Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005777

Physical Location & Seq

Genomic Coordinates Reverse (-)

19968522 .. 19970392

Gene Structure 1871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G016000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGCAAGCATTGTGGAAAAGGAGTCACCTTCAAGAAGCTCTGAGACTTGTTCAGTCTAACCCAACTCACTTGGACCATTACTATTGCTTGTATATGAAGATTCTGCAACTCTGCATCGATCAACGAGTAGAAAGACCCGGTCTTTTGGCTCACACCCATGTGATCACAAATGGGTTTGCTTCAAATCTGAATTTGAACACCAAATTGATCAACTTTTATTGCAAGTTTGGCCGCGTGGTTGGTGCTCGCAAGGTGTTCGATAAAATGCCTGAGAGAAATGTTGTGTCTTGGACTTCTATGGTTTCTGGGTATGCTCAAAGTGGAAGCTATGAGAAAGCTTTGGGGGTGTTTTTGGAAATGCGTCGGGCGGGTTTCAGAGCTAATCAGTTTGGGTATGGGAGTGCATTGAGGTCATGCACTGGTTTGAGGTCTTTGGAAGTAGGAATGCAGATACAGGGGTGTGTTGTGAAAGGAAGGTTTGCTGAGAATTTGTTTGTGCTGAGTGCATTAGTTGATTTTCATGCCAAGTGTGGAAAGATAGAGGATGCTTGTTGTGTTTTCGAGGCAATGTCGGAAAGGGACTTGGTTTCTTGGAATGCAATCATTGGAGGGTATGCTGTTCAGGGTTTTAGTGACGACTCGTTGCGAATGTTTCGGTCAATGATGAGGGAAGGCATGGTTCCTGATTGCTTCACCTTCGGGAGTGTTTTGAGAGGCTTGGCCGGAGATAGTGGTTTGGTGAAGGTTAGTCAAATGCATGGATTGATAATGCATCTGGGTTTTGGATCACACAAAAGTTTGAGTGGATCGCTAATCAATGCATATGCAAAATGTGGGAAGGTAGAGTTTGCCCATAAGATATACAGAAGTATGATAGAAAAGGACATTATATCTTGCACTGCACTAATTACTGGGTATGCACGAGAAGGTAACTACAGCAGAGATGTGCTAGATCTCTTCAGGGAGATAACTGTTATGAGCATGAGACTGGATGGTGTCATATTGTGCTCCATGCTTAATATATGTGCAAATGTTGCTTCATTGATCTTGGGAAGACAAATCCATGCACTTATGTTGAAACACCAACCTTATCTTGATGTGGCCATGGGAAATGCTCTAGTTGATATGTATGCAAAATGTGGAGAGATTGAAGATGCTAACCATGCTTTTGAGGAGATGGAGGAAAAGAATGTAATTTCATGGACGTCTCTGATTTCTGGATATGGAAGGAACGGCCATGGAGCTAAAGCAATTGCATTGTATAAAAGGATGGAACTTGAGGGATTGAAACCTAATGATATCACATTCTTATCTCTTCTCTTTGCTTGTAGCCATACTGGATTGACAAGTGAAGGATGGGAATGCTTCAATACTATGCTTAGCAAATACAACATCTTGCCTAGGGCTGAGCATTTTTCTTGCTTAGTAGATCTTTATGCACGTGCAGGTTTGCTGGAAGATGTGCATGAGTTGATATGTGACATGAACATTAAGCCTGATCCCTCACTTTGGGGAGCTGTTCTTGGGGGATGTAGCACCTATGGTAATAAGTCACTTGGAGAAGTGGCATCAATGCATCTTTGTGATATTGATCCTGAGAATTCAGTTAACTATGTTGTTCTTGGAAGCATCTATGCTGCATCTGGTGCATGGGACAATGCTTTGGAAACACGAGATTTGTTAGAGAAGAGAAGTTTGAAAAAAGAACCAGCGCACAGCCTTATACATTCCAGAACAAATAATATGTTGCTTTTGCAGCCACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 peroxidase

Protein Analysis

588

Amino Acids

65.38

Weight (kDa)

7.25

Isoelectric Point (pI)

