RLG00000023562

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005497
Physical Location & Seq
Forward (+)
25240750 .. 25244161
3412 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023562

Sequence Viewer

Length: 1188 bp
ATGGAAAAGAGCAACTCTTTCAAAGGCTCTCAGAGTCCATGGAGCTCCGATTTCGGCCTGGGCGCCACCGATCGGAGGCTGGCCTTTTCCCGCCAAGCCTCGTTCCACCAATACCACGACCCCCCGCACACGCCGCGCTCCATCAATCTCAACGACTCGTCGACGCCGTTTCTGTCCCGGAGCGTCTCCAGCATCGACGTCCCGCCGGGGGCGTACTCGGCGGCCGAAGAAAACGACAAGCTGTTTTTCGGCGAAGGAAGAGCTTCGGCCGAGAAACTAACCTTTTTGACGATATTCGAATCGGTCTTTCGGATCCTGAGGTCCGGAAATCGGTATATGAAGAGACTGTTCCTCTTGATTTCGCTCAACGTGGCTTATTCTACTGCCGAGTTGTGTATTGGACTCTTCACCGGACGTGTAGGTTTGGTGTCGGATGCATTTCATTTAACTTTTGGGTGTGGCTTGTTGACGTTTTCGCTGTTCGCAATGGCGGCTTCTCGAAAGAAGCCTGATGGAATCTACACTTATGGGTACAAGAGGCTTGAAGTTTTGTCAGCTTTTACCAATGCTCTGTTTCTGTTGTTTATGTCATTCTCCTTGGCTGTAGAAGCACTCCACGCATTCATACAGGATGAATCTGAGCACAAGCATTACTTGATTGTTTCGGCAGTGACAAATCTACTGGTGAATCTTATTGGTGTTTGGTTCTTCAGAAATTATGCTCGTATCAATCTTGTTTACAGGAATGCCGAAGATATGAACAACCACTCAGTTTGTCTGCACGTTCTTGCAGATTCCATTCGCAGTGCAGGGTTGATACTGGCATCCTGGTTTCTGTCTCTTGGGGTTCAGAATGCAGAAGTTTTGTGTTTTGGACTAGTTGCAGTTGCAGTTTTTATGCTTGTAATGCCACTCTTTAGAGCAACTGGTGGTATTCTGCTCCAAATGGCACCACCAAACGTTCCAACTTCAGCATTGAGCAAATGCTGGCGACAGATTACTGCCCGCGAAGATGTTTCTGAAGTTTCTCAGGCTCGGTTTTGGGAAGTTGTTCCTGGTCATGTTGTCGGCTCACTCTCAGTACAGGTCAAGAAAGGGATAGATGATCGCCCGGTTCTTCAATTGGTGCATGGGTTGTACCACGATTTGGGAATACAGGATTTGACCGTGCAAACTGACTATGTCTGA

Protein Analysis

396

Amino Acids

43.88

Weight (kDa)

