Rh3BG290900

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002829
Physical Location & Seq
Reverse (-)
29124538 .. 29127058
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG290900.1

Sequence Viewer

Length: 744 bp
ATGGAGCCACTGGATTCTTTGGGAGAGCCTGATATCAATGATGTCGAAGAAGAAGAAGAAGAACAACAAGTTCACTATGCTTCTGGGCCCGCATCCAAATTGCAACCCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAAAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTAGTGGCAACCTATATTACTCCATTGAGGAAGTTCTATTTTTGGTTGAAATAGGGGCTTTAGTTCTCTTGGATGAGAGTGATACTGGCCGCCTTTCCTTGGAAGATGTATATGTGAAGATGTCGGGTGGGAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCTGACAGTGATGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTCCCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGGGGATATCCGCCATCCAGAGCAGAGCTTGAAGCCCTCGAGGGACAATGTAGGAGCATTCCTTTGAAGTTTTGTAATGTGGAGCAGGGACGGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

247

Amino Acids

27.58

Weight (kDa)

4.85

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 39 - 91 1.5e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 683
AccI GTMKAC 1 cut(s) 191
AciI CCGC 5 cut(s) 90, 113, 295, 491, 656
AclWI GGATC 1 cut(s) 614
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 5 cut(s) 170, 254, 538, 677, 712
AjnI CCWGG 3 cut(s) 107, 419, 560
AluBI AGCT 2 cut(s) 368, 673
AluI AGCT 2 cut(s) 368, 673
Alw21I GWGCWC 1 cut(s) 636
AlwI GGATC 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 3 cut(s) 86, 292, 563
ApaI GGGCCC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 359
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 86, 87
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 505, 629
AvaI CYCGRG 1 cut(s) 683
BaeGI GKGCMC 2 cut(s) 90, 179
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 636
BccI CCATC 2 cut(s) 139, 667
BciT130I CCWGG 3 cut(s) 109, 421, 562
BcnI CCSGG 1 cut(s) 615
BfaI CTAG 1 cut(s) 645
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
Bme1390I CCNGG 4 cut(s) 109, 421, 562, 615
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 2 cut(s) 86, 87
BmiI GGNNCC 2 cut(s) 6, 88
BmrFI CCNGG 4 cut(s) 109, 421, 562, 615
BmsI GCATC 3 cut(s) 101, 335, 534
BpiI GAAGAC 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 615
BsaJI CCNNGG 6 cut(s) 107, 108, 123, 303, 419, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse118I RCCGGY 1 cut(s) 565
Bse1I ACTGG 3 cut(s) 15, 199, 295
BseBI CCWGG 3 cut(s) 109, 421, 562
BseDI CCNNGG 6 cut(s) 107, 108, 123, 303, 419, 466
BseGI GGATG 4 cut(s) 92, 283, 350, 659
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 3 cut(s) 15, 199, 295
BseSI GKGCMC 2 cut(s) 90, 179
BshFI GGCC 3 cut(s) 88, 294, 565
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 2 cut(s) 566, 615
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 702
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 3 cut(s) 88, 294, 565
BsoBI CYCGRG 1 cut(s) 683
Bsp120I GGGCCC 1 cut(s) 86
Bsp1286I GDGCHC 3 cut(s) 90, 179, 636
Bsp143I GATC 2 cut(s) 513, 619
BspACI CCGC 5 cut(s) 90, 113, 295, 491, 656
BspANI GGCC 3 cut(s) 88, 294, 565
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 6, 88
BspPI GGATC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 565
BsrI ACTGG 3 cut(s) 15, 199, 295
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 6 cut(s) 107, 108, 123, 303, 419, 466
BssMI GATC 2 cut(s) 513, 619
BssT1I CCWWGG 3 cut(s) 123, 303, 466
Bst2UI CCWGG 3 cut(s) 109, 421, 562
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 90, 130
BstDEI CTNAG 2 cut(s) 156, 438
BstF5I GGATG 4 cut(s) 92, 283, 350, 659
BstKTI GATC 2 cut(s) 516, 622
BstMBI GATC 2 cut(s) 513, 619
BstNI CCWGG 3 cut(s) 109, 421, 562