46.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	92 - 139	7.1e-08	PPR repeat family
PPR	PF01535	95 - 125	6.7e-09	PPR repeat
PPR_2	PF13041	194 - 241	6.7e-09	PPR repeat family
PPR_2	PF13041	396 - 444	2.2e-11	PPR repeat family
PPR	PF01535	399 - 429	5.1e-07	PPR repeat
E_motif	PF20431	514 - 575	1e-08	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20730
fragaria_vesca	FvH4_3g15060 FvH4_3g15060
malus_domestica	MD05G1227700.v1.1 MD10G1208100.v1.1 MD10G1208200.v1.1
prunus_persica	Prupe.4G134800_v2.0.a1
pyrus_communis	pycom05g20980 pycom05g20990 pycom10g17710
rosa_chinensis	RchiOBHm_Chr5g0024821 RchiOBHm_Chr5g0024861
rosa_laevigata	RLG00000032817
rosa_multiflora	Rmu_co8198802.1_g000001 Rmu_co8370541.1_g000001 Rmu_sc0009744.1_g000006 Rmu_sc0009744.1_g000007
rosa_roxburghii	Rroxscaffold_1G00054560
rosa_rugosa	Rorug05G0084700.1
rosa_samantha	Rh5AG176500 Rh5AG176600 Rh5BG174200 Rh5CG191200 Rh5CG191300 Rh5CG191500 Rh5DG175800 Rh5DG176000
rosa_wichuraiana	Rw5G016000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1208
Acc36I	ACCTGC	1 cut(s)	1436
AccII	CGCG	1 cut(s)	234
AciI	CCGC	2 cut(s)	232, 368
AclWI	GGATC	4 cut(s)	793, 814, 1493, 1586
AcoI	YGGCCR	4 cut(s)	229, 720, 1101, 1234
AcsI	RAATTY	3 cut(s)	190, 487, 1600
AcuI	CTGAAG	1 cut(s)	943
AcvI	CACGTG	1 cut(s)	1442
AcyI	GRCGYC	1 cut(s)	1205
AfiI	CCNNNNNNNGG	1 cut(s)	1509
AgsI	TTSAA	8 cut(s)	32, 183, 196, 1078, 1151, 1288, 1369, 1699
AleI	CACNNNNGTG	1 cut(s)	158
AluBI	AGCT	6 cut(s)	38, 327, 338, 380, 1244, 1517
AluI	AGCT	6 cut(s)	38, 327, 338, 380, 1244, 1517
Alw21I	GWGCWC	2 cut(s)	247, 1010
Alw26I	GTCTC	3 cut(s)	37, 979, 1212
AlwI	GGATC	4 cut(s)	793, 814, 1493, 1586
AoxI	GGCC	4 cut(s)	229, 720, 1101, 1234
ApeKI	GCWGC	2 cut(s)	1637, 1756
ApoI	RAATTY	3 cut(s)	190, 487, 1600
AspA2I	CCTAGG	1 cut(s)	1400
AspLEI	GCGC	1 cut(s)	1714
AspS9I	GGNCC	1 cut(s)	73
AsuC2I	CCSGG	1 cut(s)	138
AsuHPI	GGTGA	3 cut(s)	17, 685, 751
AvaII	GGWCC	1 cut(s)	73
AvrII	CCTAGG	1 cut(s)	1400
BaeI	ACNNNNGTAYC	2 cut(s)	443, 476
BalI	TGGCCA	1 cut(s)	1103
BauI	CACGAG	2 cut(s)	921, 1671
BbrPI	CACGTG	1 cut(s)	1442
BbsI	GAAGAC	1 cut(s)	1060
Bbv12I	GWGCWC	2 cut(s)	247, 1010
