Rroxscaffold_1G00064570

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003764
Physical Location & Seq
Forward (+)
86437245 .. 86440469
3225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064570.1

Sequence Viewer

Length: 1218 bp
ATGAGGCCGATTCTGCTGAAAGGACACGAGAGGCCCTTGACTTTCATCAAGTACAACAAGGACGGAGACCTTCTCTTCTCCTGCGCCAAAGACCACAACCCTAACGTTTGGTTCGCCGACAACGGCGAGCGCCTCGGCACCTACATCGGCCACAACGGGGCCGTTTGGTGCTGCGACGTCTCCAGAGACTCCTCACGCCTCATCACCGGCAGCGGCGACTCCACCGCCAAGCTCTGGAATGTTCGGACCGGCGAGCAGCTATTCTCCTTCGACTTCAAATGTCCGGCGAGATCCGTCGAGTTCCCCGTCGGCGACCGCCTCGCCGTCATTACCACTGATCCCTTCATGGGAGTTCCTTCTGCAATTCACGTCAAACGCATCGCTAAGGACCACGCGGATCAGACTGGTGAGTCCGTTCTGACACTGAATGGGCACCAAGGAAAGATAAACAGAGCTGTATGGGGGCCTTTGAACAAAACTATCATTAGCGTTGGAGAAGATTTTGTGATTCGTATTTGGGATACTGAGACTGGGAAATTACTTAAAAAGAACGAGGCAGCGATTGGTCACAAGGAGGAGATAAAATCACTAGTAAAATCTAAGGATGGTTCTCACTTTCTAACTGGTTCCCGAGATAGATCTGCCAAGCTCTGGGATACTAGAACATTAACTCTTATCAAGACCTACGTGACGCAGCCTCCCGTCAACGCAGTTGCAATGTCTCCTCTGCTTGATCATGTAGTCAATCTCCCATTGGTTTGCTTAATGATGATACTTACTGCAGTCGTGCTTGGTGGTGGCCAGGATGCCACTAAAGTCACCACGACTGATCATCGCGCAGGGCATTTTGAAGCCAAATTCTTTGACAAGGTTCTTGAAAAGGAGATTGGAACTGTTAAAGGGCATTTCTCACCTATTAATGCTTTGGACTTCAATCCTGATGGTAACAGTTTCTCAACCGGAGGTGAGGATGGTTATGTACGTATAAATCATTTTGACCAGGAGTACTTCAGAATCAAGGTTGTGCTTGGTGCTGGTCAGGATGAATCAAATGTCACACACATTAGGAGGAGCGCCTTAGGAACTGCTGGTGACAGTGACAATGGTGGCTTGAAATTTTTTGGTTGGAAGGAAGCACATGTAGGTTTGGAGGCGTTCCTTGGAATGGTGGATGTCAAATTGTACTGCAGTGATTTACTGGAGATTGCGGCTAGGTAG

Protein Analysis

405

Amino Acids

44.6

Weight (kDa)

