, 710
Bse21I CCTNAGG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 452
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseGI GGATG 4 cut(s) 244, 453, 577, 775
BseJI GATNNNNATC 1 cut(s) 452
BseLI CCNNNNNNNGG 3 cut(s) 234, 568, 660
BseMII CTCAG 3 cut(s) 452, 525, 693
BseNI ACTGG 3 cut(s) 244, 544, 710
BseRI GAGGAG 3 cut(s) 62, 181, 426
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 2 cut(s) 158, 222
BseYI CCCAGC 1 cut(s) 803
Bsh1236I CGCG 3 cut(s) 60, 147, 215
BshFI GGCC 5 cut(s) 7, 34, 150, 252, 475
BshNI GGYRCC 2 cut(s) 137, 158
BshTI ACCGGT 1 cut(s) 632
BsiSI CCGG 5 cut(s) 207, 249, 501, 633, 697
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 3 cut(s) 234, 568, 660
BsmAI GTCTC 3 cut(s) 184, 530, 612
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 5 cut(s) 7, 34, 150, 252, 475
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 6 cut(s) 217, 337, 388, 397, 453, 793
BspACI CCGC 8 cut(s) 58, 145, 147, 196, 215, 225, 384, 407
BspANI GGCC 5 cut(s) 7, 34, 150, 252, 475
BspCNI CTCAG 3 cut(s) 451, 526, 694
BspFNI CGCG 3 cut(s) 60, 147, 215
BspLI GGNNCC 4 cut(s) 139, 160, 474, 637
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 3 cut(s) 225, 332, 383
BspT107I GGYRCC 2 cut(s) 137, 158
BsrFI RCCGGY 2 cut(s) 206, 632
BsrI ACTGG 3 cut(s) 244, 544, 710
BssAI RCCGGY 2 cut(s) 206, 632
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 6 cut(s) 217, 337, 388, 397, 453, 793
BssNI GRCGYC 2 cut(s) 159, 177
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 257
Bst6I CTCTTC 3 cut(s) 80, 741, 840
BstACI GRCGYC 2 cut(s) 159, 177
BstBAI YACGTR 1 cut(s) 185
BstC8I GCNNGC 2 cut(s) 128, 321
BstDEI CTNAG 3 cut(s) 438, 534, 702
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 4 cut(s) 244, 453, 577, 775
BstFNI CGCG 3 cut(s) 60, 147, 215
BstH2I RGCGCY 3 cut(s) 133, 162, 501
BstHHI GCGC 5 cut(s) 86, 132, 161, 215, 500
BstKTI GATC 6 cut(s) 220, 340, 391, 400, 456, 796
BstMAI GTCTC 3 cut(s) 184, 530, 612
BstMBI GATC 6 cut(s) 217, 337, 388, 397, 453, 793
BstMWI GCNNNNNNNGC 4 cut(s) 31, 144, 156, 392
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 3 cut(s) 60, 147, 215
BstV1I GCAGC 2 cut(s) 158, 222
BstV2I GAAGAC 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 438
BsuRI GGCC 5 cut(s) 7, 34, 150, 252, 475
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 364
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 4 cut(s) 244, 453, 577, 775
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 128, 321
CfoI GCGC 5 cut(s) 86, 132, 161, 215, 500
Cfr10I RCCGGY 2 cut(s) 206, 632
Cfr13I GGNCC 4 cut(s) 33, 91, 473, 875
Cfr42I CCGCGG 1 cut(s) 148
CsiI ACCWGGT 1 cut(s) 255
Csp6I GTAC 2 cut(s) 52, 752
CspAI ACCGGT 1 cut(s) 632
CviAII CATG 2 cut(s) 346, 965
CviQI GTAC 2 cut(s) 52, 752
DdeI CTNAG 3 cut(s) 438, 534, 702
DinI GGCGCC 1 cut(s) 160
DpnI GATC 6 cut(s) 219, 339, 390, 399, 455, 795
DpnII GATC 6 cut(s) 217, 337, 388, 397, 453, 793
DrdI GACNNNNNNGTC 1 cut(s) 409
DriI GACNNNNNGTC 1 cut(s) 705
DseDI GACNNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 2 cut(s) 148, 250
Eam1104I CTCTTC 3 cut(s) 80, 741, 840
Eam1105I GACNNNNNGTC 1 cut(s) 705
EarI CTCTTC 3 cut(s) 80, 741, 840
Eco130I CCWWGG 1 cut(s) 87
Eco47I GGWCC 2 cut(s) 91, 875
Eco57I CTGAAG 1 cut(s) 446
Eco72I CACGTG 1 cut(s) 185
Eco81I CCTNAGG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 87
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 2 cut(s) 349, 968
FaiI YATR 8 cut(s) 347, 491, 661, 694, 728, 828, 944, 966
FaqI GGGAC 1 cut(s) 677
FatI CATG 2 cut(s) 345, 964
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 4 cut(s) 148, 172, 211, 384
FokI GGATG 4 cut(s) 251, 460, 584, 782
Fsp4HI GCNGC 4 cut(s) 148, 172, 211, 384
FspBI CTAG 2 cut(s) 599, 669
GlaI GCGC 5 cut(s) 85, 131, 160, 214, 499
GluI GCNGC 4 cut(s) 148, 172, 211, 384
GsaI CCCAGC 1 cut(s) 807
HaeII RGCGCY 3 cut(s) 133, 162, 501
HaeIII GGCC 5 cut(s) 7, 34, 150, 252, 475
HapII CCGG 5 cut(s) 207, 249, 501, 633, 697
HhaI GCGC 5 cut(s) 86, 132, 161, 215, 500
Hin1I GRCGYC 2 cut(s) 159, 177
Hin1II CATG 2 cut(s) 349, 968
Hin6I GCGC 5 cut(s) 84, 130, 159, 213, 498
HinP1I GCGC 5 cut(s) 84, 130, 159, 213, 498
