Rh3DG283700

tRNA-splicing endonuclease subunit sen54 N-term

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002829
Physical Location & Seq
Reverse (-)
28909928 .. 28912444
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG283700.1

Sequence Viewer

Length: 738 bp
ATGGAGGCACTGGATTCTTTGGGAGAGCCTGATATCAATGATGTCGAAGAAGAAGAAGAAGAACAAGTTCACTATGCTTCTGGGCCCGCATCCAAATTGCAACCCAGGGCGGTTGTGTCCAAGGCTCGCTGGAATGATGAGATGGGCATGGCTCAGGTCGTAGTTCAGAAGGGCACATTTTCCAAGTCTACTGGAATTGTTCGTAGTGGCAACCTCTATTACTCCATTGAGGAAGTTCTGTTTTTGGTTGAAATAGGGGCTTTAGTTCTCTTGGATGAGAATGATACTGGCCTTTCCTTGGAAGACGTATATGTAAAGATGTCAGGTGGCAAGTATGGATGCTGTTGGGAGGAATTTCGAGCTTATAGGCAACTCAAATCTCTTGGTTACATTGTTGGGCGTTATGGTATTCCCTGGTCTATGAAGAAGGCTAAGAGTAATTGTGAATCTATTTCTTCCCAAGGTTGTCCCGACAGTGATGAGGCGGTGAACTTGGAATCAGAAGAGATCAGGTCTGTCATTGGATTGTTCAATGAGATGCAGATTAATGAAGCCAGGCCGGTTTTTAATGTGTATCTCCCTAACAGCAAGTTCAGGAAGTCTTCTCCCGGTGATCCATGCTTTGTGCTCTGTTTTACTAGGGGATATCCGCCATCCAGAGCAGAGCTTGAAGCCCTCGAGGGACAATGTAGGAGCATTCCTTTGAAGTTTTGTAATGTGGAGCAGGGACGGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_int_end_N2

Protein Analysis

245

Amino Acids

27.3

Weight (kDa)