6.95

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 340 1.3e-118 EIF3I
Beta-prop_TEP1_2nd PF25047 5 - 106 4.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 5 - 85 9.6e-14 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 90 4e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 6 - 87 1.1e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 6 - 90 1.2e-06 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 6 - 173 3.5e-12 CDC20/Fizzy WD40 domain
WDR55 PF24796 7 - 90 8.2e-06 WDR55
Beta-prop_EML_2 PF23414 9 - 88 2.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 11 - 120 3.7e-13 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 183 1.8e-11 WDHD1 first WD40 domain
WD40_Gbeta PF25391 13 - 80 8.4e-09 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 80 4e-09 WD domain, G-beta repeat
WD40_Prp19 PF24814 134 - 230 9.3e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 134 - 232 1.4e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 134 - 243 7e-16 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 136 - 246 2e-08 CDC20/Fizzy WD40 domain
WDR55 PF24796 137 - 226 3.9e-06 WDR55
Beta-prop_THOC3 PF25174 139 - 242 4.1e-14 THOC3 beta-propeller domain
WD40_Gbeta PF25391 142 - 242 5.8e-09 G protein beta WD-40 repeat protein
WD40 PF00400 189 - 219 5.7e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 201 - 373 5.5e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 294 - 329 1.9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 2 cut(s) 137, 432
AccB7I CCANNNNNTGG 2 cut(s) 234, 651
AccII CGCG 2 cut(s) 395, 837
AciI CCGC 5 cut(s) 213, 225, 316, 395, 1208
AclI AACGTT 1 cut(s) 105
AclWI GGATC 3 cut(s) 285, 332, 405
AcoI YGGCCR 2 cut(s) 148, 799
AcsI RAATTY 2 cut(s) 857, 1115
AcuI CTGAAG 1 cut(s) 994
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 4 cut(s) 53, 981, 1007, 1184
AfiI CCNNNNNNNGG 5 cut(s) 157, 234, 347, 651, 1165
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 6 cut(s) 277, 472, 851, 878, 934, 1114
AhlI ACTAGT 1 cut(s) 589
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 801, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 870, 902
AluBI AGCT 4 cut(s) 232, 259, 455, 649
AluI AGCT 4 cut(s) 232, 259, 455, 649
Alw26I GTCTC 5 cut(s) 60, 180, 184, 521, 726
AlwI GGATC 3 cut(s) 285, 332, 405
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 6 cut(s) 5, 32, 148, 159, 464, 799
ApeKI GCWGC 5 cut(s) 171, 210, 256, 557, 694
ApoI RAATTY 2 cut(s) 857, 1115
AseI ATTAAT 1 cut(s) 918
AspLEI GCGC 4 cut(s) 86, 132, 839, 1076
AspS9I GGNCC 5 cut(s) 33, 159, 246, 388, 464
AsuHPI GGTGA 6 cut(s) 196, 419, 811, 903, 977, 1103
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 2 cut(s) 246, 388
AxyI CCTNAGG 1 cut(s) 1078
BaeGI GKGCMC 1 cut(s) 435
BalI TGGCCA 1 cut(s) 801
BanI GGYRCC 2 cut(s) 137, 432
BauI CACGAG 1 cut(s) 26
BbvI GCAGC 5 cut(s) 158, 222, 268, 569, 706
BccI CCATC 3 cut(s) 599, 935, 965
BceAI ACGGC 3 cut(s) 139, 146, 308
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 2 cut(s) 803, 1001
BciVI GTATCC 2 cut(s) 514, 649
BclI TGATCA 2 cut(s) 733, 829
BcoDI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 3 cut(s) 590, 660, 1212
BfmI CTRYAG 2 cut(s) 780, 1186
BfoI RGCGCY 2 cut(s) 133, 1077
BfuI GTATCC 2 cut(s) 514, 649
BglII AGATCT 1 cut(s) 638
