Rh3CG257000

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005497
Physical Location & Seq
Reverse (-)
25074560 .. 25078605
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG257000.1

Sequence Viewer

Length: 1092 bp
ATGGAAAAGAGCAACTCCTTCAAAGGCTCCCAGAGTCCATGGAGCTCCGATTTCGGCCTGGGCGCCACCGATCGGAGGCTCGCGTTTTCCCGCCAAGCCTCGTTCCACCAATACCACGACCCCCCGCACACGCCGCGCTCCATCAATCTCAACGACTCGTCGACGCCGTTTCTGTCCCGGAGCGTCTCCAGTATCGACGTCCCGCCGGCGGCCGAAGAAAACGACAAGCTTCTTTTCGGCGAAGGAAGAGTTTCGGCCGAGAAATTAACCTTTCTGGCGATATTCGAATCGGTCTTTCGGACGCTGAGGTCCGGAAATCGGCATATGAAGAGACTGTTCCTCTTGATTTCGCTCAACGTGGCTTATTCTACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTAGGTTTGGTGTCAGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTTGACGTTTTCGCTCTTCGCAATGGCGGCTTCTCGAAAGAAGCCTGATGGAATTTACACTTATGGGTACAAGAGGCTTGAAGTTTTGTCAGCTTTTACCAATGCTCTGTTTCTGTTGTTTATGTCATTCTCCTTGGCTGTAGAAGCGCTTCACGCATTCATACAGGATGAATCTGAGCACAAGCATTACTTGATTGTTTCGGCAGTGACAAATCTACTGGTGAATCTTATTGGTGTTTGGTTCTTCAGAAATTATGCTCGTATCAATCTTGTTTACAGGAATGCCGAAGATATGAACAACCACTCAGTTTGTCTGCACGTTCTTGCAGATTCCATTCGCAGTGCAGGGTTGATACTGGCATCCTGGTTTCTGTCTCTTGGGGTTCAGAATGCAGAAGTTTTGTGTTTTGGACTAGTTGCAGTTGCAGTTTTTATGCTTGTAATGCCACTCTTTAGAGCAACTGGTGGTATTCTGCTCCAAATGGCACCACCAAACGTTCCAACTTCAGCATTGAGCAAATGCTGGCGACAGGTTGTATATGATTATATAAAAAGCTCTATTAGTAAATGGATTTTTCCAATATTTAGCATTACTTATTCCATATTCTTCTTAGATTACTGCCCGCGAAGATGTTTCTGA

Protein Analysis

363

Amino Acids

40.74

Weight (kDa)

