AT3G12100

metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005497
Physical Location & Seq
Reverse (-)
3854487 .. 3857319
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12100.2

Sequence Viewer

Length: 1182 bp
ATGGAGAGATCCATTTCCTTTAATCCTCGAGGCGATAACGAGCTCCCTGATGATCGGAGCAGCGATGTCGGATATGCTGCTAATGATCGGAGGCTTGCTTATTCTCGCTCGTTTCAGCAATCTCACGGTCCACGAACGCCGGCGGTCACGGAAGCGGCTAAGCCTTTTCTCGATAGGACGGTCTCGAGCATCGATATGCCGCCGGATATTTACTCCGTTGATGGAAGTGATGTGTTTTTCGGGGAAGGGAAAGATGTAGACATGGCAAAAGTGTCTGTTTTGGAAATGGTTTGGGAGGTTTTTGGAGTAGTGACAAGTGGAAATCGGCAGATGAAGAGACTGTTTCTGCTAATTGCGCTTAATGTGTTGTATTCTACAACAGAGCTCTCTATTGGTATCTTCACTGGTCGTGTAGGTTTGGTCTCTGATGCATTCCATTTGACATTTGGTTGTGGTCTCTTGACATTTTCTTTATTTGCAATGGCAACTTCAAGGAAGAAGCCTGATCATGCTTACTCATACGGGTACAAAAGACTTGAAGTTTTATCTGCTTTCACTAATGCTCTGTTCCTTATGTTCATGTCATTCTCCTTAGCTGTGGAAGCTCTTCATGCATTTATACAAGATGAATCAGAGCATAAGCATTATTTGATTGTATCAGCGGTAACAAATCTGCTGGTGAACCTACTAGGTGTTTGGTTTTTCCGGAATTATGCTCGTGTCAATATTGCCTACAGAAAAGCAGAAGATATGAATTATCACTCCGTTTGCTTGCACGTCATATCAGATTCCATCCGCAGTGCAGGTTTGATACTGGCATCTTGGCTCCTCTCGCTCGGGGTTGAAAATGCAGAGGTCCTATGTTTAGGATTGGTATCGGTCACAGTGTTTATGCTTGTTATGCCACTCTTCAAAGCCACTGGTGGTGTCTTGCTTCAGATGGCACCCCCAAACATTCCTTCTTCTGCTTTAAGCAAATGCTTGCGACAGATTACTTCTCGAGAAGATGTAACGGAGGTTTTACAGGCACGTTTCTGGGAGGTTGTGCCTGGTCACACTGTTGGCTCACTCAGACTCCAGGTGAAGAGCGGAATAGATGAAAGACCATTACTGCAATATGTATATGATGTATACCATGATTTGGGTGTACAAGACTTGACCCTGCAAACTGATTACACCTAA

Protein Analysis

393

Amino Acids

43.81

Weight (kDa)

