Rh4AG055300

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005497
Physical Location & Seq
Forward (+)
11560399 .. 11580801
20403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG055300.1

Sequence Viewer

Length: 1167 bp
ATGAATCACATTGAAATCGCAAACCACCCAAACCTCCAAAACGCTTTCTTAAAACCTTCGCTGCTTCTTCCTCCTCCCCTACACTCTCTCGACATATCGACTCTCTCGTTCCACCAATACCACGACCCCCCACACACGCAGCGCTCCATCAATCTCAATGACTCGTCGACGCCGTTTCTGTCCCGGAGCGTCTCCAGTATCGACGTCCGGCCGGCGGCCGAAGAAAACGACAAGCTTCTTTTCGGCGAAGGAAGAGTTTCGGCCGAGAAATTAACCTTTTTGGCAATATTCGAATCGGTCTTTCGGGACGCTGAGGTCCGGAAATCGGCATATGAAGAGACTGTTCCTCTTGATTTCGCTCAACGTGGCTTATTCTACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTTTGGTGTCAGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTTGACGTTTTCGCTCTTCGCAATGACGGCTTCTCGAAAGAAGCCTGATGGAATTTACACTCATGGATTTCGGGATGTTTTGGACTTGAGAATCATGTATTCGGATGTGAAAATGGGAGCTGGAAGGAAGACTCAAACCTTTATTTTGCCTCCTAATGCAGACCTGGCACCCTACCCTGTACAAGCAAGACAATTGAGAATTTTGGTTTGGTTCCAGAACTCATGTGATGCTTACAAATTCGTTCAGGTTCAAATTCGCATCCGGCTGCAGTGCAAACCACTGCTTGAAAGTCAATTTAAACCAATTATTGCAGACAACTACTACAGTGCAAATAAATTCTGGTTTGAGATTGATCATGCTGAGACAAACAAGCTGATGTCTTTGTTAGCATCTTGCGCGGTTGTGCAATCTCTCCAGTTGGTTCAAGAGTTAGAAGAGCTAAAGGCTTTCAAGAATGAACAAACTAAGAAGATAGGCTATTTGGAGTATAAGCCGATAGGACTTTGCCCAGAAGCTGCAAATCACTTGCAAGAAATGAAGGACATGCTAATTGCCATATCAAATGAACTGCTTGATGATGTCAATGAACTCAGTCTAAAGCAAATAGAGAAGCTTCGCCAAGATAGACATATGTTTGATCTTAGTTGTGAGTTGTGCCACTGCGGAGTGAAGAGAAACAGGTCAGCAAACCTCCACAAGTATCACAAGTAG

Protein Analysis

388

Amino Acids

44.61

Weight (kDa)

