MD13G1200800.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004390
Physical Location & Seq
Forward (+)
17895973 .. 17899230
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1200800.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGAGGGAAGGGCGAGGGGTTTGACGCTGATTGGCCTTCTTTTGTTGAGCTTCGTCGGAAAGATCGCATCGCTATCGGTGACAGTGAACGACGTCGAATGCGTGTACGAGTACGTGCTTTACGAAGGCGATACCGTTTCGGGCAACTTCGTGGTCGTCGATCACGATATTTTCTGGGGCGCCGATCATCCCGGCCTCGATTTAATCGTGACATCTCCTGGGGATAATACGGTGCACACTTCGAAGGGAACATCTGGTGATAAGTTTGAGTTTAAAGCCCCAAGAAGCGGAATGTACAAATTTTGTTTTCACAATCCTTACTCAACGCCAGAGACTGTTTCTTTTAACATACATGTTGGTCATATTCCCAACGAGCACAATATTGCCAAAGATGAACATCTAGACCCAATAAATGTTAAAATTGCTGAGCTTAGAGAAGCATTGGAGTCAGTTACATTAGAGCAGAAGTACTTGAAAGCACGTGATGTTCGACATCGTCATACAAACGAGAGCACAAGGAGGCGGGTTGTGTTGTATACAGTGGCAGAGTATCTTGCGCTGGCTGCTGCGAGTGCTTTGCAAGTCGTTTACATTCGCAAACTTTTCAGCAAATCGGTTGCATACAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

212

Amino Acids

23.64

Weight (kDa)

6.55

Isoelectric Point (pI)

