Rroxscaffold_5G00334640

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004390
Physical Location & Seq
Forward (+)
1894872 .. 1897577
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334640.1

Sequence Viewer

Length: 642 bp
ATGGAGTCTTGGCAGAGCAGAAAAGGCGTGATCTTGATGGGGATTCTATTGGTGAGCTTTGTGGGGCGAATCGCGTCGCTATCGGTGACGGTGAACGACATGGAATGCGTGTACGAGTATGTGCTCTACGAAGACGACGTCGTTTCGGGGAACTTCGTGGTGGTCGACCATGATATCTTCTGGGCCAGCGATCATCCGGGTCTCGATTTAATTGTGACATCTCCTGGGGGTAATACAGTGCACACTTCAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCCCCAAGGAGTGGAATGTACAAGTTCTGTTTTCACAATCCCTACTCAACTCCAGAAACGGTTTCATTCAATATACATGTCGGCCATATTCCTACTGAGCACAACATTGCCAAGGATGAACATCTAGACCCTATTAATGTGAAGATAGCTGAGCTTAGAGAGGCATTGGAGTCAGTTACATTGGAACAAAAGTACTTGAAAGCACGTGATGCTCGCCATCGTCATACAAATGAGAGCACAAGAAAGCGTGTTGTGCTCTACACAGTGGGAGAGTACATCATGCTAGTTGCTGCAAGTGCTTTACAAGTTGTATACATCCGCCGCCTCTTTAGCAAATCAATTGGATATAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

213

Amino Acids

24.09

Weight (kDa)

