Rh3AG253800

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002829
Physical Location & Seq
Reverse (-)
27609292 .. 27611919
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG253800.1

Sequence Viewer

Length: 606 bp
ATGTTTCAGGGTTCTGATTTTGATGTTGGCGGCTCTTTCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAGAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTAGTGGCAACCTCTATTACTCCATTGAGGAAGTTCTGTTTTTGGTTGAAATTGGGGCTTTAGTTCTCTTGGATGAGAGTGATACTGGCCTTTCCTTGGAAGATGTATATGTGAATATGTCAGGTGGCAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCTGACAGTGACGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTACCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGGTATTTCTTCTGGTTACTTGAAAGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

201

Amino Acids

22.69

Weight (kDa)

5.06

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 17 - 68 1.4e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 122
AciI CCGC 3 cut(s) 30, 44, 419
AclWI GGATC 1 cut(s) 542
AcsI RAATTY 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 3 cut(s) 185, 466, 596
AjnI CCWGG 2 cut(s) 347, 488
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw21I GWGCWC 1 cut(s) 564
AlwI GGATC 1 cut(s) 542
AoxI GGCC 2 cut(s) 223, 491
ApoI RAATTY 1 cut(s) 287
AseI ATTAAT 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 433, 557
BaeGI GKGCMC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 564
BccI CCATC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 349, 490
BcnI CCSGG 1 cut(s) 543
BfaI CTAG 1 cut(s) 573
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
Bme1390I CCNGG 3 cut(s) 349, 490, 543
BmrFI CCNGG 3 cut(s) 349, 490, 543
BmsI GCATC 2 cut(s) 263, 462
BpiI GAAGAC 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 543
BsaJI CCNNGG 4 cut(s) 54, 231, 347, 394
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 130, 226
BseBI CCWGG 2 cut(s) 349, 490
BseDI CCNNGG 4 cut(s) 54, 231, 347, 394
BseGI GGATG 3 cut(s) 214, 278, 606
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 130, 226
BseSI GKGCMC 1 cut(s) 110
BshFI GGCC 2 cut(s) 225, 493
BsiHKAI GWGCWC 1 cut(s) 564
BsiSI CCGG 2 cut(s) 494, 543
BslI CCNNNNNNNGG 1 cut(s) 232
BsnI GGCC 2 cut(s) 225, 493
Bsp1286I GDGCHC 2 cut(s) 110, 564
Bsp143I GATC 2 cut(s) 441, 547
BspACI CCGC 3 cut(s) 30, 44, 419
BspANI GGCC 2 cut(s) 225, 493
BspCNI CTCAG 1 cut(s) 100
BspPI GGATC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 493
BsrI ACTGG 2 cut(s) 130, 226
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 4 cut(s) 54, 231, 347, 394
BssMI GATC 2 cut(s) 441, 547
BssT1I CCWWGG 3 cut(s) 54, 231, 394
Bst2UI CCWGG 2 cut(s) 349, 490
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 2 cut(s) 87, 366
BstF5I GGATG 3 cut(s) 214, 278, 606
BstKTI GATC 2 cut(s) 444, 550
BstMBI GATC 2 cut(s) 441, 547
BstNI CCWGG 2 cut(s) 349, 490
BstSCI CCNGG 3 cut(s) 347, 488, 541
BstSLI GKGCMC 1 cut(s) 110
BstV2I GAAGAC 1 cut(s) 528
BsuRI GGCC 2 cut(s) 225, 493
BtsCI GGATG 3 cut(s) 214, 278, 606
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 493
CviAII CATG 2 cut(s) 82, 552
CviJI RGCY 9 cut(s) 33, 59, 86, 194, 225, 296, 365, 488, 493
CviKI_1 RGCY 9 cut(s) 33, 59, 86, 194, 225, 296, 365, 488, 493
DdeI CTNAG 2 cut(s) 87, 366
DpnI GATC 2 cut(s) 443, 549
DpnII GATC 2 cut(s) 441, 547
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco130I CCWWGG 3 cut(s) 54, 231, 394
EcoRII CCWGG 2 cut(s) 347, 488
EcoT14I CCWWGG 3 cut(s) 54, 231, 394
ErhI CCWWGG 3 cut(s) 54, 231, 394
FaeI CATG 2 cut(s) 85, 555
FaiI YATR 9 cut(s) 83, 244, 246, 254, 270, 300, 339, 356, 553
FatI CATG 2 cut(s) 81, 551
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 2 cut(s) 221, 285
Fsp4HI GCNGC 1 cut(s) 31
FspBI CTAG 1 cut(s) 573
GluI GCNGC 1 cut(s) 31
HaeIII GGCC 2 cut(s) 225, 493
HapII CCGG 2 cut(s) 494, 543
Hin1II CATG 2 cut(s) 85, 555
HinfI GANTC 2 cut(s) 380, 431
HpaII CCGG 2 cut(s) 494, 543
HphI GGTGA 2 cut(s) 433, 557
Hpy166II GTNNAC 2 cut(s) 123, 424
Hpy188I TCNGA 3 cut(s) 16, 102, 436
Hpy188III TCNNGA 2 cut(s) 404, 529
Hpy8I GTNNAC 2 cut(s) 123, 424
HpyAV CCTTC 2 cut(s) 97, 355
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 1 cut(s) 475
HpyF3I CTNAG 2 cut(s) 87, 366
Hsp92II CATG 2 cut(s) 85, 555
Kzo9I GATC 2 cut(s) 441, 547
LweI GCATC 2 cut(s) 263, 462
MaeI CTAG 1 cut(s) 573
MaeIII GTNAC 3 cut(s) 320, 410, 588
MalI GATC 2 cut(s) 443, 549
MboI GATC 2 cut(s) 441, 547
MboII GAAGA 6 cut(s) 248, 370, 381, 449, 528, 574
MhlI GDGCHC 2 cut(s) 110, 564
MluCI AATT 4 cut(s) 129, 186, 287, 373
MnlI CCTC 4 cut(s) 157, 158, 277, 409
MseI TTAA 2 cut(s) 480, 501
MspI CCGG 2 cut(s) 494, 543
MspR9I CCNGG 3 cut(s) 349, 490, 543
MvaI CCWGG 2 cut(s) 349, 490
NciI CCSGG 1 cut(s) 543
NdeII GATC 2 cut(s) 441, 547
NlaIII CATG 2 cut(s) 85, 555
NmuCI GTSAC 1 cut(s) 410
PfeI GAWTC 2 cut(s) 380, 431
PkrI GCNGC 1 cut(s) 32
PshBI ATTAAT 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 347, 488
PspGI CCWGG 2 cut(s) 347, 488
SaqAI TTAA 2 cut(s) 480, 501
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 2 cut(s) 441, 547
ScrFI CCNGG 3 cut(s) 349, 490, 543
SduI GDGCHC 2 cut(s) 110, 564
SetI ASST 8 cut(s) 93, 150, 262, 298, 400, 449, 517, 578
SfaNI GCATC 2 cut(s) 263, 462
Sse9I AATT 4 cut(s) 129, 186, 287, 373
SsiI CCGC 3 cut(s) 30, 44, 419
SspMI CTAG 1 cut(s) 573
StyD4I CCNGG 3 cut(s) 347, 488, 541
StyI CCWWGG 3 cut(s) 54, 231, 394
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 292
TasI AATT 4 cut(s) 129, 186, 287, 373
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 380, 431
Tru1I TTAA 2 cut(s) 480, 501
Tru9I TTAA 2 cut(s) 480, 501
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 2 cut(s) 371, 498
TspRI CASTG 1 cut(s) 415
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 122
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.