AT3G57360

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002829
Physical Location & Seq
Forward (+)
21225540 .. 21227039
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57360.1

Sequence Viewer

Length: 765 bp
ATGGAGGAAAAAGACTGGGAAGCTTCTTCTTCGAGCGAAAACGAAGGTGGTTTTCCAAACGATGATGACGAAGAGTTTCATTCTGGTGGTTCAGTGCCCAAGTTACAGTTCAGAGTAGGATCTTCCAAGGCTCGTTGGATTACTGAACTAGGAATGGCGGAAGTTGAGGTTAAAAGGGGAAAACTTTGGACGACAACTGGAATCATTCGTAGTGGAAAGACATATTGCTTCATAGAAGAGGCTCTGTATTTGAGTGAAATTGGAGAGTTACAGATCTTAGGTAATGAGGATGACATAGTGATCCCATTGAAGGACTTGTATGAGAAAATTGCAGAGGAGAAAAGCGGTTGCTCTTGGGAAAACTACGAGGTTTATAGATACTTGAAGGGTCTAGGTTACATCCTAGGTAGGCATGGAGTTTCTTGGACGTTAAAGGATGCTGCAAGACCGAATGGTGAAGAAGAGTCAGCTTGTGCTGGTGAATGTCCTGCAGACAATGATACTGTAACTAAACTCTTGGGAGATATGCAGATTTGTGATGCAAAAGCGGTGTTTGACGTGTATCTACCAAACAGCCGGTTCAAGAAGTCTTCTCCTGGTGAACCAAGCTTTGTGGCTTGCTTCTCGGGGGACTCTCCACCAAGCAAAGAAGATATTAAAGTCCTGCAAAAGCGCATAGCTGCACCATTAACATTCTGTCATATCGCTCAGGGCCGTGCCAGTTTCTTTTCCTTCAGTTCTATAGACCTCCCTGTCTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

254

Amino Acids

28.11

Weight (kDa)

