Rh3CG287900

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002829
Physical Location & Seq
Reverse (-)
29148369 .. 29150849
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG287900.1

Sequence Viewer

Length: 717 bp
ATGGAGGCACTGGATTCTTTGGGAGAGCCTGATATCAATGATGTCGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACAAGTTCACTATGCTTCTGGGCCCGCATCCAAATTGCAACCCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAGAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTGGTGGCAACCTCTATTACTCCATTGAGGAAGTTCTGTTTTTGGTTGAAATAGGGGCTTTAGTTCTATTGGATGAGAGTGATACTGGCCTTTCCTTGGAAGACGTATATGTGAAGAAGTCAGGTGGCAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCCGACAGTGATGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTCCCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGCTTCATGCAAACATATGAAGCCAATTCTTTCGCAAAGGTTTTGCAGAGTAGCAGCAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

238

Amino Acids

26.57

Weight (kDa)

4.64

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 42 - 94 2.1e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 200
AciI CCGC 3 cut(s) 99, 122, 497
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 1 cut(s) 365
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 2 cut(s) 263, 544
AjnI CCWGG 3 cut(s) 116, 425, 566
AluBI AGCT 2 cut(s) 374, 654
AluI AGCT 2 cut(s) 374, 654
Alw21I GWGCWC 1 cut(s) 642
AlwI GGATC 1 cut(s) 620
AoxI GGCC 3 cut(s) 95, 301, 569
ApaI GGGCCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 705
ApoI RAATTY 1 cut(s) 365
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 78
AspS9I GGNCC 2 cut(s) 95, 96
AsuC2I CCSGG 1 cut(s) 621
AsuHPI GGTGA 2 cut(s) 511, 635
BaeGI GKGCMC 2 cut(s) 99, 188
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 321, 606
Bbv12I GWGCWC 1 cut(s) 642
BccI CCATC 1 cut(s) 148
BciT130I CCWGG 3 cut(s) 118, 427, 568
BcnI CCSGG 1 cut(s) 621
BfaI CTAG 1 cut(s) 651
BisI GCNGC 1 cut(s) 706
BlsI GCNGC 1 cut(s) 707
Bme1390I CCNGG 4 cut(s) 118, 427, 568, 621
BmgT120I GGNCC 2 cut(s) 95, 96
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 4 cut(s) 118, 427, 568, 621
BmsI GCATC 3 cut(s) 110, 341, 540
BpiI GAAGAC 2 cut(s) 321, 606
Bpu10I CCTNAGC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 621
BsaJI CCNNGG 6 cut(s) 116, 117, 132, 309, 425, 472
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 571
Bse1I ACTGG 3 cut(s) 15, 208, 304
BseBI CCWGG 3 cut(s) 118, 427, 568
BseDI CCNNGG 6 cut(s) 116, 117, 132, 309, 425, 472
BseGI GGATG 3 cut(s) 101, 292, 356
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 3 cut(s) 15, 208, 304
BseSI GKGCMC 2 cut(s) 99, 188
BshFI GGCC 3 cut(s) 97, 303, 571
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 2 cut(s) 572, 621
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 465
BsnI GGCC 3 cut(s) 97, 303, 571
Bsp120I GGGCCC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 99, 188, 642
Bsp143I GATC 2 cut(s) 519, 625
BspACI CCGC 3 cut(s) 99, 122, 497
BspANI GGCC 3 cut(s) 97, 303, 571
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 571
BsrI ACTGG 3 cut(s) 15, 208, 304
BssAI RCCGGY 1 cut(s) 571
BssECI CCNNGG 6 cut(s) 116, 117, 132, 309, 425, 472
BssMI GATC 2 cut(s) 519, 625
BssT1I CCWWGG 3 cut(s) 132, 309, 472
Bst2UI CCWGG 3 cut(s) 118, 427, 568
Bst4CI ACNGT 2 cut(s) 488, 714
Bst6I CTCTTC 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 99, 139
BstDEI CTNAG 2 cut(s) 165, 444
BstF5I GGATG 3 cut(s) 101, 292, 356
BstKTI GATC 2 cut(s) 522, 628
BstMBI GATC 2 cut(s) 519, 625
BstNI CCWGG 3 cut(s) 118, 427, 568