9.04

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 112 - 310 3.2e-31 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AatII GACGTC 1 cut(s) 201
AccB1I GGYRCC 2 cut(s) 62, 937
AccI GTMKAC 1 cut(s) 161
AccII CGCG 3 cut(s) 83, 136, 1078
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 7 cut(s) 91, 125, 134, 203, 209, 479, 1076
AclI AACGTT 1 cut(s) 948
AcoI YGGCCR 2 cut(s) 210, 255
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 682, 942
AcyI GRCGYC 3 cut(s) 63, 164, 198
AfaI GTAC 1 cut(s) 521
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 4 cut(s) 72, 75, 208, 318
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 2 cut(s) 22, 533
AhlI ACTAGT 1 cut(s) 865
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 2 cut(s) 57, 815
AluBI AGCT 4 cut(s) 45, 229, 545, 1008
AluI AGCT 4 cut(s) 45, 229, 545, 1008
Alw21I GWGCWC 2 cut(s) 47, 633
Alw26I GTCTC 3 cut(s) 190, 325, 831
Aor13HI TCCGGA 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 3 cut(s) 55, 210, 255
ApoI RAATTY 1 cut(s) 504
Asp700I GAANNNNTTC 2 cut(s) 250, 600
AspLEI GCGC 3 cut(s) 65, 138, 601
AspS9I GGNCC 1 cut(s) 309
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 2 cut(s) 388, 685
AsuII TTCGAA 1 cut(s) 285
AvaII GGWCC 1 cut(s) 309
BanI GGYRCC 2 cut(s) 62, 937
BanII GRGCYC 1 cut(s) 47
BarI GAAGNNNNNNTAC 2 cut(s) 585, 617
Bbv12I GWGCWC 2 cut(s) 47, 633
BbvCI CCTCAGC 1 cut(s) 305
BccI CCATC 2 cut(s) 149, 494
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 59, 817
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 3 cut(s) 190, 325, 831
BcuI ACTAGT 1 cut(s) 865
BfaI CTAG 1 cut(s) 866
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 2 cut(s) 66, 602
BisI GCNGC 3 cut(s) 134, 210, 480
BlsI GCNGC 3 cut(s) 135, 211, 481
Bme1390I CCNGG 3 cut(s) 59, 178, 817
Bme18I GGWCC 1 cut(s) 309
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 3 cut(s) 28, 64, 939
BmrFI CCNGG 3 cut(s) 59, 178, 817
BmsI GCATC 2 cut(s) 412, 821
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 3 cut(s) 63, 164, 198
BsaJI CCNNGG 3 cut(s) 38, 58, 585
BsaWI WCCGGW 2 cut(s) 311, 398
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 75, 208, 318
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 4 cut(s) 189, 675, 813, 919
Bse3DI GCAATG 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 311
BseBI CCWGG 2 cut(s) 59, 817
BseDI CCNNGG 3 cut(s) 38, 58, 585
BseGI GGATG 2 cut(s) 625, 812
BseLI CCNNNNNNNGG 4 cut(s) 72, 75, 208, 318
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 3 cut(s) 296, 618, 771
BseNI ACTGG 4 cut(s) 189, 675, 813, 919
BseX3I CGGCCG 2 cut(s) 210, 255
BsgI GTGCAG 2 cut(s) 752, 816
Bsh1236I CGCG 3 cut(s) 83, 136, 1078
Bsh1285I CGRYCG 3 cut(s) 73, 213, 258
BshFI GGCC 3 cut(s) 57, 212, 257
BshNI GGYRCC 2 cut(s) 62, 937
BsiEI CGRYCG 3 cut(s) 73, 213, 258
BsiHKAI GWGCWC 2 cut(s) 47, 633
BsiSI CCGG 4 cut(s) 178, 206, 312, 399
BslFI GGGAC 2 cut(s) 160, 185
BslI CCNNNNNNNGG 4 cut(s) 72, 75, 208, 318
BsmAI GTCTC 3 cut(s) 190, 325, 831
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 160, 185
BsmI GAATGC 3 cut(s) 608, 739, 847
BsnI GGCC 3 cut(s) 57, 212, 257
Bsp119I TTCGAA 1 cut(s) 285
Bsp1286I GDGCHC 2 cut(s) 47, 633
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 70
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 7 cut(s) 91, 125, 134, 203, 209, 479, 1076
BspANI GGCC 3 cut(s) 57, 212, 257
BspCNI CTCAG 3 cut(s) 297, 619, 770
BspEI TCCGGA 1 cut(s) 311
BspFNI CGCG 3 cut(s) 83, 136, 1078
BspLI GGNNCC 3 cut(s) 28, 64, 939
BspQI GCTCTTC 1 cut(s) 473
BspT104I TTCGAA 1 cut(s) 285
BspT107I GGYRCC 2 cut(s) 62, 937
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 4 cut(s) 189, 675, 813, 919