5.82

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 114 - 265 1e-27 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 794
AccB1I GGYRCC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 1141
AccBSI CCGCTC 1 cut(s) 1089
AccI GTMKAC 2 cut(s) 258, 1131
AccIII TCCGGA 1 cut(s) 705
AciI CCGC 6 cut(s) 143, 155, 200, 662, 796, 1089
AclWI GGATC 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 920
AfaI GTAC 2 cut(s) 527, 1149
AfiI CCNNNNNNNGG 1 cut(s) 1141
AgsI TTSAA 4 cut(s) 492, 539, 845, 913
AjiI CACGTC 1 cut(s) 778
AjnI CCWGG 2 cut(s) 1048, 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 943, 975
AluBI AGCT 4 cut(s) 43, 385, 596, 605
AluI AGCT 4 cut(s) 43, 385, 596, 605
Alw21I GWGCWC 2 cut(s) 45, 387
Alw26I GTCTC 4 cut(s) 187, 331, 427, 461
AlwI GGATC 1 cut(s) 3
Ama87I CYCGRG 4 cut(s) 27, 184, 836, 999
Aor13HI TCCGGA 1 cut(s) 705
ApeKI GCWGC 2 cut(s) 60, 77
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 128, 856
AsuHPI GGTGA 2 cut(s) 691, 1093
AvaI CYCGRG 4 cut(s) 27, 184, 836, 999
AvaII GGWCC 2 cut(s) 128, 856
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 2 cut(s) 45, 387
BauI CACGAG 1 cut(s) 717
Bbv12I GWGCWC 2 cut(s) 45, 387
BbvI GCAGC 2 cut(s) 64, 72
BccI CCATC 3 cut(s) 215, 800, 934
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciT130I CCWGG 2 cut(s) 1050, 1079
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 4 cut(s) 187, 331, 427, 461
BfaI CTAG 1 cut(s) 689
BfmI CTRYAG 1 cut(s) 733
BfuAI ACCTGC 1 cut(s) 794
BisI GCNGC 4 cut(s) 61, 78, 156, 200
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 4 cut(s) 62, 79, 157, 201
Bme1390I CCNGG 2 cut(s) 1050, 1079
Bme18I GGWCC 2 cut(s) 128, 856
BmeT110I CYCGRG 4 cut(s) 27, 184, 836, 999
BmgBI CACGTC 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 128, 856
BmiI GGNNCC 2 cut(s) 827, 945
BmrFI CCNGG 2 cut(s) 1050, 1079
BmsI GCATC 3 cut(s) 198, 418, 827
BpmI CTGGAG 1 cut(s) 1061
Bpu10I CCTNAGC 1 cut(s) 592
Bpu1102I GCTNAGC 1 cut(s) 159
Bsa29I ATCGAT 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 874
BsaI GGTCTC 3 cut(s) 187, 427, 461
BsaWI WCCGGW 1 cut(s) 705
BsaXI ACNNNNNCTCC 2 cut(s) 1059, 1089
Bsc4I CCNNNNNNNGG 1 cut(s) 1141
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 3 cut(s) 409, 819, 925
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 874
BseAI TCCGGA 1 cut(s) 705
BseBI CCWGG 2 cut(s) 1050, 1079
BseCI ATCGAT 1 cut(s) 192
BseGI GGATG 1 cut(s) 792
BseJI GATNNNNATC 1 cut(s) 874
BseLI CCNNNNNNNGG 1 cut(s) 1141
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 1084
BseNI ACTGG 3 cut(s) 409, 819, 925
BseRI GAGGAG 1 cut(s) 818
BseXI GCAGC 2 cut(s) 64, 72
BsgI GTGCAG 1 cut(s) 822
BshNI GGYRCC 1 cut(s) 943
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 45, 387
BsiHKCI CYCGRG 4 cut(s) 27, 184, 836, 999
BsiSI CCGG 3 cut(s) 140, 203, 706
BslI CCNNNNNNNGG 1 cut(s) 1141
BsmAI GTCTC 4 cut(s) 187, 331, 427, 461
BsmI GAATGC 1 cut(s) 431
Bso31I GGTCTC 3 cut(s) 187, 427, 461
BsoBI CYCGRG 4 cut(s) 27, 184, 836, 999
Bsp1286I GDGCHC 2 cut(s) 45, 387
Bsp13I TCCGGA 1 cut(s) 705
Bsp1407I TGTACA 1 cut(s) 1147
Bsp143I GATC 4 cut(s) 8, 52, 85, 505
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 6 cut(s) 143, 155, 200, 662, 796, 1089
BspCNI CTCAG 1 cut(s) 1083
BspDI ATCGAT 1 cut(s) 192
BspEI TCCGGA 1 cut(s) 705
BspLI GGNNCC 2 cut(s) 827, 945
BspMI ACCTGC 1 cut(s) 794
BspPI GGATC 1 cut(s) 3
BspQI GCTCTTC 2 cut(s) 612, 1079
BspT107I GGYRCC 1 cut(s) 943
BspTNI GGTCTC 3 cut(s) 187, 427, 461
BsrBI CCGCTC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 1147
