MD14G1076600.v1.1

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002829
Physical Location & Seq
Reverse (-)
8678058 .. 8680741
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1076600.v1.1.491

Sequence Viewer

Length: 810 bp
ATGAAAAAGAAAATTAGAAAACAAACCTTTTTTTGTTCCCCCTTTACCAACTCTTCCTCCGCCTCGCCCCCGCGCAGTGTCTTCCTCCACCCCACCTTTTTCAGTCTCTTCCCCGCCCACCACCCCCCCCCCCCCGGCGTTTATATATCCTCTTCCGACCCGAGCTTATCTCTTCCTCCCTCACCGCCCCTTTTTCCTCCCTCCCCCCGAAACCCTCTCACTGTCTTCCATCCATTAACGAGGTTGAAATTTGGTATAATGGAGGTCGTGGACCGGGAGAAGAAGAAGAAGATCAATGACATTGATGAAGGAGAAGATCACTATGCTTCTGTGTCTGCTTCCAAGTCGCAATTCAGGAAAGTCATATTGAAGGCTCGCTGGAGTGATGAGATGGGTATGGCTGAGGTTGTAGATAAAAAGGGTGCAATTTGGAGGACCACGGGAATTGTTCGTAGTGGCAAGCTCTATTTCTTCATTGAGGAAGTTTTATATGGTGGCCTTGATAGCGATGAGGAGGTGGATTTGGATTCAGAAGAGTCTAGGTCTGTCATTGGATTGTTCAATGATATGCAAATTAATGAAACAAGGCTGGTATTTGATGTTTATCTCCCAAACAGCAAGTTCAGGAAATCTTCTCCTGGTGACCCAAGTTTTGTGCTCTGCTTTACCAGAGGTCATCCACCACCCAAAGCAGACCTAGAAGCCCTCGAGAGACGATGCGATAACATTCCCATGAAAGTTTGTCATGTGGAGGAGGGGGTTGTGAGTTTCTTTTCCTTTGACAAAGTGGAACTTCCTATCCTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

270

Amino Acids

30.38

Weight (kDa)

