Rw4G012830

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005497
Physical Location & Seq
Forward (+)
30176790 .. 30184958
8169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012830.1

Sequence Viewer

Length: 909 bp
ATGAATCACATTGAAATCTCAAACCACCCAAACATCCAAAACGCTTTCTTAAAACCTTCGCTGCTTCTTCCTCCTCTCCTACACTCTCTCGACATATCGACTCTCTCGTTCCACCAATACCACGACCCCCCGCACACGCCGCGCTCCATCAATCTCAACGACTCGTCGACGCCGTTTCTGTCCCGGAGTGTCTCCAGTATCGACGTCCCGCCGGCGGCCGAAGAAAACGACAAGCTTCTTTTCGGCGAAGGAAGAGTTTCGGCCGAGAAATTAACCTTTTTGGCGATATTCGAATCGGTCTTTCGGACGCTGAGGTCCGGAAATCGGCATATGAAGAGACTGTTCCTCTTGATTTCGCTCAACGTGGCTTATTCTACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTAGGTTTGGTGTCAGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTTGACGTTTTCGCTCTTCGCAATGGCGGCTTCTCGAAAGAAGCCTGATGGAATTTACACTTATGGGCTTGAAGTTTTGTCAGCTTTTACCAATGCTCTGTTTCTTTTGTTTATGTCATTCTCCTTGGCTGTAGAAGCGCTTCACGCATTCATACAGGATGAATCTGAGCACAAGGGAAATGTTTGCTTCCATTTGTTATATGAGTGGTGGCTTATCATTGATTTGGCTAGAAGCAGTAATATTTGCACTAATGCTTTGTTCTTTGCTATTTGGTTTAATGTCAGTGGTCCCTGTACTTCAAAATTCAGACCAATAGGTCCTTGCTGTCTAAAAGTCGCGGTGAAATGTCTATTATGCCAAGCGAGATTTTGTTATAGATTTATTCATGTTTGTGATATCGTTATCAATGATCGATTCTATCATGATCAATCAATCAGGTCTTGTAACTGA

Protein Analysis

302

Amino Acids

34.02

Weight (kDa)

