MD14G1055100.v1.1

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005497
Physical Location & Seq
Forward (+)
5606317 .. 5618702
12386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1055100.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGACAAATCCAATTCATTCAAAGGCAACCAGAATCCATGGAGCGCGGATTTCGGACTCGGCGCCACCGATCGGAGATTGGCGTTTTCCCGCCAAGCCTCGTTCCAGCAGTACCGCGAACCACCGCACACGCCTGTCTCCATCAATTTCGACGACTCCGAGGCCACGCCGTTTCTCTTCCGGAGCGTGTCGAGCATCGACGTTCCGCCGGGGGCGTATTTGACGGAGGAAAGCGACGAGCTTTTCGGGGACCGGAGCGTTTCGGCCGAGAAATTGTCGGTTTTGGTGCTTTTCGAATCGGTGTTTCGGATCTTGAGGTCCGGAAACCGGTATATGAAGAGGTTGTTCTTATTGATTTCGCTTAATGTGGCGTATTCGACAGCAGAGTTATTTATTGGACTCTTCACAGGACGTATAGGTTTAGTGTCGGATTCCTTTCATTTAAGTTTTGGTTGTGGCTTATTAACGTTTTCATTGTTCGCTATGGCGGCTTCTCGAAAGAAACCGGATGGAATTTACACTTATGGGTATAAAAGGCTTGAAGTTTTGTCTGCTTTTACCAATGCTCTGTTTCTTTTGTTTATGTCATTCTCATTGGCTGTCGAAGCACTTCACGCATTCATACAGGATGAATCTGAACACAAGCATTACTTGATTGTTTCGGCTGTGACAAATCTACTGGTGAACCTTATTGGTGTTTGGTTCTTCAGAAATTATGCTCGTGTAAATCTTGTTTATAGAAATGCAGAAGATATGAACAATCACTCAGTTTGCCTGCATGTTCTTGCAGATTCAATTCGCAGTGCAGGGGTGATATTGGCATCCTGGTTTCTATCTATTGGGGTTCAGAATGCAGAGGTTTTGTGTTTCGGATTAGTTTCAGCTGCAGTTTTTATGCTTGTAATGCCATTATTTAGAGCGAGTGGTGGTATTCTACTCCAAATGGCACCACCAAACGTTCCACCTTCAGCATTGAGCAAATGCTGGCGACAGATTACTGCCCGTGAAGATGTATCTGAAGTTTCACAAGCACGGTTTTGGGAATTGGTTCCTGGTCATGTTGTTGGGTCGATTTCAGTACAGGTAAAGAAAGGAATAGACGATCGCCCCATTCTTCAATATGTGCACGGTTTGTACCATGATTTGGGAATACAGGACTTGACAGTGCAAGCTGACAATGTCTGA

Protein Analysis

395

Amino Acids

43.88

Weight (kDa)

