FvH4_6g22170

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005497
Physical Location & Seq
Reverse (-)
15852146 .. 15856005
3860 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22170.t1

Sequence Viewer

Length: 879 bp
ATGGAACAGAGCAACTCCTTCAAAAACCCTCAGACTCCATGGAGCTCCGACCGTCTCGGACTCGGCGCCACCGATCGGAGGCTCGCCTTTTCCCGCCAAGCCTCCATCAACCTCAACGACTCGTCGTCCGCCACCACGCCTTTCCTGTCCCGCAGCGTCTCCAGCATCGACGTCCCGCCCGGCGCGTACTCCGATGGAAACGACAAGCTCTTCGGCCAGGGAAGAGTTTCCGCCGAGAAATTGACCTTTCTGATTGTATTCGAATCGGTTTTTCGGATCCTCAGGTCCGGAAATCGGTATATGAAGCGATTGTTTCTCTTGATTTCGCTCAACGTGGCGTATTCCACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTAGGTTTGGTGTCAGATGCATTTCATTTGACTTTCGGGTGTGGGCTGTTGACCTTTTCGCTGTTTGCGATGGCGGCTTCTCGAAAGAAGCCTGATGGAATCTACACTTACGGGTACAAGAGGCTTGAAGTTTTGTCAGCTTTTACCAATGCTCTGTTTCTTTTGTTTATGTCCTTCTCCTTGGCTGTAGAAGCACTTCACGCATTCATACAGGATGAATCTGAACACAAGCATTACTTGATTGTTTCGGCAGTGACAAATCTACTGGTGAATCTTATTGGTGTTTGGTTCTTCAGAAATTATGCTCGTATCAATCTTGTTTACAGGAATGCCGAGGATATGAACAACCACTCAGTTTGTCTGCACGTTCTCGCAGATTCCATACGCAGGGTTGATACTGGCATCCTGGTTTCTGTCTCTTGGGGTTCAGAATGCAGAAGTTTTGTGTTTTGGACTAGTTGCAGTTGTAGTCTTTATGCTTGTAATGCCACTCTTTAG

Protein Analysis

293

Amino Acids

32.54

Weight (kDa)

