RLG00000010081

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004390
Physical Location & Seq
Reverse (-)
62794762 .. 62797448
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010081

Sequence Viewer

Length: 543 bp
ATGGAGTCTTGGCAGAGCAGAAAAGGCGTGATCTTGATGGGGATTCTATTGGTGAGCTTTGTGGGGCGAATTGCGTCGCTATCGGTGACGGTGAACGACATGGAATGCGTGTACGAGTATGTGCTCTACGAAGACGACGTCGTTTCGGGGAACTTCGTGGTGGTCGACCATGATATCTTCTGGGCCAGCGATCATCCGGGTATCGATTTAATTGTAACATCTCCTGGGGGTAATACAGTGCACACTTCAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCCCCAAGGAGTGGAATGTACAAGTTCTGTTTTCACAATCCCTACTCAACTCCAGAAACGGTTTCATTCAATATACATGTCGGCCATATTCCTACTGAGCACAACATTGCCAAGGATGAACATCTAGACCCTATTAATGTGAAGATAGCTGAGCTTAGAGAGGCATTGGAGTCAGTTACATTGGAACAAAAGTACTTGAAAGCACGTGATGCTCGCCATCGTCATAGTACGTTTTTCTTTCCCTTTTTCAATGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

181

Amino Acids

20.42

Weight (kDa)