BsrI ACTGG 3 cut(s) 409, 819, 925
BssAI RCCGGY 1 cut(s) 139
BssMI GATC 4 cut(s) 8, 52, 85, 505
BssNAI GTATAC 1 cut(s) 1132
BssSI CACGAG 1 cut(s) 717
Bst1107I GTATAC 1 cut(s) 1132
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 1050, 1079
Bst4CI ACNGT 5 cut(s) 128, 181, 342, 886, 1060
Bst6I CTCTTC 4 cut(s) 329, 612, 914, 1079
BstAUI TGTACA 1 cut(s) 1147
BstC8I GCNNGC 4 cut(s) 96, 141, 773, 983
BstDEI CTNAG 3 cut(s) 159, 592, 1070
BstF5I GGATG 1 cut(s) 792
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 4 cut(s) 11, 55, 88, 508
BstMAI GTCTC 4 cut(s) 187, 331, 427, 461
BstMBI GATC 4 cut(s) 8, 52, 85, 505
BstMWI GCNNNNNNNGC 5 cut(s) 355, 602, 611, 832, 901
BstNI CCWGG 2 cut(s) 1050, 1079
BstSCI CCNGG 2 cut(s) 1048, 1077
BstSFI CTRYAG 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 64, 72
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BstZ17I GTATAC 1 cut(s) 1132
Bsu15I ATCGAT 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 778
BtsCI GGATG 1 cut(s) 792
BtsI GCAGTG 1 cut(s) 805
BtsIMutI CAGTG 5 cut(s) 402, 805, 891, 918, 1056
BveI ACCTGC 1 cut(s) 794
Cac8I GCNNGC 4 cut(s) 96, 141, 773, 983
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 2 cut(s) 128, 856
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 2 cut(s) 526, 1148
CviAII CATG 5 cut(s) 262, 509, 580, 611, 1136
CviQI GTAC 2 cut(s) 526, 1148
DdeI CTNAG 3 cut(s) 159, 592, 1070
DpnI GATC 4 cut(s) 10, 54, 87, 507
DpnII GATC 4 cut(s) 8, 52, 85, 505
Eam1104I CTCTTC 4 cut(s) 329, 612, 914, 1079
EarI CTCTTC 4 cut(s) 329, 612, 914, 1079
Ecl136II GAGCTC 2 cut(s) 43, 385
Eco24I GRGCYC 2 cut(s) 45, 387
Eco31I GGTCTC 3 cut(s) 187, 427, 461
Eco47I GGWCC 2 cut(s) 128, 856
Eco53kI GAGCTC 2 cut(s) 43, 385
Eco57I CTGAAG 1 cut(s) 920
Eco88I CYCGRG 4 cut(s) 27, 184, 836, 999
EcoICRI GAGCTC 2 cut(s) 43, 385
EcoO109I RGGNCCY 1 cut(s) 856
EcoRII CCWGG 2 cut(s) 1048, 1077
EcoT22I ATGCAT 2 cut(s) 433, 616
EcoT38I GRGCYC 2 cut(s) 45, 387
FaeI CATG 5 cut(s) 265, 512, 583, 614, 1139
FatI CATG 5 cut(s) 261, 508, 579, 610, 1135
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 2 cut(s) 258, 1131
Fnu4HI GCNGC 4 cut(s) 61, 78, 156, 200
FokI GGATG 1 cut(s) 779
FriOI GRGCYC 2 cut(s) 45, 387
Fsp4HI GCNGC 4 cut(s) 61, 78, 156, 200
FspBI CTAG 1 cut(s) 689
GlaI GCGC 1 cut(s) 357
GluI GCNGC 4 cut(s) 61, 78, 156, 200
GsuI CTGGAG 1 cut(s) 1061
HapII CCGG 3 cut(s) 140, 203, 706
HhaI GCGC 1 cut(s) 358
Hin1II CATG 5 cut(s) 265, 512, 583, 614, 1139
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 3 cut(s) 629, 788, 1074
HpaII CCGG 3 cut(s) 140, 203, 706
HphI GGTGA 2 cut(s) 691, 1093
Hpy166II GTNNAC 5 cut(s) 131, 259, 682, 1132, 1148
Hpy188I TCNGA 8 cut(s) 57, 71, 90, 427, 634, 787, 939, 1073
Hpy188III TCNNGA 6 cut(s) 170, 184, 460, 706, 999, 1001
Hpy8I GTNNAC 5 cut(s) 131, 259, 682, 1132, 1148
HpyAV CCTTC 2 cut(s) 239, 969
HpyCH4III ACNGT 5 cut(s) 128, 181, 342, 886, 1060
HpyCH4IV ACGT 2 cut(s) 777, 1030
HpyCH4V TGCA 8 cut(s) 431, 479, 614, 775, 803, 851, 1114, 1165
HpyF10VI GCNNNNNNNGC 5 cut(s) 355, 602, 611, 832, 901
HpyF3I CTNAG 3 cut(s) 159, 592, 1070
HpySE526I ACGT 2 cut(s) 777, 1030
Hsp92II CATG 5 cut(s) 265, 512, 583, 614, 1139
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 705
KroI GCCGGC 1 cut(s) 139
KroNI GCCGGC 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 4 cut(s) 8, 52, 85, 505
LguI GCTCTTC 2 cut(s) 612, 1079
LmnI GCTCC 3 cut(s) 48, 57, 831
Lsp1109I GCAGC 2 cut(s) 64, 72
LweI GCATC 3 cut(s) 198, 418, 827
MaeI CTAG 1 cut(s) 689
MaeII ACGT 2 cut(s) 777, 1030