7.62

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 233 - 280 1.1e-13 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 167
AccII CGCG 1 cut(s) 854
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 3 cut(s) 215, 854, 1119
AcoI YGGCCR 3 cut(s) 209, 216, 261
AcsI RAATTY 5 cut(s) 507, 654, 692, 708, 791
AcyI GRCGYC 2 cut(s) 170, 204
AfaI GTAC 1 cut(s) 636
AfeI AGCGCT 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 214, 325
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 5 cut(s) 14, 707, 743, 881, 907
AjiI CACGTC 1 cut(s) 411
AjnI CCWGG 1 cut(s) 618
AjuI GAANNNNNNNTTGG 2 cut(s) 646, 678
AluBI AGCT 6 cut(s) 235, 575, 829, 895, 971, 1069
AluI AGCT 6 cut(s) 235, 575, 829, 895, 971, 1069
Alw26I GTCTC 3 cut(s) 196, 332, 812
AlwNI CAGNNNCTG 1 cut(s) 971
Aor13HI TCCGGA 1 cut(s) 318
Aor51HI AGCGCT 1 cut(s) 143
AoxI GGCC 3 cut(s) 209, 216, 261
ApeKI GCWGC 4 cut(s) 61, 139, 721, 971
ApoI RAATTY 5 cut(s) 507, 654, 692, 708, 791
Asp700I GAANNNNTTC 1 cut(s) 256
AspLEI GCGC 2 cut(s) 144, 854
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 48, 151, 708, 958
BccI CCATC 2 cut(s) 155, 497
BceAI ACGGC 2 cut(s) 157, 498
BciT130I CCWGG 1 cut(s) 620
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 3 cut(s) 196, 332, 812
BfmI CTRYAG 2 cut(s) 722, 778
BfoI RGCGCY 1 cut(s) 145
BisI GCNGC 5 cut(s) 62, 140, 216, 722, 972
BlsI GCNGC 5 cut(s) 63, 141, 217, 723, 973
Bme1390I CCNGG 2 cut(s) 184, 620
Bme18I GGWCC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 624, 668
BmrFI CCNGG 2 cut(s) 184, 620
BmsI GCATC 4 cut(s) 415, 673, 723, 854
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 2 cut(s) 178, 854
Bpu10I CCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 562
BpuMI CCSGG 1 cut(s) 184
BsaHI GRCGYC 2 cut(s) 170, 204
BsaWI WCCGGW 2 cut(s) 318, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 325
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 2 cut(s) 195, 871
Bse3DI GCAATG 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 1 cut(s) 620
BseGI GGATG 3 cut(s) 535, 565, 714
BseLI CCNNNNNNNGG 2 cut(s) 214, 325
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 3 cut(s) 303, 807, 1060
BseNI ACTGG 2 cut(s) 195, 871
BseRI GAGGAG 1 cut(s) 63
BseX3I CGGCCG 3 cut(s) 209, 216, 261
BseXI GCAGC 4 cut(s) 48, 151, 708, 958
Bsh1236I CGCG 1 cut(s) 854
Bsh1285I CGRYCG 3 cut(s) 212, 219, 264
BshFI GGCC 3 cut(s) 211, 218, 263
BshNI GGYRCC 1 cut(s) 622
BsiEI CGRYCG 3 cut(s) 212, 219, 264
BsiSI CCGG 6 cut(s) 184, 208, 212, 319, 406, 718
BslFI GGGAC 2 cut(s) 166, 320
BslI CCNNNNNNNGG 2 cut(s) 214, 325
BsmAI GTCTC 3 cut(s) 196, 332, 812
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 2 cut(s) 166, 320
BsnI GGCC 3 cut(s) 211, 218, 263
Bsp119I TTCGAA 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 318
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 2 cut(s) 808, 1093
BspACI CCGC 3 cut(s) 215, 854, 1119
BspANI GGCC 3 cut(s) 211, 218, 263
BspCNI CTCAG 3 cut(s) 304, 808, 1059
BspEI TCCGGA 1 cut(s) 318
BspFNI CGCG 1 cut(s) 854
BspLI GGNNCC 2 cut(s) 624, 668
BspMAI CTGCAG 1 cut(s) 726
BspQI GCTCTTC 2 cut(s) 476, 885
BspT104I TTCGAA 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 2 cut(s) 195, 871
BssAI RCCGGY 1 cut(s) 211
BssMI GATC 2 cut(s) 808, 1093