HinfI GANTC 9 cut(s) 10, 188, 278, 410, 415, 530, 679, 706, 835
HpaII CCGG 5 cut(s) 207, 249, 501, 633, 697
HphI GGTGA 4 cut(s) 196, 256, 775, 941
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 6 cut(s) 15, 222, 285, 402, 420, 535
Hpy188III TCNNGA 6 cut(s) 554, 611, 688, 767, 842, 982
Hpy8I GTNNAC 1 cut(s) 620
Hpy99I CGWCG 4 cut(s) 65, 179, 289, 311
HpyAV CCTTC 3 cut(s) 52, 277, 559
HpyCH4IV ACGT 6 cut(s) 105, 177, 184, 369, 747, 946
HpyCH4V TGCA 4 cut(s) 395, 719, 773, 952
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 144, 156, 392
HpyF3I CTNAG 3 cut(s) 438, 534, 702
HpySE526I ACGT 6 cut(s) 105, 177, 184, 369, 747, 946
Hsp92I GRCGYC 2 cut(s) 159, 177
Hsp92II CATG 2 cut(s) 349, 968
HspAI GCGC 5 cut(s) 84, 130, 159, 213, 498
KasI GGCGCC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 793
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 6 cut(s) 217, 337, 388, 397, 453, 793
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 2 cut(s) 158, 222
LweI GCATC 4 cut(s) 387, 499, 760, 961
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 2 cut(s) 599, 669
MaeII ACGT 6 cut(s) 105, 177, 184, 369, 747, 946
MaeIII GTNAC 4 cut(s) 185, 575, 781, 938
MalI GATC 6 cut(s) 219, 339, 390, 399, 455, 795
MboI GATC 6 cut(s) 217, 337, 388, 397, 453, 793
MboII GAAGA 9 cut(s) 67, 253, 439, 463, 518, 728, 830, 857, 944
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 3 cut(s) 588, 809, 821
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 5 cut(s) 182, 272, 419, 673, 715
MmeI TCCRAC 1 cut(s) 308
MseI TTAA 1 cut(s) 861
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 5 cut(s) 207, 249, 501, 633, 697
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 3 cut(s) 60, 147, 215
MwoI GCNNNNNNNGC 4 cut(s) 31, 144, 156, 392
NarI GGCGCC 1 cut(s) 159
NdeII GATC 6 cut(s) 217, 337, 388, 397, 453, 793
NlaIII CATG 2 cut(s) 349, 968
NlaIV GGNNCC 4 cut(s) 139, 160, 474, 637
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 3 cut(s) 185, 575, 781
PfeI GAWTC 4 cut(s) 10, 415, 530, 835
PflFI GACNNNGTC 1 cut(s) 289
PflMI CCANNNNNTGG 2 cut(s) 234, 660
PinAI ACCGGT 1 cut(s) 632
PkrI GCNGC 4 cut(s) 149, 173, 212, 385
PleI GAGTC 5 cut(s) 182, 272, 418, 673, 714
PluTI GGCGCC 1 cut(s) 162
PmaCI CACGTG 1 cut(s) 185
PmlI CACGTG 1 cut(s) 185
PpsI GAGTC 5 cut(s) 182, 272, 418, 673, 714
Ppu21I YACGTR 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 255
PspCI CACGTG 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 4 cut(s) 139, 160, 474, 637
PspPI GGNCC 4 cut(s) 33, 91, 473, 875
PstI CTGCAG 1 cut(s) 397
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 2 cut(s) 53, 753
RsaNI GTAC 2 cut(s) 52, 752
SacII CCGCGG 1 cut(s) 148
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 4 cut(s) 148, 172, 211, 384
Sau3AI GATC 6 cut(s) 217, 337, 388, 397, 453, 793
Sau96I GGNCC 4 cut(s) 33, 91, 473, 875
SchI GAGTC 5 cut(s) 182, 272, 419, 673, 715
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 1 cut(s) 27
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 4 cut(s) 387, 499, 760, 961
SfcI CTRYAG 1 cut(s) 393
SfoI GGCGCC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 148
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 2 cut(s) 91, 875
SmlI CTYRAG 2 cut(s) 47, 919
SmoI CTYRAG 2 cut(s) 47, 919
Sse9I AATT 3 cut(s) 588, 809, 821
SsiI CCGC 8 cut(s) 58, 145, 147, 196, 215, 225, 384, 407
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 599, 669
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 6 cut(s) 108, 180, 187, 372, 750, 949
TaqI TCGA 3 cut(s) 276, 413, 960
TasI AATT 3 cut(s) 588, 809, 821
TatI WGTACW 2 cut(s) 51, 751
TauI GCSGC 2 cut(s) 150, 386
TfiI GAWTC 4 cut(s) 10, 415, 530, 835
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 3 cut(s) 185, 575, 781
TseI GCWGC 2 cut(s) 171, 210
Tsp45I GTSAC 3 cut(s) 185, 575, 781
TspDTI ATGAA 3 cut(s) 32, 334, 464
TspGWI ACGGA 2 cut(s) 295, 330
TspRI CASTG 1 cut(s) 429
Tth111I GACNNNGTC 1 cut(s) 289
Van91I CCANNNNNTGG 2 cut(s) 234, 660
VpaK11BI GGWCC 2 cut(s) 91, 875
XapI RAATTY 2 cut(s) 809, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XspI CTAG 2 cut(s) 599, 669
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.