AT3G02370

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002829
Physical Location & Seq
Forward (+)
484256 .. 487839
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02370.2

Sequence Viewer

Length: 486 bp
ATGGCCGAAGTTGAGGTCAAAAGGGGTAAACTTTGGACGACAACTGGAATTATCCGTACCGGAAAAACATATTGCTTCATAGAAGAGGCTCTGTATTTGAGTGAAATTGGAGAGTTGCAGCTCTTGGGTGATGAGGATGACGTAGTGATTTCATTGAAGGACTTGTATGGGGAAATAGCGGAGGGAAAATATGGCTGCTGTTGGGAAAACTACGAGGTTTATAGATACTTAAAGGGTCTTGGTTACATTCTAGGCAGACATGGAGTTCCTTGGACTACAAAATATGCTGTGAATACTACACCAAGTGATGAAGATGAGTCACTTTGTGCTGCAGAATTTTTCCAAGACAGAGACTCTGTTACCAAACTCCTGAGTGATATGCATATCTGTGATGCAAGACCGGTGTTTGATGTGTATTTGCCAAACAGCCAGTTCAAGAAGTCTTCTCCTGGTGAACCGAGCTTTGTCACTTGCTTCTCGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

161

Amino Acids

18.18

Weight (kDa)

4.65

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 1 - 41 4.2e-09 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 179
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 335
AdeI CACNNNGTG 2 cut(s) 305, 326
AfaI GTAC 1 cut(s) 58
AgeI ACCGGT 1 cut(s) 400
AgsI TTSAA 2 cut(s) 157, 436
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 2 cut(s) 121, 462
AluI AGCT 2 cut(s) 121, 462
Alw26I GTCTC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 118, 195, 329
ApoI RAATTY 1 cut(s) 335
ArsI GACNNNNNNTTYG 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 140, 464
AvaI CYCGRG 1 cut(s) 478
BbsI GAAGAC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 130, 182, 316
BciT130I CCWGG 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 3 cut(s) 119, 196, 330
BlsI GCNGC 3 cut(s) 120, 197, 331
Bme1390I CCNGG 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 478
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 269
BsaWI WCCGGW 2 cut(s) 59, 400
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 2 cut(s) 49, 430
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 2 cut(s) 49, 430
BseXI GCAGC 3 cut(s) 130, 182, 316
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 2 cut(s) 60, 401
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 478
BspACI CCGC 1 cut(s) 179
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 2 cut(s) 49, 430
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 450
Bst6I CTCTTC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 142
BstMAI GTCTC 1 cut(s) 345
BstNI CCWGG 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 448
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 130, 182, 316
BstV2I GAAGAC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 400
Csp6I GTAC 1 cut(s) 57
CspAI ACCGGT 1 cut(s) 400
CviAII CATG 1 cut(s) 260
CviJI RGCY 6 cut(s) 5, 89, 121, 195, 429, 462
CviKI_1 RGCY 6 cut(s) 5, 89, 121, 195, 429, 462
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 1 cut(s) 371
DraIII CACNNNGTG 2 cut(s) 305, 326
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 269
EcoT22I ATGCAT 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 1 cut(s) 263
FaiI YATR 9 cut(s) 70, 80, 168, 192, 222, 261, 285, 380, 384
FatI CATG 1 cut(s) 259
Fnu4HI GCNGC 3 cut(s) 119, 196, 330
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 3 cut(s) 119, 196, 330
FspBI CTAG 1 cut(s) 251
GluI GCNGC 3 cut(s) 119, 196, 330
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 60, 401
Hin1II CATG 1 cut(s) 263
HinfI GANTC 2 cut(s) 317, 353
HpaII CCGG 2 cut(s) 60, 401
HphI GGTGA 2 cut(s) 140, 464
Hpy166II GTNNAC 2 cut(s) 29, 455
Hpy188III TCNNGA 2 cut(s) 370, 436
Hpy8I GTNNAC 2 cut(s) 29, 455
HpyAV CCTTC 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 118, 332, 382, 395
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 263
LpnPI CCDG 7 cut(s) 30, 73, 383, 414, 435, 443, 462
Lsp1109I GCAGC 3 cut(s) 130, 182, 316
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 4 cut(s) 242, 318, 358, 466
MboII GAAGA 3 cut(s) 95, 323, 435
MluCI AATT 3 cut(s) 48, 105, 335
MlyI GAGTC 2 cut(s) 326, 347
MnlI CCTC 5 cut(s) 7, 79, 127, 175, 208
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 387
MspI CCGG 2 cut(s) 60, 401
MspR9I CCNGG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 450
NlaIII CATG 1 cut(s) 263
NmuCI GTSAC 2 cut(s) 318, 466
NsiI ATGCAT 1 cut(s) 384
PinAI ACCGGT 1 cut(s) 400
PkrI GCNGC 3 cut(s) 120, 197, 331
PleI GAGTC 2 cut(s) 325, 347
PpsI GAGTC 2 cut(s) 325, 347
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PstI CTGCAG 1 cut(s) 334
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 3 cut(s) 119, 196, 330
SchI GAGTC 2 cut(s) 326, 347
ScrFI CCNGG 1 cut(s) 450
SetI ASST 5 cut(s) 18, 123, 144, 219, 464
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 3 cut(s) 48, 105, 335
SsiI CCGC 1 cut(s) 179
SspMI CTAG 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 448
StyI CCWWGG 1 cut(s) 269
TaiI ACGT 1 cut(s) 144
TasI AATT 3 cut(s) 48, 105, 335
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TseFI GTSAC 2 cut(s) 318, 466
TseI GCWGC 3 cut(s) 118, 195, 329
Tsp45I GTSAC 2 cut(s) 318, 466
TspDTI ATGAA 3 cut(s) 67, 141, 324
TspGWI ACGGA 1 cut(s) 44
XapI RAATTY 1 cut(s) 335
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.