BstSCI CCNGG 4 cut(s) 116, 425, 566, 619
BstSLI GKGCMC 2 cut(s) 99, 188
BstV2I GAAGAC 2 cut(s) 321, 606
BsuRI GGCC 3 cut(s) 97, 303, 571
BtsCI GGATG 3 cut(s) 101, 292, 356
BtsIMutI CAGTG 2 cut(s) 8, 493
Cac8I GCNNGC 2 cut(s) 99, 139
Cfr10I RCCGGY 1 cut(s) 571
Cfr13I GGNCC 2 cut(s) 95, 96
CviAII CATG 3 cut(s) 160, 630, 658
DdeI CTNAG 2 cut(s) 165, 444
DpnI GATC 2 cut(s) 521, 627
DpnII GATC 2 cut(s) 519, 625
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco130I CCWWGG 3 cut(s) 132, 309, 472
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 34
EcoRII CCWGG 3 cut(s) 116, 425, 566
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 3 cut(s) 132, 309, 472
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 3 cut(s) 132, 309, 472
FaeI CATG 3 cut(s) 163, 633, 661
FaqI GGGAC 1 cut(s) 465
FatI CATG 3 cut(s) 159, 629, 657
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 667
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 1 cut(s) 706
FokI GGATG 3 cut(s) 88, 299, 363
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 706
FspBI CTAG 1 cut(s) 651
GluI GCNGC 1 cut(s) 706
HaeIII GGCC 3 cut(s) 97, 303, 571
HapII CCGG 2 cut(s) 572, 621
Hin1II CATG 3 cut(s) 163, 633, 661
HinfI GANTC 3 cut(s) 14, 458, 509
HpaII CCGG 2 cut(s) 572, 621
HphI GGTGA 2 cut(s) 511, 635
Hpy166II GTNNAC 3 cut(s) 82, 201, 502
Hpy188I TCNGA 2 cut(s) 180, 514
Hpy188III TCNNGA 2 cut(s) 482, 607
Hpy8I GTNNAC 3 cut(s) 82, 201, 502
HpyAV CCTTC 2 cut(s) 175, 433
HpyCH4III ACNGT 2 cut(s) 488, 714
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 112, 553, 661, 697
HpyF3I CTNAG 2 cut(s) 165, 444
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 3 cut(s) 163, 633, 661
Kzo9I GATC 2 cut(s) 519, 625
LweI GCATC 3 cut(s) 110, 341, 540
MaeI CTAG 1 cut(s) 651
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 398
MalI GATC 2 cut(s) 521, 627
MboI GATC 2 cut(s) 519, 625
MhlI GDGCHC 3 cut(s) 99, 188, 642
MluCI AATT 5 cut(s) 107, 207, 365, 451, 676
MnlI CCTC 4 cut(s) 235, 236, 355, 487
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 2 cut(s) 558, 579
MspI CCGG 2 cut(s) 572, 621
MspR9I CCNGG 4 cut(s) 118, 427, 568, 621
MvaI CCWGG 3 cut(s) 118, 427, 568
NciI CCSGG 1 cut(s) 621
NdeI CATATG 1 cut(s) 667
NdeII GATC 2 cut(s) 519, 625
NlaIII CATG 3 cut(s) 163, 633, 661
NlaIV GGNNCC 1 cut(s) 97
PasI CCCWGGG 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 14, 458, 509
PkrI GCNGC 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 558
Psp6I CCWGG 3 cut(s) 116, 425, 566
PspGI CCWGG 3 cut(s) 116, 425, 566
PspN4I GGNNCC 1 cut(s) 97
PspOMI GGGCCC 1 cut(s) 95
PspPI GGNCC 2 cut(s) 95, 96
SaqAI TTAA 2 cut(s) 558, 579
SatI GCNGC 1 cut(s) 706
Sau3AI GATC 2 cut(s) 519, 625
Sau96I GGNCC 2 cut(s) 95, 96
ScrFI CCNGG 4 cut(s) 118, 427, 568, 621
SduI GDGCHC 3 cut(s) 99, 188, 642
SetI ASST 9 cut(s) 171, 228, 321, 340, 376, 478, 527, 656, 693
SfaNI GCATC 3 cut(s) 110, 341, 540
Sse9I AATT 5 cut(s) 107, 207, 365, 451, 676
SsiI CCGC 3 cut(s) 99, 122, 497
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 4 cut(s) 116, 425, 566, 619
StyI CCWWGG 3 cut(s) 132, 309, 472
TaaI ACNGT 2 cut(s) 488, 714
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 45, 370
TasI AATT 5 cut(s) 107, 207, 365, 451, 676
TfiI GAWTC 3 cut(s) 14, 458, 509
Tru1I TTAA 2 cut(s) 558, 579
Tru9I TTAA 2 cut(s) 558, 579
TscAI CASTG 2 cut(s) 15, 493
TseI GCWGC 1 cut(s) 705
TspDTI ATGAA 4 cut(s) 449, 576, 646, 684
TspRI CASTG 2 cut(s) 15, 493
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.