AT3G20730

repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005777
Physical Location & Seq
Forward (+)
7246896 .. 7248878
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20730.1

Sequence Viewer

Length: 1698 bp
ATGATGCTGGTTGTTTCGGAAAACTACTTATTAAGCCCTTCTTTGTACCTTAAAGCTCTTAAATTATGCAGCTACCAAAACGTCAAGAAACAACTATTGTTGATCCATGGGAATTCGATTACAAATGGGTTCTGCTCCAATCTCCAGTTGAAGGACATGTTGATAGATTTGTACTTAAAACAGGGAGATGTGAAACATGCACGTAAACTGTTCGATAGAATTTCTAAGAGAGATGTGGTTTCTTGGACAGCTATGATCTCGAGGTTTTCTCGATGTGGATATCACCCGGACGCGTTGTTGTTGTTTAAAGAGATGCACCGGGAAGATGTTAAGGCTAATCAGTTCACTTATGGGAGTGTTTTGAAGTCGTGTAAAGATTTGGGGTGTCTGAAAGAAGGGATGCAGATACATGGTAGTGTGGAGAAAGGGAATTGTGCAGGGAACTTAATTGTGAGAAGTGCATTGCTTTCTCTTTATGCCAGGTGTGGAAAGATGGAGGAAGCTCGTCTTCAGTTTGATTCAATGAAAGAAAGGGATTTGGTTTCTTGGAATGCTATGATCGATGGATACACCGCCAATGCTTGTGCAGATACTTCGTTTAGTCTGTTTCAGTTGATGCTCACGGAAGGGAAGAAGCCAGACTGCTTCACCTTTGGAAGCCTCTTGAGAGCTTCTATTGTCGTTAAGTGTCTTGAAATAGTCAGTGAATTACATGGATTAGCGATCAAGCTGGGTTTTGGGAGATCAAGTGCACTAATTAGGTCTCTGGTTAATGCATATGTGAAGTGTGGTAGCCTTGCCAATGCTTGGAAACTGCACGAGGGCACAAAGAAAAGAGACTTACTATCTTGCACTGCTCTAATTACAGGTTTTTCACAGCAGAACAACTGCACAAGTGATGCCTTTGATATCTTCAAAGATATGATACGGATGAAAACAAAGATGGACGAAGTTGTAGTATCCTCGATGCTTAAAATATGCACCACCATTGCATCTGTAACCATTGGAAGACAGATCCATGGTTTTGCCTTAAAATCTTCCCAAATCAGATTCGATGTTGCTTTGGGGAATTCGCTGATAGATATGTATGCAAAGTCTGGGGAAATCGAGGATGCAGTCCTGGCTTTCGAAGAGATGAAAGAGAAAGATGTGAGATCATGGACCTCTTTGATTGCAGGATATGGAAGACATGGGAATTTTGAGAAAGCAATTGATCTTTATAACAGAATGGAGCATGAAAGGATCAAACCGAATGACGTTACATTTCTTTCTCTCCTCTCTGCTTGTAGTCATACAGGACAAACCGAACTAGGCTGGAAGATTTACGACACAATGATCAACAAGCACGGAATCGAAGCTCGAGAAGAGCACTTATCTTGCATCATAGATATGCTTGCGCGCAGTGGCTACTTGGAAGAAGCTTATGCACTGATAAGAAGTAAAGAGGGTATTGTAAGTCTTAGCTCATCAACATGGGGAGCTTTTCTTGATGCATGCAGGCGCCATGGGAATGTGCAGCTCAGTAAAGTAGCAGCCACGCAGCTTCTGAGTATGGAACCGAGGAAACCAGTCAATTACATCAATTTGGCCAGTGTGTATGCAGCTAATGGAGCATGGGACAATGCTTTGAATACTAGAAAGCTTATGAAAGAGAGTGGTTCCTGCAATAAAGCTCCAGGATACAGCCTTGTGTATTGA

Protein Analysis

565

Amino Acids

63.11

Weight (kDa)

8.87

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 77 - 124 5.8e-10 PPR repeat family
PPR PF01535 80 - 109 4e-06 PPR repeat
PPR_2 PF13041 179 - 224 2e-07 PPR repeat family
