Rh4BG053300

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005497
Physical Location & Seq
Forward (+)
9537134 .. 9538790
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG053300.1

Sequence Viewer

Length: 621 bp
ATGAATCACATTGAAATCGCAAACCACCCAAACCTCCAAAACGCTTTCTTAAAACCTTCGCTGCTTCTTCCTCCTCCCCTACACTCTCTCGACATATCGACTCTCTCGTTCCACCAATACCACGACCCCCCACACACGCAGCGCTCCATCAATCTCAATGACTCGTCGACGCCGTTTCTGTCCCGGAGCGTCTCCAGTATCGACGTCCGGCCGGCGGCCGAAGAAAACGACAAGCTTCTTTTCGGCGAAGGAAGAGTTTCGGCCGAGAAATTAACCTTTTTGGCAATATTCGAATCGGTCTTTCGGGACGCTGAGGTCCGGAAATCGGCATATGAAGAGACTGTTCCTCTTGATTTCGCTCAACGTGGCTTATTCTACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTTTGGTGTCAGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTTGACGTTTTCGCTCTTCGCAATGACGGCTTCTCGAAAGAAGCCTGATGGAATTTACACTCATGGGTACAAGAGGCTTGAAGTTTTGTCAGCTTTTACCAATGCTGTATTCGGATGTGAAAATGGGAGCTGGAAGGAAGACTCAAACCTTTATTTTGCCTCCTAA

Protein Analysis

206

Amino Acids

23.3

Weight (kDa)

