AT3G22845

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004390
Physical Location & Seq
Forward (+)
8087238 .. 8088824
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22845.1

Sequence Viewer

Length: 645 bp
ATGGAGAGACGACAAGCGAAGATCCATGTCTTTGTATTGATAGGTCTAATTCTATTGAATTCAATCAATCAAATCTCATCTCTCTCTGTAACGGTGAACGACGAAGAATGTGTCCAAGAGTACGTCCTTTACGAAGGCGACACTGTTTCCGGTAACTTCGTCGTCGTCGATCATGATATCTTCTGGGGATCCGATCATCCTGGTTTGGATTTCACGGTGACGTCACCAGCTGGGAACATTGTACAGACTTTAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCTCCAAAAAGTGGAATGTACAAATTTTGTTTCCACAATCCTTATTCTACTCCTGAAACTGTCTCCTTTTATATTCATGTTGGTCATATTCCTAATGAGCATGACTTAGCCAAAGATGAACATTTGGACCCGGTAAATGTTAAGATAGCTGAGTTGAGAGAGGCTTTGGAATCTGTTGTTGCTGAGCAGAAGTATTTGAAAGCTCGTGACACTCGTCACCGTCATACGAATGAGAGCACGAGAAAGCGAGTGATATTCTACACAGTAGGAGAGTACATTTTCCTTGCCGCAGCTAGTGGACTTCAAGTTCTCTACATCCGCAAGCTCTTCAGCAAGTCGGTAGCATACAACAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

214

Amino Acids

24.28

Weight (kDa)

