Rh3DG352500

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005497
Physical Location & Seq
Forward (+)
42349141 .. 42349754
614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG352500.1

Sequence Viewer

Length: 524 bp
ATGAATCACATTAAAATCTCAAACCACCCAAACCTCCAAAACGCTTTCTTAAAACCTTCGCTGCTTCTTCCTCCTCCCCTACACTCTCTCGACATATCGACTCTCTCGTTCCACCAATACCACGACCCCCCGCACACGCCGCGCTCCATTAATCCCAACGACTCGTCGACGCCGTTTCTGTCCCGGAGCGTCTCCAGTATCGACGTCCCGCCGGCGGCCGAAGAAAATGACAAGCTTCTTTTCAGCGAAGGAAGAGTTTCGGCCGAGAAATTAACCTTTTTGGCGATATTCGAATCGGTCTTTCGGACGCTGAGGTCCGGAAATCGGCATATGAAGAGACTGTTCCTCTTGATTTTGCTCAACGTGGCTTATTCTACTGTCGAGTTGTGTATTGGACTCTTCACCGGACGTGTAGGTTTGGTGTCAGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTGGACGTTTTCGCTCTTCGCAATGGCGGCTTCTCGAAAGAAGCCTGATGGAATTTACACTTATGG

Protein Analysis

174

Amino Acids

19.46

Weight (kDa)