PPR_long PF17177 298 - 436 6.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 383 - 430 2.1e-13 PPR repeat family
PPR_3 PF13812 383 - 429 7.8e-06 Pentatricopeptide repeat domain
PPR PF01535 386 - 414 3.8e-08 PPR repeat
E_motif PF20431 502 - 564 1.5e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AccB1I GGYRCC 1 cut(s) 1500
AccB7I CCANNNNNTGG 1 cut(s) 807
AccII CGCG 2 cut(s) 293, 1399
AciI CCGC 1 cut(s) 573
AclWI GGATC 3 cut(s) 97, 1009, 1250
AcoI YGGCCR 1 cut(s) 1587
AcsI RAATTY 4 cut(s) 112, 219, 1069, 1195
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 1501
AfaI GTAC 2 cut(s) 47, 173
AfiI CCNNNNNNNGG 2 cut(s) 151, 807
AflIII ACRYGT 2 cut(s) 156, 291
AgsI TTSAA 6 cut(s) 151, 364, 522, 695, 916, 1630
AjnI CCWGG 3 cut(s) 479, 1119, 1675
Alw21I GWGCWC 2 cut(s) 754, 1371
Alw26I GTCTC 2 cut(s) 768, 831
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 3 cut(s) 97, 1009, 1250
AlwNI CAGNNNCTG 1 cut(s) 1546
Ama87I CYCGRG 2 cut(s) 259, 1359
AoxI GGCC 1 cut(s) 1587
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 5 cut(s) 69, 1516, 1532, 1540, 1601
ApoI RAATTY 4 cut(s) 112, 219, 1069, 1195
AspLEI GCGC 3 cut(s) 1399, 1401, 1503
AspS9I GGNCC 1 cut(s) 1161
AsuC2I CCSGG 2 cut(s) 287, 320
AsuHPI GGTGA 2 cut(s) 275, 640
AsuII TTCGAA 1 cut(s) 1128
AvaI CYCGRG 2 cut(s) 259, 1359
AvaII GGWCC 1 cut(s) 1161
BaeGI GKGCMC 2 cut(s) 754, 827
BalI TGGCCA 1 cut(s) 1589
BanI GGYRCC 1 cut(s) 1500
BauI CACGAG 1 cut(s) 818
BbsI GAAGAC 3 cut(s) 500, 1015, 1192
Bbv12I GWGCWC 2 cut(s) 754, 1371
BbvI GCAGC 5 cut(s) 81, 1528, 1544, 1552, 1613
BccI CCATC 3 cut(s) 487, 557, 937
BcgI CGANNNNNNTGC 2 cut(s) 576, 610
BciT130I CCWGG 3 cut(s) 481, 1121, 1677
BciVI GTATCC 3 cut(s) 560, 970, 1673
BclI TGATCA 1 cut(s) 1335
BcnI CCSGG 2 cut(s) 287, 320
BcoDI GTCTC 2 cut(s) 768, 831
BfaI CTAG 2 cut(s) 1310, 1635
BfoI RGCGCY 1 cut(s) 1504
BfuI GTATCC 3 cut(s) 560, 970, 1673
BisI GCNGC 5 cut(s) 70, 1517, 1533, 1541, 1602
BlsI GCNGC 5 cut(s) 71, 1518, 1534, 1542, 1603
Bme1390I CCNGG 5 cut(s) 287, 320, 481, 1121, 1677
Bme18I GGWCC 1 cut(s) 1161
BmeT110I CYCGRG 2 cut(s) 259, 1359
BmgT120I GGNCC 1 cut(s) 1161
BmiI GGNNCC 3 cut(s) 1502, 1557, 1660
BmrFI CCNGG 5 cut(s) 287, 320, 481, 1121, 1677
BmsI GCATC 9 cut(s) 303, 390, 606, 889, 957, 1001, 1102, 1389, 1480
