Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005497

Physical Location & Seq

Genomic Coordinates Reverse (-)

6496333 .. 6500374

Gene Structure 4042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1056600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGGACAAATCTAATTCCTTCAAAGCCTCTCAGAATCCATGGAGCGCCGATTTCGGACTCGGCGCCACCGATCGGAGATTGGCATTTTCCCGCCAAGCCTCGTTCCAGCAGTACCGCGAACCTCCGCACACGCCGGTCTCCATCAATCTCAACGACTCCGAGGCGACGCCGTTTCTCTTCCGGAGCGTGTCCAACATCGACGTTCCGCCGGGGGCGTATTTGACGGAGGAAAGCGACAAGATTTTCGGGGGCCGGAGCGTTTCTGCCGAGAAGTTATCGGTTTTGGTGGTTTTTGAATCGGTGTTTCGGATATTGAGGTCCGGAAACCGGTATATGAAGAGGTTGTTCTTGTTGATTTCGCTTAATGTGGCGTATTCGACAGTGGAGTTATTTATTGGACTCTTCACTGGACGTATAGGTTTGGTGTCGGATTCTTTTCATTTAAGTTTTGGTTGTGGCTTATTAACGTTTTCGTTATTCGCTATGGCGGCTTCTCGAAAGAAACCGGATGAAATTTACACTTATGGGTATAAAAGGCTAGAAGTTCTGTCTGCTTTTACCAATGCTCTGTTTCTTTTGTTTATGTCATTCTCCTTGGCCGTCGAAGCACTTCATGCTTTCATACAGGATGAATCTGAACACAAGCATTACTTGATTGTTTCGGCTGTGACAAATCTACTGGTGAACCTTATTGGTGTTTGGTTCTTCAGAAATTATGCTCGTGTAAATCTTGTTTACAGAAATGCAGAAGATATGAACAATCACTCTGTTTGCCTCCATGTTCTTGCAGATTCAATTCGCAGTGCAGGGTTGATATTGGCATCCTGGTTTCTGTCAATTGGGGTTCAGAATGCAGAGGTTTTGTGTTTTGGATTAGTTTCAGTTGCAGTTTTTATGCTCGTAATGCCACTATTTAGAGCGAGTGGTGGTATTCTACTCCAAATGGCACCACCGAATGTTCCACCTTCAGCATTGAGCAAATGCTGGCGACAGGTGGTTACTGCCCGCGAAGATGTTTCTGAAGTTTCACAAGCACGGTTTTGGGAATTGGTTCCTGGTCACGTTGTTGGGTCGCTCTCAGTACAGGTGAAGAAGGGGATAGATGATCGCCCCATTCTTCGATGTGTTCACGGTTTGTACCATGATTTGGGAATACAGGACTTGACAGTGCAAGCTGACAGTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009624 GO:0010035 GO:0010038 GO:0010043 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043207 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

396

Amino Acids

43.95

Weight (kDa)

7.71

Isoelectric Point (pI)