7.73

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 116 - 314 4e-31 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
AccB1I GGYRCC 2 cut(s) 62, 949
AccB7I CCANNNNNTGG 1 cut(s) 1147
AccI GTMKAC 1 cut(s) 161
AccII CGCG 2 cut(s) 136, 1008
AccIII TCCGGA 1 cut(s) 323
AciI CCGC 7 cut(s) 91, 125, 134, 203, 221, 491, 1006
AclI AACGTT 1 cut(s) 960
AclWI GGATC 2 cut(s) 307, 320
AcoI YGGCCR 2 cut(s) 222, 267
AcuI CTGAAG 3 cut(s) 694, 954, 1041
AcyI GRCGYC 3 cut(s) 63, 164, 198
AfaI GTAC 4 cut(s) 215, 533, 1083, 1139
AfiI CCNNNNNNNGG 5 cut(s) 72, 75, 208, 330, 1147
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 3 cut(s) 22, 545, 1121
AhlI ACTAGT 1 cut(s) 877
AjiI CACGTC 1 cut(s) 416
AjnI CCWGG 3 cut(s) 57, 827, 1054
AluBI AGCT 4 cut(s) 45, 241, 263, 557
AluI AGCT 4 cut(s) 45, 241, 263, 557
Alw21I GWGCWC 2 cut(s) 47, 645
Alw26I GTCTC 3 cut(s) 190, 337, 843
AlwI GGATC 2 cut(s) 307, 320
Aor13HI TCCGGA 1 cut(s) 323
AoxI GGCC 4 cut(s) 55, 81, 222, 267
Asp700I GAANNNNTTC 2 cut(s) 262, 1050
AspLEI GCGC 2 cut(s) 65, 138
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 3 cut(s) 178, 207, 1112
AsuHPI GGTGA 2 cut(s) 400, 697
AsuII TTCGAA 1 cut(s) 297
AvaII GGWCC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 317
BamHI GGATCC 1 cut(s) 312
BanI GGYRCC 2 cut(s) 62, 949
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 2 cut(s) 47, 645
BccI CCATC 2 cut(s) 149, 506
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 3 cut(s) 59, 829, 1056
BcnI CCSGG 3 cut(s) 178, 207, 1112
BcoDI GTCTC 3 cut(s) 190, 337, 843
BcuI ACTAGT 1 cut(s) 877
BfaI CTAG 1 cut(s) 878
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 3 cut(s) 134, 222, 492
BlsI GCNGC 3 cut(s) 135, 223, 493
Bme1390I CCNGG 6 cut(s) 59, 178, 207, 829, 1056, 1112
Bme18I GGWCC 1 cut(s) 321
BmgBI CACGTC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 3 cut(s) 64, 314, 951
BmrFI CCNGG 6 cut(s) 59, 178, 207, 829, 1056, 1112
BmsI GCATC 3 cut(s) 201, 424, 833
BpmI CTGGAG 1 cut(s) 172
Bpu14I TTCGAA 1 cut(s) 297
BpuMI CCSGG 3 cut(s) 178, 207, 1112
BsaHI GRCGYC 3 cut(s) 63, 164, 198
BsaJI CCNNGG 4 cut(s) 38, 58, 206, 597
BsaWI WCCGGW 2 cut(s) 323, 410
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 75, 208, 330, 1147
Bse1I ACTGG 3 cut(s) 687, 825, 931
Bse21I CCTNAGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 492
BseAI TCCGGA 1 cut(s) 323
BseBI CCWGG 3 cut(s) 59, 829, 1056
BseDI CCNNGG 4 cut(s) 38, 58, 206, 597
BseGI GGATG 3 cut(s) 439, 637, 824
BseLI CCNNNNNNNGG 5 cut(s) 72, 75, 208, 330, 1147
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 6 cut(s) 44, 308, 630, 783, 1043, 1092
BseNI ACTGG 3 cut(s) 687, 825, 931
BseX3I CGGCCG 2 cut(s) 222, 267
BsgI GTGCAG 2 cut(s) 764, 828
Bsh1236I CGCG 2 cut(s) 136, 1008
Bsh1285I CGRYCG 3 cut(s) 73, 225, 270
BshFI GGCC 4 cut(s) 57, 83, 224, 269
BshNI GGYRCC 2 cut(s) 62, 949