BpiI GAAGAC 3 cut(s) 500, 1015, 1192
BplI GAGNNNNNCTC 2 cut(s) 253, 285
BpmI CTGGAG 2 cut(s) 128, 1659
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 685
BpuMI CCSGG 2 cut(s) 287, 320
Bsa29I ATCGAT 1 cut(s) 561
BsaAI YACGTR 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 1501
BsaI GGTCTC 1 cut(s) 768
BsaJI CCNNGG 4 cut(s) 106, 1018, 1504, 1559
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 807
Bse1I ACTGG 3 cut(s) 145, 1568, 1590
Bse3DI GCAATG 2 cut(s) 461, 987
BseBI CCWGG 3 cut(s) 481, 1121, 1677
BseCI ATCGAT 1 cut(s) 561
BseDI CCNNGG 4 cut(s) 106, 1018, 1504, 1559
BseGI GGATG 3 cut(s) 405, 936, 1117
BseLI CCNNNNNNNGG 2 cut(s) 151, 807
BseMI GCAATG 2 cut(s) 461, 987
BseMII CTCAG 2 cut(s) 1534, 1538
BseNI ACTGG 3 cut(s) 145, 1568, 1590
BsePI GCGCGC 1 cut(s) 1397
BseRI GAGGAG 1 cut(s) 1265
BseSI GKGCMC 2 cut(s) 754, 827
BseXI GCAGC 5 cut(s) 81, 1528, 1544, 1552, 1613
BseYI CCCAGC 1 cut(s) 730
BsgI GTGCAG 5 cut(s) 456, 606, 800, 874, 1535
Bsh1236I CGCG 2 cut(s) 293, 1399
BshFI GGCC 1 cut(s) 1589
BshNI GGYRCC 1 cut(s) 1500
BshVI ATCGAT 1 cut(s) 561
BsiHKAI GWGCWC 2 cut(s) 754, 1371
BsiHKCI CYCGRG 2 cut(s) 259, 1359
BsiSI CCGG 2 cut(s) 287, 319
BslFI GGGAC 1 cut(s) 1631
BslI CCNNNNNNNGG 2 cut(s) 151, 807
BsmAI GTCTC 2 cut(s) 768, 831
BsmFI GGGAC 1 cut(s) 1631
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 1 cut(s) 1589
Bso31I GGTCTC 1 cut(s) 768
BsoBI CYCGRG 2 cut(s) 259, 1359
Bsp119I TTCGAA 1 cut(s) 1128
Bsp1286I GDGCHC 3 cut(s) 754, 827, 1371
Bsp19I CCATGG 3 cut(s) 106, 1018, 1504
BspACI CCGC 1 cut(s) 573
BspANI GGCC 1 cut(s) 1589
BspCNI CTCAG 2 cut(s) 1533, 1539
BspDI ATCGAT 1 cut(s) 561
BspFNI CGCG 2 cut(s) 293, 1399
BspLI GGNNCC 3 cut(s) 1502, 1557, 1660
BspPI GGATC 3 cut(s) 97, 1009, 1250
BspQI GCTCTTC 1 cut(s) 1359
BspT104I TTCGAA 1 cut(s) 1128
BspT107I GGYRCC 1 cut(s) 1500
BspTNI GGTCTC 1 cut(s) 768
BsrDI GCAATG 2 cut(s) 461, 987
BsrI ACTGG 3 cut(s) 145, 1568, 1590
BssECI CCNNGG 4 cut(s) 106, 1018, 1504, 1559
BssHII GCGCGC 1 cut(s) 1397
BssNI GRCGYC 1 cut(s) 1501
BssSI CACGAG 1 cut(s) 818
BssT1I CCWWGG 3 cut(s) 106, 1018, 1504
Bst2BI CACGAG 1 cut(s) 818
Bst2UI CCWGG 3 cut(s) 481, 1121, 1677
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 2 cut(s) 1125, 1359