40.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	115 - 313	1.2e-31	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005497)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12100 AT3G12100
fragaria_vesca	FvH4_6g22170
malus_domestica	MD12G1056600.v1.1 MD14G1055100.v1.1
prunus_persica	Prupe.7G077600_v2.0.a1 Prupe.7G077600_v2.0.a1
pyrus_communis	pycom12g05070 pycom14g04540
rosa_chinensis	RchiOBHm_Chr2g0145321 RchiOBHm_Chr3g0479521
rosa_laevigata	RLG00000023562
rosa_multiflora	Rmu_sc0001786.1_g000012 Rmu_sc0004732.1_g000002 Rmu_sc0016947.1_g000001
rosa_roxburghii	Rroxscaffold_6G00402170
rosa_rugosa	Rorug03G0176200
rosa_samantha	Rh3AG227000 Rh3CG257000 Rh3DG252600 Rh3DG352500 Rh4AG055300 Rh4BG053300 Rh5BG537800 Rh5CG561700 Rh7DG359200
rosa_wichuraiana	Rw3G020280 Rw4G012830 Rw6G021660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	62, 946
AccB7I	CCANNNNNTGG	1 cut(s)	1147
AccII	CGCG	2 cut(s)	117, 1008
AccIII	TCCGGA	2 cut(s)	180, 320
AciI	CCGC	6 cut(s)	91, 115, 125, 206, 488, 1006
AclI	AACGTT	1 cut(s)	467
AcoI	YGGCCR	1 cut(s)	597
AcsI	RAATTY	1 cut(s)	513
AcuI	CTGAAG	3 cut(s)	691, 951, 1041
AcyI	GRCGYC	2 cut(s)	63, 167
AfaI	GTAC	3 cut(s)	113, 1083, 1139
AfiI	CCNNNNNNNGG	3 cut(s)	72, 327, 1147
AgeI	ACCGGT	1 cut(s)	327
AgsI	TTSAA	3 cut(s)	22, 296, 795
AjnI	CCWGG	2 cut(s)	824, 1054
AluBI	AGCT	1 cut(s)	1175
AluI	AGCT	1 cut(s)	1175
Alw26I	GTCTC	1 cut(s)	142
AlwNI	CAGNNNCTG	1 cut(s)	1185
Aor13HI	TCCGGA	2 cut(s)	180, 320
AoxI	GGCC	2 cut(s)	250, 597
ApoI	RAATTY	1 cut(s)	513
ArsI	GACNNNNNNTTYG	2 cut(s)	227, 259
AsiGI	ACCGGT	1 cut(s)	327
Asp700I	GAANNNNTTC	2 cut(s)	609, 1050
AspLEI	GCGC	2 cut(s)	47, 65
AspS9I	GGNCC	2 cut(s)	250, 318
AsuC2I	CCSGG	1 cut(s)	210
AsuHPI	GGTGA	2 cut(s)	694, 1099
AvaII	GGWCC	1 cut(s)	318
BanI	GGYRCC	2 cut(s)	62, 946
BauI	CACGAG	1 cut(s)	720
BccI	CCATC	1 cut(s)	149
BceAI	ACGGC	2 cut(s)	154, 584
BciT130I	CCWGG	2 cut(s)	826, 1056
BcnI	CCSGG	1 cut(s)	210
BcoDI	GTCTC	1 cut(s)	142
BfaI	CTAG	1 cut(s)	539
BfoI	RGCGCY	2 cut(s)	48, 66
BisI	GCNGC	1 cut(s)	489
BlsI	GCNGC	1 cut(s)	490
Bme1390I	CCNGG	3 cut(s)	210, 826, 1056
Bme18I	GGWCC	1 cut(s)	318
BmgT120I	GGNCC	2 cut(s)	250, 318
BmiI	GGNNCC	4 cut(s)	64, 251, 948, 1053
BmrFI	CCNGG	3 cut(s)	210, 826, 1056
BmsI	GCATC	1 cut(s)	830
BpuMI	CCSGG	1 cut(s)	210
BsaHI	GRCGYC	2 cut(s)	63, 167
BsaI	GGTCTC	1 cut(s)	142
BsaJI	CCNNGG	4 cut(s)	38, 159, 209, 594
BsaWI	WCCGGW	4 cut(s)	180, 320, 327, 505
Bsc4I	CCNNNNNNNGG	3 cut(s)	72, 327, 1147
Bse118I	RCCGGY	2 cut(s)	133, 327
Bse1I	ACTGG	2 cut(s)	412, 684