7.6

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 114 - 210 1.5e-15 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 313, 774
AatII GACGTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 167
AccII CGCG 2 cut(s) 142, 797
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 6 cut(s) 131, 140, 209, 215, 485, 797
AcoI YGGCCR 2 cut(s) 216, 261
AcsI RAATTY 2 cut(s) 510, 761
AcyI GRCGYC 2 cut(s) 170, 204
AfaI GTAC 1 cut(s) 754
AfeI AGCGCT 1 cut(s) 597
AfiI CCNNNNNNNGG 2 cut(s) 214, 324
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 3 cut(s) 14, 530, 759
AjiI CACGTC 1 cut(s) 410
AluBI AGCT 2 cut(s) 235, 542
AluI AGCT 2 cut(s) 235, 542
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 2 cut(s) 196, 331
Aor13HI TCCGGA 1 cut(s) 317
Aor51HI AGCGCT 1 cut(s) 597
AoxI GGCC 2 cut(s) 216, 261
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 2 cut(s) 510, 761
Asp700I GAANNNNTTC 2 cut(s) 256, 597
AspLEI GCGC 2 cut(s) 144, 598
AspS9I GGNCC 3 cut(s) 315, 746, 776
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 2 cut(s) 394, 811
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 3 cut(s) 315, 746, 776
BarI GAAGNNNNNNTAC 2 cut(s) 582, 614
Bbv12I GWGCWC 1 cut(s) 630
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 48
BccI CCATC 2 cut(s) 155, 500
BceAI ACGGC 1 cut(s) 157
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 196, 331
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 599
BisI GCNGC 4 cut(s) 62, 140, 216, 486
BlsI GCNGC 4 cut(s) 63, 141, 217, 487
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 3 cut(s) 315, 746, 776
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 3 cut(s) 315, 746, 776
BmiI GGNNCC 1 cut(s) 748
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 184
Bsa29I ATCGAT 1 cut(s) 871
BsaHI GRCGYC 2 cut(s) 170, 204
BsaJI CCNNGG 1 cut(s) 582
BsaWI WCCGGW 2 cut(s) 317, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 324
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 317
BseCI ATCGAT 1 cut(s) 871
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 2 cut(s) 33, 622
BseLI CCNNNNNNNGG 2 cut(s) 214, 324
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 2 cut(s) 302, 615
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 63
BseX3I CGGCCG 2 cut(s) 216, 261
BseXI GCAGC 1 cut(s) 48
Bsh1236I CGCG 2 cut(s) 142, 797
Bsh1285I CGRYCG 2 cut(s) 219, 264
BshFI GGCC 2 cut(s) 218, 263
BshVI ATCGAT 1 cut(s) 871
BsiEI CGRYCG 2 cut(s) 219, 264
BsiHKAI GWGCWC 1 cut(s) 630
BsiSI CCGG 4 cut(s) 184, 212, 318, 405
BslFI GGGAC 3 cut(s) 166, 191, 732
BslI CCNNNNNNNGG 2 cut(s) 214, 324
BsmAI GTCTC 2 cut(s) 196, 331
BsmFI GGGAC 3 cut(s) 166, 191, 732
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 2 cut(s) 218, 263
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 630
Bsp13I TCCGGA 1 cut(s) 317
Bsp143I GATC 2 cut(s) 868, 883
BspACI CCGC 6 cut(s) 131, 140, 209, 215, 485, 797
BspANI GGCC 2 cut(s) 218, 263
BspCNI CTCAG 2 cut(s) 303, 616
BspDI ATCGAT 1 cut(s) 871
BspEI TCCGGA 1 cut(s) 317
BspFNI CGCG 2 cut(s) 142, 797
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 1 cut(s) 748
BspQI GCTCTTC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 2 cut(s) 868, 883
BssNI GRCGYC 2 cut(s) 170, 204
BssT1I CCWWGG 1 cut(s) 582
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 4 cut(s) 247, 329, 404, 479
BstACI GRCGYC 2 cut(s) 170, 204
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 311, 624
BstF5I GGATG 2 cut(s) 33, 622
BstFNI CGCG 2 cut(s) 142, 797
BstH2I RGCGCY 1 cut(s) 599
BstHHI GCGC 2 cut(s) 144, 598
BstKTI GATC 2 cut(s) 871, 886
BstMAI GTCTC 2 cut(s) 196, 331
BstMBI GATC 2 cut(s) 868, 883
BstMCI CGRYCG 2 cut(s) 219, 264
BstMWI GCNNNNNNNGC 4 cut(s) 139, 485, 593, 602
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 2 cut(s) 142, 797
BstV1I GCAGC 1 cut(s) 48
BstZI CGGCCG 2 cut(s) 216, 261
Bsu15I ATCGAT 1 cut(s) 871
BsuRI GGCC 2 cut(s) 218, 263
BsuTUI ATCGAT 1 cut(s) 871
BtrI CACGTC 1 cut(s) 410
BtsCI GGATG 2 cut(s) 33, 622
BtsIMutI CAGTG 1 cut(s) 748
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 880
CfoI GCGC 2 cut(s) 144, 598
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 3 cut(s) 315, 746, 776
ClaI ATCGAT 1 cut(s) 871
CseI GACGC 2 cut(s) 178, 316
Csp6I GTAC 1 cut(s) 753
CviAII CATG 2 cut(s) 845, 881
CviQI GTAC 1 cut(s) 753
DdeI CTNAG 2 cut(s) 311, 624
DpnI GATC 2 cut(s) 870, 885
DpnII GATC 2 cut(s) 868, 883
DrdI GACNNNNNNGTC 2 cut(s) 313, 774
DseDI GACNNNNNNGTC 2 cut(s) 313, 774
EaeI YGGCCR 2 cut(s) 216, 261
EagI CGGCCG 2 cut(s) 216, 261
Eam1104I CTCTTC 4 cut(s) 247, 329, 404, 479
EarI CTCTTC 4 cut(s) 247, 329, 404, 479
EclXI CGGCCG 2 cut(s) 216, 261
Eco130I CCWWGG 1 cut(s) 582
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 3 cut(s) 315, 746, 776
Eco47III AGCGCT 1 cut(s) 597
Eco52I CGGCCG 2 cut(s) 216, 261