BsiEI CGRYCG 3 cut(s) 73, 225, 270
BsiHKAI GWGCWC 2 cut(s) 47, 645
BsiSI CCGG 5 cut(s) 178, 206, 324, 411, 1112
BslFI GGGAC 2 cut(s) 160, 185
BslI CCNNNNNNNGG 5 cut(s) 72, 75, 208, 330, 1147
BsmAI GTCTC 3 cut(s) 190, 337, 843
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 160, 185
BsmI GAATGC 3 cut(s) 620, 751, 859
BsnI GGCC 4 cut(s) 57, 83, 224, 269
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 2 cut(s) 47, 645
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 3 cut(s) 70, 312, 1105
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 7 cut(s) 91, 125, 134, 203, 221, 491, 1006
BspANI GGCC 4 cut(s) 57, 83, 224, 269
BspCNI CTCAG 6 cut(s) 43, 309, 631, 782, 1042, 1091
BspEI TCCGGA 1 cut(s) 323
BspFNI CGCG 2 cut(s) 136, 1008
BspLI GGNNCC 3 cut(s) 64, 314, 951
BspPI GGATC 2 cut(s) 307, 320
BspQI GCTCTTC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 297
BspT107I GGYRCC 2 cut(s) 62, 949
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 3 cut(s) 687, 825, 931
BssECI CCNNGG 4 cut(s) 38, 58, 206, 597
BssMI GATC 3 cut(s) 70, 312, 1105
BssNI GRCGYC 3 cut(s) 63, 164, 198
BssT1I CCWWGG 2 cut(s) 38, 597
Bst2UI CCWGG 3 cut(s) 59, 829, 1056
Bst4CI ACNGT 2 cut(s) 348, 1168
Bst6I CTCTTC 3 cut(s) 253, 335, 410
BstACI GRCGYC 3 cut(s) 63, 164, 198
BstBI TTCGAA 1 cut(s) 297
BstC8I GCNNGC 3 cut(s) 81, 989, 1006
BstDEI CTNAG 6 cut(s) 30, 317, 639, 769, 1029, 1078
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 439, 637, 824
BstFNI CGCG 2 cut(s) 136, 1008
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 138
BstKTI GATC 3 cut(s) 73, 315, 1108
BstMAI GTCTC 3 cut(s) 190, 337, 843
BstMBI GATC 3 cut(s) 70, 312, 1105
BstMCI CGRYCG 3 cut(s) 73, 225, 270
BstMWI GCNNNNNNNGC 7 cut(s) 133, 189, 218, 491, 608, 617, 907
BstNI CCWGG 3 cut(s) 59, 829, 1056
BstSCI CCNGG 6 cut(s) 57, 176, 205, 827, 1054, 1110
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 2 cut(s) 136, 1008
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BstZI CGGCCG 2 cut(s) 222, 267
Bsu36I CCTNAGG 1 cut(s) 317
BsuRI GGCC 4 cut(s) 57, 83, 224, 269
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 416
BtsCI GGATG 3 cut(s) 439, 637, 824
BtsI GCAGTG 2 cut(s) 675, 811
BtsIMutI CAGTG 2 cut(s) 675, 811
Cac8I GCNNGC 3 cut(s) 81, 989, 1006
CfoI GCGC 2 cut(s) 65, 138
Cfr13I GGNCC 1 cut(s) 321
CseI GACGC 2 cut(s) 172, 172
Csp6I GTAC 4 cut(s) 214, 532, 1082, 1138
CspCI CAANNNNNGTGG 2 cut(s) 755, 790
CviAII CATG 3 cut(s) 39, 1061, 1130
CviQI GTAC 4 cut(s) 214, 532, 1082, 1138
DdeI CTNAG 6 cut(s) 30, 317, 639, 769, 1029, 1078
DinI GGCGCC 1 cut(s) 64
DpnI GATC 3 cut(s) 72, 314, 1107
DpnII GATC 3 cut(s) 70, 312, 1105
EaeI YGGCCR 2 cut(s) 222, 267
EagI CGGCCG 2 cut(s) 222, 267
Eam1104I CTCTTC 3 cut(s) 253, 335, 410
EarI CTCTTC 3 cut(s) 253, 335, 410
Ecl136II GAGCTC 1 cut(s) 45
EclXI CGGCCG 2 cut(s) 222, 267
Eco130I CCWWGG 2 cut(s) 38, 597