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 38, 58, 585
BssMI GATC 1 cut(s) 70
BssNI GRCGYC 3 cut(s) 63, 164, 198
BssT1I CCWWGG 2 cut(s) 38, 585
Bst2UI CCWGG 2 cut(s) 59, 817
Bst4CI ACNGT 1 cut(s) 336
Bst6I CTCTTC 4 cut(s) 241, 323, 398, 473
BstACI GRCGYC 3 cut(s) 63, 164, 198
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 4 cut(s) 81, 207, 977, 1076
BstDEI CTNAG 4 cut(s) 305, 627, 757, 1063
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 625, 812
BstFNI CGCG 3 cut(s) 83, 136, 1078
BstH2I RGCGCY 2 cut(s) 66, 602
BstHHI GCGC 3 cut(s) 65, 138, 601
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 3 cut(s) 190, 325, 831
BstMBI GATC 1 cut(s) 70
BstMCI CGRYCG 3 cut(s) 73, 213, 258
BstMWI GCNNNNNNNGC 5 cut(s) 133, 479, 596, 605, 895
BstNI CCWGG 2 cut(s) 59, 817
BstSCI CCNGG 3 cut(s) 57, 176, 815
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 3 cut(s) 83, 136, 1078
BstZI CGGCCG 2 cut(s) 210, 255
BsuRI GGCC 3 cut(s) 57, 212, 257
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 625, 812
BtsI GCAGTG 2 cut(s) 663, 799
BtsIMutI CAGTG 2 cut(s) 663, 799
Cac8I GCNNGC 4 cut(s) 81, 207, 977, 1076
CfoI GCGC 3 cut(s) 65, 138, 601
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 309
CseI GACGC 3 cut(s) 172, 172, 310
Csp6I GTAC 1 cut(s) 520
CspCI CAANNNNNGTGG 2 cut(s) 743, 778
CviAII CATG 1 cut(s) 39
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 4 cut(s) 305, 627, 757, 1063
DinI GGCGCC 1 cut(s) 64
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 210, 255
EagI CGGCCG 2 cut(s) 210, 255
Eam1104I CTCTTC 4 cut(s) 241, 323, 398, 473
EarI CTCTTC 4 cut(s) 241, 323, 398, 473
Ecl136II GAGCTC 1 cut(s) 45
EclXI CGGCCG 2 cut(s) 210, 255
Eco130I CCWWGG 2 cut(s) 38, 585
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 309
Eco47III AGCGCT 1 cut(s) 600
Eco52I CGGCCG 2 cut(s) 210, 255
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 2 cut(s) 682, 942
EcoICRI GAGCTC 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 57, 815
EcoT14I CCWWGG 2 cut(s) 38, 585
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 2 cut(s) 38, 585
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 1 cut(s) 42
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 2 cut(s) 160, 185
FatI CATG 1 cut(s) 38
FauI CCCGC 4 cut(s) 98, 132, 210, 1083
FauNDI CATATG 1 cut(s) 324
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 134, 210, 480
FokI GGATG 2 cut(s) 632, 799
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 134, 210, 480
FspBI CTAG 1 cut(s) 866
GlaI GCGC 3 cut(s) 64, 137, 600
GluI GCNGC 3 cut(s) 134, 210, 480
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 2 cut(s) 66, 602
HaeIII GGCC 3 cut(s) 57, 212, 257
HapII CCGG 4 cut(s) 178, 206, 312, 399
HgaI GACGC 3 cut(s) 172, 172, 310
HhaI GCGC 3 cut(s) 65, 138, 601
Hin1I GRCGYC 3 cut(s) 63, 164, 198
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 3 cut(s) 63, 136, 599
HinP1I GCGC 3 cut(s) 63, 136, 599
HincII GTYRAC 2 cut(s) 162, 456
HindII GTYRAC 2 cut(s) 162, 456
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 7 cut(s) 34, 155, 287, 390, 623, 676, 782
HpaII CCGG 4 cut(s) 178, 206, 312, 399
HphI GGTGA 2 cut(s) 388, 685
Hpy166II GTNNAC 3 cut(s) 162, 456, 727
Hpy188I TCNGA 8 cut(s) 49, 75, 300, 421, 628, 701, 840, 1091
Hpy188III TCNNGA 3 cut(s) 312, 343, 486
Hpy8I GTNNAC 3 cut(s) 162, 456, 727
Hpy99I CGWCG 3 cut(s) 163, 166, 200
HpyAV CCTTC 2 cut(s) 28, 236
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4IV ACGT 6 cut(s) 198, 357, 403, 458, 771, 948
HpyCH4V TGCA 7 cut(s) 425, 769, 779, 797, 845, 872, 878
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 479, 596, 605, 895
HpyF3I CTNAG 4 cut(s) 305, 627, 757, 1063
