Rh4BG436200

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003764
Physical Location & Seq
Forward (+)
57768869 .. 57770993
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG436200.1

Sequence Viewer

Length: 987 bp
ATGAGGCCGATTCTGATGAAAGGGCACGAGAGGCCCTTGACCTTCCTCAAGTACAACCGCGACGGCGACCTACTCTTCTCCTGCGCCAAGGATCACACCCCCAACGTATGGTTCGCCGACAACGGCGAGCGCCTCGGCACCTACCGCGGCCACAACGGCGCCGTTTGGTGCTGCGACGTCTCCAGAGACTCCTCCCGCCTCATCACCGGTAGCGCAGATCAGAGCGCCAAGCTTTGGGATGTTCAGTCCGGCCAGCAGGTCTTCACCTTCAACTTCGACTCTCCGACAAGGTCGGTGGAGTTCTCCGTCGGCGATAAGCTCGCCGTCATCACCACCGACCCGTTCATGGAGCTCACTTCTGCGATTCACGTCAAACGCATCGCCGGAGACCCTGCCGATCAGACTGCCGAATCGATTCTGACACTCAAGGTTCCTCAGGGGAGAATGAAGATAAACAGAGCTGTTTGGGGGCCGTTGAACAGCACTATCATAAGCGCCGGAGAAGATGCTGTCATTCGTATCTGGGATTCTGAGACTGGAAAACTACTTCAAGAGAACCAGAAGGAAGAGGGTCACACGAAAACGATCACTTCACTAGTAAAATCTCCGGATGGTTCACACTTTCTAACCGGTTCCCTTGATAAATCTGCCAAGCTTTGGGATACTAGAACATTGACTCTTATCAAGACATACCGGACTGAGAGTCCAGTCAATGCAGTTGCAATGTCGCCTCTTCTCAATCATGTTGTACTTGGTGGTGGTCAGGATGCAATCAATGTCACCACAACTGATCATCGTGCTGGGAAATTCGAAGCCAAATTCTATGACAAGATTCTTCAAGAAGAGATTGGAGGTGTTAAAGGGCATTTTGGACCTATTAATGCTTTGGCTTTCAACCCTGATGGTAAAAGTTTCTCAAGTGGAGGTGAAGATGGTTACATACGTCTACATCACTTCGACCATGATTACTTCAACATCAAGATTTAG

Protein Analysis

328

Amino Acids

36.28

Weight (kDa)