6.36

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 30 - 205 5.7e-47 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 296
AciI CCGC 2 cut(s) 573, 604
AclWI GGATC 3 cut(s) 16, 183, 196
AcsI RAATTY 2 cut(s) 58, 308
AcuI CTGAAG 1 cut(s) 598
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 4 cut(s) 122, 243, 305, 560
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 4 cut(s) 58, 63, 484, 590
AjnI CCWGG 1 cut(s) 199
AloI GAACNNNNNNTCC 2 cut(s) 576, 608
AluBI AGCT 5 cut(s) 230, 434, 488, 578, 610
AluI AGCT 5 cut(s) 230, 434, 488, 578, 610
Alw21I GWGCWC 1 cut(s) 524
Alw26I GTCTC 1 cut(s) 352
AlwI GGATC 3 cut(s) 16, 183, 196
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 2 cut(s) 58, 308
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 4 cut(s) 106, 216, 229, 494
AvaII GGWCC 1 cut(s) 412
BamHI GGATCC 1 cut(s) 188
BauI CACGAG 2 cut(s) 489, 523
Bbv12I GWGCWC 1 cut(s) 524
BbvI GCAGC 1 cut(s) 587
BciT130I CCWGG 1 cut(s) 201
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 352
BfaI CTAG 1 cut(s) 579
BisI GCNGC 2 cut(s) 573, 576
BlpI GCTNAGC 1 cut(s) 468
BlsI GCNGC 2 cut(s) 574, 577
Bme1390I CCNGG 2 cut(s) 201, 416
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 3 cut(s) 190, 288, 414
BmrFI CCNGG 2 cut(s) 201, 416
BoxI GACNNNNGTC 1 cut(s) 498
BpmI CTGGAG 1 cut(s) 285
Bpu1102I GCTNAGC 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 296
BseBI CCWGG 1 cut(s) 201
BseGI GGATG 2 cut(s) 196, 600
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 426, 459
BseXI GCAGC 1 cut(s) 587
BseYI CCCAGC 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 524
BsiSI CCGG 2 cut(s) 150, 416
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 524
Bsp1407I TGTACA 2 cut(s) 241, 303
Bsp143I GATC 4 cut(s) 21, 169, 188, 193
Bsp1720I GCTNAGC 1 cut(s) 468
BspACI CCGC 2 cut(s) 573, 604
BspCNI CTCAG 2 cut(s) 427, 460
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 3 cut(s) 190, 288, 414
BspPI GGATC 3 cut(s) 16, 183, 196
BspQI GCTCTTC 1 cut(s) 617
BsrGI TGTACA 2 cut(s) 241, 303
BssMI GATC 4 cut(s) 21, 169, 188, 193
BssNI GRCGYC 1 cut(s) 221
BssSI CACGAG 2 cut(s) 489, 523
Bst2BI CACGAG 2 cut(s) 489, 523
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 6 cut(s) 94, 145, 217, 346, 506, 550
Bst6I CTCTTC 1 cut(s) 617
BstACI GRCGYC 1 cut(s) 221
BstAUI TGTACA 2 cut(s) 241, 303
BstC8I GCNNGC 1 cut(s) 608
BstDEI CTNAG 3 cut(s) 391, 435, 468
BstF5I GGATG 2 cut(s) 196, 600
BstKTI GATC 4 cut(s) 24, 172, 191, 196
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 4 cut(s) 21, 169, 188, 193
BstNI CCWGG 1 cut(s) 201
BstPAI GACNNNNGTC 1 cut(s) 498
BstSCI CCNGG 2 cut(s) 199, 414
BstV1I GCAGC 1 cut(s) 587
BstX2I RGATCY 2 cut(s) 21, 188
BstYI RGATCY 2 cut(s) 21, 188
BtsCI GGATG 2 cut(s) 196, 600
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 608
CciI TCATGA 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 412
Csp6I GTAC 4 cut(s) 121, 242, 304, 559
CviAII CATG 4 cut(s) 26, 173, 362, 386
CviJI RGCY 8 cut(s) 230, 287, 395, 434, 449, 488, 578, 610
CviKI_1 RGCY 8 cut(s) 230, 287, 395, 434, 449, 488, 578, 610
CviQI GTAC 4 cut(s) 121, 242, 304, 559
DdeI CTNAG 3 cut(s) 391, 435, 468
DpnI GATC 4 cut(s) 23, 171, 190, 195
DpnII GATC 4 cut(s) 21, 169, 188, 193
DraI TTTAAA 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Eco32I GATATC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 199
EcoRV GATATC 1 cut(s) 178
FaeI CATG 4 cut(s) 29, 176, 365, 389
FaiI YATR 8 cut(s) 27, 174, 357, 363, 372, 387, 510, 631
FatI CATG 4 cut(s) 25, 172, 361, 385
Fnu4HI GCNGC 2 cut(s) 573, 576
FokI GGATG 2 cut(s) 183, 587
Fsp4HI GCNGC 2 cut(s) 573, 576
FspBI CTAG 1 cut(s) 579
GluI GCNGC 2 cut(s) 573, 576
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 285
HapII CCGG 2 cut(s) 150, 416
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 4 cut(s) 29, 176, 365, 389
HinfI GANTC 1 cut(s) 455
HpaII CCGG 2 cut(s) 150, 416
HphI GGTGA 4 cut(s) 106, 216, 229, 494
Hpy166II GTNNAC 2 cut(s) 97, 584
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 4 cut(s) 173, 264, 338, 491
Hpy8I GTNNAC 2 cut(s) 97, 584
Hpy99I CGWCG 4 cut(s) 104, 164, 167, 170
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 6 cut(s) 94, 145, 217, 346, 506, 550
HpyCH4IV ACGT 2 cut(s) 123, 221
HpyF3I CTNAG 3 cut(s) 391, 435, 468
HpySE526I ACGT 2 cut(s) 123, 221
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 4 cut(s) 29, 176, 365, 389
Kzo9I GATC 4 cut(s) 21, 169, 188, 193
LguI GCTCTTC 1 cut(s) 617
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 9 cut(s) 163, 169, 186, 213, 216, 240, 249, 351, 429
Lsp1109I GCAGC 1 cut(s) 587
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 123, 221
MaeIII GTNAC 6 cut(s) 88, 152, 217, 222, 491, 500
MalI GATC 4 cut(s) 23, 171, 190, 195
MboI GATC 4 cut(s) 21, 169, 188, 193
MboII GAAGA 4 cut(s) 31, 116, 172, 604
MflI RGATCY 2 cut(s) 21, 188
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 3 cut(s) 48, 58, 308
MnlI CCTC 1 cut(s) 439
MseI TTAA 3 cut(s) 251, 282, 426
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 2 cut(s) 150, 416
MspR9I CCNGG 2 cut(s) 201, 416
MvaI CCWGG 1 cut(s) 201
NciI CCSGG 1 cut(s) 416
NdeII GATC 4 cut(s) 21, 169, 188, 193
NlaIII CATG 4 cut(s) 29, 176, 365, 389
NlaIV GGNNCC 3 cut(s) 190, 288, 414
NmuCI GTSAC 4 cut(s) 217, 222, 491, 500
PagI TCATGA 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 165, 496
PfeI GAWTC 1 cut(s) 455
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 2 cut(s) 574, 577
PshAI GACNNNNGTC 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 3 cut(s) 190, 288, 414
PspPI GGNCC 1 cut(s) 412
PsuI RGATCY 2 cut(s) 21, 188
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 4 cut(s) 122, 243, 305, 560
RsaNI GTAC 4 cut(s) 121, 242, 304, 559
RseI CAYNNNNRTG 1 cut(s) 513
SapI GCTCTTC 1 cut(s) 617
SaqAI TTAA 3 cut(s) 251, 282, 426
SatI GCNGC 2 cut(s) 573, 576
Sau3AI GATC 4 cut(s) 21, 169, 188, 193
Sau96I GGNCC 1 cut(s) 412
ScrFI CCNGG 2 cut(s) 201, 416
SduI GDGCHC 1 cut(s) 524
SetI ASST 8 cut(s) 46, 126, 224, 232, 436, 490, 580, 612
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 513
Sse9I AATT 3 cut(s) 48, 58, 308
SsiI CCGC 2 cut(s) 573, 604
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 199, 414
TaaI ACNGT 6 cut(s) 94, 145, 217, 346, 506, 550
TaiI ACGT 2 cut(s) 126, 224
TaqI TCGA 1 cut(s) 168
TasI AATT 3 cut(s) 48, 58, 308
TatI WGTACW 3 cut(s) 241, 303, 558
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 3 cut(s) 251, 282, 426
Tru9I TTAA 3 cut(s) 251, 282, 426
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 4 cut(s) 217, 222, 491, 500
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 4 cut(s) 217, 222, 491, 500
TspDTI ATGAA 2 cut(s) 350, 417
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 2 cut(s) 58, 308
XspI CTAG 1 cut(s) 579
ZraI GACGTC 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.