BbvI	GCAGC	1 cut(s)	1624
BccI	CCATC	4 cut(s)	986, 1171, 1264, 1350
BceAI	ACGGC	1 cut(s)	1249
BcgI	CGANNNNNNTGC	2 cut(s)	238, 272
BclI	TGATCA	2 cut(s)	162, 207
BcnI	CCSGG	1 cut(s)	138
BcoDI	GTCTC	3 cut(s)	37, 979, 1212
BfaI	CTAG	3 cut(s)	950, 1118, 1401
BfmI	CTRYAG	1 cut(s)	934
BfuAI	ACCTGC	1 cut(s)	1436
BglII	AGATCT	2 cut(s)	952, 1429
BisI	GCNGC	3 cut(s)	232, 1638, 1757
BlnI	CCTAGG	1 cut(s)	1400
BlpI	GCTNAGC	2 cut(s)	1379, 1407
BlsI	GCNGC	3 cut(s)	233, 1639, 1758
Bme1390I	CCNGG	1 cut(s)	138
Bme18I	GGWCC	1 cut(s)	73
BmgT120I	GGNCC	1 cut(s)	73
BmiI	GGNNCC	1 cut(s)	681
BmrFI	CCNGG	1 cut(s)	138
BmrI	ACTGGG	1 cut(s)	921
BmsI	GCATC	8 cut(s)	123, 535, 781, 1144, 1577, 1585, 1638, 1649
BmuI	ACTGGG	1 cut(s)	921
BpiI	GAAGAC	1 cut(s)	1060
Bpu1102I	GCTNAGC	2 cut(s)	1379, 1407
BpuEI	CTTGAG	1 cut(s)	1298
BpuMI	CCSGG	1 cut(s)	138
Bsa29I	ATCGAT	1 cut(s)	117
BsaAI	YACGTR	1 cut(s)	1442
BsaBI	GATNNNNATC	1 cut(s)	1590
BsaHI	GRCGYC	1 cut(s)	1205
BsaJI	CCNNGG	3 cut(s)	1104, 1237, 1400
Bsc4I	CCNNNNNNNGG	1 cut(s)	1509
Bse1I	ACTGG	4 cut(s)	424, 916, 993, 1342
Bse3DI	GCAATG	1 cut(s)	573
Bse8I	GATNNNNATC	1 cut(s)	1590
BseCI	ATCGAT	1 cut(s)	117
BseDI	CCNNGG	3 cut(s)	1104, 1237, 1400
BseGI	GGATG	5 cut(s)	550, 997, 1275, 1361, 1535
BseJI	GATNNNNATC	1 cut(s)	1590
BseLI	CCNNNNNNNGG	1 cut(s)	1509
BseMI	GCAATG	1 cut(s)	573
BseMII	CTCAG	6 cut(s)	32, 261, 474, 491, 1398, 1587
BseNI	ACTGG	4 cut(s)	424, 916, 993, 1342
BseRI	GAGGAG	1 cut(s)	1187
BseXI	GCAGC	1 cut(s)	1624
BsgI	GTGCAG	2 cut(s)	885, 1464
Bsh1236I	CGCG	1 cut(s)	234
BshFI	GGCC	4 cut(s)	231, 722, 1103, 1236
BshVI	ATCGAT	1 cut(s)	117
BsiHKAI	GWGCWC	2 cut(s)	247, 1010
BsiSI	CCGG	2 cut(s)	138, 723
BslFI	GGGAC	2 cut(s)	593, 1667
BslI	CCNNNNNNNGG	1 cut(s)	1509
BsmAI	GTCTC	3 cut(s)	37, 979, 1212
BsmBI	CGTCTC	1 cut(s)	1212
BsmFI	GGGAC	2 cut(s)	593, 1667
BsmI	GAATGC	3 cut(s)	450, 601, 1367
BsnI	GGCC	4 cut(s)	231, 722, 1103, 1236
Bsp1286I	GDGCHC	2 cut(s)	247, 1010
Bsp1720I	GCTNAGC	2 cut(s)	1379, 1407
Bsp19I	CCATGG	2 cut(s)	1104, 1237
BspACI	CCGC	2 cut(s)	232, 368
BspANI	GGCC	4 cut(s)	231, 722, 1103, 1236
BspCNI	CTCAG	6 cut(s)	33, 262, 475, 492, 1399, 1588
