Rh4CG021100

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004390
Physical Location & Seq
Forward (+)
4236585 .. 4238872
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG021100.1

Sequence Viewer

Length: 606 bp
ATGGGGATTCTATTGGTGAGCTTTGTGGGGCGAATCGCGTCGCTATCGGTGACGGTGAACGACATGGAATGCGTGTACGAGTATGTGCTCTACGAAGACGACGTCGTTTCGGGGAACTTCGTGGTGGTCGACCATGATATCTTCTGGGCCAGCGATCATCCGGGTCTGGATTTAATTGTGACATCTCCTGGGGGTAATACAGTGCACACTTCAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCTCCAAGGAGTGGAATGTACAAGTTCTGTTTTCACAATCCCTACTCAACTCCAGAAACGGTTTCATTCAATATACATGTCGGCCATATTCCTACTGAGCACAACATTGCCAAGGATGAACATCTAGACCCTATTAATGTGAAGATAGCTGAGCTTAGAGAGGCATTGGAGTCAGTTACATTGGAACAAAAGTACTTGAAAGCACGTGATGCTCGCCATCGTCATACAAATGAGAGCACAAGAAAGCGTGTTGTGCTCTACACAGTGGGAGAGTACTTCATGCTAGTTGCTGCAAGTGCTTTACAAGTTGTATACATCCGCCGCCTCTTTAGCAAATCAATTGGATATAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

201

Amino Acids

22.71

Weight (kDa)

6.35

Isoelectric Point (pI)