HpySE526I ACGT 6 cut(s) 198, 357, 403, 458, 771, 948
Hsp92I GRCGYC 3 cut(s) 63, 164, 198
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 3 cut(s) 63, 136, 599
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 311
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
Kzo9I GATC 1 cut(s) 70
LguI GCTCTTC 1 cut(s) 473
LmnI GCTCC 6 cut(s) 32, 42, 50, 143, 180, 933
LweI GCATC 2 cut(s) 412, 821
MaeI CTAG 1 cut(s) 866
MaeII ACGT 6 cut(s) 198, 357, 403, 458, 771, 948
MaeIII GTNAC 1 cut(s) 658
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 9 cut(s) 227, 258, 340, 385, 460, 688, 752, 1051, 1092
MhlI GDGCHC 2 cut(s) 47, 633
MluCI AATT 3 cut(s) 263, 504, 703
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 43, 149, 384
MmeI TCCRAC 1 cut(s) 977
MnlI CCTC 5 cut(s) 69, 109, 300, 350, 519
Mph1103I ATGCAT 1 cut(s) 427
MreI CGCCGGCG 1 cut(s) 205
MroI TCCGGA 1 cut(s) 311
MroNI GCCGGC 1 cut(s) 205
MroXI GAANNNNTTC 2 cut(s) 250, 600
MseI TTAA 2 cut(s) 266, 434
MspI CCGG 4 cut(s) 178, 206, 312, 399
MspR9I CCNGG 3 cut(s) 59, 178, 817
Mva1269I GAATGC 3 cut(s) 608, 739, 847
MvaI CCWGG 2 cut(s) 59, 817
MvnI CGCG 3 cut(s) 83, 136, 1078
MwoI GCNNNNNNNGC 5 cut(s) 133, 479, 596, 605, 895
NaeI GCCGGC 1 cut(s) 207
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 178
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 1 cut(s) 324
NdeII GATC 1 cut(s) 70
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 3 cut(s) 28, 64, 939
NmeAIII GCCGAG 2 cut(s) 283, 400
NmuCI GTSAC 1 cut(s) 658
NsiI ATGCAT 1 cut(s) 427
NspV TTCGAA 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 3 cut(s) 608, 739, 847
PdiI GCCGGC 1 cut(s) 207
PdmI GAANNNNTTC 2 cut(s) 250, 600
PfeI GAWTC 4 cut(s) 287, 623, 676, 782
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 3 cut(s) 135, 211, 481
Ple19I CGATCG 1 cut(s) 73
PleI GAGTC 3 cut(s) 42, 149, 384
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 42, 149, 384
Psp124BI GAGCTC 1 cut(s) 47
Psp1406I AACGTT 1 cut(s) 948
Psp6I CCWGG 2 cut(s) 57, 815
PspGI CCWGG 2 cut(s) 57, 815
PspN4I GGNNCC 3 cut(s) 28, 64, 939
PspPI GGNCC 1 cut(s) 309
PvuI CGATCG 1 cut(s) 73
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SacI GAGCTC 1 cut(s) 47
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 473
SaqAI TTAA 2 cut(s) 266, 434
SatI GCNGC 3 cut(s) 134, 210, 480
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 3 cut(s) 43, 149, 384
ScrFI CCNGG 3 cut(s) 59, 178, 817
SduI GDGCHC 2 cut(s) 47, 633
SfaNI GCATC 2 cut(s) 412, 821
SfcI CTRYAG 1 cut(s) 591
SfoI GGCGCC 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 285
SgrAI CRCCGGYG 1 cut(s) 205
SgrDI CGTCGACG 1 cut(s) 160
SinI GGWCC 1 cut(s) 309
SpeI ACTAGT 1 cut(s) 865
Sse9I AATT 3 cut(s) 263, 504, 703
SsiI CCGC 7 cut(s) 91, 125, 134, 203, 209, 479, 1076
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 1035
SspMI CTAG 1 cut(s) 866
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 57, 176, 815
StyI CCWWGG 2 cut(s) 38, 585
TaaI ACNGT 1 cut(s) 336
TaiI ACGT 6 cut(s) 201, 360, 406, 461, 774, 951
TaqI TCGA 4 cut(s) 161, 195, 285, 487
TaqII GACCGA 1 cut(s) 280
TasI AATT 3 cut(s) 263, 504, 703
TauI GCSGC 3 cut(s) 136, 212, 482
TfiI GAWTC 4 cut(s) 287, 623, 676, 782
Tru1I TTAA 2 cut(s) 266, 434
Tru9I TTAA 2 cut(s) 266, 434
TscAI CASTG 2 cut(s) 663, 799
TseFI GTSAC 1 cut(s) 658
Tsp45I GTSAC 1 cut(s) 658
TspDTI ATGAA 5 cut(s) 341, 419, 601, 636, 761
TspRI CASTG 2 cut(s) 663, 799
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 1 cut(s) 504
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 2 cut(s) 250, 600
XspI CTAG 1 cut(s) 866
ZraI GACGTC 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.