BssNI GRCGYC 2 cut(s) 170, 204
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 2 cut(s) 343, 782
Bst6I CTCTTC 6 cut(s) 247, 330, 405, 476, 885, 1121
BstACI GRCGYC 2 cut(s) 170, 204
BstAUI TGTACA 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 5 cut(s) 312, 816, 921, 1046, 1097
BstF5I GGATG 3 cut(s) 535, 565, 714
BstFNI CGCG 1 cut(s) 854
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 2 cut(s) 144, 854
BstKTI GATC 2 cut(s) 811, 1096
BstMAI GTCTC 3 cut(s) 196, 332, 812
BstMBI GATC 2 cut(s) 808, 1093
BstMCI CGRYCG 3 cut(s) 212, 219, 264
BstMWI GCNNNNNNNGC 3 cut(s) 482, 620, 851
BstNI CCWGG 1 cut(s) 620
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 2 cut(s) 182, 618
BstSFI CTRYAG 2 cut(s) 722, 778
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 4 cut(s) 48, 151, 708, 958
BstV2I GAAGAC 1 cut(s) 590
BstZI CGGCCG 3 cut(s) 209, 216, 261
BsuRI GGCC 3 cut(s) 211, 218, 263
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 3 cut(s) 535, 565, 714
BtsI GCAGTG 3 cut(s) 731, 734, 1114
BtsIMutI CAGTG 4 cut(s) 731, 734, 787, 1114
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 971
CfoI GCGC 2 cut(s) 144, 854
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 3 cut(s) 178, 178, 317
Csp6I GTAC 1 cut(s) 635
CviAII CATG 5 cut(s) 518, 550, 678, 812, 1000
CviQI GTAC 1 cut(s) 635
DdeI CTNAG 5 cut(s) 312, 816, 921, 1046, 1097
DpnI GATC 2 cut(s) 810, 1095
DpnII GATC 2 cut(s) 808, 1093
DraI TTTAAA 1 cut(s) 754
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 3 cut(s) 209, 216, 261
EagI CGGCCG 3 cut(s) 209, 216, 261
Eam1104I CTCTTC 6 cut(s) 247, 330, 405, 476, 885, 1121
EarI CTCTTC 6 cut(s) 247, 330, 405, 476, 885, 1121
EclXI CGGCCG 3 cut(s) 209, 216, 261
Eco47I GGWCC 1 cut(s) 316
Eco47III AGCGCT 1 cut(s) 143
Eco52I CGGCCG 3 cut(s) 209, 216, 261
EcoRII CCWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 5 cut(s) 521, 553, 681, 815, 1003
FaqI GGGAC 2 cut(s) 166, 320
FatI CATG 5 cut(s) 517, 549, 677, 811, 999
FauNDI CATATG 2 cut(s) 331, 1086
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 5 cut(s) 62, 140, 216, 722, 972
FokI GGATG 3 cut(s) 542, 572, 701
Fsp4HI GCNGC 5 cut(s) 62, 140, 216, 722, 972
GlaI GCGC 2 cut(s) 143, 853
GluI GCNGC 5 cut(s) 62, 140, 216, 722, 972
GsuI CTGGAG 2 cut(s) 178, 854
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 3 cut(s) 211, 218, 263
HapII CCGG 6 cut(s) 184, 208, 212, 319, 406, 718
HgaI GACGC 3 cut(s) 178, 178, 317
HhaI GCGC 2 cut(s) 144, 854
Hin1I GRCGYC 2 cut(s) 170, 204
Hin1II CATG 5 cut(s) 521, 553, 681, 815, 1003
Hin6I GCGC 2 cut(s) 142, 852
HinP1I GCGC 2 cut(s) 142, 852
HincII GTYRAC 2 cut(s) 168, 459
HindII GTYRAC 2 cut(s) 168, 459
HindIII AAGCTT 2 cut(s) 233, 1067
HinfI GANTC 7 cut(s) 4, 100, 161, 293, 397, 546, 586
HpaII CCGG 6 cut(s) 184, 208, 212, 319, 406, 718
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 168, 459
Hpy188I TCNGA 2 cut(s) 424, 559
Hpy188III TCNNGA 9 cut(s) 89, 305, 319, 350, 489, 527, 670, 881, 907
Hpy8I GTNNAC 2 cut(s) 168, 459
Hpy99I CGWCG 3 cut(s) 169, 172, 206
HpyAV CCTTC 4 cut(s) 66, 242, 573, 988
HpyCH4III ACNGT 2 cut(s) 343, 782
HpyCH4IV ACGT 4 cut(s) 204, 364, 410, 461
HpyCH4V TGCA 9 cut(s) 428, 614, 724, 729, 767, 785, 862, 974, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 482, 620, 851
