FvH4_4g01530

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004390
Physical Location & Seq
Forward (+)
1361367 .. 1364174
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01530.t1

Sequence Viewer

Length: 654 bp
ATGAGAAAAATGGAGTCTTGGCAGAGCAATAAGGCGGTCATTTTGATGATGGGGGTGCTTTTGGTGAGCTTAGTGGGGCGAATCGAGTCGCTATCGGTGACGGTGAACGACATCGAATGCGTGTACGAGTACGTGCTCTACGAAGACGACACCGTTTCGGGGAACTTCGTTGTCGTCGACCATGATATTTTCTGGTCAAACGATCATCCGGGTCTCGATTTGATTGTGACATCTCCTGGGGGTAATACAGTGCACACTTCAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCCCCAAGGAGTGGAATGTACAAGTTCTGCTTCCACAATCCCTACTCAACTCCAGAAACGGTTTCTTTCCATATACATGTCGGCCATATTCCATCTGAGCACAACATCGCCAAGGATGAGCATCTAGACCCTATCAATGTGAAGATAGCTGAGCTTAGAGAGGCATTGGAGTCGGTTACTTTGGAGCAAAAGTACTTGAAAGCACGTGATACTCGGCATCGCCATACAAATGAGAGCACACGAAAGCGTGTTGTGTTCTACACACTTGGAGAATACTTGATGCTAGTTGCTGCAAGTGGTCTACAAATTGTATACATCCGCCGCCTCTTCAGCAAATCAATCGGATATAATCGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

218

Amino Acids

24.74

Weight (kDa)