BseAI	TCCGGA	2 cut(s)	180, 320
BseBI	CCWGG	2 cut(s)	826, 1056
BseDI	CCNNGG	4 cut(s)	38, 159, 209, 594
BseGI	GGATG	3 cut(s)	514, 634, 821
BseLI	CCNNNNNNNGG	3 cut(s)	72, 327, 1147
BseMII	CTCAG	2 cut(s)	44, 1092
BseNI	ACTGG	2 cut(s)	412, 684
BsgI	GTGCAG	1 cut(s)	825
Bsh1236I	CGCG	2 cut(s)	117, 1008
Bsh1285I	CGRYCG	1 cut(s)	73
BshFI	GGCC	2 cut(s)	252, 599
BshNI	GGYRCC	2 cut(s)	62, 946
BshTI	ACCGGT	1 cut(s)	327
BsiEI	CGRYCG	1 cut(s)	73
BsiSI	CCGG	7 cut(s)	134, 181, 209, 253, 321, 328, 506
BslI	CCNNNNNNNGG	3 cut(s)	72, 327, 1147
BsmAI	GTCTC	1 cut(s)	142
BsmI	GAATGC	1 cut(s)	856
BsnI	GGCC	2 cut(s)	252, 599
Bso31I	GGTCTC	1 cut(s)	142
Bsp13I	TCCGGA	2 cut(s)	180, 320
Bsp143I	GATC	2 cut(s)	70, 1105
Bsp19I	CCATGG	1 cut(s)	38
BspACI	CCGC	6 cut(s)	91, 115, 125, 206, 488, 1006
BspANI	GGCC	2 cut(s)	252, 599
BspCNI	CTCAG	2 cut(s)	43, 1091
BspEI	TCCGGA	2 cut(s)	180, 320
BspFNI	CGCG	2 cut(s)	117, 1008
BspLI	GGNNCC	4 cut(s)	64, 251, 948, 1053
BspT107I	GGYRCC	2 cut(s)	62, 946
BspTNI	GGTCTC	1 cut(s)	142
BsrFI	RCCGGY	2 cut(s)	133, 327
BsrI	ACTGG	2 cut(s)	412, 684
BssAI	RCCGGY	2 cut(s)	133, 327
BssECI	CCNNGG	4 cut(s)	38, 159, 209, 594
BssMI	GATC	2 cut(s)	70, 1105
BssNI	GRCGYC	2 cut(s)	63, 167
BssSI	CACGAG	1 cut(s)	720
BssT1I	CCWWGG	2 cut(s)	38, 594
Bst2BI	CACGAG	1 cut(s)	720
Bst2UI	CCWGG	2 cut(s)	826, 1056
Bst4CI	ACNGT	5 cut(s)	382, 1038, 1133, 1168, 1181
Bst6I	CTCTTC	3 cut(s)	182, 332, 407
BstACI	GRCGYC	2 cut(s)	63, 167
BstAPI	GCANNNNNTGC	1 cut(s)	614
BstC8I	GCNNGC	3 cut(s)	986, 1006, 1173
BstDEI	CTNAG	2 cut(s)	30, 1078
BstDSI	CCRYGG	1 cut(s)	38
BstF5I	GGATG	3 cut(s)	514, 634, 821
BstFNI	CGCG	2 cut(s)	117, 1008
BstH2I	RGCGCY	2 cut(s)	48, 66
BstHHI	GCGC	2 cut(s)	47, 65
BstKTI	GATC	2 cut(s)	73, 1108
BstMAI	GTCTC	1 cut(s)	142
BstMBI	GATC	2 cut(s)	70, 1105
BstMCI	CGRYCG	1 cut(s)	73
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 605, 614, 904
BstNI	CCWGG	2 cut(s)	826, 1056
BstSCI	CCNGG	3 cut(s)	208, 824, 1054
BstUI	CGCG	2 cut(s)	117, 1008
BsuRI	GGCC	2 cut(s)	252, 599
BtgI	CCRYGG	1 cut(s)	38
BtsCI	GGATG	3 cut(s)	514, 634, 821
BtsI	GCAGTG	1 cut(s)	808
BtsIMutI	CAGTG	5 cut(s)	387, 405, 808, 1173, 1186
Cac8I	GCNNGC	3 cut(s)	986, 1006, 1173
CaiI	CAGNNNCTG	1 cut(s)	1185
CfoI	GCGC	2 cut(s)	47, 65
Cfr10I	RCCGGY	2 cut(s)	133, 327
Cfr13I	GGNCC	2 cut(s)	250, 318
CseI	GACGC	1 cut(s)	175
Csp6I	GTAC	3 cut(s)	112, 1082, 1138
CspAI	ACCGGT	1 cut(s)	327
CviAII	CATG	4 cut(s)	39, 614, 779, 1142
CviJI	RGCY	9 cut(s)	26, 98, 252, 459, 491, 538, 599, 665, 1175
CviKI_1	RGCY	9 cut(s)	26, 98, 252, 459, 491, 538, 599, 665, 1175