BstACI GRCGYC 1 cut(s) 1501
BstBAI YACGTR 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 4 cut(s) 1395, 1399, 1495, 1499
BstDEI CTNAG 4 cut(s) 225, 1460, 1520, 1547
BstDSI CCRYGG 3 cut(s) 106, 1018, 1504
BstF5I GGATG 3 cut(s) 405, 936, 1117
BstFNI CGCG 2 cut(s) 293, 1399
BstH2I RGCGCY 1 cut(s) 1504
BstHHI GCGC 3 cut(s) 1399, 1401, 1503
BstMAI GTCTC 2 cut(s) 768, 831
BstMWI GCNNNNNNNGC 2 cut(s) 1121, 1610
BstNI CCWGG 3 cut(s) 481, 1121, 1677
BstNSI RCATGY 3 cut(s) 160, 200, 1497
BstSCI CCNGG 5 cut(s) 285, 318, 479, 1119, 1675
BstSLI GKGCMC 2 cut(s) 754, 827
BstUI CGCG 2 cut(s) 293, 1399
BstV1I GCAGC 5 cut(s) 81, 1528, 1544, 1552, 1613
BstV2I GAAGAC 3 cut(s) 500, 1015, 1192
BstX2I RGATCY 1 cut(s) 1014
BstYI RGATCY 1 cut(s) 1014
Bsu15I ATCGAT 1 cut(s) 561
BsuI GTATCC 3 cut(s) 560, 970, 1673
BsuRI GGCC 1 cut(s) 1589
BsuTUI ATCGAT 1 cut(s) 561
BtgI CCRYGG 3 cut(s) 106, 1018, 1504
BtsCI GGATG 3 cut(s) 405, 936, 1117
BtsI GCAGTG 2 cut(s) 852, 1408
BtsIMutI CAGTG 5 cut(s) 709, 852, 1408, 1427, 1597
Cac8I GCNNGC 4 cut(s) 1395, 1399, 1495, 1499
CaiI CAGNNNCTG 1 cut(s) 1546
CfoI GCGC 3 cut(s) 1399, 1401, 1503
Cfr13I GGNCC 1 cut(s) 1161
ClaI ATCGAT 1 cut(s) 561
CseI GACGC 1 cut(s) 299
Csp6I GTAC 2 cut(s) 46, 172
CviQI GTAC 2 cut(s) 46, 172
DdeI CTNAG 4 cut(s) 225, 1460, 1520, 1547
DinI GGCGCC 1 cut(s) 1502
DraI TTTAAA 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 1587
Eam1104I CTCTTC 2 cut(s) 1125, 1359
EarI CTCTTC 2 cut(s) 1125, 1359
Eco130I CCWWGG 3 cut(s) 106, 1018, 1504
Eco31I GGTCTC 1 cut(s) 768
Eco32I GATATC 2 cut(s) 281, 910
Eco47I GGWCC 1 cut(s) 1161
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 2 cut(s) 259, 1359
EcoRI GAATTC 2 cut(s) 112, 1069
EcoRII CCWGG 3 cut(s) 479, 1119, 1675
EcoRV GATATC 2 cut(s) 281, 910
EcoT14I CCWWGG 3 cut(s) 106, 1018, 1504
EcoT22I ATGCAT 2 cut(s) 778, 1495
EgeI GGCGCC 1 cut(s) 1502
EheI GGCGCC 1 cut(s) 1502
ErhI CCWWGG 3 cut(s) 106, 1018, 1504
FalI AAGNNNNNCTT 4 cut(s) 25, 57, 492, 524
FaqI GGGAC 1 cut(s) 1631
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 1335
Fnu4HI GCNGC 5 cut(s) 70, 1517, 1533, 1541, 1602
FokI GGATG 3 cut(s) 412, 943, 1124
Fsp4HI GCNGC 5 cut(s) 70, 1517, 1533, 1541, 1602