BspDI	ATCGAT	1 cut(s)	117
BspFNI	CGCG	1 cut(s)	234
BspLI	GGNNCC	1 cut(s)	681
BspMI	ACCTGC	1 cut(s)	1436
BspPI	GGATC	4 cut(s)	793, 814, 1493, 1586
BsrDI	GCAATG	1 cut(s)	573
BsrI	ACTGG	4 cut(s)	424, 916, 993, 1342
BssECI	CCNNGG	3 cut(s)	1104, 1237, 1400
BssNI	GRCGYC	1 cut(s)	1205
BssSI	CACGAG	2 cut(s)	921, 1671
BssT1I	CCWWGG	3 cut(s)	1104, 1237, 1400
Bst2BI	CACGAG	2 cut(s)	921, 1671
Bst4CI	ACNGT	1 cut(s)	973
Bst6I	CTCTTC	3 cut(s)	962, 1320, 1682
BstACI	GRCGYC	1 cut(s)	1205
BstAPI	GCANNNNNTGC	2 cut(s)	1034, 1646
BstBAI	YACGTR	1 cut(s)	1442
BstC8I	GCNNGC	2 cut(s)	6, 247
BstDEI	CTNAG	8 cut(s)	41, 270, 483, 500, 1379, 1407, 1423, 1596
BstDSI	CCRYGG	2 cut(s)	1104, 1237
BstF5I	GGATG	5 cut(s)	550, 997, 1275, 1361, 1535
BstFNI	CGCG	1 cut(s)	234
BstHHI	GCGC	1 cut(s)	1714
BstMAI	GTCTC	3 cut(s)	37, 979, 1212
BstMWI	GCNNNNNNNGC	5 cut(s)	228, 1034, 1163, 1646, 1756
BstSCI	CCNGG	1 cut(s)	136
BstSFI	CTRYAG	1 cut(s)	934
BstUI	CGCG	1 cut(s)	234
BstV1I	GCAGC	1 cut(s)	1624
BstV2I	GAAGAC	1 cut(s)	1060
BstX2I	RGATCY	2 cut(s)	952, 1429
BstYI	RGATCY	2 cut(s)	952, 1429
Bsu15I	ATCGAT	1 cut(s)	117
BsuRI	GGCC	4 cut(s)	231, 722, 1103, 1236
BsuTUI	ATCGAT	1 cut(s)	117
BtgI	CCRYGG	2 cut(s)	1104, 1237
BtsCI	GGATG	5 cut(s)	550, 997, 1275, 1361, 1535
BtsI	GCAGTG	1 cut(s)	897
BtsIMutI	CAGTG	2 cut(s)	417, 897
BveI	ACCTGC	1 cut(s)	1436
Cac8I	GCNNGC	2 cut(s)	6, 247
CfoI	GCGC	1 cut(s)	1714
Cfr13I	GGNCC	1 cut(s)	73
ClaI	ATCGAT	1 cut(s)	117
CseI	GACGC	1 cut(s)	350
CspCI	CAANNNNNGTGG	1 cut(s)	28
DdeI	CTNAG	8 cut(s)	41, 270, 483, 500, 1379, 1407, 1423, 1596
EaeI	YGGCCR	4 cut(s)	229, 720, 1101, 1234
Eam1104I	CTCTTC	3 cut(s)	962, 1320, 1682
EarI	CTCTTC	3 cut(s)	962, 1320, 1682
Eco130I	CCWWGG	3 cut(s)	1104, 1237, 1400
Eco32I	GATATC	1 cut(s)	1300
Eco47I	GGWCC	1 cut(s)	73
Eco57I	CTGAAG	1 cut(s)	943
Eco72I	CACGTG	1 cut(s)	1442
EcoRI	GAATTC	1 cut(s)	1600
EcoRV	GATATC	1 cut(s)	1300
EcoT14I	CCWWGG	3 cut(s)	1104, 1237, 1400
EcoT22I	ATGCAT	4 cut(s)	759, 774, 823, 1578
ErhI	CCWWGG	3 cut(s)	1104, 1237, 1400
Esp3I	CGTCTC	1 cut(s)	1212
FaqI	GGGAC	2 cut(s)	593, 1667
FauI	CCCGC	1 cut(s)	361
FauNDI	CATATG	1 cut(s)	823
FbaI	TGATCA	2 cut(s)	162, 207
