Rw0G023700

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01098
OG0002829
Physical Location & Seq
Reverse (-)
16623 .. 19573
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023700.1

Sequence Viewer

Length: 750 bp
ATGGAGGCACTGGATTCTTTGGGAGAGCCTGATATCAATGATGTCGAAGAAGAAGAAGAAGAAGAAGAAGAACAAGTTCACTATGCTTCTGGGCCCGCATCCAAATTGCAACCCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAGAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTGGTGGCAACCTCTATTACTCCATTGAGGAAGTTCTGTTTTTGGTTGAAATAGGGGCTTTAGTTCTATTGGATGAGAGTGATACTGGCCTTTCCTTGGAAGACGTATATGTGAAGAAGTCAGGTGGCAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCCGACAGTGATGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTCCCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGGGGATATCCGCCATCCAGAGCAGAGCTTGAAGCCCTCGAGGGACAATGTAGGAGCATTCCTTTGAAGTTTTGTAATGTGGAGCAGGGACGGGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

249

Amino Acids

27.77

Weight (kDa)

4.7

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 41 - 93 2.3e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 686
AccI GTMKAC 1 cut(s) 197
AciI CCGC 4 cut(s) 96, 119, 494, 659
AclWI GGATC 1 cut(s) 617
AcsI RAATTY 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 260, 541, 680, 715
AjnI CCWGG 3 cut(s) 113, 422, 563
AluBI AGCT 2 cut(s) 371, 676
AluI AGCT 2 cut(s) 371, 676
Alw21I GWGCWC 1 cut(s) 639
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 686
AoxI GGCC 4 cut(s) 92, 298, 566, 744
ApaI GGGCCC 1 cut(s) 96
ApoI RAATTY 1 cut(s) 362
AseI ATTAAT 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 92, 93
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 2 cut(s) 508, 632
AvaI CYCGRG 1 cut(s) 686
BaeGI GKGCMC 2 cut(s) 96, 185
BanII GRGCYC 1 cut(s) 96
BbsI GAAGAC 2 cut(s) 318, 603
Bbv12I GWGCWC 1 cut(s) 639
BccI CCATC 2 cut(s) 145, 670
BciT130I CCWGG 3 cut(s) 115, 424, 565
BcnI CCSGG 1 cut(s) 618
BfaI CTAG 1 cut(s) 648
Bme1390I CCNGG 4 cut(s) 115, 424, 565, 618
BmeT110I CYCGRG 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 92, 93
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 4 cut(s) 115, 424, 565, 618
BmsI GCATC 3 cut(s) 107, 338, 537
BpiI GAAGAC 2 cut(s) 318, 603
Bpu10I CCTNAGC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 618
BsaJI CCNNGG 6 cut(s) 113, 114, 129, 306, 422, 469
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 3 cut(s) 15, 205, 301
BseBI CCWGG 3 cut(s) 115, 424, 565
BseDI CCNNGG 6 cut(s) 113, 114, 129, 306, 422, 469
BseGI GGATG 4 cut(s) 98, 289, 353, 662
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 3 cut(s) 15, 205, 301
BseSI GKGCMC 2 cut(s) 96, 185
BshFI GGCC 4 cut(s) 94, 300, 568, 746
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 2 cut(s) 569, 618
BslFI GGGAC 2 cut(s) 462, 705
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 2 cut(s) 462, 705
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 4 cut(s) 94, 300, 568, 746
BsoBI CYCGRG 1 cut(s) 686
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 3 cut(s) 96, 185, 639
Bsp143I GATC 2 cut(s) 516, 622
BspACI CCGC 4 cut(s) 96, 119, 494, 659
BspANI GGCC 4 cut(s) 94, 300, 568, 746
BspCNI CTCAG 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 94
BspPI GGATC 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 3 cut(s) 15, 205, 301
BssAI RCCGGY 1 cut(s) 568
BssECI CCNNGG 6 cut(s) 113, 114, 129, 306, 422, 469
BssMI GATC 2 cut(s) 516, 622
BssT1I CCWWGG 3 cut(s) 129, 306, 469
Bst2UI CCWGG 3 cut(s) 115, 424, 565
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 507
BstC8I GCNNGC 2 cut(s) 96, 136
BstDEI CTNAG 2 cut(s) 162, 441
BstF5I GGATG 4 cut(s) 98, 289, 353, 662
BstKTI GATC 2 cut(s) 519, 625
BstMBI GATC 2 cut(s) 516, 622
BstNI CCWGG 3 cut(s) 115, 424, 565
BstSCI CCNGG 4 cut(s) 113, 422, 563, 616
