RchiOBHm_Chr3g0483371

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002829
Physical Location & Seq
Reverse (-)
29560823 .. 29563763
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44812

Sequence Viewer

Length: 840 bp
ATGGAGGCACTGGATTCTTTGGGAGAGCCTAATATCAATGATGTCGAAGAAGAAGAAGAAGAAGAAGTTCACTATGCTTCTGGGCCCGCATCCAAATTGCAACCCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAGAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTAGTGGCAACCTCTATTACTCCATTGAGGAAGTTCTGTTTTTGGTTGAAATTGGGGCTTTAGTTCTCTTGGATGAGAGTGATACTGGCCTTTCCTTGGAAGATGTATATGTGAATATGTCAGGTGGCAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCTGACAGTGACGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTACCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGCTTCATGCAAACATATGAAGCCAATTCTTTCGCAAAGGTTTTGCAGAGTAGCAGCAAACGGGGATATCCGCCATCCAGAGCAGAGCTTGAAGCCCTCGAGGGACAATGTAGGAGCATTCCTTTGAAGTTTTGTAATGTGGAGCAGGGACGGGTGAGTTTCTTTTCCTTTGACAAGGTTGAACTTCCTATCCTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

279

Amino Acids

31.17

Weight (kDa)

4.76

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 39 - 90 2e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 737
AccI GTMKAC 1 cut(s) 188
AciI CCGC 4 cut(s) 87, 110, 485, 710
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 5 cut(s) 251, 532, 731, 766, 821
AjnI CCWGG 3 cut(s) 104, 413, 554
AloI GAACNNNNNNTCC 1 cut(s) 813
AluBI AGCT 3 cut(s) 362, 642, 727
AluI AGCT 3 cut(s) 362, 642, 727
Alw21I GWGCWC 1 cut(s) 630
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 3 cut(s) 83, 289, 557
ApaI GGGCCC 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 693
ApoI RAATTY 1 cut(s) 353
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 83, 84
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 3 cut(s) 499, 623, 805
AvaI CYCGRG 1 cut(s) 737
BaeGI GKGCMC 2 cut(s) 87, 176
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 705
BccI CCATC 2 cut(s) 136, 721
BciT130I CCWGG 3 cut(s) 106, 415, 556
BcnI CCSGG 1 cut(s) 609
BfaI CTAG 2 cut(s) 639, 833
BisI GCNGC 1 cut(s) 694
BlsI GCNGC 1 cut(s) 695
Bme1390I CCNGG 4 cut(s) 106, 415, 556, 609
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 83, 84
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 4 cut(s) 106, 415, 556, 609
BmsI GCATC 3 cut(s) 98, 329, 528
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 609
BsaJI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 3 cut(s) 15, 196, 292
BseBI CCWGG 3 cut(s) 106, 415, 556
BseDI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
BseGI GGATG 4 cut(s) 89, 280, 344, 713
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 3 cut(s) 15, 196, 292
BseSI GKGCMC 2 cut(s) 87, 176
BseXI GCAGC 1 cut(s) 705
BshFI GGCC 3 cut(s) 85, 291, 559
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 2 cut(s) 560, 609
BslFI GGGAC 2 cut(s) 756, 801
BslI CCNNNNNNNGG 1 cut(s) 298
BsmFI GGGAC 2 cut(s) 756, 801
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 3 cut(s) 85, 291, 559
BsoBI CYCGRG 1 cut(s) 737
Bsp120I GGGCCC 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 87, 176, 630
Bsp143I GATC 2 cut(s) 507, 613
BspACI CCGC 4 cut(s) 87, 110, 485, 710
BspANI GGCC 3 cut(s) 85, 291, 559
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 3 cut(s) 15, 196, 292
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
BssMI GATC 2 cut(s) 507, 613
BssT1I CCWWGG 3 cut(s) 120, 297, 460
Bst2UI CCWGG 3 cut(s) 106, 415, 556
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 87, 127
BstDEI CTNAG 2 cut(s) 153, 432
BstF5I GGATG 4 cut(s) 89, 280, 344, 713
BstKTI GATC 2 cut(s) 510, 616
BstMBI GATC 2 cut(s) 507, 613
BstNI CCWGG 3 cut(s) 106, 415, 556
BstSCI CCNGG 4 cut(s) 104, 413, 554, 607
BstSLI GKGCMC 2 cut(s) 87, 176
BstV1I GCAGC 1 cut(s) 705
BstV2I GAAGAC 1 cut(s) 594