8.84

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 104 - 258 1.6e-28 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 185
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 6 cut(s) 94, 129, 151, 176, 231, 455
AclWI GGATC 2 cut(s) 271, 284
AcoI YGGCCR 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 658
AcyI GRCGYC 2 cut(s) 66, 171
AfaI GTAC 2 cut(s) 188, 497
AfiI CCNNNNNNNGG 4 cut(s) 75, 78, 294, 768
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 2 cut(s) 22, 509
AhdI GACNNNNNGTC 1 cut(s) 124
AhlI ACTAGT 1 cut(s) 836
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 2 cut(s) 216, 786
AluBI AGCT 3 cut(s) 45, 208, 521
AluI AGCT 3 cut(s) 45, 208, 521
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 3 cut(s) 59, 163, 802
AlwI GGATC 2 cut(s) 271, 284
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 1 cut(s) 153
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
Asp700I GAANNNNTTC 2 cut(s) 226, 576
AspLEI GCGC 2 cut(s) 68, 185
AspS9I GGNCC 1 cut(s) 285
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 364, 661
AsuII TTCGAA 1 cut(s) 261
AvaII GGWCC 1 cut(s) 285
AxyI CCTNAGG 1 cut(s) 281
BamHI GGATCC 1 cut(s) 276
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 47
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 165
BccI CCATC 4 cut(s) 113, 188, 445, 470
BciT130I CCWGG 2 cut(s) 218, 788
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 3 cut(s) 59, 163, 802
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 69
BisI GCNGC 2 cut(s) 154, 456
BlsI GCNGC 2 cut(s) 155, 457
Bme1390I CCNGG 3 cut(s) 180, 218, 788
Bme18I GGWCC 1 cut(s) 285
BmeRI GACNNNNNGTC 1 cut(s) 124
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 2 cut(s) 67, 278
BmrFI CCNGG 3 cut(s) 180, 218, 788
BmsI GCATC 3 cut(s) 174, 388, 792
BpmI CTGGAG 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 180
BsaHI GRCGYC 2 cut(s) 66, 171
BsaJI CCNNGG 4 cut(s) 38, 217, 561, 714
BsaWI WCCGGW 2 cut(s) 287, 374
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 78, 294, 768
Bse1I ACTGG 2 cut(s) 651, 784
Bse21I CCTNAGG 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 2 cut(s) 218, 788
BseDI CCNNGG 4 cut(s) 38, 217, 561, 714
BseGI GGATG 2 cut(s) 601, 783
BseLI CCNNNNNNNGG 4 cut(s) 75, 78, 294, 768
BseMII CTCAG 3 cut(s) 44, 295, 747
BseNI ACTGG 2 cut(s) 651, 784
BseXI GCAGC 1 cut(s) 165
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 2 cut(s) 52, 76
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 2 cut(s) 52, 76
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 3 cut(s) 180, 288, 375
BslFI GGGAC 2 cut(s) 133, 158
BslI CCNNNNNNNGG 4 cut(s) 75, 78, 294, 768
BsmAI GTCTC 3 cut(s) 59, 163, 802
BsmBI CGTCTC 2 cut(s) 59, 163
BsmFI GGGAC 2 cut(s) 133, 158
BsmI GAATGC 3 cut(s) 584, 715, 818
BsnI GGCC 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 47
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 2 cut(s) 73, 276
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 6 cut(s) 94, 129, 151, 176, 231, 455
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 3 cut(s) 43, 294, 746
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 67, 278
BspPI GGATC 2 cut(s) 271, 284
BspQI GCTCTTC 1 cut(s) 215
BspT104I TTCGAA 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 65
BsrI ACTGG 2 cut(s) 651, 784
BssECI CCNNGG 4 cut(s) 38, 217, 561, 714
BssMI GATC 2 cut(s) 73, 276
BssNI GRCGYC 2 cut(s) 66, 171
BssT1I CCWWGG 2 cut(s) 38, 561
Bst2UI CCWGG 2 cut(s) 218, 788
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 3 cut(s) 215, 217, 374
BstACI GRCGYC 2 cut(s) 66, 171
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 30, 281, 733
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 601, 783
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 185
BstKTI GATC 2 cut(s) 76, 279
BstMAI GTCTC 3 cut(s) 59, 163, 802
BstMBI GATC 2 cut(s) 73, 276
BstMCI CGRYCG 2 cut(s) 52, 76
BstMWI GCNNNNNNNGC 5 cut(s) 162, 455, 572, 581, 866
BstNI CCWGG 2 cut(s) 218, 788
BstSCI CCNGG 3 cut(s) 178, 216, 786
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
Bsu36I CCTNAGG 1 cut(s) 281
BsuRI GGCC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 464
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 2 cut(s) 601, 783
BtsI GCAGTG 2 cut(s) 345, 639
BtsIMutI CAGTG 2 cut(s) 345, 639
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 2 cut(s) 68, 185
Cfr13I GGNCC 1 cut(s) 285
CseI GACGC 1 cut(s) 145
Csp6I GTAC 2 cut(s) 187, 496
CspCI CAANNNNNGTGG 2 cut(s) 719, 754
CviAII CATG 1 cut(s) 39
CviQI GTAC 2 cut(s) 187, 496
DdeI CTNAG 3 cut(s) 30, 281, 733
DinI GGCGCC 1 cut(s) 67
DpnI GATC 2 cut(s) 75, 278
DpnII GATC 2 cut(s) 73, 276
DriI GACNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 3 cut(s) 215, 217, 374
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 3 cut(s) 215, 217, 374
EciI GGCGGA 2 cut(s) 118, 220