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 321
Eco52I CGGCCG 2 cut(s) 222, 267
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 3 cut(s) 694, 954, 1041
Eco81I CCTNAGG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 45
EcoRII CCWGG 3 cut(s) 57, 827, 1054
EcoT14I CCWWGG 2 cut(s) 38, 597
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 2 cut(s) 38, 597
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 3 cut(s) 42, 1064, 1133
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 2 cut(s) 160, 185
FatI CATG 3 cut(s) 38, 1060, 1129
FauI CCCGC 4 cut(s) 98, 132, 210, 1013
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 134, 222, 492
FokI GGATG 3 cut(s) 446, 644, 811
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 134, 222, 492
FspBI CTAG 1 cut(s) 878
GlaI GCGC 2 cut(s) 64, 137
GluI GCNGC 3 cut(s) 134, 222, 492
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 57, 83, 224, 269
HapII CCGG 5 cut(s) 178, 206, 324, 411, 1112
HgaI GACGC 2 cut(s) 172, 172
HhaI GCGC 2 cut(s) 65, 138
Hin1I GRCGYC 3 cut(s) 63, 164, 198
Hin1II CATG 3 cut(s) 42, 1064, 1133
Hin6I GCGC 2 cut(s) 63, 136
HinP1I GCGC 2 cut(s) 63, 136
HincII GTYRAC 2 cut(s) 162, 468
HindII GTYRAC 2 cut(s) 162, 468
HinfI GANTC 8 cut(s) 34, 155, 299, 402, 516, 635, 688, 794
HpaII CCGG 5 cut(s) 178, 206, 324, 411, 1112
HphI GGTGA 2 cut(s) 400, 697
Hpy166II GTNNAC 3 cut(s) 162, 468, 739
Hpy188III TCNNGA 5 cut(s) 316, 324, 355, 498, 1090
Hpy8I GTNNAC 3 cut(s) 162, 468, 739
Hpy99I CGWCG 3 cut(s) 163, 166, 200
HpyAV CCTTC 1 cut(s) 248
HpyCH4III ACNGT 2 cut(s) 348, 1168
HpyCH4IV ACGT 6 cut(s) 198, 369, 415, 470, 783, 960
HpyCH4V TGCA 9 cut(s) 437, 781, 791, 809, 857, 884, 890, 1129, 1171
HpyF10VI GCNNNNNNNGC 7 cut(s) 133, 189, 218, 491, 608, 617, 907
HpyF3I CTNAG 6 cut(s) 30, 317, 639, 769, 1029, 1078
HpySE526I ACGT 6 cut(s) 198, 369, 415, 470, 783, 960
Hsp92I GRCGYC 3 cut(s) 63, 164, 198
Hsp92II CATG 3 cut(s) 42, 1064, 1133
HspAI GCGC 2 cut(s) 63, 136
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 323
Kzo9I GATC 3 cut(s) 70, 312, 1105
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 5 cut(s) 42, 50, 143, 180, 945
LweI GCATC 3 cut(s) 201, 424, 833
MaeI CTAG 1 cut(s) 878
MaeII ACGT 6 cut(s) 198, 369, 415, 470, 783, 960
MaeIII GTNAC 1 cut(s) 670
MalI GATC 3 cut(s) 72, 314, 1107
MboI GATC 3 cut(s) 70, 312, 1105
MboII GAAGA 8 cut(s) 239, 270, 352, 397, 700, 764, 1022, 1109
MfeI CAATTG 1 cut(s) 1121
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 2 cut(s) 47, 645
MluCI AATT 2 cut(s) 715, 1121
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 43, 149, 396
MmeI TCCRAC 2 cut(s) 411, 989
MnlI CCTC 5 cut(s) 69, 109, 312, 362, 531
Mph1103I ATGCAT 1 cut(s) 439
MroI TCCGGA 1 cut(s) 323
MroXI GAANNNNTTC 2 cut(s) 262, 1050
MseI TTAA 1 cut(s) 446
MspI CCGG 5 cut(s) 178, 206, 324, 411, 1112