6.46

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 327 1.4e-129 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 90 1e-15 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 107 9.5e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 6 - 93 8.8e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 6 - 175 8e-12 CDC20/Fizzy WD40 domain
WDR55 PF24796 7 - 91 8.8e-06 WDR55
Beta-prop_WDR3_2nd PF25172 7 - 80 5.1e-10 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 9 - 87 9.8e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 9 - 91 4.1e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 108 1.6e-08 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 200 2.2e-13 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 13 - 127 6.2e-18 THOC3 beta-propeller domain
WD40 PF00400 42 - 80 4.7e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 43 - 85 3e-07 TEP-1 second beta-propeller
Beta-prop_EML PF23409 48 - 269 2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 67 - 199 4.5e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 147 - 259 1.3e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 148 - 317 2.1e-15 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 148 - 260 3.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 149 - 247 5.7e-14 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 149 - 250 5.9e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 150 - 272 6.7e-18 THOC3 beta-propeller domain
WD40_Gbeta PF25391 162 - 268 3.3e-12 G protein beta WD-40 repeat protein
WDR55 PF24796 162 - 273 1.3e-06 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 190 - 246 4.2e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 190 - 221 2.2e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 198 - 325 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 285 - 316 9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 247
AccB1I GGYRCC 2 cut(s) 137, 158
AccB7I CCANNNNNTGG 3 cut(s) 108, 234, 657
AccI GTMKAC 1 cut(s) 946
AccII CGCG 2 cut(s) 60, 147
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 4 cut(s) 58, 145, 147, 196
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 2 cut(s) 148, 250
AcsI RAATTY 2 cut(s) 806, 818
AcyI GRCGYC 2 cut(s) 159, 177
AfaI GTAC 2 cut(s) 53, 750
AfiI CCNNNNNNNGG 4 cut(s) 108, 234, 346, 657
AgeI ACCGGT 2 cut(s) 206, 629
AgsI TTSAA 6 cut(s) 271, 478, 551, 839, 895, 973
AhdI GACNNNNNGTC 1 cut(s) 702
AhlI ACTAGT 1 cut(s) 595
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 5 cut(s) 232, 319, 352, 461, 655
AluI AGCT 5 cut(s) 232, 319, 352, 461, 655
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 4 cut(s) 180, 184, 381, 527
AlwI GGATC 1 cut(s) 99
Aor13HI TCCGGA 1 cut(s) 607
AoxI GGCC 5 cut(s) 5, 32, 148, 250, 470
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 2 cut(s) 806, 818
AseI ATTAAT 1 cut(s) 879
AsiGI ACCGGT 2 cut(s) 206, 629
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 6 cut(s) 86, 132, 161, 215, 227, 497
AspS9I GGNCC 3 cut(s) 33, 470, 872
AsuHPI GGTGA 5 cut(s) 196, 256, 322, 772, 938
AsuII TTCGAA 1 cut(s) 810
AvaII GGWCC 1 cut(s) 872
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 2 cut(s) 137, 158
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 158
BccI CCATC 3 cut(s) 605, 896, 926
BceAI ACGGC 6 cut(s) 79, 139, 146, 172, 308, 457
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciVI GTATCC 1 cut(s) 655
BclI TGATCA 1 cut(s) 790
BcoDI GTCTC 4 cut(s) 180, 184, 381, 527
BcuI ACTAGT 1 cut(s) 595
BfaI CTAG 2 cut(s) 596, 666
BfoI RGCGCY 4 cut(s) 133, 162, 228, 498
BfuAI ACCTGC 1 cut(s) 247
BfuI GTATCC 1 cut(s) 655
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 2 cut(s) 148, 172
BlsI GCNGC 2 cut(s) 149, 173
Bme18I GGWCC 1 cut(s) 872
BmeRI GACNNNNNGTC 1 cut(s) 702
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 33, 470, 872
BmiI GGNNCC 5 cut(s) 139, 160, 432, 471, 634
BmsI GCATC 3 cut(s) 387, 496, 757
BpiI GAAGAC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 166
Bpu14I TTCGAA 1 cut(s) 810
BpuEI CTTGAG 3 cut(s) 32, 410, 901
Bsa29I ATCGAT 1 cut(s) 413
BsaHI GRCGYC 2 cut(s) 159, 177
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 87, 133, 145
BsaWI WCCGGW 4 cut(s) 206, 607, 629, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 234, 346, 657
