FvH4_2g22930

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004390
Physical Location & Seq
Reverse (-)
18822601 .. 18824580
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22930.t1

Sequence Viewer

Length: 654 bp
ATGGAGACCAGGAAGAGATTTGTGTGTGCTAGATTTTACGTAGTGTGCAGTTGTATGTTGTGGCTTATCGGCAATAGGAATGTTGCGGCTCTTTCGATCACTGTGACTGAATTGGAATGCGTCTCGGAGCAAGTTTTGTACGAGGGAGACACCATTTCTGGGAATTTTGTTGCCATTGATCATGATATCTTCTGGAGCCATGATCATCCCGGTATCGAATTCACCGTGACATCTCCGGGAGGCAGTACAGTATTTTCTCTCAAAGGAACATCCGGCGATAAGTTTGAACTCAAGGCGCCACAAAAAGGAATCTACAAGTTCTGTTTCCGCAATCCGTCTTCGTCTCCGGAGACTGTATCTTTCTACATACATGTTGGTCACATTCCAAATGAGCTTGACTTGGCCCAAGATGAACATATGGACCCTCTAAACATTAAGATTGCAGAGCTGAGGGAGGCATTGGAGTCTATCACAGCAGAGCAGAAGTACCTTAAAGCCCGGGATGCGAGGCATCGATACACAAATGTTGGCACCAAGAAACGTGTTATATACTTTACACTAGCAGAGTACCTTCTCTTTGCTGTTGCAAGCGCTCTTCAAGTGGTGTACATCCGTAATCTGTTCAGCAAGACAGTTGGTTACAACAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

218

Amino Acids

24.6

Weight (kDa)