BstSLI GKGCMC 2 cut(s) 96, 185
BstV2I GAAGAC 2 cut(s) 318, 603
BsuRI GGCC 4 cut(s) 94, 300, 568, 746
BtsCI GGATG 4 cut(s) 98, 289, 353, 662
BtsIMutI CAGTG 2 cut(s) 8, 490
Cac8I GCNNGC 2 cut(s) 96, 136
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 2 cut(s) 92, 93
CviAII CATG 2 cut(s) 157, 627
DdeI CTNAG 2 cut(s) 162, 441
DpnI GATC 2 cut(s) 518, 624
DpnII GATC 2 cut(s) 516, 622
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 1 cut(s) 648
Eco130I CCWWGG 3 cut(s) 129, 306, 469
Eco147I AGGCCT 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 96
Eco32I GATATC 2 cut(s) 34, 656
Eco88I CYCGRG 1 cut(s) 686
EcoRII CCWGG 3 cut(s) 113, 422, 563
EcoRV GATATC 2 cut(s) 34, 656
EcoT14I CCWWGG 3 cut(s) 129, 306, 469
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 3 cut(s) 129, 306, 469
FaeI CATG 2 cut(s) 160, 630
FaiI YATR 9 cut(s) 84, 158, 319, 321, 345, 375, 414, 431, 628
FaqI GGGAC 2 cut(s) 462, 705
FatI CATG 2 cut(s) 156, 626
FauI CCCGC 1 cut(s) 103
FblI GTMKAC 1 cut(s) 197
FokI GGATG 4 cut(s) 85, 296, 360, 649
FriOI GRGCYC 1 cut(s) 96
FspBI CTAG 1 cut(s) 648
HaeIII GGCC 4 cut(s) 94, 300, 568, 746
HapII CCGG 2 cut(s) 569, 618
Hin1II CATG 2 cut(s) 160, 630
HinfI GANTC 3 cut(s) 14, 455, 506
HpaII CCGG 2 cut(s) 569, 618
HphI GGTGA 2 cut(s) 508, 632
Hpy166II GTNNAC 3 cut(s) 79, 198, 499
Hpy188I TCNGA 2 cut(s) 177, 511
Hpy188III TCNNGA 3 cut(s) 479, 604, 666
Hpy8I GTNNAC 3 cut(s) 79, 198, 499
HpyAV CCTTC 2 cut(s) 172, 430
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 109, 550
HpyF3I CTNAG 2 cut(s) 162, 441
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 2 cut(s) 160, 630
Kzo9I GATC 2 cut(s) 516, 622
LmnI GCTCC 2 cut(s) 702, 730
LweI GCATC 3 cut(s) 107, 338, 537
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 395
MalI GATC 2 cut(s) 518, 624
MboI GATC 2 cut(s) 516, 622
MhlI GDGCHC 3 cut(s) 96, 185, 639
MluCI AATT 4 cut(s) 104, 204, 362, 448
MnlI CCTC 7 cut(s) 232, 233, 352, 484, 682, 695, 736
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 2 cut(s) 555, 576
MspI CCGG 2 cut(s) 569, 618
MspR9I CCNGG 4 cut(s) 115, 424, 565, 618
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 3 cut(s) 115, 424, 565
NciI CCSGG 1 cut(s) 618
NdeII GATC 2 cut(s) 516, 622
NlaIII CATG 2 cut(s) 160, 630
NlaIV GGNNCC 1 cut(s) 94
PaeR7I CTCGAG 1 cut(s) 686
PasI CCCWGGG 1 cut(s) 114
PceI AGGCCT 1 cut(s) 746
PctI GAATGC 1 cut(s) 705
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 3 cut(s) 14, 455, 506
PshBI ATTAAT 1 cut(s) 555
Psp6I CCWGG 3 cut(s) 113, 422, 563
PspGI CCWGG 3 cut(s) 113, 422, 563
PspN4I GGNNCC 1 cut(s) 94
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 92, 93
PspXI VCTCGAGB 1 cut(s) 686
SaqAI TTAA 2 cut(s) 555, 576
Sau3AI GATC 2 cut(s) 516, 622
Sau96I GGNCC 2 cut(s) 92, 93
ScrFI CCNGG 4 cut(s) 115, 424, 565, 618
SduI GDGCHC 3 cut(s) 96, 185, 639
SetI ASST 8 cut(s) 168, 225, 318, 337, 373, 475, 524, 678
SfaNI GCATC 3 cut(s) 107, 338, 537
Sfr274I CTCGAG 1 cut(s) 686
SlaI CTCGAG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 4 cut(s) 104, 204, 362, 448
SseBI AGGCCT 1 cut(s) 746
SsiI CCGC 4 cut(s) 96, 119, 494, 659
SspMI CTAG 1 cut(s) 648
StuI AGGCCT 1 cut(s) 746
StyD4I CCNGG 4 cut(s) 113, 422, 563, 616
StyI CCWWGG 3 cut(s) 129, 306, 469
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 318
TaqI TCGA 3 cut(s) 45, 367, 687
TasI AATT 4 cut(s) 104, 204, 362, 448
TfiI GAWTC 3 cut(s) 14, 455, 506
Tru1I TTAA 2 cut(s) 555, 576
Tru9I TTAA 2 cut(s) 555, 576
TscAI CASTG 2 cut(s) 15, 490
TspDTI ATGAA 2 cut(s) 446, 573
TspRI CASTG 2 cut(s) 15, 490
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 1 cut(s) 362
XhoI CTCGAG 1 cut(s) 686
XmiI GTMKAC 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 75
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.