6.29

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 129 - 189 2.8e-11 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 1 cut(s) 215
AcoI YGGCCR 3 cut(s) 209, 216, 261
AcsI RAATTY 1 cut(s) 507
AcyI GRCGYC 2 cut(s) 170, 204
AfaI GTAC 1 cut(s) 524
AfeI AGCGCT 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 214, 325
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 2 cut(s) 14, 536
AjiI CACGTC 1 cut(s) 411
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AluBI AGCT 3 cut(s) 235, 548, 585
AluI AGCT 3 cut(s) 235, 548, 585
Alw26I GTCTC 2 cut(s) 196, 332
Aor13HI TCCGGA 1 cut(s) 318
Aor51HI AGCGCT 1 cut(s) 143
AoxI GGCC 3 cut(s) 209, 216, 261
ApeKI GCWGC 2 cut(s) 61, 139
ApoI RAATTY 1 cut(s) 507
Asp700I GAANNNNTTC 1 cut(s) 256
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 2 cut(s) 48, 151
BccI CCATC 2 cut(s) 155, 497
BceAI ACGGC 2 cut(s) 157, 498
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 196, 332
BfoI RGCGCY 1 cut(s) 145
BisI GCNGC 3 cut(s) 62, 140, 216
BlsI GCNGC 3 cut(s) 63, 141, 217
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 184
BsaHI GRCGYC 2 cut(s) 170, 204
BsaWI WCCGGW 2 cut(s) 318, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 325
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 483
BseAI TCCGGA 1 cut(s) 318
BseGI GGATG 1 cut(s) 575
BseLI CCNNNNNNNGG 2 cut(s) 214, 325
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 63
BseX3I CGGCCG 3 cut(s) 209, 216, 261
BseXI GCAGC 2 cut(s) 48, 151
Bsh1285I CGRYCG 3 cut(s) 212, 219, 264
BshFI GGCC 3 cut(s) 211, 218, 263
BsiEI CGRYCG 3 cut(s) 212, 219, 264
BsiSI CCGG 5 cut(s) 184, 208, 212, 319, 406
BslFI GGGAC 2 cut(s) 166, 320
BslI CCNNNNNNNGG 2 cut(s) 214, 325
BsmAI GTCTC 2 cut(s) 196, 332
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 2 cut(s) 166, 320
BsnI GGCC 3 cut(s) 211, 218, 263
Bsp119I TTCGAA 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 318
BspACI CCGC 1 cut(s) 215
BspANI GGCC 3 cut(s) 211, 218, 263
BspCNI CTCAG 1 cut(s) 304
BspEI TCCGGA 1 cut(s) 318
BspQI GCTCTTC 1 cut(s) 476
BspT104I TTCGAA 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 211
BssNI GRCGYC 2 cut(s) 170, 204
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 4 cut(s) 247, 330, 405, 476
BstACI GRCGYC 2 cut(s) 170, 204
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 575
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstMAI GTCTC 2 cut(s) 196, 332
BstMCI CGRYCG 3 cut(s) 212, 219, 264
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 48, 151
BstV2I GAAGAC 1 cut(s) 600
BstZI CGGCCG 3 cut(s) 209, 216, 261
BsuRI GGCC 3 cut(s) 211, 218, 263
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 1 cut(s) 575
Cac8I GCNNGC 1 cut(s) 213
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 3 cut(s) 178, 178, 317
Csp6I GTAC 1 cut(s) 523
CviAII CATG 1 cut(s) 518
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 1 cut(s) 312
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 3 cut(s) 209, 216, 261
EagI CGGCCG 3 cut(s) 209, 216, 261
Eam1104I CTCTTC 4 cut(s) 247, 330, 405, 476
EarI CTCTTC 4 cut(s) 247, 330, 405, 476
EclXI CGGCCG 3 cut(s) 209, 216, 261
Eco47I GGWCC 1 cut(s) 316
Eco47III AGCGCT 1 cut(s) 143
Eco52I CGGCCG 3 cut(s) 209, 216, 261
EcoT22I ATGCAT 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 1 cut(s) 521
FaiI YATR 4 cut(s) 95, 331, 333, 519
FaqI GGGAC 2 cut(s) 166, 320
FatI CATG 1 cut(s) 517
FauNDI CATATG 1 cut(s) 331
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 3 cut(s) 62, 140, 216
FokI GGATG 1 cut(s) 582
Fsp4HI GCNGC 3 cut(s) 62, 140, 216
GlaI GCGC 1 cut(s) 143
GluI GCNGC 3 cut(s) 62, 140, 216
GsuI CTGGAG 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 3 cut(s) 211, 218, 263
HapII CCGG 5 cut(s) 184, 208, 212, 319, 406
HgaI GACGC 3 cut(s) 178, 178, 317
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 2 cut(s) 170, 204
Hin1II CATG 1 cut(s) 521
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 2 cut(s) 168, 459
HindII GTYRAC 2 cut(s) 168, 459
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 6 cut(s) 4, 100, 161, 293, 397, 596
HpaII CCGG 5 cut(s) 184, 208, 212, 319, 406
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 2 cut(s) 168, 459
Hpy188I TCNGA 2 cut(s) 424, 569
Hpy188III TCNNGA 5 cut(s) 89, 305, 319, 350, 489
Hpy8I GTNNAC 2 cut(s) 168, 459
Hpy99I CGWCG 3 cut(s) 169, 172, 206
HpyAV CCTTC 3 cut(s) 66, 242, 583
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 4 cut(s) 204, 364, 410, 461
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 4 cut(s) 204, 364, 410, 461
Hsp92I GRCGYC 2 cut(s) 170, 204
Hsp92II CATG 1 cut(s) 521
HspAI GCGC 1 cut(s) 142
Kpn2I TCCGGA 1 cut(s) 318
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
LguI GCTCTTC 1 cut(s) 476
LmnI GCTCC 3 cut(s) 149, 186, 582
LpnPI CCDG 8 cut(s) 197, 208, 221, 225, 332, 419, 513, 571
Lsp1109I GCAGC 2 cut(s) 48, 151
LweI GCATC 1 cut(s) 415
MaeII ACGT 4 cut(s) 204, 364, 410, 461
MboII GAAGA 7 cut(s) 59, 233, 264, 347, 392, 463, 605
MluCI AATT 2 cut(s) 269, 507
MlyI GAGTC 4 cut(s) 94, 155, 391, 590
MnlI CCTC 6 cut(s) 44, 81, 84, 307, 357, 522
Mph1103I ATGCAT 1 cut(s) 430
MroI TCCGGA 1 cut(s) 318
MroNI GCCGGC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 3 cut(s) 50, 272, 437
MspI CCGG 5 cut(s) 184, 208, 212, 319, 406
MspR9I CCNGG 1 cut(s) 184
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 1 cut(s) 184
NdeI CATATG 1 cut(s) 331
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 1 cut(s) 521
NmeAIII GCCGAG 2 cut(s) 289, 407
NsiI ATGCAT 1 cut(s) 430
NspV TTCGAA 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 170
PdiI GCCGGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 4, 293
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 3 cut(s) 63, 141, 217
PleI GAGTC 4 cut(s) 94, 155, 391, 590
PpsI GAGTC 4 cut(s) 94, 155, 391, 590
PspPI GGNCC 1 cut(s) 316
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
SalI GTCGAC 1 cut(s) 166
SapI GCTCTTC 1 cut(s) 476
SaqAI TTAA 3 cut(s) 50, 272, 437
SatI GCNGC 3 cut(s) 62, 140, 216
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 4 cut(s) 94, 155, 391, 590
ScrFI CCNGG 1 cut(s) 184
SfaNI GCATC 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 291
SgrDI CGTCGACG 1 cut(s) 166
SinI GGWCC 1 cut(s) 316
Sse9I AATT 2 cut(s) 269, 507
SsiI CCGC 1 cut(s) 215
SspI AATATT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 4 cut(s) 207, 367, 413, 464
TaqI TCGA 6 cut(s) 90, 98, 167, 201, 291, 490
TaqII GACCGA 1 cut(s) 286
TasI AATT 2 cut(s) 269, 507
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 2 cut(s) 4, 293
Tru1I TTAA 3 cut(s) 50, 272, 437
Tru9I TTAA 3 cut(s) 50, 272, 437
TseI GCWGC 2 cut(s) 61, 139
TspDTI ATGAA 3 cut(s) 17, 348, 422
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 256
ZraI GACGTC 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.