6.55

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 29 - 205 7.4e-48 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 2 cut(s) 165, 594
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 601, 604
AcoI YGGCCR 1 cut(s) 364
AcvI CACGTG 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 4 cut(s) 113, 302, 476, 557
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 3 cut(s) 249, 352, 481
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 3 cut(s) 57, 431, 436
AluI AGCT 3 cut(s) 57, 431, 436
Alw21I GWGCWC 5 cut(s) 126, 243, 384, 521, 540
Alw26I GTCTC 1 cut(s) 206
Alw44I GTGCAC 1 cut(s) 239
AoxI GGCC 3 cut(s) 183, 282, 364
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
AseI ATTAAT 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 183, 283
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 3 cut(s) 64, 97, 103
BaeGI GKGCMC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 5 cut(s) 126, 243, 384, 521, 540
BbvI GCAGC 1 cut(s) 559
BccI CCATC 2 cut(s) 31, 507
BciT130I CCWGG 1 cut(s) 225
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 407, 566
BisI GCNGC 2 cut(s) 573, 604
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 2 cut(s) 574, 605
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 2 cut(s) 198, 225
BmgT120I GGNCC 2 cut(s) 183, 283
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 198, 225
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 2 cut(s) 282, 318
Bpu1102I GCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 198
BsaAI YACGTR 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 224, 287, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 387
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 224, 287, 393
BseGI GGATG 3 cut(s) 193, 403, 597
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 369, 423
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 1 cut(s) 559
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 3 cut(s) 185, 284, 366
BsiHKAI GWGCWC 5 cut(s) 126, 243, 384, 521, 540
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 3 cut(s) 185, 284, 366
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 5 cut(s) 126, 243, 384, 521, 540
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 30, 190
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 601, 604
BspANI GGCC 3 cut(s) 185, 284, 366
BspCNI CTCAG 2 cut(s) 370, 424
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 224, 287, 393
BssMI GATC 2 cut(s) 30, 190
BssNAI GTATAC 1 cut(s) 595
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 287, 393
Bst1107I GTATAC 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 4 cut(s) 91, 238, 343, 547
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 187, 496
BstDEI CTNAG 3 cut(s) 378, 432, 437
BstF5I GGATG 3 cut(s) 193, 403, 597
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 33, 193
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 2 cut(s) 30, 190
BstMWI GCNNNNNNNGC 5 cut(s) 24, 491, 535, 578, 612
BstNI CCWGG 1 cut(s) 225
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 196, 223
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 1 cut(s) 138
BstZ17I GTATAC 1 cut(s) 595
BsuRI GGCC 3 cut(s) 185, 284, 366
BtsCI GGATG 3 cut(s) 193, 403, 597
BtsIMutI CAGTG 2 cut(s) 243, 552
Cac8I GCNNGC 2 cut(s) 187, 496
Cfr13I GGNCC 2 cut(s) 183, 283
CseI GACGC 1 cut(s) 63
Csp6I GTAC 4 cut(s) 112, 301, 475, 556
CviAII CATG 4 cut(s) 100, 170, 359, 562
CviJI RGCY 6 cut(s) 57, 185, 284, 366, 431, 436
CviKI_1 RGCY 6 cut(s) 57, 185, 284, 366, 431, 436
CviQI GTAC 4 cut(s) 112, 301, 475, 556
DdeI CTNAG 3 cut(s) 378, 432, 437
DpnI GATC 2 cut(s) 32, 192
DpnII GATC 2 cut(s) 30, 190
EaeI YGGCCR 1 cut(s) 364
EciI GGCGGA 1 cut(s) 590
Eco130I CCWWGG 2 cut(s) 287, 393
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 175
Eco72I CACGTG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 287, 393
ErhI CCWWGG 2 cut(s) 287, 393
FaeI CATG 4 cut(s) 103, 173, 362, 565
FatI CATG 4 cut(s) 99, 169, 358, 561
FblI GTMKAC 2 cut(s) 165, 594
Fnu4HI GCNGC 2 cut(s) 573, 604
FokI GGATG 3 cut(s) 180, 410, 584
Fsp4HI GCNGC 2 cut(s) 573, 604
FspBI CTAG 2 cut(s) 407, 566
GluI GCNGC 2 cut(s) 573, 604
GsuI CTGGAG 2 cut(s) 282, 318
HaeIII GGCC 3 cut(s) 185, 284, 366
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 103, 173, 362, 565
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 4 cut(s) 5, 43, 69, 452
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 64, 97, 103
Hpy166II GTNNAC 5 cut(s) 94, 112, 166, 241, 595
Hpy188III TCNNGA 5 cut(s) 34, 203, 261, 335, 407
Hpy8I GTNNAC 5 cut(s) 94, 112, 166, 241, 595
Hpy99I CGWCG 3 cut(s) 79, 140, 143
HpyCH4III ACNGT 4 cut(s) 91, 238, 343, 547
HpyCH4IV ACGT 2 cut(s) 138, 487
HpyCH4V TGCA 2 cut(s) 241, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 491, 535, 578, 612
HpyF3I CTNAG 3 cut(s) 378, 432, 437
HpySE526I ACGT 2 cut(s) 138, 487
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 103, 173, 362, 565
Kzo9I GATC 2 cut(s) 30, 190
LpnPI CCDG 7 cut(s) 166, 199, 210, 210, 237, 246, 348
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 1 cut(s) 481
MaeI CTAG 2 cut(s) 407, 566
MaeII ACGT 2 cut(s) 138, 487
MaeIII GTNAC 3 cut(s) 85, 214, 457
MalI GATC 2 cut(s) 32, 192
MboI GATC 2 cut(s) 30, 190
MboII GAAGA 3 cut(s) 143, 169, 436
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 5 cut(s) 126, 243, 384, 521, 540
MluCI AATT 2 cut(s) 210, 621
MlyI GAGTC 2 cut(s) 14, 461
MnlI CCTC 2 cut(s) 436, 617
MseI TTAA 3 cut(s) 209, 279, 417
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 198, 225
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 24, 491, 535, 578, 612
NciI CCSGG 1 cut(s) 198
NdeII GATC 2 cut(s) 30, 190
NlaIII CATG 4 cut(s) 103, 173, 362, 565
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 2 cut(s) 85, 214
NspI RCATGY 1 cut(s) 362
PciI ACATGT 1 cut(s) 358
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 162
PctI GAATGC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 43, 69
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 2 cut(s) 574, 605
PleI GAGTC 2 cut(s) 13, 460
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 2 cut(s) 13, 460
Ppu21I YACGTR 1 cut(s) 488
PscI ACATGT 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 223
PspCI CACGTG 1 cut(s) 488
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 2 cut(s) 183, 283
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 4 cut(s) 113, 302, 476, 557
RsaNI GTAC 4 cut(s) 112, 301, 475, 556
RseI CAYNNNNRTG 1 cut(s) 510
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 209, 279, 417
SatI GCNGC 2 cut(s) 573, 604
Sau3AI GATC 2 cut(s) 30, 190
Sau96I GGNCC 2 cut(s) 183, 283
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 2 cut(s) 14, 461
ScrFI CCNGG 2 cut(s) 198, 225
SduI GDGCHC 5 cut(s) 126, 243, 384, 521, 540
SetI ASST 5 cut(s) 59, 141, 433, 438, 490
SfaNI GCATC 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 510
Sse9I AATT 2 cut(s) 210, 621
SsiI CCGC 2 cut(s) 601, 604
SspMI CTAG 2 cut(s) 407, 566
StyD4I CCNGG 2 cut(s) 196, 223
StyI CCWWGG 2 cut(s) 287, 393
TaaI ACNGT 4 cut(s) 91, 238, 343, 547
TaiI ACGT 2 cut(s) 141, 490
TaqI TCGA 2 cut(s) 165, 204
TasI AATT 2 cut(s) 210, 621
TatI WGTACW 3 cut(s) 300, 474, 555
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 2 cut(s) 43, 69
Tru1I TTAA 3 cut(s) 209, 279, 417
Tru9I TTAA 3 cut(s) 209, 279, 417
TscAI CASTG 2 cut(s) 243, 552
TseFI GTSAC 2 cut(s) 85, 214
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 2 cut(s) 85, 214
TspDTI ATGAA 2 cut(s) 336, 414
TspRI CASTG 2 cut(s) 243, 552
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 239
VspI ATTAAT 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 1 cut(s) 362
XmiI GTMKAC 2 cut(s) 165, 594
XspI CTAG 2 cut(s) 407, 566
ZraI GACGTC 1 cut(s) 139
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.