FspBI CTAG 2 cut(s) 1310, 1635
GlaI GCGC 3 cut(s) 1398, 1400, 1502
GluI GCNGC 5 cut(s) 70, 1517, 1533, 1541, 1602
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 2 cut(s) 128, 1659
HaeII RGCGCY 1 cut(s) 1504
HaeIII GGCC 1 cut(s) 1589
HapII CCGG 2 cut(s) 287, 319
HgaI GACGC 1 cut(s) 299
HhaI GCGC 3 cut(s) 1399, 1401, 1503
Hin1I GRCGYC 1 cut(s) 1501
Hin6I GCGC 3 cut(s) 1397, 1399, 1501
HinP1I GCGC 3 cut(s) 1397, 1399, 1501
HindIII AAGCTT 2 cut(s) 1419, 1640
HinfI GANTC 3 cut(s) 518, 1050, 1350
HpaII CCGG 2 cut(s) 287, 319
HphI GGTGA 2 cut(s) 275, 640
Hpy166II GTNNAC 3 cut(s) 206, 345, 752
Hpy188I TCNGA 4 cut(s) 19, 390, 1049, 1548
Hpy188III TCNNGA 7 cut(s) 85, 259, 270, 664, 692, 1361, 1487
Hpy8I GTNNAC 3 cut(s) 206, 345, 752
HpyAV CCTTC 4 cut(s) 48, 145, 389, 620
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 3 cut(s) 81, 202, 1257
HpyF10VI GCNNNNNNNGC 2 cut(s) 1121, 1610
HpyF3I CTNAG 4 cut(s) 225, 1460, 1520, 1547
HpySE526I ACGT 3 cut(s) 81, 202, 1257
Hsp92I GRCGYC 1 cut(s) 1501
HspAI GCGC 3 cut(s) 1397, 1399, 1501
KasI GGCGCC 1 cut(s) 1500
Ksp22I TGATCA 1 cut(s) 1335
LguI GCTCTTC 1 cut(s) 1359
LmnI GCTCC 5 cut(s) 140, 1231, 1478, 1610, 1678
Lsp1109I GCAGC 5 cut(s) 81, 1528, 1544, 1552, 1613
LweI GCATC 9 cut(s) 303, 390, 606, 889, 957, 1001, 1102, 1389, 1480
MaeI CTAG 2 cut(s) 1310, 1635
MaeII ACGT 3 cut(s) 81, 202, 1257
MaeIII GTNAC 2 cut(s) 997, 1258
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 1 cut(s) 1014
MhlI GDGCHC 3 cut(s) 754, 827, 1371
MlsI TGGCCA 1 cut(s) 1589
MluI ACGCGT 1 cut(s) 291
MluNI TGGCCA 1 cut(s) 1589
Mly113I GGCGCC 1 cut(s) 1501
Mox20I TGGCCA 1 cut(s) 1589
Mph1103I ATGCAT 2 cut(s) 778, 1495
MscI TGGCCA 1 cut(s) 1589
MslI CAYNNNNRTG 4 cut(s) 414, 1388, 1471, 1509
Msp20I TGGCCA 1 cut(s) 1589
MspI CCGG 2 cut(s) 287, 319
MspR9I CCNGG 5 cut(s) 287, 320, 481, 1121, 1677
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 3 cut(s) 481, 1121, 1677
MvnI CGCG 2 cut(s) 293, 1399
MwoI GCNNNNNNNGC 2 cut(s) 1121, 1610
NarI GGCGCC 1 cut(s) 1501
NciI CCSGG 2 cut(s) 287, 320
NcoI CCATGG 3 cut(s) 106, 1018, 1504
NdeI CATATG 1 cut(s) 778
NlaIV GGNNCC 3 cut(s) 1502, 1557, 1660
NsiI ATGCAT 2 cut(s) 778, 1495
NspI RCATGY 3 cut(s) 160, 200, 1497