EcoO109I RGGNCCY 1 cut(s) 776
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 582
EcoT22I ATGCAT 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 2 cut(s) 848, 884
FaqI GGGAC 3 cut(s) 166, 191, 732
FatI CATG 2 cut(s) 844, 880
FauI CCCGC 2 cut(s) 138, 216
FauNDI CATATG 1 cut(s) 330
FbaI TGATCA 1 cut(s) 883
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 62, 140, 216, 486
FokI GGATG 2 cut(s) 20, 629
Fsp4HI GCNGC 4 cut(s) 62, 140, 216, 486
FspBI CTAG 1 cut(s) 687
GlaI GCGC 2 cut(s) 143, 597
GluI GCNGC 4 cut(s) 62, 140, 216, 486
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 599
HaeIII GGCC 2 cut(s) 218, 263
HapII CCGG 4 cut(s) 184, 212, 318, 405
HgaI GACGC 2 cut(s) 178, 316
HhaI GCGC 2 cut(s) 144, 598
Hin1I GRCGYC 2 cut(s) 170, 204
Hin1II CATG 2 cut(s) 848, 884
Hin6I GCGC 2 cut(s) 142, 596
HinP1I GCGC 2 cut(s) 142, 596
HincII GTYRAC 2 cut(s) 168, 462
HindII GTYRAC 2 cut(s) 168, 462
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 7 cut(s) 4, 100, 161, 293, 396, 620, 873
HpaII CCGG 4 cut(s) 184, 212, 318, 405
HphI GGTGA 2 cut(s) 394, 811
Hpy166II GTNNAC 2 cut(s) 168, 462
Hpy188I TCNGA 4 cut(s) 306, 427, 625, 767
Hpy188III TCNNGA 5 cut(s) 89, 318, 349, 492, 881
Hpy8I GTNNAC 2 cut(s) 168, 462
Hpy99I CGWCG 3 cut(s) 169, 172, 206
HpyAV CCTTC 2 cut(s) 66, 242
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 4 cut(s) 204, 363, 409, 464
HpyCH4V TGCA 2 cut(s) 431, 705
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 485, 593, 602
HpyF3I CTNAG 2 cut(s) 311, 624
HpySE526I ACGT 4 cut(s) 204, 363, 409, 464
Hsp92I GRCGYC 2 cut(s) 170, 204
Hsp92II CATG 2 cut(s) 848, 884
HspAI GCGC 2 cut(s) 142, 596
Kpn2I TCCGGA 1 cut(s) 317
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 2 cut(s) 868, 883
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 9 cut(s) 197, 208, 225, 331, 418, 516, 599, 763, 880
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 687
MaeII ACGT 4 cut(s) 204, 363, 409, 464
MaeIII GTNAC 1 cut(s) 902
MalI GATC 2 cut(s) 870, 885
MboI GATC 2 cut(s) 868, 883
MboII GAAGA 6 cut(s) 59, 233, 264, 346, 391, 466
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 3 cut(s) 269, 510, 761
MlyI GAGTC 3 cut(s) 94, 155, 390
MnlI CCTC 4 cut(s) 81, 84, 306, 356
Mph1103I ATGCAT 1 cut(s) 433
MreI CGCCGGCG 1 cut(s) 211
MroI TCCGGA 1 cut(s) 317
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 2 cut(s) 256, 597
MseI TTAA 4 cut(s) 50, 272, 440, 735
MslI CAYNNNNRTG 1 cut(s) 849
MspI CCGG 4 cut(s) 184, 212, 318, 405
MspR9I CCNGG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 605
MvnI CGCG 2 cut(s) 142, 797
MwoI GCNNNNNNNGC 4 cut(s) 139, 485, 593, 602
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 184
NdeI CATATG 1 cut(s) 330
NdeII GATC 2 cut(s) 868, 883
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 2 cut(s) 848, 884
NlaIV GGNNCC 1 cut(s) 748
NmeAIII GCCGAG 2 cut(s) 289, 406
NsiI ATGCAT 1 cut(s) 433
NspV TTCGAA 1 cut(s) 291
PagI TCATGA 1 cut(s) 880
PciSI GCTCTTC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 170
PctI GAATGC 1 cut(s) 605
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 256, 597
PfeI GAWTC 4 cut(s) 4, 293, 620, 873
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 4 cut(s) 63, 141, 217, 487
PleI GAGTC 3 cut(s) 94, 155, 390
PpsI GAGTC 3 cut(s) 94, 155, 390
PpuMI RGGWCCY 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 776
PspN4I GGNNCC 1 cut(s) 748
PspPI GGNCC 3 cut(s) 315, 746, 776
PspPPI RGGWCCY 1 cut(s) 776
RsaI GTAC 1 cut(s) 754
RsaNI GTAC 1 cut(s) 753
RseI CAYNNNNRTG 1 cut(s) 849
SalI GTCGAC 1 cut(s) 166
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 4 cut(s) 50, 272, 440, 735
SatI GCNGC 4 cut(s) 62, 140, 216, 486
Sau3AI GATC 2 cut(s) 868, 883
Sau96I GGNCC 3 cut(s) 315, 746, 776
SchI GAGTC 3 cut(s) 94, 155, 390
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 291
SgrAI CRCCGGYG 1 cut(s) 211
SgrDI CGTCGACG 1 cut(s) 166
SinI GGWCC 3 cut(s) 315, 746, 776
SmiMI CAYNNNNRTG 1 cut(s) 849
Sse9I AATT 3 cut(s) 269, 510, 761
SsiI CCGC 6 cut(s) 131, 140, 209, 215, 485, 797
SspI AATATT 1 cut(s) 700
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 4 cut(s) 207, 366, 412, 467
TaqI TCGA 7 cut(s) 90, 98, 167, 201, 291, 493, 871
TaqII GACCGA 1 cut(s) 286
TasI AATT 3 cut(s) 269, 510, 761
TatI WGTACW 1 cut(s) 752
TauI GCSGC 3 cut(s) 142, 218, 488
TfiI GAWTC 4 cut(s) 4, 293, 620, 873
Tru1I TTAA 4 cut(s) 50, 272, 440, 735
Tru9I TTAA 4 cut(s) 50, 272, 440, 735
TscAI CASTG 1 cut(s) 748
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 6 cut(s) 17, 347, 425, 598, 633, 833
TspRI CASTG 1 cut(s) 748
VpaK11BI GGWCC 3 cut(s) 315, 746, 776
XapI RAATTY 2 cut(s) 510, 761
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 2 cut(s) 256, 597
XspI CTAG 1 cut(s) 687
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.