4.79

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 38 - 90 1.5e-11 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 677
AccI GTMKAC 1 cut(s) 188
AciI CCGC 4 cut(s) 87, 110, 485, 650
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 298
AgsI TTSAA 4 cut(s) 251, 532, 671, 706
AjnI CCWGG 3 cut(s) 104, 413, 554
AluBI AGCT 2 cut(s) 362, 667
AluI AGCT 2 cut(s) 362, 667
Alw21I GWGCWC 1 cut(s) 630
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 677
AoxI GGCC 3 cut(s) 83, 289, 557
ApaI GGGCCC 1 cut(s) 87
ApoI RAATTY 1 cut(s) 353
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 83, 84
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 2 cut(s) 499, 623
AvaI CYCGRG 1 cut(s) 677
BaeGI GKGCMC 2 cut(s) 87, 176
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 309, 594
Bbv12I GWGCWC 1 cut(s) 630
BccI CCATC 2 cut(s) 136, 661
BciT130I CCWGG 3 cut(s) 106, 415, 556
BcnI CCSGG 1 cut(s) 609
BfaI CTAG 1 cut(s) 639
Bme1390I CCNGG 4 cut(s) 106, 415, 556, 609
BmeT110I CYCGRG 1 cut(s) 677
BmgT120I GGNCC 2 cut(s) 83, 84
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 4 cut(s) 106, 415, 556, 609
BmsI GCATC 3 cut(s) 98, 329, 528
BpiI GAAGAC 2 cut(s) 309, 594
Bpu10I CCTNAGC 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 609
BsaJI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 3 cut(s) 15, 196, 292
BseBI CCWGG 3 cut(s) 106, 415, 556
BseDI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
BseGI GGATG 4 cut(s) 89, 280, 344, 653
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 3 cut(s) 15, 196, 292
BseSI GKGCMC 2 cut(s) 87, 176
BshFI GGCC 3 cut(s) 85, 291, 559
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 2 cut(s) 560, 609
BslFI GGGAC 2 cut(s) 453, 696
BslI CCNNNNNNNGG 1 cut(s) 298
BsmFI GGGAC 2 cut(s) 453, 696
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 3 cut(s) 85, 291, 559
BsoBI CYCGRG 1 cut(s) 677
Bsp120I GGGCCC 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 87, 176, 630
Bsp143I GATC 2 cut(s) 507, 613
BspACI CCGC 4 cut(s) 87, 110, 485, 650
BspANI GGCC 3 cut(s) 85, 291, 559
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 3 cut(s) 15, 196, 292
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 6 cut(s) 104, 105, 120, 297, 413, 460
BssMI GATC 2 cut(s) 507, 613
BssT1I CCWWGG 3 cut(s) 120, 297, 460
Bst2UI CCWGG 3 cut(s) 106, 415, 556
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 87, 127
BstDEI CTNAG 2 cut(s) 153, 432
BstF5I GGATG 4 cut(s) 89, 280, 344, 653
BstKTI GATC 2 cut(s) 510, 616
BstMBI GATC 2 cut(s) 507, 613
BstNI CCWGG 3 cut(s) 106, 415, 556
BstSCI CCNGG 4 cut(s) 104, 413, 554, 607
BstSLI GKGCMC 2 cut(s) 87, 176
BstV2I GAAGAC 2 cut(s) 309, 594
BsuRI GGCC 3 cut(s) 85, 291, 559
BtsCI GGATG 4 cut(s) 89, 280, 344, 653
BtsIMutI CAGTG 2 cut(s) 8, 481
Cac8I GCNNGC 2 cut(s) 87, 127
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 2 cut(s) 83, 84
CviAII CATG 2 cut(s) 148, 618
DdeI CTNAG 2 cut(s) 153, 432
DpnI GATC 2 cut(s) 509, 615
DpnII GATC 2 cut(s) 507, 613
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 639
Eco130I CCWWGG 3 cut(s) 120, 297, 460
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 2 cut(s) 34, 647
Eco88I CYCGRG 1 cut(s) 677
EcoRII CCWGG 3 cut(s) 104, 413, 554
EcoRV GATATC 2 cut(s) 34, 647
EcoT14I CCWWGG 3 cut(s) 120, 297, 460
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 120, 297, 460
FaeI CATG 2 cut(s) 151, 621
FaiI YATR 9 cut(s) 75, 149, 310, 312, 336, 366, 405, 422, 619
FaqI GGGAC 2 cut(s) 453, 696
FatI CATG 2 cut(s) 147, 617
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 1 cut(s) 188
FokI GGATG 4 cut(s) 76, 287, 351, 640
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 1 cut(s) 639
HaeIII GGCC 3 cut(s) 85, 291, 559
HapII CCGG 2 cut(s) 560, 609
Hin1II CATG 2 cut(s) 151, 621
HinfI GANTC 3 cut(s) 14, 446, 497
HpaII CCGG 2 cut(s) 560, 609
HphI GGTGA 2 cut(s) 499, 623
Hpy166II GTNNAC 3 cut(s) 70, 189, 490
Hpy188I TCNGA 2 cut(s) 168, 502
Hpy188III TCNNGA 3 cut(s) 470, 595, 657
Hpy8I GTNNAC 3 cut(s) 70, 189, 490
HpyAV CCTTC 2 cut(s) 163, 421
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 2 cut(s) 100, 541
HpyF3I CTNAG 2 cut(s) 153, 432
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 151, 621
Kzo9I GATC 2 cut(s) 507, 613
LmnI GCTCC 2 cut(s) 693, 721
LweI GCATC 3 cut(s) 98, 329, 528
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 386
MalI GATC 2 cut(s) 509, 615
MboI GATC 2 cut(s) 507, 613
MhlI GDGCHC 3 cut(s) 87, 176, 630
MluCI AATT 4 cut(s) 95, 195, 353, 439
MnlI CCTC 6 cut(s) 223, 224, 343, 475, 673, 686
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 546, 567
MspI CCGG 2 cut(s) 560, 609
MspR9I CCNGG 4 cut(s) 106, 415, 556, 609
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 3 cut(s) 106, 415, 556
NciI CCSGG 1 cut(s) 609
NdeII GATC 2 cut(s) 507, 613
NlaIII CATG 2 cut(s) 151, 621
NlaIV GGNNCC 1 cut(s) 85
PaeR7I CTCGAG 1 cut(s) 677
PasI CCCWGGG 1 cut(s) 105
PctI GAATGC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 3 cut(s) 14, 446, 497
PshBI ATTAAT 1 cut(s) 546
Psp6I CCWGG 3 cut(s) 104, 413, 554
PspGI CCWGG 3 cut(s) 104, 413, 554
PspN4I GGNNCC 1 cut(s) 85
PspOMI GGGCCC 1 cut(s) 83
PspPI GGNCC 2 cut(s) 83, 84
PspXI VCTCGAGB 1 cut(s) 677
SaqAI TTAA 2 cut(s) 546, 567
Sau3AI GATC 2 cut(s) 507, 613
Sau96I GGNCC 2 cut(s) 83, 84
ScrFI CCNGG 4 cut(s) 106, 415, 556, 609
SduI GDGCHC 3 cut(s) 87, 176, 630
SetI ASST 8 cut(s) 159, 216, 309, 328, 364, 466, 515, 669
SfaNI GCATC 3 cut(s) 98, 329, 528
Sfr274I CTCGAG 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 4 cut(s) 95, 195, 353, 439
SsiI CCGC 4 cut(s) 87, 110, 485, 650
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 4 cut(s) 104, 413, 554, 607
StyI CCWWGG 3 cut(s) 120, 297, 460
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 309
TaqI TCGA 3 cut(s) 45, 358, 678
TasI AATT 4 cut(s) 95, 195, 353, 439
TfiI GAWTC 3 cut(s) 14, 446, 497
Tru1I TTAA 2 cut(s) 546, 567
Tru9I TTAA 2 cut(s) 546, 567
TscAI CASTG 2 cut(s) 15, 481
TspDTI ATGAA 2 cut(s) 437, 564
TspRI CASTG 2 cut(s) 15, 481
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.