6.37

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 115 - 313 3.1e-31 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 946
AccB7I CCANNNNNTGG 1 cut(s) 1144
AccII CGCG 2 cut(s) 47, 117
AccIII TCCGGA 2 cut(s) 180, 320
AciI CCGC 6 cut(s) 47, 91, 115, 125, 206, 488
AclI AACGTT 2 cut(s) 467, 957
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 3 cut(s) 691, 951, 1038
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 113, 1080, 1136
AfiI CCNNNNNNNGG 3 cut(s) 72, 327, 1144
AgeI ACCGGT 1 cut(s) 327
AgsI TTSAA 4 cut(s) 22, 542, 795, 1118
AjnI CCWGG 2 cut(s) 824, 1051
AluBI AGCT 3 cut(s) 241, 884, 1172
AluI AGCT 3 cut(s) 241, 884, 1172
Alw21I GWGCWC 1 cut(s) 1128
Alw26I GTCTC 1 cut(s) 142
Alw44I GTGCAC 1 cut(s) 1124
AlwI GGATC 1 cut(s) 317
Aor13HI TCCGGA 2 cut(s) 180, 320
AoxI GGCC 2 cut(s) 162, 264
ApaLI GTGCAC 1 cut(s) 1124
ApeKI GCWGC 1 cut(s) 884
ApoI RAATTY 1 cut(s) 513
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AsiGI ACCGGT 1 cut(s) 327
Asp700I GAANNNNTTC 2 cut(s) 609, 1047
AspLEI GCGC 2 cut(s) 47, 65
AspS9I GGNCC 2 cut(s) 250, 318
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 2 cut(s) 694, 823
AsuII TTCGAA 1 cut(s) 294
AvaII GGWCC 2 cut(s) 250, 318
BaeGI GKGCMC 1 cut(s) 1128
BanI GGYRCC 2 cut(s) 62, 946
BauI CACGAG 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 1128
BbvI GCAGC 1 cut(s) 871
BccI CCATC 2 cut(s) 149, 503
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 2 cut(s) 826, 1053
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 142
BfmI CTRYAG 1 cut(s) 885
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 2 cut(s) 489, 885
BlsI GCNGC 2 cut(s) 490, 886
Bme1390I CCNGG 3 cut(s) 210, 826, 1053
Bme18I GGWCC 2 cut(s) 250, 318
BmgT120I GGNCC 2 cut(s) 250, 318
BmiI GGNNCC 4 cut(s) 64, 251, 948, 1050
BmrFI CCNGG 3 cut(s) 210, 826, 1053
BmsI GCATC 2 cut(s) 204, 830
Bpu14I TTCGAA 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 38, 159, 209
BsaWI WCCGGW 5 cut(s) 180, 252, 320, 327, 505
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 327, 1144
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 684
BseAI TCCGGA 2 cut(s) 180, 320
BseBI CCWGG 2 cut(s) 826, 1053
BseDI CCNNGG 3 cut(s) 38, 159, 209
BseGI GGATG 3 cut(s) 514, 634, 821
BseLI CCNNNNNNNGG 3 cut(s) 72, 327, 1144
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 1128
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 871
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 47, 117
Bsh1285I CGRYCG 3 cut(s) 73, 267, 1105
BshFI GGCC 2 cut(s) 164, 266
BshNI GGYRCC 2 cut(s) 62, 946
BshTI ACCGGT 1 cut(s) 327
BsiEI CGRYCG 3 cut(s) 73, 267, 1105
BsiHKAI GWGCWC 1 cut(s) 1128
BsiSI CCGG 6 cut(s) 181, 209, 253, 321, 328, 506
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 3 cut(s) 72, 327, 1144
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 263
BsmI GAATGC 2 cut(s) 617, 856
BsnI GGCC 2 cut(s) 164, 266
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 1128
Bsp13I TCCGGA 2 cut(s) 180, 320
Bsp143I GATC 3 cut(s) 70, 309, 1102
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 6 cut(s) 47, 91, 115, 125, 206, 488
BspANI GGCC 2 cut(s) 164, 266
BspCNI CTCAG 1 cut(s) 779
BspEI TCCGGA 2 cut(s) 180, 320
BspFNI CGCG 2 cut(s) 47, 117
BspLI GGNNCC 4 cut(s) 64, 251, 948, 1050
BspMAI CTGCAG 1 cut(s) 889
BspPI GGATC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 294
BspT107I GGYRCC 2 cut(s) 62, 946
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 3 cut(s) 38, 159, 209