4.76

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 31 - 92 1.3e-13 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 752
AciI CCGC 3 cut(s) 158, 345, 548
AclWI GGATC 2 cut(s) 127, 295
AcuI CTGAAG 1 cut(s) 718
AfiI CCNNNNNNNGG 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 3 cut(s) 310, 385, 583
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 595
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 4 cut(s) 23, 470, 609, 680
AluI AGCT 4 cut(s) 23, 470, 609, 680
AlwI GGATC 2 cut(s) 127, 295
Ama87I CYCGRG 1 cut(s) 625
AoxI GGCC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 440, 680
Asp700I GAANNNNTTC 1 cut(s) 75
AspA2I CCTAGG 1 cut(s) 403
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 1 cut(s) 712
AsuHPI GGTGA 3 cut(s) 467, 491, 611
AvaI CYCGRG 1 cut(s) 625
AvrII CCTAGG 1 cut(s) 403
BaeGI GKGCMC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 2 cut(s) 427, 667
BceAI ACGGC 1 cut(s) 699
BciT130I CCWGG 1 cut(s) 597
BfaI CTAG 3 cut(s) 149, 392, 404
BfmI CTRYAG 2 cut(s) 489, 741
BglII AGATCT 1 cut(s) 273
BisI GCNGC 2 cut(s) 441, 681
BlnI CCTAGG 1 cut(s) 403
BlsI GCNGC 2 cut(s) 442, 682
Bme1390I CCNGG 1 cut(s) 597
BmeT110I CYCGRG 1 cut(s) 625
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 712
BmrFI CCNGG 1 cut(s) 597
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 2 cut(s) 427, 529
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 708
BsaJI CCNNGG 2 cut(s) 126, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 576
Bse1I ACTGG 3 cut(s) 20, 202, 720
BseBI CCWGG 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 126, 403
BseGI GGATG 3 cut(s) 295, 399, 442
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 3 cut(s) 20, 202, 720
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 2 cut(s) 427, 667
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 625
BsiSI CCGG 1 cut(s) 577
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 1 cut(s) 714
BsoBI CYCGRG 1 cut(s) 625
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 119, 273, 300
BspACI CCGC 3 cut(s) 158, 345, 548
BspANI GGCC 1 cut(s) 714
BspCNI CTCAG 1 cut(s) 721
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 127, 295
BsrFI RCCGGY 1 cut(s) 576
BsrI ACTGG 3 cut(s) 20, 202, 720
BssAI RCCGGY 1 cut(s) 576
BssECI CCNNGG 2 cut(s) 126, 403
BssMI GATC 3 cut(s) 119, 273, 300
BssT1I CCWWGG 2 cut(s) 126, 403
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 2 cut(s) 108, 505
Bst6I CTCTTC 3 cut(s) 66, 231, 456
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 2 cut(s) 277, 708
BstF5I GGATG 3 cut(s) 295, 399, 442
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 3 cut(s) 122, 276, 303
BstMBI GATC 3 cut(s) 119, 273, 300
BstNI CCWGG 1 cut(s) 597
BstSCI CCNGG 1 cut(s) 595
BstSFI CTRYAG 2 cut(s) 489, 741
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 427, 667
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 119, 273
BstYI RGATCY 2 cut(s) 119, 273
BsuRI GGCC 1 cut(s) 714
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 3 cut(s) 295, 399, 442
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 619
CfoI GCGC 1 cut(s) 675
Cfr10I RCCGGY 1 cut(s) 576
Cfr13I GGNCC 1 cut(s) 712
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 1 cut(s) 413
CviJI RGCY 9 cut(s) 23, 131, 242, 470, 576, 609, 617, 680, 714
CviKI_1 RGCY 9 cut(s) 23, 131, 242, 470, 576, 609, 617, 680, 714
DdeI CTNAG 2 cut(s) 277, 708
DpnI GATC 3 cut(s) 121, 275, 302
DpnII GATC 3 cut(s) 119, 273, 300
DrdI GACNNNNNNGTC 1 cut(s) 752
DseDI GACNNNNNNGTC 1 cut(s) 752
Eam1104I CTCTTC 3 cut(s) 66, 231, 456
EarI CTCTTC 3 cut(s) 66, 231, 456
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 126, 403
Eco57I CTGAAG 1 cut(s) 718
Eco88I CYCGRG 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 595
EcoT14I CCWWGG 2 cut(s) 126, 403
ErhI CCWWGG 2 cut(s) 126, 403
FaeI CATG 1 cut(s) 416
FaqI GGGAC 1 cut(s) 644
FatI CATG 1 cut(s) 412
Fnu4HI GCNGC 2 cut(s) 441, 681
FokI GGATG 3 cut(s) 302, 386, 449
Fsp4HI GCNGC 2 cut(s) 441, 681
FspBI CTAG 3 cut(s) 149, 392, 404
GlaI GCGC 1 cut(s) 674
GluI GCNGC 2 cut(s) 441, 681
HaeIII GGCC 1 cut(s) 714
HapII CCGG 1 cut(s) 577
HhaI GCGC 1 cut(s) 675
Hin1II CATG 1 cut(s) 416
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HindIII AAGCTT 2 cut(s) 21, 607
HinfI GANTC 3 cut(s) 201, 464, 632
HpaII CCGG 1 cut(s) 577
HphI GGTGA 3 cut(s) 467, 491, 611
Hpy166II GTNNAC 1 cut(s) 602
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 1 cut(s) 583
Hpy8I GTNNAC 1 cut(s) 602
HpyAV CCTTC 4 cut(s) 38, 304, 379, 742
HpyCH4III ACNGT 2 cut(s) 108, 505
HpyCH4IV ACGT 2 cut(s) 428, 558
HpyCH4V TGCA 7 cut(s) 332, 443, 491, 529, 542, 667, 683
HpyF3I CTNAG 2 cut(s) 277, 708
HpySE526I ACGT 2 cut(s) 428, 558
Hsp92II CATG 1 cut(s) 416
HspAI GCGC 1 cut(s) 673
Kzo9I GATC 3 cut(s) 119, 273, 300
Lsp1109I GCAGC 2 cut(s) 427, 667
LweI GCATC 2 cut(s) 427, 529
MaeI CTAG 3 cut(s) 149, 392, 404
MaeII ACGT 2 cut(s) 428, 558
MaeIII GTNAC 4 cut(s) 102, 267, 395, 505
MalI GATC 3 cut(s) 121, 275, 302
MboI GATC 3 cut(s) 119, 273, 300
MboII GAAGA 9 cut(s) 18, 21, 83, 114, 248, 470, 473, 582, 662
MflI RGATCY 2 cut(s) 119, 273
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 2 cut(s) 258, 327
MlyI GAGTC 2 cut(s) 473, 626
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 6 cut(s) 160, 232, 280, 328, 361, 758
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 4 cut(s) 171, 431, 657, 689
MslI CAYNNNNRTG 1 cut(s) 84
MspI CCGG 1 cut(s) 577
MspR9I CCNGG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 597
NdeII GATC 3 cut(s) 119, 273, 300
NlaIII CATG 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 2 cut(s) 442, 682
PleI GAGTC 2 cut(s) 472, 626
PpsI GAGTC 2 cut(s) 472, 626
Psp6I CCWGG 1 cut(s) 595
PspGI CCWGG 1 cut(s) 595
PspPI GGNCC 1 cut(s) 712
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 2 cut(s) 119, 273
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 4 cut(s) 171, 431, 657, 689
SatI GCNGC 2 cut(s) 441, 681
Sau3AI GATC 3 cut(s) 119, 273, 300
Sau96I GGNCC 1 cut(s) 712
SchI GAGTC 2 cut(s) 473, 626
ScrFI CCNGG 1 cut(s) 597
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 2 cut(s) 427, 529
SfcI CTRYAG 2 cut(s) 489, 741
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 2 cut(s) 258, 327
SsiI CCGC 3 cut(s) 158, 345, 548
SspMI CTAG 3 cut(s) 149, 392, 404
StyD4I CCNGG 1 cut(s) 595
StyI CCWWGG 2 cut(s) 126, 403
TaaI ACNGT 2 cut(s) 108, 505
TaiI ACGT 2 cut(s) 431, 561
TaqI TCGA 1 cut(s) 32
TaqII GACCGA 1 cut(s) 463
TasI AATT 2 cut(s) 258, 327
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 4 cut(s) 171, 431, 657, 689
Tru9I TTAA 4 cut(s) 171, 431, 657, 689
TscAI CASTG 1 cut(s) 99
TseI GCWGC 2 cut(s) 440, 680
TspDTI ATGAA 2 cut(s) 68, 220
TspRI CASTG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 75
XspI CTAG 3 cut(s) 149, 392, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.