MaeIII GTNAC 6 cut(s) 145, 310, 664, 880, 1009, 1052
MalI GATC 4 cut(s) 10, 54, 87, 507
MbiI CCGCTC 1 cut(s) 1089
MboI GATC 4 cut(s) 8, 52, 85, 505
MboII GAAGA 9 cut(s) 346, 391, 508, 599, 758, 901, 954, 1016, 1096
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 45, 387
MluCI AATT 3 cut(s) 351, 709, 754
MlyI GAGTC 1 cut(s) 1068
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 8 cut(s) 23, 36, 84, 289, 839, 847, 1009, 1033
Mph1103I ATGCAT 2 cut(s) 433, 616
MreI CGCCGGCG 1 cut(s) 139
MroI TCCGGA 1 cut(s) 705
MroNI GCCGGC 1 cut(s) 139
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 3 cut(s) 21, 360, 971
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 3 cut(s) 140, 203, 706
MspR9I CCNGG 2 cut(s) 1050, 1079
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 2 cut(s) 1050, 1079
MwoI GCNNNNNNNGC 5 cut(s) 355, 602, 611, 832, 901
NaeI GCCGGC 1 cut(s) 141
NdeII GATC 4 cut(s) 8, 52, 85, 505
NgoMIV GCCGGC 1 cut(s) 139
NlaIII CATG 5 cut(s) 265, 512, 583, 614, 1139
NlaIV GGNNCC 2 cut(s) 827, 945
NmuCI GTSAC 4 cut(s) 145, 310, 880, 1052
NsiI ATGCAT 2 cut(s) 433, 616
PaeR7I CTCGAG 3 cut(s) 27, 184, 999
PciSI GCTCTTC 2 cut(s) 612, 1079
PctI GAATGC 1 cut(s) 431
PdiI GCCGGC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 629, 788
PflMI CCANNNNNTGG 1 cut(s) 1141
PkrI GCNGC 4 cut(s) 62, 79, 157, 201
PleI GAGTC 1 cut(s) 1068
PpsI GAGTC 1 cut(s) 1068
PpuMI RGGWCCY 1 cut(s) 856
Psp124BI GAGCTC 2 cut(s) 45, 387
Psp5II RGGWCCY 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 1048, 1077
PspGI CCWGG 2 cut(s) 1048, 1077
PspN4I GGNNCC 2 cut(s) 827, 945
PspPI GGNCC 2 cut(s) 128, 856
PspPPI RGGWCCY 1 cut(s) 856
PspXI VCTCGAGB 1 cut(s) 27
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 2 cut(s) 527, 1149
RsaNI GTAC 2 cut(s) 526, 1148
RseI CAYNNNNRTG 1 cut(s) 194
SacI GAGCTC 2 cut(s) 45, 387
SapI GCTCTTC 2 cut(s) 612, 1079
SaqAI TTAA 3 cut(s) 21, 360, 971
SatI GCNGC 4 cut(s) 61, 78, 156, 200
Sau3AI GATC 4 cut(s) 8, 52, 85, 505
Sau96I GGNCC 2 cut(s) 128, 856
SchI GAGTC 1 cut(s) 1068
ScrFI CCNGG 2 cut(s) 1050, 1079
SduI GDGCHC 2 cut(s) 45, 387
SfaNI GCATC 3 cut(s) 198, 418, 827
SfcI CTRYAG 1 cut(s) 733
Sfr274I CTCGAG 3 cut(s) 27, 184, 999
SgrAI CRCCGGYG 1 cut(s) 139
SinI GGWCC 2 cut(s) 128, 856
SlaI CTCGAG 3 cut(s) 27, 184, 999
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 3 cut(s) 27, 184, 999
SmoI CTYRAG 3 cut(s) 27, 184, 999
Sse9I AATT 3 cut(s) 351, 709, 754
SsiI CCGC 6 cut(s) 143, 155, 200, 662, 796, 1089
SspI AATATT 1 cut(s) 727
SspMI CTAG 1 cut(s) 689
SstI GAGCTC 2 cut(s) 45, 387
StyD4I CCNGG 2 cut(s) 1048, 1077
TaaI ACNGT 5 cut(s) 128, 181, 342, 886, 1060
TaiI ACGT 2 cut(s) 780, 1033
TaqI TCGA 5 cut(s) 28, 171, 185, 192, 1000
TaqII GACCGA 1 cut(s) 868
TasI AATT 3 cut(s) 351, 709, 754
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 2 cut(s) 158, 202
TfiI GAWTC 2 cut(s) 629, 788
Tru1I TTAA 3 cut(s) 21, 360, 971
Tru9I TTAA 3 cut(s) 21, 360, 971
TscAI CASTG 5 cut(s) 409, 805, 891, 925, 1063
TseFI GTSAC 4 cut(s) 145, 310, 880, 1052
TseI GCWGC 2 cut(s) 60, 77
Tsp45I GTSAC 4 cut(s) 145, 310, 880, 1052
TspDTI ATGAA 6 cut(s) 347, 568, 599, 642, 767, 1113
TspGWI ACGGA 4 cut(s) 164, 205, 754, 1028
TspRI CASTG 5 cut(s) 409, 805, 891, 925, 1063
Van91I CCANNNNNTGG 1 cut(s) 1141
VpaK11BI GGWCC 2 cut(s) 128, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XhoI CTCGAG 3 cut(s) 27, 184, 999
XmiI GTMKAC 2 cut(s) 258, 1131
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 1 cut(s) 689
Zsp2I ATGCAT 2 cut(s) 433, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.