BssMI GATC 3 cut(s) 70, 309, 1102
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 720
Bst2UI CCWGG 2 cut(s) 826, 1053
Bst4CI ACNGT 3 cut(s) 1035, 1130, 1165
Bst6I CTCTTC 3 cut(s) 182, 332, 407
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 3 cut(s) 776, 986, 1170
BstDEI CTNAG 1 cut(s) 766
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 514, 634, 821
BstFNI CGCG 2 cut(s) 47, 117
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 47, 65
BstKTI GATC 3 cut(s) 73, 312, 1105
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 3 cut(s) 70, 309, 1102
BstMCI CGRYCG 3 cut(s) 73, 267, 1105
BstMWI GCNNNNNNNGC 5 cut(s) 192, 488, 605, 614, 904
BstNI CCWGG 2 cut(s) 826, 1053
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 3 cut(s) 208, 824, 1051
BstSFI CTRYAG 1 cut(s) 885
BstSLI GKGCMC 1 cut(s) 1128
BstUI CGCG 2 cut(s) 47, 117
BstV1I GCAGC 1 cut(s) 871
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
BstZI CGGCCG 1 cut(s) 264
BsuRI GGCC 2 cut(s) 164, 266
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 514, 634, 821
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 2 cut(s) 808, 1170
Cac8I GCNNGC 3 cut(s) 776, 986, 1170
CfoI GCGC 2 cut(s) 47, 65
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 250, 318
Csp6I GTAC 3 cut(s) 112, 1079, 1135
CspAI ACCGGT 1 cut(s) 327
CviAII CATG 4 cut(s) 39, 779, 1058, 1139
CviQI GTAC 3 cut(s) 112, 1079, 1135
DdeI CTNAG 1 cut(s) 766
DinI GGCGCC 1 cut(s) 64
DpnI GATC 3 cut(s) 72, 311, 1104
DpnII GATC 3 cut(s) 70, 309, 1102
EaeI YGGCCR 1 cut(s) 264
EagI CGGCCG 1 cut(s) 264
Eam1104I CTCTTC 3 cut(s) 182, 332, 407
EarI CTCTTC 3 cut(s) 182, 332, 407
EciI GGCGGA 1 cut(s) 195
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 250, 318
Eco52I CGGCCG 1 cut(s) 264
Eco57I CTGAAG 3 cut(s) 691, 951, 1038
EcoRII CCWGG 2 cut(s) 824, 1051
EcoT14I CCWWGG 1 cut(s) 38
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 4 cut(s) 42, 782, 1061, 1142
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 263
FatI CATG 4 cut(s) 38, 778, 1057, 1138
FauI CCCGC 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 489, 885
FokI GGATG 3 cut(s) 521, 641, 808
Fsp4HI GCNGC 2 cut(s) 489, 885
GlaI GCGC 2 cut(s) 46, 64
GluI GCNGC 2 cut(s) 489, 885
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 2 cut(s) 164, 266
HapII CCGG 6 cut(s) 181, 209, 253, 321, 328, 506
HhaI GCGC 2 cut(s) 47, 65
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 4 cut(s) 42, 782, 1061, 1142
Hin6I GCGC 2 cut(s) 45, 63
HinP1I GCGC 2 cut(s) 45, 63
HinfI GANTC 8 cut(s) 34, 57, 155, 296, 399, 431, 632, 791
HpaII CCGG 6 cut(s) 181, 209, 253, 321, 328, 506
HphI GGTGA 2 cut(s) 694, 823
Hpy166II GTNNAC 2 cut(s) 685, 1126
Hpy188III TCNNGA 4 cut(s) 181, 313, 321, 495
Hpy8I GTNNAC 2 cut(s) 685, 1126
Hpy99I CGWCG 3 cut(s) 155, 203, 239
HpyAV CCTTC 1 cut(s) 975
HpyCH4III ACNGT 3 cut(s) 1035, 1130, 1165
HpyCH4IV ACGT 4 cut(s) 201, 412, 467, 957
HpyCH4V TGCA 8 cut(s) 746, 778, 788, 806, 854, 887, 1126, 1168
HpyF10VI GCNNNNNNNGC 5 cut(s) 192, 488, 605, 614, 904
HpyF3I CTNAG 1 cut(s) 766
HpySE526I ACGT 4 cut(s) 201, 412, 467, 957
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 4 cut(s) 42, 782, 1061, 1142
HspAI GCGC 2 cut(s) 45, 63
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 2 cut(s) 180, 320
Kzo9I GATC 3 cut(s) 70, 309, 1102
LmnI GCTCC 3 cut(s) 42, 183, 255
Lsp1109I GCAGC 1 cut(s) 871
LweI GCATC 2 cut(s) 204, 830
MaeII ACGT 4 cut(s) 201, 412, 467, 957