6.59

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 33 - 208 3.2e-47 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 305
AccI GTMKAC 3 cut(s) 177, 595, 606
AciI CCGC 3 cut(s) 35, 613, 616
AcoI YGGCCR 1 cut(s) 376
AcuI CTGAAG 1 cut(s) 607
AcvI CACGTG 1 cut(s) 500
AfaI GTAC 4 cut(s) 125, 131, 314, 488
AfiI CCNNNNNNNGG 2 cut(s) 159, 305
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 261, 493
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 3 cut(s) 69, 443, 448
AluI AGCT 3 cut(s) 69, 443, 448
Alw21I GWGCWC 4 cut(s) 138, 255, 396, 533
Alw26I GTCTC 1 cut(s) 218
Alw44I GTGCAC 1 cut(s) 251
AoxI GGCC 2 cut(s) 294, 376
ApaLI GTGCAC 1 cut(s) 251
ApeKI GCWGC 1 cut(s) 584
AspS9I GGNCC 1 cut(s) 295
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 76, 109, 115
BaeGI GKGCMC 1 cut(s) 255
BbrPI CACGTG 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 4 cut(s) 138, 255, 396, 533
BbvI GCAGC 1 cut(s) 571
BccI CCATC 2 cut(s) 43, 394
BcgI CGANNNNNNTGC 4 cut(s) 447, 481, 616, 650
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 2 cut(s) 419, 578
BisI GCNGC 2 cut(s) 585, 616
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 2 cut(s) 586, 617
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 210, 237
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 2 cut(s) 210, 237
BmsI GCATC 3 cut(s) 424, 520, 564
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 294, 330
Bpu1102I GCTNAGC 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 210
BsaAI YACGTR 2 cut(s) 133, 500
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 236, 299, 405
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 305
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 236, 299, 405
BseGI GGATG 3 cut(s) 205, 415, 609
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 159, 305
BseMII CTCAG 2 cut(s) 381, 435
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 1 cut(s) 571
BshFI GGCC 2 cut(s) 296, 378
BsiHKAI GWGCWC 4 cut(s) 138, 255, 396, 533
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 159, 305
BsmAI GTCTC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 2 cut(s) 296, 378
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 4 cut(s) 138, 255, 396, 533
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 1 cut(s) 202
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 3 cut(s) 35, 613, 616
BspANI GGCC 2 cut(s) 296, 378
BspCNI CTCAG 2 cut(s) 382, 436
BspLI GGNNCC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 312
BssECI CCNNGG 3 cut(s) 236, 299, 405
BssMI GATC 1 cut(s) 202
BssNAI GTATAC 1 cut(s) 607
BssT1I CCWWGG 2 cut(s) 299, 405
Bst1107I GTATAC 1 cut(s) 607
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 4 cut(s) 103, 154, 250, 355
Bst6I CTCTTC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 312
BstBAI YACGTR 2 cut(s) 133, 500
BstDEI CTNAG 4 cut(s) 70, 390, 444, 449
BstF5I GGATG 3 cut(s) 205, 415, 609
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 624
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 208, 235
BstSLI GKGCMC 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 1 cut(s) 150
BstZ17I GTATAC 1 cut(s) 607
BsuRI GGCC 2 cut(s) 296, 378
BtgZI GCGATG 2 cut(s) 385, 497
BtsCI GGATG 3 cut(s) 205, 415, 609
BtsIMutI CAGTG 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 295
Csp6I GTAC 4 cut(s) 124, 130, 313, 487
CviAII CATG 2 cut(s) 182, 371
CviJI RGCY 5 cut(s) 69, 296, 378, 443, 448
CviKI_1 RGCY 5 cut(s) 69, 296, 378, 443, 448
CviQI GTAC 4 cut(s) 124, 130, 313, 487
DdeI CTNAG 4 cut(s) 70, 390, 444, 449
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
EciI GGCGGA 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 299, 405
Eco31I GGTCTC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 607
Eco72I CACGTG 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 299, 405
ErhI CCWWGG 2 cut(s) 299, 405
FaeI CATG 2 cut(s) 185, 374
FaiI YATR 8 cut(s) 183, 366, 368, 372, 381, 519, 607, 642
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FatI CATG 2 cut(s) 181, 370
FblI GTMKAC 3 cut(s) 177, 595, 606
Fnu4HI GCNGC 2 cut(s) 585, 616
FokI GGATG 3 cut(s) 192, 422, 596
Fsp4HI GCNGC 2 cut(s) 585, 616
FspBI CTAG 2 cut(s) 419, 578
GluI GCNGC 2 cut(s) 585, 616
GsuI CTGGAG 2 cut(s) 294, 330
HaeIII GGCC 2 cut(s) 296, 378
HapII CCGG 1 cut(s) 209
Hin1II CATG 2 cut(s) 185, 374
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 4 cut(s) 14, 81, 86, 464
HpaII CCGG 1 cut(s) 209
HphI GGTGA 3 cut(s) 76, 109, 115
Hpy166II GTNNAC 6 cut(s) 106, 124, 178, 253, 596, 607
Hpy188I TCNGA 2 cut(s) 391, 638
Hpy188III TCNNGA 4 cut(s) 215, 273, 347, 419
Hpy8I GTNNAC 6 cut(s) 106, 124, 178, 253, 596, 607
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 4 cut(s) 103, 154, 250, 355
HpyCH4IV ACGT 2 cut(s) 132, 499
HpyCH4V TGCA 2 cut(s) 253, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 624
HpyF3I CTNAG 4 cut(s) 70, 390, 444, 449
HpySE526I ACGT 2 cut(s) 132, 499
Hsp92II CATG 2 cut(s) 185, 374
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 478
LpnPI CCDG 6 cut(s) 178, 222, 222, 249, 258, 360
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 3 cut(s) 424, 520, 564
MaeI CTAG 2 cut(s) 419, 578
MaeII ACGT 2 cut(s) 132, 499
MaeIII GTNAC 3 cut(s) 97, 226, 469
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 3 cut(s) 155, 448, 613
MhlI GDGCHC 4 cut(s) 138, 255, 396, 533
MluCI AATT 1 cut(s) 600
MlyI GAGTC 3 cut(s) 23, 95, 473
MnlI CCTC 2 cut(s) 448, 629
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 3 cut(s) 44, 369, 522
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 210, 237
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 624
NciI CCSGG 1 cut(s) 210
NdeII GATC 1 cut(s) 202
NlaIII CATG 2 cut(s) 185, 374
NlaIV GGNNCC 1 cut(s) 297
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 97, 226
NspI RCATGY 1 cut(s) 374
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 174
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 81
PflMI CCANNNNNTGG 1 cut(s) 305
PkrI GCNGC 2 cut(s) 586, 617
PleI GAGTC 3 cut(s) 22, 94, 472
PmaCI CACGTG 1 cut(s) 500
PmlI CACGTG 1 cut(s) 500
PpsI GAGTC 3 cut(s) 22, 94, 472
Ppu21I YACGTR 2 cut(s) 133, 500
PscI ACATGT 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 235
PspCI CACGTG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 295
RsaI GTAC 4 cut(s) 125, 131, 314, 488
RsaNI GTAC 4 cut(s) 124, 130, 313, 487
RseI CAYNNNNRTG 3 cut(s) 44, 369, 522
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 2 cut(s) 585, 616
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 295
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 3 cut(s) 23, 95, 473
ScrFI CCNGG 2 cut(s) 210, 237
SduI GDGCHC 4 cut(s) 138, 255, 396, 533
SetI ASST 5 cut(s) 71, 135, 445, 450, 502
SfaNI GCATC 3 cut(s) 424, 520, 564
SmiMI CAYNNNNRTG 3 cut(s) 44, 369, 522
Sse9I AATT 1 cut(s) 600
SsiI CCGC 3 cut(s) 35, 613, 616
SspMI CTAG 2 cut(s) 419, 578
StyD4I CCNGG 2 cut(s) 208, 235
StyI CCWWGG 2 cut(s) 299, 405
TaaI ACNGT 4 cut(s) 103, 154, 250, 355
TaiI ACGT 2 cut(s) 135, 502
TaqI TCGA 4 cut(s) 84, 114, 177, 216
TasI AATT 1 cut(s) 600
TatI WGTACW 2 cut(s) 312, 486
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 1 cut(s) 81
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 2 cut(s) 97, 226
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 2 cut(s) 97, 226
TspRI CASTG 1 cut(s) 255
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 251
XbaI TCTAGA 1 cut(s) 418
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 3 cut(s) 177, 595, 606
XspI CTAG 2 cut(s) 419, 578
ZrmI AGTACT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.