NspV TTCGAA 1 cut(s) 1128
PaeI GCATGC 1 cut(s) 1497
PaeR7I CTCGAG 2 cut(s) 259, 1359
PauI GCGCGC 1 cut(s) 1397
PciI ACATGT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 1359
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 518, 1050, 1350
PflMI CCANNNNNTGG 1 cut(s) 807
PfoI TCCNGGA 1 cut(s) 1675
PkrI GCNGC 5 cut(s) 71, 1518, 1534, 1542, 1603
PluTI GGCGCC 1 cut(s) 1504
Ppu21I YACGTR 1 cut(s) 203
PscI ACATGT 1 cut(s) 156
PsiI TTATAA 1 cut(s) 1221
Psp6I CCWGG 3 cut(s) 479, 1119, 1675
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 3 cut(s) 479, 1119, 1675
PspN4I GGNNCC 3 cut(s) 1502, 1557, 1660
PspPI GGNCC 1 cut(s) 1161
PstNI CAGNNNCTG 1 cut(s) 1546
PsuI RGATCY 1 cut(s) 1014
PteI GCGCGC 1 cut(s) 1397
RsaI GTAC 2 cut(s) 47, 173
RsaNI GTAC 2 cut(s) 46, 172
RseI CAYNNNNRTG 4 cut(s) 414, 1388, 1471, 1509
SapI GCTCTTC 1 cut(s) 1359
SatI GCNGC 5 cut(s) 70, 1517, 1533, 1541, 1602
Sau96I GGNCC 1 cut(s) 1161
ScrFI CCNGG 5 cut(s) 287, 320, 481, 1121, 1677
SduI GDGCHC 3 cut(s) 754, 827, 1371
SfaNI GCATC 9 cut(s) 303, 390, 606, 889, 957, 1001, 1102, 1389, 1480
SfoI GGCGCC 1 cut(s) 1502
Sfr274I CTCGAG 2 cut(s) 259, 1359
SfuI TTCGAA 1 cut(s) 1128
SinI GGWCC 1 cut(s) 1161
SlaI CTCGAG 2 cut(s) 259, 1359
SmiMI CAYNNNNRTG 4 cut(s) 414, 1388, 1471, 1509
SmlI CTYRAG 3 cut(s) 259, 664, 1359
SmoI CTYRAG 3 cut(s) 259, 664, 1359
SphI GCATGC 1 cut(s) 1497
SsiI CCGC 1 cut(s) 573
SspDI GGCGCC 1 cut(s) 1500
SspMI CTAG 2 cut(s) 1310, 1635
StyD4I CCNGG 5 cut(s) 285, 318, 479, 1119, 1675
StyI CCWWGG 3 cut(s) 106, 1018, 1504
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 3 cut(s) 84, 205, 1260
TatI WGTACW 1 cut(s) 171
TfiI GAWTC 3 cut(s) 518, 1050, 1350
TscAI CASTG 5 cut(s) 709, 859, 1408, 1434, 1597
TseI GCWGC 5 cut(s) 69, 1516, 1532, 1540, 1601
TspDTI ATGAA 5 cut(s) 539, 947, 1151, 1251, 1661
TspGWI ACGGA 3 cut(s) 638, 943, 1362
TspRI CASTG 5 cut(s) 709, 859, 1408, 1434, 1597
Van91I CCANNNNNTGG 1 cut(s) 807
VneI GTGCAC 1 cut(s) 750
VpaK11BI GGWCC 1 cut(s) 1161
XapI RAATTY 4 cut(s) 112, 219, 1069, 1195
XceI RCATGY 3 cut(s) 160, 200, 1497
XhoI CTCGAG 2 cut(s) 259, 1359
XspI CTAG 2 cut(s) 1310, 1635
Zsp2I ATGCAT 2 cut(s) 778, 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.