BstSCI CCNGG 4 cut(s) 107, 419, 560, 613
BstSLI GKGCMC 2 cut(s) 90, 179
BstV2I GAAGAC 1 cut(s) 600
BsuRI GGCC 3 cut(s) 88, 294, 565
BtsCI GGATG 4 cut(s) 92, 283, 350, 659
BtsIMutI CAGTG 2 cut(s) 8, 487
Cac8I GCNNGC 2 cut(s) 90, 130
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 2 cut(s) 86, 87
CviAII CATG 2 cut(s) 151, 624
DdeI CTNAG 2 cut(s) 156, 438
DpnI GATC 2 cut(s) 515, 621
DpnII GATC 2 cut(s) 513, 619
EaeI YGGCCR 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 1 cut(s) 645
Eco130I CCWWGG 3 cut(s) 123, 303, 466
Eco24I GRGCYC 1 cut(s) 90
Eco32I GATATC 2 cut(s) 34, 653
Eco88I CYCGRG 1 cut(s) 683
EcoRII CCWGG 3 cut(s) 107, 419, 560
EcoRV GATATC 2 cut(s) 34, 653
EcoT14I CCWWGG 3 cut(s) 123, 303, 466
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 3 cut(s) 123, 303, 466
FaeI CATG 2 cut(s) 154, 627
FaqI GGGAC 1 cut(s) 702
FatI CATG 2 cut(s) 150, 623
FauI CCCGC 1 cut(s) 97
FblI GTMKAC 1 cut(s) 191
Fnu4HI GCNGC 1 cut(s) 295
FokI GGATG 4 cut(s) 79, 290, 357, 646
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 295
FspBI CTAG 1 cut(s) 645
GluI GCNGC 1 cut(s) 295
HaeIII GGCC 3 cut(s) 88, 294, 565
HapII CCGG 2 cut(s) 566, 615
Hin1II CATG 2 cut(s) 154, 627
HinfI GANTC 3 cut(s) 14, 452, 503
HpaII CCGG 2 cut(s) 566, 615
HphI GGTGA 2 cut(s) 505, 629
Hpy166II GTNNAC 3 cut(s) 73, 192, 496
Hpy188I TCNGA 1 cut(s) 508
Hpy188III TCNNGA 3 cut(s) 476, 601, 663
Hpy8I GTNNAC 3 cut(s) 73, 192, 496
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 2 cut(s) 103, 547
HpyF3I CTNAG 2 cut(s) 156, 438
Hsp92II CATG 2 cut(s) 154, 627
Kzo9I GATC 2 cut(s) 513, 619
LmnI GCTCC 3 cut(s) 4, 699, 727
LweI GCATC 3 cut(s) 101, 335, 534
MaeI CTAG 1 cut(s) 645
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 515, 621
MboI GATC 2 cut(s) 513, 619
MhlI GDGCHC 3 cut(s) 90, 179, 636
MluCI AATT 4 cut(s) 98, 198, 359, 445
MnlI CCTC 5 cut(s) 226, 349, 481, 679, 692
MseI TTAA 2 cut(s) 552, 573
MspI CCGG 2 cut(s) 566, 615
MspR9I CCNGG 4 cut(s) 109, 421, 562, 615
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 3 cut(s) 109, 421, 562
NciI CCSGG 1 cut(s) 615
NdeII GATC 2 cut(s) 513, 619
NlaIII CATG 2 cut(s) 154, 627
NlaIV GGNNCC 2 cut(s) 6, 88
PaeR7I CTCGAG 1 cut(s) 683
PasI CCCWGGG 1 cut(s) 108
PctI GAATGC 1 cut(s) 702
PfeI GAWTC 3 cut(s) 14, 452, 503
PkrI GCNGC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 107, 419, 560
PspGI CCWGG 3 cut(s) 107, 419, 560
PspN4I GGNNCC 2 cut(s) 6, 88
PspOMI GGGCCC 1 cut(s) 86
PspPI GGNCC 2 cut(s) 86, 87
PspXI VCTCGAGB 1 cut(s) 683
SaqAI TTAA 2 cut(s) 552, 573
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 2 cut(s) 513, 619
Sau96I GGNCC 2 cut(s) 86, 87
ScrFI CCNGG 4 cut(s) 109, 421, 562, 615
SduI GDGCHC 3 cut(s) 90, 179, 636
SetI ASST 6 cut(s) 162, 219, 370, 472, 521, 675
SfaNI GCATC 3 cut(s) 101, 335, 534
Sfr274I CTCGAG 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 683
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 4 cut(s) 98, 198, 359, 445
SsiI CCGC 5 cut(s) 90, 113, 295, 491, 656
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 4 cut(s) 107, 419, 560, 613
StyI CCWWGG 3 cut(s) 123, 303, 466
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 3 cut(s) 45, 364, 684
TasI AATT 4 cut(s) 98, 198, 359, 445
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 3 cut(s) 14, 452, 503
Tru1I TTAA 2 cut(s) 552, 573
Tru9I TTAA 2 cut(s) 552, 573
TscAI CASTG 2 cut(s) 15, 487
TspDTI ATGAA 2 cut(s) 443, 570
TspRI CASTG 2 cut(s) 15, 487
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 1 cut(s) 359
XhoI CTCGAG 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 191
XspI CTAG 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.