27.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 17 - 193 8e-48 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 2 cut(s) 129, 558
AccII CGCG 1 cut(s) 38
AciI CCGC 2 cut(s) 565, 568
AcoI YGGCCR 1 cut(s) 328
AcvI CACGTG 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 4 cut(s) 77, 266, 440, 521
AfiI CCNNNNNNNGG 1 cut(s) 257
AflIII ACRYGT 1 cut(s) 322
AgsI TTSAA 3 cut(s) 213, 316, 445
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 3 cut(s) 21, 395, 400
AluI AGCT 3 cut(s) 21, 395, 400
Alw21I GWGCWC 5 cut(s) 90, 207, 348, 485, 504
Alw44I GTGCAC 1 cut(s) 203
AoxI GGCC 2 cut(s) 147, 328
ApaLI GTGCAC 1 cut(s) 203
ApeKI GCWGC 1 cut(s) 536
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AseI ATTAAT 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 147
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 28, 61, 67
BaeGI GKGCMC 1 cut(s) 207
BbrPI CACGTG 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 5 cut(s) 90, 207, 348, 485, 504
BbvI GCAGC 1 cut(s) 523
BccI CCATC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 189
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 2 cut(s) 371, 530
BisI GCNGC 2 cut(s) 537, 568
BlpI GCTNAGC 1 cut(s) 396
BlsI GCNGC 2 cut(s) 538, 569
BmcAI AGTACT 2 cut(s) 440, 521
Bme1390I CCNGG 2 cut(s) 162, 189
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 162, 189
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 2 cut(s) 246, 282
Bpu1102I GCTNAGC 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 452
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 188, 251, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 188, 251, 357
BseGI GGATG 3 cut(s) 157, 367, 561
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 257
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 2 cut(s) 333, 387
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 1 cut(s) 523
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 149, 330
BsiHKAI GWGCWC 5 cut(s) 90, 207, 348, 485, 504
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 257
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 2 cut(s) 149, 330
Bsp1286I GDGCHC 5 cut(s) 90, 207, 348, 485, 504
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 1 cut(s) 154
Bsp1720I GCTNAGC 1 cut(s) 396
BspACI CCGC 2 cut(s) 565, 568
BspANI GGCC 2 cut(s) 149, 330
BspCNI CTCAG 2 cut(s) 334, 388
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 264
BssECI CCNNGG 3 cut(s) 188, 251, 357
BssMI GATC 1 cut(s) 154
BssNAI GTATAC 1 cut(s) 559
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 2 cut(s) 251, 357
Bst1107I GTATAC 1 cut(s) 559
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 4 cut(s) 55, 202, 307, 511
BstACI GRCGYC 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 455
BstAUI TGTACA 1 cut(s) 264
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 151, 460
BstDEI CTNAG 3 cut(s) 342, 396, 401
BstF5I GGATG 3 cut(s) 157, 367, 561
BstFNI CGCG 1 cut(s) 38
BstKTI GATC 1 cut(s) 157
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 4 cut(s) 455, 499, 542, 576
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 160, 187
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 1 cut(s) 102
BstZ17I GTATAC 1 cut(s) 559
BsuRI GGCC 2 cut(s) 149, 330
BtsCI GGATG 3 cut(s) 157, 367, 561
BtsIMutI CAGTG 2 cut(s) 207, 516
Cac8I GCNNGC 2 cut(s) 151, 460
Cfr13I GGNCC 1 cut(s) 147
CseI GACGC 1 cut(s) 27
Csp6I GTAC 4 cut(s) 76, 265, 439, 520
CviAII CATG 4 cut(s) 64, 134, 323, 526
CviJI RGCY 6 cut(s) 21, 149, 248, 330, 395, 400
CviKI_1 RGCY 6 cut(s) 21, 149, 248, 330, 395, 400
CviQI GTAC 4 cut(s) 76, 265, 439, 520
DdeI CTNAG 3 cut(s) 342, 396, 401
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 328
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 2 cut(s) 251, 357
Eco32I GATATC 1 cut(s) 139
Eco72I CACGTG 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 187
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 251, 357
ErhI CCWWGG 2 cut(s) 251, 357
FaeI CATG 4 cut(s) 67, 137, 326, 529
FatI CATG 4 cut(s) 63, 133, 322, 525
FblI GTMKAC 2 cut(s) 129, 558
Fnu4HI GCNGC 2 cut(s) 537, 568
FokI GGATG 3 cut(s) 144, 374, 548
Fsp4HI GCNGC 2 cut(s) 537, 568
FspBI CTAG 2 cut(s) 371, 530
GluI GCNGC 2 cut(s) 537, 568
GsuI CTGGAG 2 cut(s) 246, 282
HaeIII GGCC 2 cut(s) 149, 330
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 4 cut(s) 67, 137, 326, 529
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 3 cut(s) 7, 33, 416
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 28, 61, 67
Hpy166II GTNNAC 5 cut(s) 58, 76, 130, 205, 559
Hpy188III TCNNGA 4 cut(s) 167, 225, 299, 371
Hpy8I GTNNAC 5 cut(s) 58, 76, 130, 205, 559
Hpy99I CGWCG 3 cut(s) 43, 104, 107
HpyCH4III ACNGT 4 cut(s) 55, 202, 307, 511
HpyCH4IV ACGT 2 cut(s) 102, 451
HpyCH4V TGCA 2 cut(s) 205, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 455, 499, 542, 576
HpyF3I CTNAG 3 cut(s) 342, 396, 401
HpySE526I ACGT 2 cut(s) 102, 451
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 4 cut(s) 67, 137, 326, 529
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 8 cut(s) 130, 152, 163, 174, 174, 201, 210, 312
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 371, 530
MaeII ACGT 2 cut(s) 102, 451
MaeIII GTNAC 3 cut(s) 49, 178, 421
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 3 cut(s) 107, 133, 400
MfeI CAATTG 1 cut(s) 585
MhlI GDGCHC 5 cut(s) 90, 207, 348, 485, 504
MluCI AATT 2 cut(s) 174, 585
MlyI GAGTC 1 cut(s) 425
MnlI CCTC 2 cut(s) 400, 581
MseI TTAA 3 cut(s) 173, 243, 381
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 162, 189
MunI CAATTG 1 cut(s) 585
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 4 cut(s) 455, 499, 542, 576
NciI CCSGG 1 cut(s) 162
NdeII GATC 1 cut(s) 154
NlaIII CATG 4 cut(s) 67, 137, 326, 529
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 2 cut(s) 49, 178
NspI RCATGY 1 cut(s) 326
PciI ACATGT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 126
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 7, 33
PflFI GACNNNGTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 2 cut(s) 538, 569
PleI GAGTC 1 cut(s) 424
PmaCI CACGTG 1 cut(s) 452
PmlI CACGTG 1 cut(s) 452
PpsI GAGTC 1 cut(s) 424
Ppu21I YACGTR 1 cut(s) 452
PscI ACATGT 1 cut(s) 322
PshBI ATTAAT 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 187
PspCI CACGTG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 147
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 4 cut(s) 77, 266, 440, 521
RsaNI GTAC 4 cut(s) 76, 265, 439, 520
RseI CAYNNNNRTG 1 cut(s) 474
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 3 cut(s) 173, 243, 381
SatI GCNGC 2 cut(s) 537, 568
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 1 cut(s) 147
ScaI AGTACT 2 cut(s) 440, 521
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 2 cut(s) 162, 189
SduI GDGCHC 5 cut(s) 90, 207, 348, 485, 504
SetI ASST 5 cut(s) 23, 105, 397, 402, 454
SfaNI GCATC 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 2 cut(s) 174, 585
SsiI CCGC 2 cut(s) 565, 568
SspMI CTAG 2 cut(s) 371, 530
StyD4I CCNGG 2 cut(s) 160, 187
StyI CCWWGG 2 cut(s) 251, 357
TaaI ACNGT 4 cut(s) 55, 202, 307, 511
TaiI ACGT 2 cut(s) 105, 454
TaqI TCGA 1 cut(s) 129
TasI AATT 2 cut(s) 174, 585
TatI WGTACW 3 cut(s) 264, 438, 519
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 2 cut(s) 7, 33
Tru1I TTAA 3 cut(s) 173, 243, 381
Tru9I TTAA 3 cut(s) 173, 243, 381
TscAI CASTG 2 cut(s) 207, 516
TseFI GTSAC 2 cut(s) 49, 178
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 2 cut(s) 49, 178
TspDTI ATGAA 3 cut(s) 300, 378, 514
TspRI CASTG 2 cut(s) 207, 516
Tth111I GACNNNGTC 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 257
VneI GTGCAC 1 cut(s) 203
VspI ATTAAT 1 cut(s) 381
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 1 cut(s) 326
XmiI GTMKAC 2 cut(s) 129, 558
XspI CTAG 2 cut(s) 371, 530
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 2 cut(s) 440, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.