Fnu4HI	GCNGC	3 cut(s)	232, 1638, 1757
FokI	GGATG	5 cut(s)	557, 1004, 1282, 1368, 1542
Fsp4HI	GCNGC	3 cut(s)	232, 1638, 1757
FspBI	CTAG	3 cut(s)	950, 1118, 1401
GlaI	GCGC	1 cut(s)	1713
GluI	GCNGC	3 cut(s)	232, 1638, 1757
HaeIII	GGCC	4 cut(s)	231, 722, 1103, 1236
HapII	CCGG	2 cut(s)	138, 723
HgaI	GACGC	1 cut(s)	350
HhaI	GCGC	1 cut(s)	1714
Hin1I	GRCGYC	1 cut(s)	1205
Hin6I	GCGC	1 cut(s)	1712
HinP1I	GCGC	1 cut(s)	1712
HincII	GTYRAC	1 cut(s)	1609
HindII	GTYRAC	1 cut(s)	1609
HindIII	AAGCTT	1 cut(s)	336
HinfI	GANTC	3 cut(s)	22, 100, 638
HpaI	GTTAAC	1 cut(s)	1609
HpaII	CCGG	2 cut(s)	138, 723
HphI	GGTGA	3 cut(s)	17, 685, 751
Hpy166II	GTNNAC	1 cut(s)	1609
Hpy188I	TCNGA	5 cut(s)	42, 189, 377, 574, 1212
Hpy188III	TCNNGA	7 cut(s)	32, 683, 700, 1094, 1218, 1595, 1731
Hpy8I	GTNNAC	1 cut(s)	1609
Hpy99I	CGWCG	1 cut(s)	366
HpyAV	CCTTC	9 cut(s)	38, 468, 665, 706, 736, 832, 920, 1221, 1346
HpyCH4III	ACNGT	1 cut(s)	973
HpyCH4IV	ACGT	2 cut(s)	1205, 1441
HpyF10VI	GCNNNNNNNGC	5 cut(s)	228, 1034, 1163, 1646, 1756
HpyF3I	CTNAG	8 cut(s)	41, 270, 483, 500, 1379, 1407, 1423, 1596
HpySE526I	ACGT	2 cut(s)	1205, 1441
Hsp92I	GRCGYC	1 cut(s)	1205
HspAI	GCGC	1 cut(s)	1712
Ksp22I	TGATCA	2 cut(s)	162, 207
KspAI	GTTAAC	1 cut(s)	1609
LmnI	GCTCC	3 cut(s)	1013, 1241, 1514
Lsp1109I	GCAGC	1 cut(s)	1624
LweI	GCATC	8 cut(s)	123, 535, 781, 1144, 1577, 1585, 1638, 1649
MaeI	CTAG	3 cut(s)	950, 1118, 1401
MaeII	ACGT	2 cut(s)	1205, 1441
MaeIII	GTNAC	5 cut(s)	23, 632, 929, 1478, 1551
MboII	GAAGA	7 cut(s)	109, 949, 1065, 1163, 1307, 1469, 1699
MfeI	CAATTG	1 cut(s)	1251
MflI	RGATCY	2 cut(s)	952, 1429
MhlI	GDGCHC	2 cut(s)	247, 1010
MlsI	TGGCCA	1 cut(s)	1103
MluCI	AATT	7 cut(s)	190, 203, 487, 906, 1194, 1251, 1600
MluNI	TGGCCA	1 cut(s)	1103
MlyI	GAGTC	2 cut(s)	31, 632
MmeI	TCCRAC	1 cut(s)	552
Mox20I	TGGCCA	1 cut(s)	1103
Mph1103I	ATGCAT	4 cut(s)	759, 774, 823, 1578
MscI	TGGCCA	1 cut(s)	1103
MseI	TTAA	4 cut(s)	1017, 1491, 1608, 1765
MslI	CAYNNNNRTG	3 cut(s)	156, 158, 1581
Msp20I	TGGCCA	1 cut(s)	1103
MspI	CCGG	2 cut(s)	138, 723
MspR9I	CCNGG	1 cut(s)	138
MunI	CAATTG	1 cut(s)	1251
Mva1269I	GAATGC	3 cut(s)	450, 601, 1367