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 38, 561
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 658
Eco81I CCTNAGG 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 216, 786
EcoT14I CCWWGG 2 cut(s) 38, 561
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 38, 561
Esp3I CGTCTC 2 cut(s) 59, 163
FaeI CATG 1 cut(s) 42
FaiI YATR 9 cut(s) 40, 300, 302, 551, 590, 684, 722, 764, 858
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 2 cut(s) 133, 158
FatI CATG 1 cut(s) 38
FauI CCCGC 3 cut(s) 101, 158, 183
Fnu4HI GCNGC 2 cut(s) 154, 456
FokI GGATG 2 cut(s) 608, 770
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 154, 456
FspBI CTAG 1 cut(s) 837
GlaI GCGC 2 cut(s) 67, 184
GluI GCNGC 2 cut(s) 154, 456
GsuI CTGGAG 1 cut(s) 145
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 216
HapII CCGG 3 cut(s) 180, 288, 375
HgaI GACGC 1 cut(s) 145
HhaI GCGC 2 cut(s) 68, 185
Hin1I GRCGYC 2 cut(s) 66, 171
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 2 cut(s) 66, 183
HinP1I GCGC 2 cut(s) 66, 183
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 9 cut(s) 34, 60, 119, 263, 366, 480, 599, 652, 758
HpaII CCGG 3 cut(s) 180, 288, 375
HphI GGTGA 2 cut(s) 364, 661
Hpy166II GTNNAC 2 cut(s) 432, 703
Hpy188III TCNNGA 3 cut(s) 288, 319, 462
Hpy8I GTNNAC 2 cut(s) 432, 703
Hpy99I CGWCG 2 cut(s) 127, 173
HpyAV CCTTC 2 cut(s) 28, 565
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 4 cut(s) 171, 333, 379, 747
HpyCH4V TGCA 4 cut(s) 401, 745, 816, 843
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 455, 572, 581, 866
HpyF3I CTNAG 3 cut(s) 30, 281, 733
HpySE526I ACGT 4 cut(s) 171, 333, 379, 747
Hsp92I GRCGYC 2 cut(s) 66, 171
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 2 cut(s) 66, 183
KasI GGCGCC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 2 cut(s) 73, 276
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 2 cut(s) 42, 50
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 3 cut(s) 174, 388, 792
MaeI CTAG 1 cut(s) 837
MaeII ACGT 4 cut(s) 171, 333, 379, 747
MaeIII GTNAC 1 cut(s) 634
MalI GATC 2 cut(s) 75, 278
MboI GATC 2 cut(s) 73, 276
MboII GAAGA 4 cut(s) 202, 234, 361, 664
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 2 cut(s) 239, 679
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 4 cut(s) 28, 54, 113, 360
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 7 cut(s) 39, 72, 112, 122, 290, 495, 709
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 287
MroXI GAANNNNTTC 2 cut(s) 226, 576
MspI CCGG 3 cut(s) 180, 288, 375
MspR9I CCNGG 3 cut(s) 180, 218, 788
Mva1269I GAATGC 3 cut(s) 584, 715, 818
MvaI CCWGG 2 cut(s) 218, 788
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 5 cut(s) 162, 455, 572, 581, 866
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 180
NcoI CCATGG 1 cut(s) 38
NdeII GATC 2 cut(s) 73, 276
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 2 cut(s) 67, 278
NmeAIII GCCGAG 4 cut(s) 42, 259, 376, 739
NmuCI GTSAC 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 403
NspV TTCGAA 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 446
PctI GAATGC 3 cut(s) 584, 715, 818
PdmI GAANNNNTTC 2 cut(s) 226, 576
PfeI GAWTC 5 cut(s) 263, 480, 599, 652, 758
PkrI GCNGC 2 cut(s) 155, 457
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 4 cut(s) 28, 54, 113, 360
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 4 cut(s) 28, 54, 113, 360
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 216, 786
PspGI CCWGG 2 cut(s) 216, 786
PspN4I GGNNCC 2 cut(s) 67, 278
PspPI GGNCC 1 cut(s) 285
PsuI RGATCY 1 cut(s) 276
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 2 cut(s) 188, 497
RsaNI GTAC 2 cut(s) 187, 496
SacI GAGCTC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 215
SatI GCNGC 2 cut(s) 154, 456
Sau3AI GATC 2 cut(s) 73, 276
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 4 cut(s) 28, 54, 113, 360
ScrFI CCNGG 3 cut(s) 180, 218, 788
SduI GDGCHC 1 cut(s) 47
SfaNI GCATC 3 cut(s) 174, 388, 792
SfcI CTRYAG 1 cut(s) 567
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 836
Sse9I AATT 2 cut(s) 239, 679
SsiI CCGC 6 cut(s) 94, 129, 151, 176, 231, 455
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 1 cut(s) 837
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 178, 216, 786
StyI CCWWGG 2 cut(s) 38, 561
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 4 cut(s) 174, 336, 382, 750
TaqI TCGA 3 cut(s) 168, 261, 463
TasI AATT 2 cut(s) 239, 679
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 5 cut(s) 263, 480, 599, 652, 758
TscAI CASTG 2 cut(s) 352, 639
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 5 cut(s) 317, 395, 577, 612, 737
TspRI CASTG 2 cut(s) 352, 639
VpaK11BI GGWCC 1 cut(s) 285
XmnI GAANNNNTTC 2 cut(s) 226, 576
XspI CTAG 1 cut(s) 837
ZraI GACGTC 1 cut(s) 172
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.