HpyF3I CTNAG 5 cut(s) 312, 816, 921, 1046, 1097
HpySE526I ACGT 4 cut(s) 204, 364, 410, 461
Hsp92I GRCGYC 2 cut(s) 170, 204
Hsp92II CATG 5 cut(s) 521, 553, 681, 815, 1003
HspAI GCGC 2 cut(s) 142, 852
Kpn2I TCCGGA 1 cut(s) 318
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 2 cut(s) 808, 1093
LguI GCTCTTC 2 cut(s) 476, 885
LmnI GCTCC 3 cut(s) 149, 186, 572
Lsp1109I GCAGC 4 cut(s) 48, 151, 708, 958
LweI GCATC 4 cut(s) 415, 673, 723, 854
MaeII ACGT 4 cut(s) 204, 364, 410, 461
MalI GATC 2 cut(s) 810, 1095
MboI GATC 2 cut(s) 808, 1093
MfeI CAATTG 1 cut(s) 647
MlyI GAGTC 4 cut(s) 94, 155, 391, 580
MnlI CCTC 7 cut(s) 44, 81, 84, 307, 357, 615, 1157
Mph1103I ATGCAT 1 cut(s) 430
MroI TCCGGA 1 cut(s) 318
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 4 cut(s) 50, 272, 437, 753
MspI CCGG 6 cut(s) 184, 208, 212, 319, 406, 718
MspR9I CCNGG 2 cut(s) 184, 620
MunI CAATTG 1 cut(s) 647
MvaI CCWGG 1 cut(s) 620
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 3 cut(s) 482, 620, 851
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 184
NdeI CATATG 2 cut(s) 331, 1086
NdeII GATC 2 cut(s) 808, 1093
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 5 cut(s) 521, 553, 681, 815, 1003
NlaIV GGNNCC 2 cut(s) 624, 668
NmeAIII GCCGAG 2 cut(s) 289, 407
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 1003
NspV TTCGAA 1 cut(s) 291
PciSI GCTCTTC 2 cut(s) 476, 885
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 170
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 3 cut(s) 4, 293, 546
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 5 cut(s) 63, 141, 217, 723, 973
PleI GAGTC 4 cut(s) 94, 155, 391, 580
PpsI GAGTC 4 cut(s) 94, 155, 391, 580
Psp6I CCWGG 1 cut(s) 618
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 2 cut(s) 624, 668
PspPI GGNCC 1 cut(s) 316
PstI CTGCAG 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 971
RsaI GTAC 1 cut(s) 636
RsaNI GTAC 1 cut(s) 635
SalI GTCGAC 1 cut(s) 166
SapI GCTCTTC 2 cut(s) 476, 885
SaqAI TTAA 4 cut(s) 50, 272, 437, 753
SatI GCNGC 5 cut(s) 62, 140, 216, 722, 972
Sau3AI GATC 2 cut(s) 808, 1093
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 4 cut(s) 94, 155, 391, 580
ScrFI CCNGG 2 cut(s) 184, 620
SfaNI GCATC 4 cut(s) 415, 673, 723, 854
SfcI CTRYAG 2 cut(s) 722, 778
SfuI TTCGAA 1 cut(s) 291
SgrDI CGTCGACG 1 cut(s) 166
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
SsiI CCGC 3 cut(s) 215, 854, 1119
SspI AATATT 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 182, 618
TaaI ACNGT 2 cut(s) 343, 782
TaiI ACGT 4 cut(s) 207, 367, 413, 464
TaqI TCGA 6 cut(s) 90, 98, 167, 201, 291, 490
TaqII GACCGA 1 cut(s) 286
TatI WGTACW 1 cut(s) 634
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 3 cut(s) 4, 293, 546
Tru1I TTAA 4 cut(s) 50, 272, 437, 753
Tru9I TTAA 4 cut(s) 50, 272, 437, 753
TscAI CASTG 4 cut(s) 731, 741, 787, 1121
TseI GCWGC 4 cut(s) 61, 139, 721, 971
TspDTI ATGAA 7 cut(s) 17, 348, 422, 927, 1007, 1035, 1056
TspRI CASTG 4 cut(s) 731, 741, 787, 1121
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 5 cut(s) 507, 654, 692, 708, 791
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 256
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.