Bse118I RCCGGY 2 cut(s) 206, 629
Bse1I ACTGG 2 cut(s) 541, 707
Bse21I CCTNAGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 729
BseAI TCCGGA 1 cut(s) 607
BseCI ATCGAT 1 cut(s) 413
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseGI GGATG 3 cut(s) 244, 616, 772
BseLI CCNNNNNNNGG 4 cut(s) 108, 234, 346, 657
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 3 cut(s) 449, 522, 690
BseNI ACTGG 2 cut(s) 541, 707
BseRI GAGGAG 1 cut(s) 181
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 158
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 2 cut(s) 60, 147
BshFI GGCC 5 cut(s) 7, 34, 150, 252, 472
BshNI GGYRCC 2 cut(s) 137, 158
BshTI ACCGGT 2 cut(s) 206, 629
BshVI ATCGAT 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 7 cut(s) 207, 249, 384, 498, 608, 630, 694
BslI CCNNNNNNNGG 4 cut(s) 108, 234, 346, 657
BsmAI GTCTC 4 cut(s) 180, 184, 381, 527
BsmBI CGTCTC 1 cut(s) 184
BsnI GGCC 5 cut(s) 7, 34, 150, 252, 472
Bso31I GGTCTC 1 cut(s) 381
Bsp119I TTCGAA 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 27, 354
Bsp13I TCCGGA 1 cut(s) 607
Bsp143I GATC 5 cut(s) 91, 217, 397, 585, 790
BspACI CCGC 4 cut(s) 58, 145, 147, 196
BspANI GGCC 5 cut(s) 7, 34, 150, 252, 472
BspCNI CTCAG 3 cut(s) 448, 523, 691
BspDI ATCGAT 1 cut(s) 413
BspEI TCCGGA 1 cut(s) 607
BspFNI CGCG 2 cut(s) 60, 147
BspLI GGNNCC 5 cut(s) 139, 160, 432, 471, 634
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 810
BspT107I GGYRCC 2 cut(s) 137, 158
BspTNI GGTCTC 1 cut(s) 381
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 2 cut(s) 206, 629
BsrI ACTGG 2 cut(s) 541, 707
BssAI RCCGGY 2 cut(s) 206, 629
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 5 cut(s) 91, 217, 397, 585, 790
BssNI GRCGYC 2 cut(s) 159, 177
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 26
Bst6I CTCTTC 4 cut(s) 80, 561, 738, 837
BstACI GRCGYC 2 cut(s) 159, 177
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 3 cut(s) 128, 254, 321
BstDEI CTNAG 3 cut(s) 435, 531, 699
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 3 cut(s) 244, 616, 772
BstFNI CGCG 2 cut(s) 60, 147
BstH2I RGCGCY 4 cut(s) 133, 162, 228, 498
BstHHI GCGC 6 cut(s) 86, 132, 161, 215, 227, 497
BstKTI GATC 5 cut(s) 94, 220, 400, 588, 793
BstMAI GTCTC 4 cut(s) 180, 184, 381, 527
BstMBI GATC 5 cut(s) 91, 217, 397, 585, 790
BstMWI GCNNNNNNNGC 3 cut(s) 31, 144, 156
BstSLI GKGCMC 1 cut(s) 27
BstUI CGCG 2 cut(s) 60, 147
BstV1I GCAGC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 253
Bsu15I ATCGAT 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 435
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 5 cut(s) 7, 34, 150, 252, 472
BsuTUI ATCGAT 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 364
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 244, 616, 772
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 128, 254, 321
CfoI GCGC 6 cut(s) 86, 132, 161, 215, 227, 497
Cfr10I RCCGGY 2 cut(s) 206, 629
Cfr13I GGNCC 3 cut(s) 33, 470, 872
Cfr42I CCGCGG 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 413
Csp6I GTAC 2 cut(s) 52, 749
CspAI ACCGGT 2 cut(s) 206, 629
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 3 cut(s) 346, 743, 962
CviQI GTAC 2 cut(s) 52, 749
DdeI CTNAG 3 cut(s) 435, 531, 699
DinI GGCGCC 1 cut(s) 160
DpnI GATC 5 cut(s) 93, 219, 399, 587, 792
DpnII GATC 5 cut(s) 91, 217, 397, 585, 790
DriI GACNNNNNGTC 1 cut(s) 702
EaeI YGGCCR 2 cut(s) 148, 250
Eam1104I CTCTTC 4 cut(s) 80, 561, 738, 837
Eam1105I GACNNNNNGTC 1 cut(s) 702
EarI CTCTTC 4 cut(s) 80, 561, 738, 837
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 872
Eco53kI GAGCTC 1 cut(s) 352
Eco81I CCTNAGG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 354
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 3 cut(s) 349, 746, 965
FaiI YATR 8 cut(s) 109, 347, 491, 691, 744, 825, 941, 963
FatI CATG 3 cut(s) 345, 742, 961
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 790
FblI GTMKAC 1 cut(s) 946
Fnu4HI GCNGC 2 cut(s) 148, 172
FokI GGATG 3 cut(s) 251, 623, 779
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 2 cut(s) 148, 172
FspBI CTAG 2 cut(s) 596, 666
GlaI GCGC 6 cut(s) 85, 131, 160, 214, 226, 496
GluI GCNGC 2 cut(s) 148, 172
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 4 cut(s) 133, 162, 228, 498