MvnI	CGCG	1 cut(s)	234
MwoI	GCNNNNNNNGC	5 cut(s)	228, 1034, 1163, 1646, 1756
NciI	CCSGG	1 cut(s)	138
NcoI	CCATGG	2 cut(s)	1104, 1237
NdeI	CATATG	1 cut(s)	823
NlaIV	GGNNCC	1 cut(s)	681
NmuCI	GTSAC	4 cut(s)	23, 632, 1478, 1551
NsiI	ATGCAT	4 cut(s)	759, 774, 823, 1578
OliI	CACNNNNGTG	1 cut(s)	158
PctI	GAATGC	3 cut(s)	450, 601, 1367
PfeI	GAWTC	1 cut(s)	100
PflFI	GACNNNGTC	1 cut(s)	138
PkrI	GCNGC	3 cut(s)	233, 1639, 1758
PleI	GAGTC	2 cut(s)	30, 632
PmaCI	CACGTG	1 cut(s)	1442
PmlI	CACGTG	1 cut(s)	1442
PpsI	GAGTC	2 cut(s)	30, 632
Ppu21I	YACGTR	1 cut(s)	1442
PspCI	CACGTG	1 cut(s)	1442
PspN4I	GGNNCC	1 cut(s)	681
PspPI	GGNCC	1 cut(s)	73
PsuI	RGATCY	2 cut(s)	952, 1429
PsyI	GACNNNGTC	1 cut(s)	138
RseI	CAYNNNNRTG	3 cut(s)	156, 158, 1581
SaqAI	TTAA	4 cut(s)	1017, 1491, 1608, 1765
SatI	GCNGC	3 cut(s)	232, 1638, 1757
Sau96I	GGNCC	1 cut(s)	73
SchI	GAGTC	2 cut(s)	31, 632
ScrFI	CCNGG	1 cut(s)	138
SduI	GDGCHC	2 cut(s)	247, 1010
SfaNI	GCATC	8 cut(s)	123, 535, 781, 1144, 1577, 1585, 1638, 1649
SfcI	CTRYAG	1 cut(s)	934
SinI	GGWCC	1 cut(s)	73
SmiMI	CAYNNNNRTG	3 cut(s)	156, 158, 1581
SmlI	CTYRAG	1 cut(s)	1277
SmoI	CTYRAG	1 cut(s)	1277
Sse9I	AATT	7 cut(s)	190, 203, 487, 906, 1194, 1251, 1600
SsiI	CCGC	2 cut(s)	232, 368
SspMI	CTAG	3 cut(s)	950, 1118, 1401
StyD4I	CCNGG	1 cut(s)	136
StyI	CCWWGG	3 cut(s)	1104, 1237, 1400
TaaI	ACNGT	1 cut(s)	973
TaiI	ACGT	2 cut(s)	1208, 1444
TaqI	TCGA	3 cut(s)	117, 258, 561
TaqII	GACCGA	1 cut(s)	645
TasI	AATT	7 cut(s)	190, 203, 487, 906, 1194, 1251, 1600
TauI	GCSGC	1 cut(s)	234
TfiI	GAWTC	1 cut(s)	100
Tru1I	TTAA	4 cut(s)	1017, 1491, 1608, 1765
Tru9I	TTAA	4 cut(s)	1017, 1491, 1608, 1765
TscAI	CASTG	2 cut(s)	424, 904
TseFI	GTSAC	4 cut(s)	23, 632, 1478, 1551
TseI	GCWGC	2 cut(s)	1637, 1756
Tsp45I	GTSAC	4 cut(s)	23, 632, 1478, 1551
TspDTI	ATGAA	5 cut(s)	110, 509, 1029, 1188, 1499
TspRI	CASTG	2 cut(s)	424, 904
Tth111I	GACNNNGTC	1 cut(s)	138
VpaK11BI	GGWCC	1 cut(s)	73
XapI	RAATTY	3 cut(s)	190, 487, 1600
XmaJI	CCTAGG	1 cut(s)	1400
XspI	CTAG	3 cut(s)	950, 1118, 1401
ZraI	GACGTC	1 cut(s)	1206
Zsp2I	ATGCAT	4 cut(s)	759, 774, 823, 1578

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.