24.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 27 - 202 1.1e-46 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 536
AciI CCGC 2 cut(s) 288, 523
AcsI RAATTY 1 cut(s) 299
AcvI CACGTG 1 cut(s) 482
AcyI GRCGYC 2 cut(s) 93, 180
AfaI GTAC 4 cut(s) 107, 113, 296, 470
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 1 cut(s) 475
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 2 cut(s) 51, 430
AluI AGCT 2 cut(s) 51, 430
Alw21I GWGCWC 3 cut(s) 237, 378, 515
Alw26I GTCTC 1 cut(s) 326
Alw44I GTGCAC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 2 cut(s) 34, 193
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 563, 566
ApoI RAATTY 1 cut(s) 299
AspLEI GCGC 2 cut(s) 182, 559
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 91, 269
AsuII TTCGAA 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 237
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 482
Bbv12I GWGCWC 3 cut(s) 237, 378, 515
BbvI GCAGC 2 cut(s) 550, 553
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 559, 593
BciT130I CCWGG 1 cut(s) 219
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 1 cut(s) 401
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 2 cut(s) 564, 567
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 2 cut(s) 565, 568
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 2 cut(s) 192, 219
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 192, 219
BmsI GCATC 1 cut(s) 77
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 192
BsaAI YACGTR 2 cut(s) 115, 482
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 2 cut(s) 93, 180
BsaJI CCNNGG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 187
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 550, 553
BshFI GGCC 2 cut(s) 36, 195
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 3 cut(s) 237, 378, 515
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 2 cut(s) 36, 195
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 237, 378, 515
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 3 cut(s) 63, 160, 184
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 2 cut(s) 288, 523
BspANI GGCC 2 cut(s) 36, 195
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 179
BsrGI TGTACA 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 3 cut(s) 63, 160, 184
BssNAI GTATAC 1 cut(s) 537
BssNI GRCGYC 2 cut(s) 93, 180
Bst1107I GTATAC 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 5 cut(s) 85, 136, 232, 337, 541
BstACI GRCGYC 2 cut(s) 93, 180
BstAUI TGTACA 1 cut(s) 294
BstBAI YACGTR 2 cut(s) 115, 482
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 2 cut(s) 426, 431
BstF5I GGATG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 559
BstKTI GATC 3 cut(s) 66, 163, 187
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 3 cut(s) 63, 160, 184
BstMWI GCNNNNNNNGC 2 cut(s) 563, 572
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 190, 217
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 550, 553
BstZ17I GTATAC 1 cut(s) 537
BsuRI GGCC 2 cut(s) 36, 195
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 90, 546
Cac8I GCNNGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 2 cut(s) 182, 559
CseI GACGC 1 cut(s) 34
Csp6I GTAC 4 cut(s) 106, 112, 295, 469
CviAII CATG 1 cut(s) 353
CviJI RGCY 6 cut(s) 36, 51, 195, 278, 430, 563
CviKI_1 RGCY 6 cut(s) 36, 51, 195, 278, 430, 563
CviQI GTAC 4 cut(s) 106, 112, 295, 469
DdeI CTNAG 2 cut(s) 426, 431
DinI GGCGCC 1 cut(s) 181
DpnI GATC 3 cut(s) 65, 162, 186
DpnII GATC 3 cut(s) 63, 160, 184
DraI TTTAAA 1 cut(s) 274
Eco72I CACGTG 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 217
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
FaeI CATG 1 cut(s) 356
FaiI YATR 6 cut(s) 350, 354, 363, 501, 537, 622
FatI CATG 1 cut(s) 352
FauI CCCGC 1 cut(s) 516
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 2 cut(s) 564, 567
FokI GGATG 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 564, 567
FspBI CTAG 1 cut(s) 401
GlaI GCGC 2 cut(s) 181, 558
GluI GCNGC 2 cut(s) 564, 567
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 36, 195
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 34
HhaI GCGC 2 cut(s) 182, 559
Hin1I GRCGYC 2 cut(s) 93, 180
Hin1II CATG 1 cut(s) 356
Hin6I GCGC 2 cut(s) 180, 557
HinP1I GCGC 2 cut(s) 180, 557
HinfI GANTC 1 cut(s) 446
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 91, 269
Hpy166II GTNNAC 5 cut(s) 88, 106, 235, 537, 589
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 3 cut(s) 164, 208, 401
Hpy8I GTNNAC 5 cut(s) 88, 106, 235, 537, 589
Hpy99I CGWCG 4 cut(s) 59, 95, 98, 161
HpyAV CCTTC 4 cut(s) 3, 47, 119, 238
HpyCH4III ACNGT 5 cut(s) 85, 136, 232, 337, 541
HpyCH4IV ACGT 3 cut(s) 93, 114, 481
HpyCH4V TGCA 3 cut(s) 235, 580, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 572
HpyF3I CTNAG 2 cut(s) 426, 431
HpySE526I ACGT 3 cut(s) 93, 114, 481
Hsp92I GRCGYC 2 cut(s) 93, 180
Hsp92II CATG 1 cut(s) 356
HspAI GCGC 2 cut(s) 180, 557
KasI GGCGCC 1 cut(s) 179
Kzo9I GATC 3 cut(s) 63, 160, 184
LpnPI CCDG 7 cut(s) 160, 204, 205, 231, 240, 342, 545
Lsp1109I GCAGC 2 cut(s) 550, 553
LweI GCATC 1 cut(s) 77
MaeI CTAG 1 cut(s) 401
MaeII ACGT 3 cut(s) 93, 114, 481
MaeIII GTNAC 3 cut(s) 79, 208, 451
MalI GATC 3 cut(s) 65, 162, 186
MboI GATC 3 cut(s) 63, 160, 184
MhlI GDGCHC 3 cut(s) 237, 378, 515
MluCI AATT 2 cut(s) 299, 420
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 3 cut(s) 9, 206, 513
MseI TTAA 4 cut(s) 203, 273, 345, 417
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 192, 219
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 563, 572
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 192
NdeII GATC 3 cut(s) 63, 160, 184
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 79, 208
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 242
PciI ACATGT 1 cut(s) 352
PcsI WCGNNNNNNNCGW 6 cut(s) 99, 120, 156, 162, 204, 487
PctI GAATGC 1 cut(s) 104
PflFI GACNNNGTC 1 cut(s) 495
PkrI GCNGC 2 cut(s) 565, 568
PleI GAGTC 1 cut(s) 454
PluTI GGCGCC 1 cut(s) 183
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
PpsI GAGTC 1 cut(s) 454
Ppu21I YACGTR 2 cut(s) 115, 482
PscI ACATGT 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 217
PspCI CACGTG 1 cut(s) 482
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 495
RsaI GTAC 4 cut(s) 107, 113, 296, 470
RsaNI GTAC 4 cut(s) 106, 112, 295, 469
SaqAI TTAA 4 cut(s) 203, 273, 345, 417
SatI GCNGC 2 cut(s) 564, 567
Sau3AI GATC 3 cut(s) 63, 160, 184
ScaI AGTACT 1 cut(s) 470
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 2 cut(s) 192, 219
SduI GDGCHC 3 cut(s) 237, 378, 515
SetI ASST 5 cut(s) 53, 96, 117, 432, 484
SfaNI GCATC 1 cut(s) 77
SfoI GGCGCC 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 242
Sse9I AATT 2 cut(s) 299, 420
SsiI CCGC 2 cut(s) 288, 523
SspDI GGCGCC 1 cut(s) 179
SspI AATATT 1 cut(s) 382
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 190, 217
TaaI ACNGT 5 cut(s) 85, 136, 232, 337, 541
TaiI ACGT 3 cut(s) 96, 117, 484
TaqI TCGA 5 cut(s) 96, 159, 198, 242, 490
TasI AATT 2 cut(s) 299, 420
TatI WGTACW 2 cut(s) 294, 468
Tru1I TTAA 4 cut(s) 203, 273, 345, 417
Tru9I TTAA 4 cut(s) 203, 273, 345, 417
TscAI CASTG 2 cut(s) 90, 546
TseFI GTSAC 2 cut(s) 79, 208
TseI GCWGC 2 cut(s) 563, 566
Tsp45I GTSAC 2 cut(s) 79, 208
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 2 cut(s) 90, 546
Tth111I GACNNNGTC 1 cut(s) 495
VneI GTGCAC 1 cut(s) 233
XapI RAATTY 1 cut(s) 299
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 1 cut(s) 356
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 1 cut(s) 401
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.