MaeIII GTNAC 1 cut(s) 667
MalI GATC 3 cut(s) 72, 311, 1104
MboI GATC 3 cut(s) 70, 309, 1102
MboII GAAGA 7 cut(s) 169, 349, 394, 697, 761, 1019, 1106
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 1128
MluCI AATT 7 cut(s) 13, 145, 272, 513, 712, 795, 1043
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 51, 149, 393
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 6 cut(s) 109, 154, 220, 309, 333, 850
MroI TCCGGA 2 cut(s) 180, 320
MroXI GAANNNNTTC 2 cut(s) 609, 1047
MseI TTAA 3 cut(s) 363, 443, 464
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 6 cut(s) 181, 209, 253, 321, 328, 506
MspR9I CCNGG 3 cut(s) 210, 826, 1053
Mva1269I GAATGC 2 cut(s) 617, 856
MvaI CCWGG 2 cut(s) 826, 1053
MvnI CGCG 2 cut(s) 47, 117
MwoI GCNNNNNNNGC 5 cut(s) 192, 488, 605, 614, 904
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 210
NcoI CCATGG 1 cut(s) 38
NdeII GATC 3 cut(s) 70, 309, 1102
NlaIII CATG 4 cut(s) 42, 782, 1061, 1142
NlaIV GGNNCC 4 cut(s) 64, 251, 948, 1050
NmeAIII GCCGAG 2 cut(s) 39, 292
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 782
NspV TTCGAA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 156
PctI GAATGC 2 cut(s) 617, 856
PdmI GAANNNNTTC 2 cut(s) 609, 1047
PfeI GAWTC 5 cut(s) 34, 296, 431, 632, 791
PflFI GACNNNGTC 1 cut(s) 1178
PflMI CCANNNNNTGG 1 cut(s) 1144
PinAI ACCGGT 1 cut(s) 327
PkrI GCNGC 2 cut(s) 490, 886
Ple19I CGATCG 2 cut(s) 73, 1105
PleI GAGTC 3 cut(s) 51, 149, 393
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 51, 149, 393
Psp1406I AACGTT 2 cut(s) 467, 957
Psp6I CCWGG 2 cut(s) 824, 1051
PspGI CCWGG 2 cut(s) 824, 1051
PspN4I GGNNCC 4 cut(s) 64, 251, 948, 1050
PspPI GGNCC 2 cut(s) 250, 318
PstI CTGCAG 1 cut(s) 889
PsuI RGATCY 1 cut(s) 309
PsyI GACNNNGTC 1 cut(s) 1178
PvuI CGATCG 2 cut(s) 73, 1105
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 3 cut(s) 113, 1080, 1136
RsaNI GTAC 3 cut(s) 112, 1079, 1135
SaqAI TTAA 3 cut(s) 363, 443, 464
SatI GCNGC 2 cut(s) 489, 885
Sau3AI GATC 3 cut(s) 70, 309, 1102
Sau96I GGNCC 2 cut(s) 250, 318
SchI GAGTC 3 cut(s) 51, 149, 393
ScrFI CCNGG 3 cut(s) 210, 826, 1053
SduI GDGCHC 1 cut(s) 1128
SfaNI GCATC 2 cut(s) 204, 830
SfcI CTRYAG 1 cut(s) 885
SfoI GGCGCC 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 294
SinI GGWCC 2 cut(s) 250, 318
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 7 cut(s) 13, 145, 272, 513, 712, 795, 1043
SsiI CCGC 6 cut(s) 47, 91, 115, 125, 206, 488
SspDI GGCGCC 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 208, 824, 1051
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 3 cut(s) 1035, 1130, 1165
TaiI ACGT 4 cut(s) 204, 415, 470, 960
TaqI TCGA 8 cut(s) 150, 191, 198, 294, 377, 496, 603, 1070
TasI AATT 7 cut(s) 13, 145, 272, 513, 712, 795, 1043
TatI WGTACW 1 cut(s) 1078
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 5 cut(s) 34, 296, 431, 632, 791
Tru1I TTAA 3 cut(s) 363, 443, 464
Tru9I TTAA 3 cut(s) 363, 443, 464
TscAI CASTG 2 cut(s) 808, 1170
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 1 cut(s) 884
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 7 cut(s) 6, 350, 428, 462, 610, 645, 770
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 2 cut(s) 808, 1170
Tth111I GACNNNGTC 1 cut(s) 1178
Van91I CCANNNNNTGG 1 cut(s) 1144
VneI GTGCAC 1 cut(s) 1124
VpaK11BI GGWCC 2 cut(s) 250, 318
XapI RAATTY 1 cut(s) 513
XceI RCATGY 1 cut(s) 782
XmnI GAANNNNTTC 2 cut(s) 609, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.