5.63

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 29 - 169 2.1e-33 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 364
AcvI CACGTG 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 4 cut(s) 113, 302, 476, 511
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 4 cut(s) 249, 352, 481, 532
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 3 cut(s) 57, 431, 436
AluI AGCT 3 cut(s) 57, 431, 436
Alw21I GWGCWC 3 cut(s) 126, 243, 384
Alw44I GTGCAC 1 cut(s) 239
AoxI GGCC 3 cut(s) 183, 282, 364
ApaLI GTGCAC 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
AseI ATTAAT 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 183, 283
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 3 cut(s) 64, 97, 103
BaeGI GKGCMC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 3 cut(s) 126, 243, 384
BccI CCATC 2 cut(s) 31, 507
BciT130I CCWGG 1 cut(s) 225
BcnI CCSGG 1 cut(s) 198
BfaI CTAG 1 cut(s) 407
BlpI GCTNAGC 1 cut(s) 432
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 2 cut(s) 198, 225
BmgT120I GGNCC 2 cut(s) 183, 283
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 2 cut(s) 198, 225
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 2 cut(s) 282, 318
Bpu1102I GCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 198
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 224, 287, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 387
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 225
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 224, 287, 393
BseGI GGATG 2 cut(s) 193, 403
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 369, 423
BseSI GKGCMC 1 cut(s) 243
BshFI GGCC 3 cut(s) 185, 284, 366
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 126, 243, 384
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 293
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 3 cut(s) 185, 284, 366
Bsp1286I GDGCHC 3 cut(s) 126, 243, 384
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 30, 190
Bsp1720I GCTNAGC 1 cut(s) 432
BspANI GGCC 3 cut(s) 185, 284, 366
BspCNI CTCAG 2 cut(s) 370, 424
BspDI ATCGAT 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 224, 287, 393
BssMI GATC 2 cut(s) 30, 190
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 287, 393
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 91, 238, 343
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 187, 496
BstDEI CTNAG 3 cut(s) 378, 432, 437
BstF5I GGATG 2 cut(s) 193, 403
BstKTI GATC 2 cut(s) 33, 193
BstMBI GATC 2 cut(s) 30, 190
BstMWI GCNNNNNNNGC 2 cut(s) 24, 491
BstNI CCWGG 1 cut(s) 225
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 196, 223
BstSLI GKGCMC 1 cut(s) 243
BstV2I GAAGAC 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 3 cut(s) 185, 284, 366
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 2 cut(s) 193, 403
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 187, 496
Cfr13I GGNCC 2 cut(s) 183, 283
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 1 cut(s) 63
Csp6I GTAC 4 cut(s) 112, 301, 475, 510
CviAII CATG 3 cut(s) 100, 170, 359
CviJI RGCY 6 cut(s) 57, 185, 284, 366, 431, 436
CviKI_1 RGCY 6 cut(s) 57, 185, 284, 366, 431, 436
CviQI GTAC 4 cut(s) 112, 301, 475, 510
DdeI CTNAG 3 cut(s) 378, 432, 437
DpnI GATC 2 cut(s) 32, 192
DpnII GATC 2 cut(s) 30, 190
EaeI YGGCCR 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 287, 393
Eco32I GATATC 1 cut(s) 175
Eco72I CACGTG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 287, 393
ErhI CCWWGG 2 cut(s) 287, 393
FaeI CATG 3 cut(s) 103, 173, 362
FaiI YATR 7 cut(s) 101, 120, 171, 356, 360, 369, 507
FatI CATG 3 cut(s) 99, 169, 358
FblI GTMKAC 1 cut(s) 165
FokI GGATG 2 cut(s) 180, 410
FspBI CTAG 1 cut(s) 407
GsuI CTGGAG 2 cut(s) 282, 318
HaeIII GGCC 3 cut(s) 185, 284, 366
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 3 cut(s) 103, 173, 362
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 3 cut(s) 5, 43, 452
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 64, 97, 103
Hpy166II GTNNAC 4 cut(s) 94, 112, 166, 241
Hpy188III TCNNGA 4 cut(s) 34, 261, 335, 407
Hpy8I GTNNAC 4 cut(s) 94, 112, 166, 241
Hpy99I CGWCG 3 cut(s) 79, 140, 143
HpyCH4III ACNGT 3 cut(s) 91, 238, 343
HpyCH4IV ACGT 3 cut(s) 138, 487, 512
HpyCH4V TGCA 1 cut(s) 241
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 491
HpyF3I CTNAG 3 cut(s) 378, 432, 437
HpySE526I ACGT 3 cut(s) 138, 487, 512
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 3 cut(s) 103, 173, 362
Kzo9I GATC 2 cut(s) 30, 190
LpnPI CCDG 7 cut(s) 166, 199, 210, 210, 237, 246, 348
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 407
MaeII ACGT 3 cut(s) 138, 487, 512
MaeIII GTNAC 3 cut(s) 85, 214, 457
MalI GATC 2 cut(s) 32, 192
MboI GATC 2 cut(s) 30, 190
MboII GAAGA 3 cut(s) 143, 169, 436
MhlI GDGCHC 3 cut(s) 126, 243, 384
MluCI AATT 2 cut(s) 69, 210
MlyI GAGTC 2 cut(s) 14, 461
MnlI CCTC 1 cut(s) 436
MseI TTAA 3 cut(s) 209, 279, 417
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 198, 225
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 24, 491
NciI CCSGG 1 cut(s) 198
NdeII GATC 2 cut(s) 30, 190
NlaIII CATG 3 cut(s) 103, 173, 362
NlaIV GGNNCC 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 1 cut(s) 362
PciI ACATGT 1 cut(s) 358
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 162
PctI GAATGC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 43
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 293
PleI GAGTC 2 cut(s) 13, 460
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 2 cut(s) 13, 460
Ppu21I YACGTR 1 cut(s) 488
PscI ACATGT 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 223
PspCI CACGTG 1 cut(s) 488
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 285
PspPI GGNCC 2 cut(s) 183, 283
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 4 cut(s) 113, 302, 476, 511
RsaNI GTAC 4 cut(s) 112, 301, 475, 510
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 209, 279, 417
Sau3AI GATC 2 cut(s) 30, 190
Sau96I GGNCC 2 cut(s) 183, 283
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 2 cut(s) 14, 461
ScrFI CCNGG 2 cut(s) 198, 225
SduI GDGCHC 3 cut(s) 126, 243, 384
SetI ASST 6 cut(s) 59, 141, 433, 438, 490, 515
SfaNI GCATC 1 cut(s) 481
Sse9I AATT 2 cut(s) 69, 210
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 196, 223
StyI CCWWGG 2 cut(s) 287, 393
TaaI ACNGT 3 cut(s) 91, 238, 343
TaiI ACGT 3 cut(s) 141, 490, 515
TaqI TCGA 2 cut(s) 165, 204
TasI AATT 2 cut(s) 69, 210
TatI WGTACW 2 cut(s) 300, 474
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 3 cut(s) 209, 279, 417
Tru9I TTAA 3 cut(s) 209, 279, 417
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 336, 414
TspRI CASTG 1 cut(s) 243
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 239
VspI ATTAAT 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 407
ZraI GACGTC 1 cut(s) 139
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.