BsuRI GGCC 3 cut(s) 85, 291, 559
BtsCI GGATG 4 cut(s) 89, 280, 344, 713
BtsIMutI CAGTG 2 cut(s) 8, 481
Cac8I GCNNGC 2 cut(s) 87, 127
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 2 cut(s) 83, 84
CviAII CATG 3 cut(s) 148, 618, 646
DdeI CTNAG 2 cut(s) 153, 432
DpnI GATC 2 cut(s) 509, 615
DpnII GATC 2 cut(s) 507, 613
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 699
Eco130I CCWWGG 3 cut(s) 120, 297, 460
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 737
EcoRII CCWGG 3 cut(s) 104, 413, 554
EcoRV GATATC 1 cut(s) 707
EcoT14I CCWWGG 3 cut(s) 120, 297, 460
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 120, 297, 460
FaeI CATG 3 cut(s) 151, 621, 649
FaqI GGGAC 2 cut(s) 756, 801
FatI CATG 3 cut(s) 147, 617, 645
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 655
FblI GTMKAC 1 cut(s) 188
Fnu4HI GCNGC 1 cut(s) 694
FokI GGATG 4 cut(s) 76, 287, 351, 700
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 1 cut(s) 694
FspBI CTAG 2 cut(s) 639, 833
GluI GCNGC 1 cut(s) 694
HaeIII GGCC 3 cut(s) 85, 291, 559
HapII CCGG 2 cut(s) 560, 609
Hin1II CATG 3 cut(s) 151, 621, 649
HinfI GANTC 3 cut(s) 14, 446, 497
HpaII CCGG 2 cut(s) 560, 609
HphI GGTGA 3 cut(s) 499, 623, 805
Hpy166II GTNNAC 3 cut(s) 70, 189, 490
Hpy188I TCNGA 2 cut(s) 168, 502
Hpy188III TCNNGA 3 cut(s) 470, 595, 717
Hpy8I GTNNAC 3 cut(s) 70, 189, 490
HpyAV CCTTC 2 cut(s) 163, 421
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4V TGCA 4 cut(s) 100, 541, 649, 685
HpyF3I CTNAG 2 cut(s) 153, 432
Hsp92II CATG 3 cut(s) 151, 621, 649
Kzo9I GATC 2 cut(s) 507, 613
LmnI GCTCC 2 cut(s) 753, 781
Lsp1109I GCAGC 1 cut(s) 705
LweI GCATC 3 cut(s) 98, 329, 528
MaeI CTAG 2 cut(s) 639, 833
MaeIII GTNAC 2 cut(s) 386, 476
MalI GATC 2 cut(s) 509, 615
MboI GATC 2 cut(s) 507, 613
MhlI GDGCHC 3 cut(s) 87, 176, 630
MluCI AATT 6 cut(s) 95, 195, 252, 353, 439, 664
MnlI CCTC 6 cut(s) 223, 224, 343, 475, 733, 746
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 546, 567
MspI CCGG 2 cut(s) 560, 609
MspR9I CCNGG 4 cut(s) 106, 415, 556, 609
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 3 cut(s) 106, 415, 556
NciI CCSGG 1 cut(s) 609
NdeI CATATG 1 cut(s) 655
NdeII GATC 2 cut(s) 507, 613
NlaIII CATG 3 cut(s) 151, 621, 649
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 476
PaeR7I CTCGAG 1 cut(s) 737
PasI CCCWGGG 1 cut(s) 105
PctI GAATGC 1 cut(s) 756
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 3 cut(s) 14, 446, 497
PkrI GCNGC 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 546
Psp6I CCWGG 3 cut(s) 104, 413, 554
PspGI CCWGG 3 cut(s) 104, 413, 554
PspN4I GGNNCC 1 cut(s) 85
PspOMI GGGCCC 1 cut(s) 83
PspPI GGNCC 2 cut(s) 83, 84
PspXI VCTCGAGB 1 cut(s) 737
SaqAI TTAA 2 cut(s) 546, 567
SatI GCNGC 1 cut(s) 694
Sau3AI GATC 2 cut(s) 507, 613
Sau96I GGNCC 2 cut(s) 83, 84
ScrFI CCNGG 4 cut(s) 106, 415, 556, 609
SduI GDGCHC 3 cut(s) 87, 176, 630
SfaNI GCATC 3 cut(s) 98, 329, 528
Sfr274I CTCGAG 1 cut(s) 737
SlaI CTCGAG 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
Sse9I AATT 6 cut(s) 95, 195, 252, 353, 439, 664
SsiI CCGC 4 cut(s) 87, 110, 485, 710
SspMI CTAG 2 cut(s) 639, 833
StyD4I CCNGG 4 cut(s) 104, 413, 554, 607
StyI CCWWGG 3 cut(s) 120, 297, 460
TaaI ACNGT 1 cut(s) 476
TaqI TCGA 3 cut(s) 45, 358, 738
TasI AATT 6 cut(s) 95, 195, 252, 353, 439, 664
TfiI GAWTC 3 cut(s) 14, 446, 497
Tru1I TTAA 2 cut(s) 546, 567
Tru9I TTAA 2 cut(s) 546, 567
TscAI CASTG 2 cut(s) 15, 481
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 693
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 4 cut(s) 437, 564, 634, 672
TspRI CASTG 2 cut(s) 15, 481
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 737
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 2 cut(s) 639, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.