7.05

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 110 - 164 1.2e-08 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 73
AciI CCGC 4 cut(s) 60, 71, 114, 185
AcsI RAATTY 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 3 cut(s) 247, 370, 562
AjnI CCWGG 1 cut(s) 637
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AloI GAACNNNNNNTCC 1 cut(s) 783
AluBI AGCT 2 cut(s) 165, 463
AluI AGCT 2 cut(s) 165, 463
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 2 cut(s) 110, 706
Ama87I CYCGRG 2 cut(s) 160, 707
AoxI GGCC 1 cut(s) 496
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 635, 667
AseI ATTAAT 1 cut(s) 576
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 271, 435
AsuC2I CCSGG 2 cut(s) 135, 275
AsuHPI GGTGA 2 cut(s) 174, 653
AvaI CYCGRG 2 cut(s) 160, 707
AvaII GGWCC 2 cut(s) 271, 435
BbsI GAAGAC 2 cut(s) 73, 217
Bbv12I GWGCWC 1 cut(s) 660
BbvCI CCTCAGC 1 cut(s) 402
BccI CCATC 2 cut(s) 237, 385
BciT130I CCWGG 1 cut(s) 639
BcnI CCSGG 2 cut(s) 135, 275
BcoDI GTCTC 2 cut(s) 110, 706
BfaI CTAG 2 cut(s) 540, 698
Bme1390I CCNGG 3 cut(s) 135, 275, 639
Bme18I GGWCC 2 cut(s) 271, 435
BmeT110I CYCGRG 2 cut(s) 160, 707
BmgT120I GGNCC 2 cut(s) 271, 435
BmrFI CCNGG 3 cut(s) 135, 275, 639
BmsI GCATC 1 cut(s) 707
BpiI GAAGAC 2 cut(s) 73, 217
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 402
BpuMI CCSGG 2 cut(s) 135, 275
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 2 cut(s) 133, 438
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 133, 438
BseGI GGATG 2 cut(s) 229, 676
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 393
BseRI GAGGAG 2 cut(s) 527, 767
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 660
BsiHKCI CYCGRG 2 cut(s) 160, 707
BsiSI CCGG 2 cut(s) 135, 274
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 110, 706
BsmBI CGTCTC 1 cut(s) 706
BsnI GGCC 1 cut(s) 498
BsoBI CYCGRG 2 cut(s) 160, 707
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 2 cut(s) 291, 316
BspACI CCGC 4 cut(s) 60, 71, 114, 185
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 133, 438
BssMI GATC 2 cut(s) 291, 316
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 5 cut(s) 58, 113, 157, 177, 528
BstC8I GCNNGC 2 cut(s) 376, 461
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 438
BstEII GGTNACC 1 cut(s) 641
BstF5I GGATG 2 cut(s) 229, 676
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 294, 319
BstMAI GTCTC 2 cut(s) 110, 706
BstMBI GATC 2 cut(s) 291, 316
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstNI CCWGG 1 cut(s) 639
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 3 cut(s) 133, 273, 637
BstUI CGCG 1 cut(s) 73
BstV2I GAAGAC 2 cut(s) 73, 217
BsuRI GGCC 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 2 cut(s) 229, 676
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 82, 219
Cac8I GCNNGC 2 cut(s) 376, 461
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 271, 435
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 2 cut(s) 733, 746
CviJI RGCY 7 cut(s) 165, 374, 401, 463, 498, 589, 704
CviKI_1 RGCY 7 cut(s) 165, 374, 401, 463, 498, 589, 704
DdeI CTNAG 1 cut(s) 402
DpnI GATC 2 cut(s) 293, 318
DpnII GATC 2 cut(s) 291, 316
Eam1104I CTCTTC 5 cut(s) 58, 113, 157, 177, 528
EarI CTCTTC 5 cut(s) 58, 113, 157, 177, 528
EciI GGCGGA 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 271, 435
Eco88I CYCGRG 2 cut(s) 160, 707
Eco91I GGTNACC 1 cut(s) 641
EcoO65I GGTNACC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 706
FaeI CATG 2 cut(s) 736, 749
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FatI CATG 2 cut(s) 732, 745
FauI CCCGC 2 cut(s) 78, 121
FokI GGATG 2 cut(s) 216, 663
FspBI CTAG 2 cut(s) 540, 698
GlaI GCGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 400
HaeIII GGCC 1 cut(s) 498
HapII CCGG 2 cut(s) 135, 274
HhaI GCGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 736, 749
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 2 cut(s) 527, 536
HpaII CCGG 2 cut(s) 135, 274
HphI GGTGA 2 cut(s) 174, 653
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 157, 532
Hpy188III TCNNGA 3 cut(s) 355, 625, 709
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 302, 364
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 2 cut(s) 425, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 1 cut(s) 402
Hsp92II CATG 2 cut(s) 736, 749
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 2 cut(s) 291, 316
LpnPI CCDG 9 cut(s) 148, 287, 340, 364, 575, 610, 624, 651, 682
LweI GCATC 1 cut(s) 707
MaeI CTAG 2 cut(s) 540, 698
MaeIII GTNAC 1 cut(s) 641
MalI GATC 2 cut(s) 293, 318
MboI GATC 2 cut(s) 291, 316
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 6 cut(s) 12, 248, 350, 426, 444, 573
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 1 cut(s) 180
MseI TTAA 2 cut(s) 236, 576
MslI CAYNNNNRTG 1 cut(s) 731
MspI CCGG 2 cut(s) 135, 274
MspR9I CCNGG 3 cut(s) 135, 275, 639
MvaI CCWGG 1 cut(s) 639
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 504
NciI CCSGG 2 cut(s) 135, 275
NdeII GATC 2 cut(s) 291, 316
NlaIII CATG 2 cut(s) 736, 749
NmuCI GTSAC 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 707
PfeI GAWTC 1 cut(s) 527
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 637
PspEI GGTNACC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 637
PspPI GGNCC 2 cut(s) 271, 435
RseI CAYNNNNRTG 1 cut(s) 731
SaqAI TTAA 2 cut(s) 236, 576
Sau3AI GATC 2 cut(s) 291, 316
Sau96I GGNCC 2 cut(s) 271, 435
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 3 cut(s) 135, 275, 639
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 1 cut(s) 707
Sfr274I CTCGAG 1 cut(s) 707
SinI GGWCC 2 cut(s) 271, 435
SlaI CTCGAG 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 6 cut(s) 12, 248, 350, 426, 444, 573
SsiI CCGC 4 cut(s) 60, 71, 114, 185
SspMI CTAG 2 cut(s) 540, 698
StyD4I CCNGG 3 cut(s) 133, 273, 637
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 1 cut(s) 708
TasI AATT 6 cut(s) 12, 248, 350, 426, 444, 573
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 2 cut(s) 236, 576
Tru9I TTAA 2 cut(s) 236, 576
TscAI CASTG 2 cut(s) 82, 226
TseFI GTSAC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 5 cut(s) 17, 321, 463, 594, 749
TspRI CASTG 2 cut(s) 82, 226
VpaK11BI GGWCC 2 cut(s) 271, 435
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 707
XspI CTAG 2 cut(s) 540, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.