HaeIII GGCC 5 cut(s) 7, 34, 150, 252, 472
HapII CCGG 7 cut(s) 207, 249, 384, 498, 608, 630, 694
HhaI GCGC 6 cut(s) 86, 132, 161, 215, 227, 497
Hin1I GRCGYC 2 cut(s) 159, 177
Hin1II CATG 3 cut(s) 349, 746, 965
Hin6I GCGC 6 cut(s) 84, 130, 159, 213, 225, 495
HinP1I GCGC 6 cut(s) 84, 130, 159, 213, 225, 495
HindIII AAGCTT 2 cut(s) 230, 653
HpaII CCGG 7 cut(s) 207, 249, 384, 498, 608, 630, 694
HphI GGTGA 5 cut(s) 196, 256, 322, 772, 938
Hpy166II GTNNAC 2 cut(s) 617, 947
Hpy188I TCNGA 6 cut(s) 15, 222, 285, 402, 420, 532
Hpy188III TCNNGA 7 cut(s) 183, 551, 608, 685, 764, 839, 979
Hpy8I GTNNAC 2 cut(s) 617, 947
Hpy99I CGWCG 3 cut(s) 65, 179, 311
HpyAV CCTTC 3 cut(s) 52, 277, 556
HpyCH4IV ACGT 4 cut(s) 105, 177, 369, 943
HpyCH4V TGCA 3 cut(s) 716, 722, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 144, 156
HpyF3I CTNAG 3 cut(s) 435, 531, 699
HpySE526I ACGT 4 cut(s) 105, 177, 369, 943
Hsp92I GRCGYC 2 cut(s) 159, 177
Hsp92II CATG 3 cut(s) 349, 746, 965
HspAI GCGC 6 cut(s) 84, 130, 159, 213, 225, 495
KasI GGCGCC 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 607
Ksp22I TGATCA 1 cut(s) 790
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 5 cut(s) 91, 217, 397, 585, 790
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 158
LweI GCATC 3 cut(s) 387, 496, 757
MaeI CTAG 2 cut(s) 596, 666
MaeII ACGT 4 cut(s) 105, 177, 369, 943
MaeIII GTNAC 3 cut(s) 572, 778, 935
MalI GATC 5 cut(s) 93, 219, 399, 587, 792
MboI GATC 5 cut(s) 91, 217, 397, 585, 790
MboII GAAGA 9 cut(s) 67, 253, 460, 515, 578, 725, 827, 854, 941
MhlI GDGCHC 2 cut(s) 27, 354
MluCI AATT 2 cut(s) 806, 818
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 4 cut(s) 182, 272, 670, 712
MmeI TCCRAC 1 cut(s) 308
MroI TCCGGA 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 2 cut(s) 858, 879
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 7 cut(s) 207, 249, 384, 498, 608, 630, 694
MvnI CGCG 2 cut(s) 60, 147
MwoI GCNNNNNNNGC 3 cut(s) 31, 144, 156
NarI GGCGCC 1 cut(s) 159
NdeII GATC 5 cut(s) 91, 217, 397, 585, 790
NlaIII CATG 3 cut(s) 349, 746, 965
NlaIV GGNNCC 5 cut(s) 139, 160, 432, 471, 634
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 572, 778
NspV TTCGAA 1 cut(s) 810
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 6 cut(s) 10, 364, 410, 415, 527, 832
PflFI GACNNNGTC 1 cut(s) 289
PflMI CCANNNNNTGG 3 cut(s) 108, 234, 657
PinAI ACCGGT 2 cut(s) 206, 629
PkrI GCNGC 2 cut(s) 149, 173
PleI GAGTC 4 cut(s) 182, 272, 670, 711
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 4 cut(s) 182, 272, 670, 711
PshBI ATTAAT 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 800
PspN4I GGNNCC 5 cut(s) 139, 160, 432, 471, 634
PspPI GGNCC 3 cut(s) 33, 470, 872
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 2 cut(s) 53, 750
RsaNI GTAC 2 cut(s) 52, 749
SacI GAGCTC 1 cut(s) 354
SacII CCGCGG 1 cut(s) 148
SaqAI TTAA 2 cut(s) 858, 879
SatI GCNGC 2 cut(s) 148, 172
Sau3AI GATC 5 cut(s) 91, 217, 397, 585, 790
Sau96I GGNCC 3 cut(s) 33, 470, 872
SchI GAGTC 4 cut(s) 182, 272, 670, 712
SduI GDGCHC 2 cut(s) 27, 354
SfaNI GCATC 3 cut(s) 387, 496, 757
SfoI GGCGCC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 810
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 1 cut(s) 872
SmlI CTYRAG 3 cut(s) 47, 425, 916
SmoI CTYRAG 3 cut(s) 47, 425, 916
SpeI ACTAGT 1 cut(s) 595
Sse9I AATT 2 cut(s) 806, 818
SsiI CCGC 4 cut(s) 58, 145, 147, 196
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 2 cut(s) 596, 666
SstI GAGCTC 1 cut(s) 354
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 4 cut(s) 108, 180, 372, 946
TaqI TCGA 4 cut(s) 276, 413, 810, 957
TasI AATT 2 cut(s) 806, 818
TatI WGTACW 2 cut(s) 51, 748
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 6 cut(s) 10, 364, 410, 415, 527, 832
Tru1I TTAA 2 cut(s) 858, 879
Tru9I TTAA 2 cut(s) 858, 879
TseFI GTSAC 2 cut(s) 572, 778
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 2 cut(s) 572, 778
TspDTI ATGAA 3 cut(s) 32, 334, 461
TspGWI ACGGA 1 cut(s) 295
Tth111I GACNNNGTC 1 cut(s) 289
Van91I CCANNNNNTGG 3 cut(s) 108, 234, 657
VpaK11BI GGWCC 1 cut(s) 872
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 2 cut(s) 806, 818
XmiI GTMKAC 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 2 cut(s) 596, 666
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.