CviQI	GTAC	3 cut(s)	112, 1082, 1138
DdeI	CTNAG	2 cut(s)	30, 1078
DinI	GGCGCC	1 cut(s)	64
DpnI	GATC	2 cut(s)	72, 1107
DpnII	GATC	2 cut(s)	70, 1105
EaeI	YGGCCR	1 cut(s)	597
Eam1104I	CTCTTC	3 cut(s)	182, 332, 407
EarI	CTCTTC	3 cut(s)	182, 332, 407
EciI	GGCGGA	1 cut(s)	195
Eco130I	CCWWGG	2 cut(s)	38, 594
Eco31I	GGTCTC	1 cut(s)	142
Eco47I	GGWCC	1 cut(s)	318
Eco57I	CTGAAG	3 cut(s)	691, 951, 1041
EcoRII	CCWGG	2 cut(s)	824, 1054
EcoT14I	CCWWGG	2 cut(s)	38, 594
EgeI	GGCGCC	1 cut(s)	64
EheI	GGCGCC	1 cut(s)	64
ErhI	CCWWGG	2 cut(s)	38, 594
FaeI	CATG	4 cut(s)	42, 617, 782, 1145
FalI	AAGNNNNNCTT	2 cut(s)	635, 667
FatI	CATG	4 cut(s)	38, 613, 778, 1141
FauI	CCCGC	2 cut(s)	98, 1013
Fnu4HI	GCNGC	1 cut(s)	489
FokI	GGATG	3 cut(s)	521, 641, 808
Fsp4HI	GCNGC	1 cut(s)	489
FspBI	CTAG	1 cut(s)	539
GlaI	GCGC	2 cut(s)	46, 64
GluI	GCNGC	1 cut(s)	489
HaeII	RGCGCY	2 cut(s)	48, 66
HaeIII	GGCC	2 cut(s)	252, 599
HapII	CCGG	7 cut(s)	134, 181, 209, 253, 321, 328, 506
HgaI	GACGC	1 cut(s)	175
HhaI	GCGC	2 cut(s)	47, 65
Hin1I	GRCGYC	2 cut(s)	63, 167
Hin1II	CATG	4 cut(s)	42, 617, 782, 1145
Hin6I	GCGC	2 cut(s)	45, 63
HinP1I	GCGC	2 cut(s)	45, 63
HinfI	GANTC	8 cut(s)	34, 57, 155, 296, 399, 431, 632, 791
HpaII	CCGG	7 cut(s)	134, 181, 209, 253, 321, 328, 506
HphI	GGTGA	2 cut(s)	694, 1099
Hpy166II	GTNNAC	3 cut(s)	685, 736, 1129
Hpy188III	TCNNGA	3 cut(s)	181, 321, 495
Hpy8I	GTNNAC	3 cut(s)	685, 736, 1129
Hpy99I	CGWCG	3 cut(s)	169, 203, 605
HpyAV	CCTTC	3 cut(s)	28, 975, 1087
HpyCH4III	ACNGT	5 cut(s)	382, 1038, 1133, 1168, 1181
HpyCH4IV	ACGT	4 cut(s)	201, 412, 467, 1062
HpyCH4V	TGCA	6 cut(s)	746, 788, 806, 854, 887, 1171
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 605, 614, 904
HpyF3I	CTNAG	2 cut(s)	30, 1078
HpySE526I	ACGT	4 cut(s)	201, 412, 467, 1062
Hsp92I	GRCGYC	2 cut(s)	63, 167
Hsp92II	CATG	4 cut(s)	42, 617, 782, 1145
HspAI	GCGC	2 cut(s)	45, 63
KasI	GGCGCC	1 cut(s)	62
Kpn2I	TCCGGA	2 cut(s)	180, 320
Kzo9I	GATC	2 cut(s)	70, 1105
LmnI	GCTCC	3 cut(s)	42, 183, 255
LweI	GCATC	1 cut(s)	830
MaeI	CTAG	1 cut(s)	539
MaeII	ACGT	4 cut(s)	201, 412, 467, 1062
MaeIII	GTNAC	3 cut(s)	667, 997, 1058
MalI	GATC	2 cut(s)	72, 1107
MboI	GATC	2 cut(s)	70, 1105
MboII	GAAGA	8 cut(s)	169, 349, 394, 697, 761, 1022, 1102, 1109
MfeI	CAATTG	1 cut(s)	837
MluCI	AATT	6 cut(s)	13, 513, 712, 795, 837, 1046
Mly113I	GGCGCC	1 cut(s)	63
MlyI	GAGTC	3 cut(s)	51, 149, 393
MmeI	TCCRAC	2 cut(s)	216, 408
MnlI	CCTC	9 cut(s)	37, 109, 132, 154, 220, 309, 333, 785, 850
MroI	TCCGGA	2 cut(s)	180, 320
MroXI	GAANNNNTTC	2 cut(s)	609, 1050