BisI GCNGC 7 cut(s) 172, 211, 214, 257, 558, 695, 1209
BlsI GCNGC 7 cut(s) 173, 212, 215, 258, 559, 696, 1210
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 2 cut(s) 803, 1001
Bme18I GGWCC 2 cut(s) 246, 388
BmeT110I CYCGRG 1 cut(s) 630
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 5 cut(s) 33, 159, 246, 388, 464
BmiI GGNNCC 5 cut(s) 139, 160, 434, 465, 628
BmrFI CCNGG 2 cut(s) 803, 1001
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 387, 796
BmuI ACTGGG 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 384
BsaAI YACGTR 2 cut(s) 688, 983
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 133, 436, 1159
BsaWI WCCGGW 1 cut(s) 959
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 234, 347, 651, 1165
Bse118I RCCGGY 2 cut(s) 206, 248
Bse1I ACTGG 4 cut(s) 409, 535, 628, 1203
Bse21I CCTNAGG 1 cut(s) 1078
Bse3DI GCAATG 1 cut(s) 723
BseBI CCWGG 2 cut(s) 803, 1001
BseDI CCNNGG 3 cut(s) 133, 436, 1159
BseGI GGATG 5 cut(s) 610, 811, 976, 1048, 1177
BseLI CCNNNNNNNGG 5 cut(s) 157, 234, 347, 651, 1165
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 4 cut(s) 409, 535, 628, 1203
BseRI GAGGAG 4 cut(s) 181, 590, 714, 1084
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 5 cut(s) 158, 222, 268, 569, 706
Bsh1236I CGCG 2 cut(s) 395, 837
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 6 cut(s) 7, 34, 150, 161, 466, 801
BshNI GGYRCC 2 cut(s) 137, 432
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 4 cut(s) 207, 249, 284, 960
BslI CCNNNNNNNGG 5 cut(s) 157, 234, 347, 651, 1165
BsmAI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BsmBI CGTCTC 1 cut(s) 184
BsnI GGCC 6 cut(s) 7, 34, 150, 161, 466, 801
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 6 cut(s) 290, 337, 397, 638, 733, 829
BspACI CCGC 5 cut(s) 213, 225, 316, 395, 1208
BspANI GGCC 6 cut(s) 7, 34, 150, 161, 466, 801
BspCNI CTCAG 1 cut(s) 517
BspFNI CGCG 2 cut(s) 395, 837
BspLI GGNNCC 5 cut(s) 139, 160, 434, 465, 628
BspMAI CTGCAG 2 cut(s) 784, 1190
BspPI GGATC 3 cut(s) 285, 332, 405
BspT107I GGYRCC 2 cut(s) 137, 432
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 2 cut(s) 206, 248
BsrI ACTGG 4 cut(s) 409, 535, 628, 1203
BssAI RCCGGY 2 cut(s) 206, 248
BssECI CCNNGG 3 cut(s) 133, 436, 1159
BssMI GATC 6 cut(s) 290, 337, 397, 638, 733, 829
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 2 cut(s) 436, 1159
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 803, 1001
Bst4CI ACNGT 3 cut(s) 895, 950, 1097
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 2 cut(s) 688, 983
BstC8I GCNNGC 2 cut(s) 128, 254
BstDEI CTNAG 4 cut(s) 384, 525, 600, 1078
BstF5I GGATG 5 cut(s) 610, 811, 976, 1048, 1177
BstFNI CGCG 2 cut(s) 395, 837
BstH2I RGCGCY 2 cut(s) 133, 1077
BstHHI GCGC 4 cut(s) 86, 132, 839, 1076
BstKTI GATC 6 cut(s) 293, 340, 400, 641, 736, 832
BstMAI GTCTC 5 cut(s) 60, 180, 184, 521, 726
BstMBI GATC 6 cut(s) 290, 337, 397, 638, 733, 829
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNI CCWGG 2 cut(s) 803, 1001
BstNSI RCATGY 1 cut(s) 1142
BstSCI CCNGG 2 cut(s) 801, 999
BstSFI CTRYAG 2 cut(s) 780, 1186
BstSLI GKGCMC 1 cut(s) 435
BstSNI TACGTA 1 cut(s) 983
BstUI CGCG 2 cut(s) 395, 837
BstV1I GCAGC 5 cut(s) 158, 222, 268, 569, 706
BstX2I RGATCY 2 cut(s) 290, 638
BstYI RGATCY 2 cut(s) 290, 638