7.04

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 36 - 208 2.9e-41 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 295, 530
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 2 cut(s) 86, 328
AcsI RAATTY 2 cut(s) 163, 218
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 5 cut(s) 140, 247, 488, 569, 608
AfeI AGCGCT 1 cut(s) 592
AfiI CCNNNNNNNGG 2 cut(s) 159, 305
AflIII ACRYGT 2 cut(s) 370, 541
AgsI TTSAA 2 cut(s) 287, 599
AjnI CCWGG 1 cut(s) 8
AluBI AGCT 2 cut(s) 394, 448
AluI AGCT 2 cut(s) 394, 448
Alw26I GTCTC 4 cut(s) 127, 141, 344, 348
AlwNI CAGNNNCTG 1 cut(s) 651
Ama87I CYCGRG 1 cut(s) 498
Aor13HI TCCGGA 1 cut(s) 346
Aor51HI AGCGCT 1 cut(s) 592
AoxI GGCC 1 cut(s) 402
ApoI RAATTY 2 cut(s) 163, 218
AspLEI GCGC 2 cut(s) 298, 593
AspS9I GGNCC 2 cut(s) 403, 421
AsuC2I CCSGG 4 cut(s) 210, 237, 499, 500
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 1 cut(s) 421
BanI GGYRCC 2 cut(s) 295, 530
BbsI GAAGAC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 10
BclI TGATCA 2 cut(s) 178, 202
BcnI CCSGG 4 cut(s) 210, 237, 499, 500
BcoDI GTCTC 4 cut(s) 127, 141, 344, 348
BfaI CTAG 2 cut(s) 30, 560
BfoI RGCGCY 2 cut(s) 299, 594
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 5 cut(s) 10, 210, 237, 499, 500
Bme18I GGWCC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 403, 421
BmiI GGNNCC 4 cut(s) 197, 297, 423, 532
BmrFI CCNGG 5 cut(s) 10, 210, 237, 499, 500
BmsI GCATC 2 cut(s) 493, 520
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 4 cut(s) 210, 237, 499, 500
Bsa29I ATCGAT 1 cut(s) 514
BsaAI YACGTR 1 cut(s) 40
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 498
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 305
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 10
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 4 cut(s) 205, 269, 508, 609
BseLI CCNNNNNNNGG 2 cut(s) 159, 305
BseMII CTCAG 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 67
BshFI GGCC 1 cut(s) 404
BshNI GGYRCC 2 cut(s) 295, 530
BshVI ATCGAT 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 5 cut(s) 210, 236, 273, 347, 499
BslI CCNNNNNNNGG 2 cut(s) 159, 305
BsmAI GTCTC 4 cut(s) 127, 141, 344, 348
BsmBI CGTCTC 2 cut(s) 127, 348
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 404
BsoBI CYCGRG 1 cut(s) 498
Bsp13I TCCGGA 1 cut(s) 346
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 3 cut(s) 96, 178, 202
BspACI CCGC 2 cut(s) 86, 328
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 441
BspDI ATCGAT 1 cut(s) 514
BspEI TCCGGA 1 cut(s) 346
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 4 cut(s) 197, 297, 423, 532
BspQI GCTCTTC 1 cut(s) 600
BspT107I GGYRCC 2 cut(s) 295, 530
BsrGI TGTACA 1 cut(s) 606
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 96, 178, 202
BssNI GRCGYC 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 5 cut(s) 103, 226, 250, 355, 634
Bst6I CTCTTC 2 cut(s) 8, 600
BstACI GRCGYC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 1 cut(s) 449
BstF5I GGATG 4 cut(s) 205, 269, 508, 609
BstH2I RGCGCY 2 cut(s) 299, 594
BstHHI GCGC 2 cut(s) 298, 593
BstKTI GATC 3 cut(s) 99, 181, 205
BstMAI GTCTC 4 cut(s) 127, 141, 344, 348
BstMBI GATC 3 cut(s) 96, 178, 202
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 5 cut(s) 8, 208, 235, 497, 498
BstSNI TACGTA 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 330
Bsu15I ATCGAT 1 cut(s) 514
BsuRI GGCC 1 cut(s) 404
BsuTUI ATCGAT 1 cut(s) 514
BtsCI GGATG 4 cut(s) 205, 269, 508, 609
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 589
CaiI CAGNNNCTG 1 cut(s) 651
CciI TCATGA 1 cut(s) 181
CfoI GCGC 2 cut(s) 298, 593
Cfr13I GGNCC 2 cut(s) 403, 421
Cfr9I CCCGGG 1 cut(s) 498
ClaI ATCGAT 1 cut(s) 514
CseI GACGC 1 cut(s) 109
Csp6I GTAC 5 cut(s) 139, 246, 487, 568, 607
CviAII CATG 3 cut(s) 182, 200, 371
CviJI RGCY 7 cut(s) 64, 89, 198, 394, 404, 448, 497
CviKI_1 RGCY 7 cut(s) 64, 89, 198, 394, 404, 448, 497
CviQI GTAC 5 cut(s) 139, 246, 487, 568, 607
DdeI CTNAG 1 cut(s) 449
DinI GGCGCC 1 cut(s) 297
DpnI GATC 3 cut(s) 98, 180, 204
DpnII GATC 3 cut(s) 96, 178, 202
Eam1104I CTCTTC 2 cut(s) 8, 600
EarI CTCTTC 2 cut(s) 8, 600
Eco105I TACGTA 1 cut(s) 40
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 421
Eco47III AGCGCT 1 cut(s) 592