MseI	TTAA	3 cut(s)	363, 443, 464
MspI	CCGG	7 cut(s)	134, 181, 209, 253, 321, 328, 506
MspR9I	CCNGG	3 cut(s)	210, 826, 1056
MunI	CAATTG	1 cut(s)	837
Mva1269I	GAATGC	1 cut(s)	856
MvaI	CCWGG	2 cut(s)	826, 1056
MvnI	CGCG	2 cut(s)	117, 1008
MwoI	GCNNNNNNNGC	4 cut(s)	488, 605, 614, 904
NarI	GGCGCC	1 cut(s)	63
NciI	CCSGG	1 cut(s)	210
NcoI	CCATGG	1 cut(s)	38
NdeII	GATC	2 cut(s)	70, 1105
NlaIII	CATG	4 cut(s)	42, 617, 782, 1145
NlaIV	GGNNCC	4 cut(s)	64, 251, 948, 1053
NmeAIII	GCCGAG	2 cut(s)	39, 292
NmuCI	GTSAC	2 cut(s)	667, 1058
PcsI	WCGNNNNNNNCGW	1 cut(s)	66
PctI	GAATGC	1 cut(s)	856
PdmI	GAANNNNTTC	2 cut(s)	609, 1050
PfeI	GAWTC	5 cut(s)	34, 296, 431, 632, 791
PflFI	GACNNNGTC	1 cut(s)	1181
PflMI	CCANNNNNTGG	1 cut(s)	1147
PinAI	ACCGGT	1 cut(s)	327
PkrI	GCNGC	1 cut(s)	490
Ple19I	CGATCG	1 cut(s)	73
PleI	GAGTC	3 cut(s)	51, 149, 393
PluTI	GGCGCC	1 cut(s)	66
PpsI	GAGTC	3 cut(s)	51, 149, 393
Psp1406I	AACGTT	1 cut(s)	467
Psp6I	CCWGG	2 cut(s)	824, 1054
PspGI	CCWGG	2 cut(s)	824, 1054
PspN4I	GGNNCC	4 cut(s)	64, 251, 948, 1053
PspPI	GGNCC	2 cut(s)	250, 318
PstNI	CAGNNNCTG	1 cut(s)	1185
PsyI	GACNNNGTC	1 cut(s)	1181
PvuI	CGATCG	1 cut(s)	73
RsaI	GTAC	3 cut(s)	113, 1083, 1139
RsaNI	GTAC	3 cut(s)	112, 1082, 1138
SaqAI	TTAA	3 cut(s)	363, 443, 464
SatI	GCNGC	1 cut(s)	489
Sau3AI	GATC	2 cut(s)	70, 1105
Sau96I	GGNCC	2 cut(s)	250, 318
SchI	GAGTC	3 cut(s)	51, 149, 393
ScrFI	CCNGG	3 cut(s)	210, 826, 1056
SfaNI	GCATC	1 cut(s)	830
SfoI	GGCGCC	1 cut(s)	64
SinI	GGWCC	1 cut(s)	318
Sse9I	AATT	6 cut(s)	13, 513, 712, 795, 837, 1046
SsiI	CCGC	6 cut(s)	91, 115, 125, 206, 488, 1006
SspDI	GGCGCC	1 cut(s)	62
SspMI	CTAG	1 cut(s)	539
StyD4I	CCNGG	3 cut(s)	208, 824, 1054
StyI	CCWWGG	2 cut(s)	38, 594
TaaI	ACNGT	5 cut(s)	382, 1038, 1133, 1168, 1181
TaiI	ACGT	4 cut(s)	204, 415, 470, 1065
TaqI	TCGA	5 cut(s)	198, 377, 496, 603, 1120
TasI	AATT	6 cut(s)	13, 513, 712, 795, 837, 1046
TatI	WGTACW	1 cut(s)	1081
TauI	GCSGC	1 cut(s)	491
TfiI	GAWTC	5 cut(s)	34, 296, 431, 632, 791
Tru1I	TTAA	3 cut(s)	363, 443, 464
Tru9I	TTAA	3 cut(s)	363, 443, 464
TscAI	CASTG	5 cut(s)	387, 412, 808, 1173, 1186
TseFI	GTSAC	2 cut(s)	667, 1058
Tsp45I	GTSAC	2 cut(s)	667, 1058
TspDTI	ATGAA	7 cut(s)	350, 428, 525, 602, 610, 645, 770
TspGWI	ACGGA	1 cut(s)	239
TspRI	CASTG	5 cut(s)	387, 412, 808, 1173, 1186
Tth111I	GACNNNGTC	1 cut(s)	1181
Van91I	CCANNNNNTGG	1 cut(s)	1147
VpaK11BI	GGWCC	1 cut(s)	318
XapI	RAATTY	1 cut(s)	513
XmnI	GAANNNNTTC	2 cut(s)	609, 1050
XspI	CTAG	1 cut(s)	539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.