9.0

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 114 - 174 1.7e-07 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 313
AatII GACGTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 167
AccII CGCG 1 cut(s) 142
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 5 cut(s) 131, 140, 209, 215, 485
AcoI YGGCCR 2 cut(s) 216, 261
AcsI RAATTY 1 cut(s) 510
AcyI GRCGYC 2 cut(s) 170, 204
AfiI CCNNNNNNNGG 2 cut(s) 214, 324
AflIII ACRYGT 1 cut(s) 409
AjiI CACGTC 1 cut(s) 410
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
Alw26I GTCTC 2 cut(s) 196, 331
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 2 cut(s) 216, 261
ApeKI GCWGC 1 cut(s) 61
ApoI RAATTY 1 cut(s) 510
AseI ATTAAT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 256
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 394
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 48
BccI CCATC 1 cut(s) 500
BceAI ACGGC 1 cut(s) 157
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 196, 331
BisI GCNGC 4 cut(s) 62, 140, 216, 486
BlsI GCNGC 4 cut(s) 63, 141, 217, 487
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 315
BmgBI CACGTC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 184
BsaHI GRCGYC 2 cut(s) 170, 204
BsaWI WCCGGW 2 cut(s) 317, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 324
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 214, 324
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 63
BseX3I CGGCCG 2 cut(s) 216, 261
BseXI GCAGC 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 142
Bsh1285I CGRYCG 2 cut(s) 219, 264
BshFI GGCC 2 cut(s) 218, 263
BsiEI CGRYCG 2 cut(s) 219, 264
BsiSI CCGG 4 cut(s) 184, 212, 318, 405
BslFI GGGAC 2 cut(s) 166, 191
BslI CCNNNNNNNGG 2 cut(s) 214, 324
BsmAI GTCTC 2 cut(s) 196, 331
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 2 cut(s) 166, 191
BsnI GGCC 2 cut(s) 218, 263
Bsp119I TTCGAA 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 317
BspACI CCGC 5 cut(s) 131, 140, 209, 215, 485
BspANI GGCC 2 cut(s) 218, 263
BspCNI CTCAG 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 317
BspFNI CGCG 1 cut(s) 142
BspQI GCTCTTC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 211
BssNI GRCGYC 2 cut(s) 170, 204
Bst4CI ACNGT 2 cut(s) 342, 379
Bst6I CTCTTC 4 cut(s) 247, 329, 404, 479
BstACI GRCGYC 2 cut(s) 170, 204
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 311
BstFNI CGCG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 144
BstMAI GTCTC 2 cut(s) 196, 331
BstMCI CGRYCG 2 cut(s) 219, 264
BstMWI GCNNNNNNNGC 2 cut(s) 139, 485
BstSCI CCNGG 1 cut(s) 182
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 48
BstZI CGGCCG 2 cut(s) 216, 261
BsuRI GGCC 2 cut(s) 218, 263
BtrI CACGTC 1 cut(s) 410
Cac8I GCNNGC 1 cut(s) 213
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 3 cut(s) 178, 178, 316
CviJI RGCY 7 cut(s) 218, 235, 263, 368, 456, 488, 502
CviKI_1 RGCY 7 cut(s) 218, 235, 263, 368, 456, 488, 502
DdeI CTNAG 1 cut(s) 311
DrdI GACNNNNNNGTC 1 cut(s) 313
DseDI GACNNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 216, 261
EagI CGGCCG 2 cut(s) 216, 261
Eam1104I CTCTTC 4 cut(s) 247, 329, 404, 479
EarI CTCTTC 4 cut(s) 247, 329, 404, 479
EclXI CGGCCG 2 cut(s) 216, 261
Eco47I GGWCC 1 cut(s) 315
Eco52I CGGCCG 2 cut(s) 216, 261
EcoT22I ATGCAT 1 cut(s) 433
Esp3I CGTCTC 1 cut(s) 196
FaiI YATR 4 cut(s) 95, 330, 332, 522
FaqI GGGAC 2 cut(s) 166, 191
FauI CCCGC 2 cut(s) 138, 216
FauNDI CATATG 1 cut(s) 330
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 62, 140, 216, 486
Fsp4HI GCNGC 4 cut(s) 62, 140, 216, 486
GlaI GCGC 1 cut(s) 143
GluI GCNGC 4 cut(s) 62, 140, 216, 486
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 2 cut(s) 218, 263
HapII CCGG 4 cut(s) 184, 212, 318, 405
HgaI GACGC 3 cut(s) 178, 178, 316
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 2 cut(s) 170, 204
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 5 cut(s) 4, 100, 161, 293, 396
HpaII CCGG 4 cut(s) 184, 212, 318, 405
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 2 cut(s) 168, 462
Hpy188I TCNGA 2 cut(s) 306, 427
Hpy188III TCNNGA 4 cut(s) 89, 318, 349, 492
Hpy8I GTNNAC 2 cut(s) 168, 462
Hpy99I CGWCG 3 cut(s) 169, 172, 206
HpyAV CCTTC 2 cut(s) 66, 242
HpyCH4III ACNGT 2 cut(s) 342, 379
HpyCH4IV ACGT 4 cut(s) 204, 363, 409, 464
HpyCH4V TGCA 1 cut(s) 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 485
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 4 cut(s) 204, 363, 409, 464
Hsp92I GRCGYC 2 cut(s) 170, 204
HspAI GCGC 1 cut(s) 142
Kpn2I TCCGGA 1 cut(s) 317
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 2 cut(s) 149, 186
LpnPI CCDG 6 cut(s) 197, 208, 225, 331, 418, 516
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 1 cut(s) 418
MaeII ACGT 4 cut(s) 204, 363, 409, 464
MboII GAAGA 6 cut(s) 59, 233, 264, 346, 391, 466
MluCI AATT 2 cut(s) 269, 510
MlyI GAGTC 3 cut(s) 94, 155, 390
MnlI CCTC 5 cut(s) 44, 81, 84, 306, 356
Mph1103I ATGCAT 1 cut(s) 433
MreI CGCCGGCG 1 cut(s) 211
MroI TCCGGA 1 cut(s) 317
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 5 cut(s) 12, 50, 150, 272, 440
MspI CCGG 4 cut(s) 184, 212, 318, 405
MspR9I CCNGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 139, 485
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 184
NdeI CATATG 1 cut(s) 330
NgoMIV GCCGGC 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 433
NspV TTCGAA 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 170
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 4, 293
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 4 cut(s) 63, 141, 217, 487
PleI GAGTC 3 cut(s) 94, 155, 390
PpsI GAGTC 3 cut(s) 94, 155, 390
PshBI ATTAAT 1 cut(s) 150
PspPI GGNCC 1 cut(s) 315
SalI GTCGAC 1 cut(s) 166
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 5 cut(s) 12, 50, 150, 272, 440
SatI GCNGC 4 cut(s) 62, 140, 216, 486
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 3 cut(s) 94, 155, 390
ScrFI CCNGG 1 cut(s) 184
SfaNI GCATC 1 cut(s) 418
SfuI TTCGAA 1 cut(s) 291
SgrAI CRCCGGYG 1 cut(s) 211
SgrDI CGTCGACG 1 cut(s) 166
SinI GGWCC 1 cut(s) 315
Sse9I AATT 2 cut(s) 269, 510
SsiI CCGC 5 cut(s) 131, 140, 209, 215, 485
StyD4I CCNGG 1 cut(s) 182
TaaI ACNGT 2 cut(s) 342, 379
TaiI ACGT 4 cut(s) 207, 366, 412, 467
TaqI TCGA 7 cut(s) 90, 98, 167, 201, 291, 381, 493
TaqII GACCGA 1 cut(s) 286
TasI AATT 2 cut(s) 269, 510
TauI GCSGC 3 cut(s) 142, 218, 488
TfiI GAWTC 2 cut(s) 4, 293
Tru1I TTAA 5 cut(s) 12, 50, 150, 272, 440
Tru9I TTAA 5 cut(s) 12, 50, 150, 272, 440
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 3 cut(s) 17, 347, 425
VpaK11BI GGWCC 1 cut(s) 315
VspI ATTAAT 1 cut(s) 150
XapI RAATTY 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 256
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.