Eco88I CYCGRG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 8
EcoRV GATATC 1 cut(s) 187
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
Esp3I CGTCTC 2 cut(s) 127, 348
FaeI CATG 3 cut(s) 185, 203, 374
FaiI YATR 9 cut(s) 56, 183, 201, 368, 372, 417, 419, 548, 550
FatI CATG 3 cut(s) 181, 199, 370
FauNDI CATATG 1 cut(s) 417
FbaI TGATCA 2 cut(s) 178, 202
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 4 cut(s) 192, 256, 515, 596
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 2 cut(s) 30, 560
GlaI GCGC 2 cut(s) 297, 592
GluI GCNGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 2 cut(s) 299, 594
HaeIII GGCC 1 cut(s) 404
HapII CCGG 5 cut(s) 210, 236, 273, 347, 499
HgaI GACGC 1 cut(s) 109
HhaI GCGC 2 cut(s) 298, 593
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 3 cut(s) 185, 203, 374
Hin6I GCGC 2 cut(s) 296, 591
HinP1I GCGC 2 cut(s) 296, 591
HinfI GANTC 3 cut(s) 309, 464, 648
HpaII CCGG 5 cut(s) 210, 236, 273, 347, 499
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 2 cut(s) 127, 653
Hpy188III TCNNGA 3 cut(s) 182, 193, 347
Hpy8I GTNNAC 1 cut(s) 607
HpyAV CCTTC 1 cut(s) 581
HpyCH4III ACNGT 5 cut(s) 103, 226, 250, 355, 634
HpyCH4IV ACGT 2 cut(s) 39, 541
HpyCH4V TGCA 3 cut(s) 48, 443, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 2 cut(s) 39, 541
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 3 cut(s) 185, 203, 374
HspAI GCGC 2 cut(s) 296, 591
KasI GGCGCC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 346
Ksp22I TGATCA 2 cut(s) 178, 202
Kzo9I GATC 3 cut(s) 96, 178, 202
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 2 cut(s) 127, 195
LpnPI CCDG 8 cut(s) 22, 144, 178, 223, 249, 286, 360, 512
LweI GCATC 2 cut(s) 493, 520
MaeI CTAG 2 cut(s) 30, 560
MaeII ACGT 2 cut(s) 39, 541
MaeIII GTNAC 4 cut(s) 103, 226, 377, 638
MalI GATC 3 cut(s) 98, 180, 204
MboI GATC 3 cut(s) 96, 178, 202
MboII GAAGA 4 cut(s) 25, 181, 330, 587
MluCI AATT 3 cut(s) 110, 163, 218
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 473
MnlI CCTC 6 cut(s) 136, 233, 435, 444, 448, 501
MroI TCCGGA 1 cut(s) 346
MseI TTAA 2 cut(s) 435, 492
MspI CCGG 5 cut(s) 210, 236, 273, 347, 499
MspR9I CCNGG 5 cut(s) 10, 210, 237, 499, 500
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 503
NarI GGCGCC 1 cut(s) 296
NciI CCSGG 4 cut(s) 210, 237, 499, 500
NdeI CATATG 1 cut(s) 417
NdeII GATC 3 cut(s) 96, 178, 202
NlaIII CATG 3 cut(s) 185, 203, 374
NlaIV GGNNCC 4 cut(s) 197, 297, 423, 532
NmuCI GTSAC 3 cut(s) 103, 226, 377
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 370
PciSI GCTCTTC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 472
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 40
PscI ACATGT 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 197, 297, 423, 532
PspPI GGNCC 2 cut(s) 403, 421
PstNI CAGNNNCTG 1 cut(s) 651
RsaI GTAC 5 cut(s) 140, 247, 488, 569, 608
RsaNI GTAC 5 cut(s) 139, 246, 487, 568, 607
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 435, 492
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 3 cut(s) 96, 178, 202
Sau96I GGNCC 2 cut(s) 403, 421
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 5 cut(s) 10, 210, 237, 499, 500
SetI ASST 6 cut(s) 42, 396, 450, 492, 544, 573
SfaNI GCATC 2 cut(s) 493, 520
SfoI GGCGCC 1 cut(s) 297
SinI GGWCC 1 cut(s) 421
SmaI CCCGGG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SnaBI TACGTA 1 cut(s) 40
Sse9I AATT 3 cut(s) 110, 163, 218
SsiI CCGC 2 cut(s) 86, 328
SspDI GGCGCC 1 cut(s) 295
SspMI CTAG 2 cut(s) 30, 560
StyD4I CCNGG 5 cut(s) 8, 208, 235, 497, 498
TaaI ACNGT 5 cut(s) 103, 226, 250, 355, 634
TaiI ACGT 2 cut(s) 42, 544
TaqI TCGA 3 cut(s) 95, 216, 514
TasI AATT 3 cut(s) 110, 163, 218
TatI WGTACW 2 cut(s) 245, 606
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 2 cut(s) 435, 492
Tru9I TTAA 2 cut(s) 435, 492
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 3 cut(s) 103, 226, 377
Tsp45I GTSAC 3 cut(s) 103, 226, 377
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 2 cut(s) 324, 602
TspMI CCCGGG 1 cut(s) 498
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 2 cut(s) 163, 218
XceI RCATGY 1 cut(s) 374
XmaI CCCGGG 1 cut(s) 498
XspI CTAG 2 cut(s) 30, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.