Bsu36I CCTNAGG 1 cut(s) 1078
BsuI GTATCC 2 cut(s) 514, 649
BsuRI GGCC 6 cut(s) 7, 34, 150, 161, 466, 801
BtgZI GCGATG 2 cut(s) 364, 818
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 5 cut(s) 610, 811, 976, 1048, 1177
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 4 cut(s) 333, 422, 1102, 1195
Cac8I GCNNGC 2 cut(s) 128, 254
CfoI GCGC 4 cut(s) 86, 132, 839, 1076
Cfr10I RCCGGY 2 cut(s) 206, 248
Cfr13I GGNCC 5 cut(s) 33, 159, 246, 388, 464
CpoI CGGWCCG 1 cut(s) 246
CseI GACGC 1 cut(s) 700
Csp6I GTAC 4 cut(s) 52, 980, 1006, 1183
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 3 cut(s) 346, 737, 1139
CviQI GTAC 4 cut(s) 52, 980, 1006, 1183
DdeI CTNAG 4 cut(s) 384, 525, 600, 1078
DpnI GATC 6 cut(s) 292, 339, 399, 640, 735, 831
DpnII GATC 6 cut(s) 290, 337, 397, 638, 733, 829
DrdI GACNNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 409
EaeI YGGCCR 2 cut(s) 148, 799
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco105I TACGTA 1 cut(s) 983
Eco130I CCWWGG 2 cut(s) 436, 1159
Eco31I GGTCTC 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 246, 388
Eco57I CTGAAG 1 cut(s) 994
Eco81I CCTNAGG 1 cut(s) 1078
Eco88I CYCGRG 1 cut(s) 630
EcoO109I RGGNCCY 2 cut(s) 33, 464
EcoRII CCWGG 2 cut(s) 801, 999
EcoT14I CCWWGG 2 cut(s) 436, 1159
ErhI CCWWGG 2 cut(s) 436, 1159
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 3 cut(s) 349, 740, 1142
FaiI YATR 6 cut(s) 347, 460, 738, 978, 986, 1140
FatI CATG 3 cut(s) 345, 736, 1138
FbaI TGATCA 2 cut(s) 733, 829
Fnu4HI GCNGC 7 cut(s) 172, 211, 214, 257, 558, 695, 1209
FokI GGATG 5 cut(s) 617, 818, 983, 1055, 1184
Fsp4HI GCNGC 7 cut(s) 172, 211, 214, 257, 558, 695, 1209
FspBI CTAG 3 cut(s) 590, 660, 1212
GlaI GCGC 4 cut(s) 85, 131, 838, 1075
GluI GCNGC 7 cut(s) 172, 211, 214, 257, 558, 695, 1209
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 2 cut(s) 133, 1077
HaeIII GGCC 6 cut(s) 7, 34, 150, 161, 466, 801
HapII CCGG 4 cut(s) 207, 249, 284, 960
HgaI GACGC 1 cut(s) 700
HhaI GCGC 4 cut(s) 86, 132, 839, 1076
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 349, 740, 1142
Hin6I GCGC 4 cut(s) 84, 130, 837, 1074
HinP1I GCGC 4 cut(s) 84, 130, 837, 1074
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 7 cut(s) 10, 188, 218, 410, 508, 1014, 1046
HpaII CCGG 4 cut(s) 207, 249, 284, 960
HphI GGTGA 6 cut(s) 196, 419, 811, 903, 977, 1103
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 4 cut(s) 246, 402, 420, 1013
Hpy188III TCNNGA 7 cut(s) 183, 235, 630, 679, 875, 938, 1040
Hpy8I GTNNAC 1 cut(s) 706
Hpy99I CGWCG 3 cut(s) 179, 299, 311
HpyAV CCTTC 5 cut(s) 80, 277, 352, 366, 1123
HpyCH4III ACNGT 3 cut(s) 895, 950, 1097
HpyCH4IV ACGT 5 cut(s) 105, 177, 369, 687, 982
HpyCH4V TGCA 4 cut(s) 362, 716, 782, 1188
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 4 cut(s) 384, 525, 600, 1078
HpySE526I ACGT 5 cut(s) 105, 177, 369, 687, 982
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 349, 740, 1142
HspAI GCGC 4 cut(s) 84, 130, 837, 1074
Ksp22I TGATCA 2 cut(s) 733, 829
Kzo9I GATC 6 cut(s) 290, 337, 397, 638, 733, 829
LmnI GCTCC 1 cut(s) 1071
Lsp1109I GCAGC 5 cut(s) 158, 222, 268, 569, 706
LweI GCATC 2 cut(s) 387, 796
MaeI CTAG 3 cut(s) 590, 660, 1212
MaeII ACGT 5 cut(s) 105, 177, 369, 687, 982
MaeIII GTNAC 7 cut(s) 566, 688, 817, 944, 1054, 1091, 1097