MspR9I CCNGG 6 cut(s) 59, 178, 207, 829, 1056, 1112
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 3 cut(s) 620, 751, 859
MvaI CCWGG 3 cut(s) 59, 829, 1056
MvnI CGCG 2 cut(s) 136, 1008
MwoI GCNNNNNNNGC 7 cut(s) 133, 189, 218, 491, 608, 617, 907
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 3 cut(s) 178, 207, 1112
NcoI CCATGG 1 cut(s) 38
NdeII GATC 3 cut(s) 70, 312, 1105
NlaIII CATG 3 cut(s) 42, 1064, 1133
NlaIV GGNNCC 3 cut(s) 64, 314, 951
NmeAIII GCCGAG 3 cut(s) 197, 295, 412
NmuCI GTSAC 1 cut(s) 670
NsiI ATGCAT 1 cut(s) 439
NspV TTCGAA 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 3 cut(s) 620, 751, 859
PdmI GAANNNNTTC 2 cut(s) 262, 1050
PfeI GAWTC 5 cut(s) 299, 516, 635, 688, 794
PflFI GACNNNGTC 1 cut(s) 1181
PflMI CCANNNNNTGG 1 cut(s) 1147
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 3 cut(s) 135, 223, 493
Ple19I CGATCG 1 cut(s) 73
PleI GAGTC 3 cut(s) 42, 149, 396
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 42, 149, 396
Psp124BI GAGCTC 1 cut(s) 47
Psp1406I AACGTT 1 cut(s) 960
Psp6I CCWGG 3 cut(s) 57, 827, 1054
PspGI CCWGG 3 cut(s) 57, 827, 1054
PspN4I GGNNCC 3 cut(s) 64, 314, 951
PspPI GGNCC 1 cut(s) 321
PsuI RGATCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 1181
PvuI CGATCG 1 cut(s) 73
RsaI GTAC 4 cut(s) 215, 533, 1083, 1139
RsaNI GTAC 4 cut(s) 214, 532, 1082, 1138
SacI GAGCTC 1 cut(s) 47
SalI GTCGAC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 3 cut(s) 134, 222, 492
Sau3AI GATC 3 cut(s) 70, 312, 1105
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 3 cut(s) 43, 149, 396
ScrFI CCNGG 6 cut(s) 59, 178, 207, 829, 1056, 1112
SduI GDGCHC 2 cut(s) 47, 645
SfaNI GCATC 3 cut(s) 201, 424, 833
SfcI CTRYAG 1 cut(s) 603
SfoI GGCGCC 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 297
SgrDI CGTCGACG 1 cut(s) 160
SinI GGWCC 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 877
Sse9I AATT 2 cut(s) 715, 1121
SsiI CCGC 7 cut(s) 91, 125, 134, 203, 221, 491, 1006
SspDI GGCGCC 1 cut(s) 62
SspMI CTAG 1 cut(s) 878
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 6 cut(s) 57, 176, 205, 827, 1054, 1110
StyI CCWWGG 2 cut(s) 38, 597
TaaI ACNGT 2 cut(s) 348, 1168
TaiI ACGT 6 cut(s) 201, 372, 418, 473, 786, 963
TaqI TCGA 4 cut(s) 161, 195, 297, 499
TaqII GACCGA 1 cut(s) 292
TasI AATT 2 cut(s) 715, 1121
TatI WGTACW 1 cut(s) 1081
TauI GCSGC 3 cut(s) 136, 224, 494
TfiI GAWTC 5 cut(s) 299, 516, 635, 688, 794
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 675, 811
TseFI GTSAC 1 cut(s) 670
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 5 cut(s) 353, 431, 613, 648, 773
TspRI CASTG 2 cut(s) 675, 811
Tth111I GACNNNGTC 1 cut(s) 1181
Van91I CCANNNNNTGG 1 cut(s) 1147
VpaK11BI GGWCC 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 161
XmnI GAANNNNTTC 2 cut(s) 262, 1050
XspI CTAG 1 cut(s) 878
ZraI GACGTC 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.