MalI GATC 6 cut(s) 292, 339, 399, 640, 735, 831
MboI GATC 6 cut(s) 290, 337, 397, 638, 733, 829
MboII GAAGA 2 cut(s) 67, 509
MflI RGATCY 2 cut(s) 290, 638
MhlI GDGCHC 1 cut(s) 435
MlsI TGGCCA 1 cut(s) 801
MluCI AATT 5 cut(s) 363, 536, 857, 1115, 1178
MluNI TGGCCA 1 cut(s) 801
MlyI GAGTC 3 cut(s) 182, 212, 419
MmeI TCCRAC 2 cut(s) 472, 1106
Mox20I TGGCCA 1 cut(s) 801
MscI TGGCCA 1 cut(s) 801
MseI TTAA 5 cut(s) 543, 668, 764, 897, 918
Msp20I TGGCCA 1 cut(s) 801
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 4 cut(s) 207, 249, 284, 960
MspR9I CCNGG 2 cut(s) 803, 1001
MvaI CCWGG 2 cut(s) 803, 1001
MvnI CGCG 2 cut(s) 395, 837
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 6 cut(s) 290, 337, 397, 638, 733, 829
NlaIII CATG 3 cut(s) 349, 740, 1142
NlaIV GGNNCC 5 cut(s) 139, 160, 434, 465, 628
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 6 cut(s) 566, 688, 817, 1054, 1091, 1097
NspI RCATGY 1 cut(s) 1142
PciI ACATGT 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 4 cut(s) 10, 508, 1014, 1046
PflMI CCANNNNNTGG 2 cut(s) 234, 651
PkrI GCNGC 7 cut(s) 173, 212, 215, 258, 559, 696, 1210
PleI GAGTC 3 cut(s) 182, 212, 418
PpsI GAGTC 3 cut(s) 182, 212, 418
Ppu21I YACGTR 2 cut(s) 688, 983
PscI ACATGT 1 cut(s) 1138
PshBI ATTAAT 1 cut(s) 918
Psp1406I AACGTT 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 801, 999
PspGI CCWGG 2 cut(s) 801, 999
PspN4I GGNNCC 5 cut(s) 139, 160, 434, 465, 628
PspPI GGNCC 5 cut(s) 33, 159, 246, 388, 464
PstI CTGCAG 2 cut(s) 784, 1190
PsuI RGATCY 2 cut(s) 290, 638
RsaI GTAC 4 cut(s) 53, 981, 1007, 1184
RsaNI GTAC 4 cut(s) 52, 980, 1006, 1183
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SaqAI TTAA 5 cut(s) 543, 668, 764, 897, 918
SatI GCNGC 7 cut(s) 172, 211, 214, 257, 558, 695, 1209
Sau3AI GATC 6 cut(s) 290, 337, 397, 638, 733, 829
Sau96I GGNCC 5 cut(s) 33, 159, 246, 388, 464
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 3 cut(s) 182, 212, 419
ScrFI CCNGG 2 cut(s) 803, 1001
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 2 cut(s) 387, 796
SfcI CTRYAG 2 cut(s) 780, 1186
SinI GGWCC 2 cut(s) 246, 388
SnaBI TACGTA 1 cut(s) 983
SpeI ACTAGT 1 cut(s) 589
Sse9I AATT 5 cut(s) 363, 536, 857, 1115, 1178
SsiI CCGC 5 cut(s) 213, 225, 316, 395, 1208
SspMI CTAG 3 cut(s) 590, 660, 1212
StyD4I CCNGG 2 cut(s) 801, 999
StyI CCWWGG 2 cut(s) 436, 1159
TaaI ACNGT 3 cut(s) 895, 950, 1097
TaiI ACGT 5 cut(s) 108, 180, 372, 690, 985
TaqI TCGA 2 cut(s) 270, 297
TasI AATT 5 cut(s) 363, 536, 857, 1115, 1178
TatI WGTACW 3 cut(s) 51, 1005, 1182
TauI GCSGC 2 cut(s) 216, 1211
TfiI GAWTC 4 cut(s) 10, 508, 1014, 1046
Tru1I TTAA 5 cut(s) 543, 668, 764, 897, 918
Tru9I TTAA 5 cut(s) 543, 668, 764, 897, 918
TscAI CASTG 4 cut(s) 340, 429, 1102, 1195
TseFI GTSAC 6 cut(s) 566, 688, 817, 1054, 1091, 1097
TseI GCWGC 5 cut(s) 171, 210, 256, 557, 694
Tsp45I GTSAC 6 cut(s) 566, 688, 817, 1054, 1091, 1097
TspDTI ATGAA 3 cut(s) 34, 334, 1059
TspGWI ACGGA 3 cut(s) 78, 283, 403
TspRI CASTG 4 cut(s) 340, 429, 1102, 1195
Van91I CCANNNNNTGG 2 cut(s) 234, 651
VpaK11BI GGWCC 2 cut(s) 246, 388
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 2 cut(s) 857, 1115
XceI RCATGY 1 cut(s) 1142
XspI CTAG 3 cut(s) 590, 660, 1212
ZraI GACGTC 1 cut(s) 178
ZrmI AGTACT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.