pycom16g16930

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004390
Physical Location & Seq
Forward (+)
12935648 .. 12938843
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16930.1

Sequence Viewer

Length: 636 bp
ATGGAGGGAAGCGCGAAGGGTTTGATGCTGATTGGTCTTCTTCTCATGAGTTTCTTCGGAAAGATCGCATCGCTATCGGTGACAGTGAACGATGTCGAATGCTTGTACGAGTATGTGCTCTACGAAGGCGACACCGTTTCGGGCAACTTCGTGGTCGTCGATCACGATATTTTCTGGGGCGCGGATCATCCTGGCCTCGACTTAATCGTGACATCTCCTGGTGGTAATACGGTGCACACTTCGAAGGGAACATCTGGGGATAAGTTTGAGTTTAAAGCCCCAAGAAGTGGGATGTACAAATTTTGTTTCCACAATCCTTACTCAACACCAGAGACTGTTTCCTTTAACATTCATGTTGGACATATTCCCAACGAGCACAATATTGCAAAAGATGAGCATCTAGACCCTATAAATGTTAAAATTGCTGAGCTTAGAGAGGCGTTGGAATCAGTTACATTAGAGCAGAAGTACTTGAAAGCACGTGATGTTCGACATCGTCATACAAACGAGAGCACAAGGAGACGGGTTGTGTTGTATACAGTGGCAGAGTATCTTGCGCTGGCTGCTGCGAGTGCTTTACAAGTCGTATACATTCGCAAACTTTTCAGCAAATCGGTTGCATACAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

212

Amino Acids

23.59

Weight (kDa)

6.55

Isoelectric Point (pI)

28.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 27 - 202 5.9e-47 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 287
AccI GTMKAC 2 cut(s) 536, 588
AccII CGCG 2 cut(s) 14, 182
AciI CCGC 1 cut(s) 182
AclWI GGATC 1 cut(s) 192
AcsI RAATTY 1 cut(s) 299
AcvI CACGTG 1 cut(s) 482
AfaI GTAC 3 cut(s) 107, 296, 470
AfiI CCNNNNNNNGG 1 cut(s) 287
AgsI TTSAA 1 cut(s) 475
AjnI CCWGG 2 cut(s) 190, 217
AluBI AGCT 1 cut(s) 430
AluI AGCT 1 cut(s) 430
Alw21I GWGCWC 4 cut(s) 120, 237, 378, 515
Alw26I GTCTC 2 cut(s) 326, 514
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 193
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 563, 566
ApoI RAATTY 1 cut(s) 299
AspLEI GCGC 3 cut(s) 14, 182, 559
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 237
BbrPI CACGTG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 4 cut(s) 120, 237, 378, 515
BbvI GCAGC 2 cut(s) 550, 553
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 2 cut(s) 192, 219
BcoDI GTCTC 2 cut(s) 326, 514
BfaI CTAG 1 cut(s) 401
BisI GCNGC 2 cut(s) 564, 567
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 2 cut(s) 565, 568
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 2 cut(s) 192, 219
BmrFI CCNGG 2 cut(s) 192, 219
BmsI GCATC 3 cut(s) 15, 77, 406
BpiI GAAGAC 1 cut(s) 29
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 242
BsaAI YACGTR 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 396
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 192, 219
BseGI GGATG 2 cut(s) 187, 297
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 550, 553
Bsh1236I CGCG 2 cut(s) 14, 182
BshFI GGCC 1 cut(s) 195
BsiHKAI GWGCWC 4 cut(s) 120, 237, 378, 515
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 2 cut(s) 326, 514
BsmBI CGTCTC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 1 cut(s) 195
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 120, 237, 378, 515
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 3 cut(s) 63, 160, 184
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 1 cut(s) 182
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 2 cut(s) 14, 182
BspHI TCATGA 1 cut(s) 45
BspPI GGATC 1 cut(s) 192
BspT104I TTCGAA 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 294
BssMI GATC 3 cut(s) 63, 160, 184
BssNAI GTATAC 2 cut(s) 537, 589
Bst1107I GTATAC 2 cut(s) 537, 589
Bst2UI CCWGG 2 cut(s) 192, 219
Bst4CI ACNGT 5 cut(s) 85, 136, 232, 337, 541
BstAUI TGTACA 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 2 cut(s) 426, 431
BstF5I GGATG 2 cut(s) 187, 297
BstFNI CGCG 2 cut(s) 14, 182
BstHHI GCGC 3 cut(s) 14, 182, 559
BstKTI GATC 3 cut(s) 66, 163, 187
BstMAI GTCTC 2 cut(s) 326, 514
BstMBI GATC 3 cut(s) 63, 160, 184
BstMWI GCNNNNNNNGC 2 cut(s) 563, 572
BstNI CCWGG 2 cut(s) 192, 219
BstSCI CCNGG 2 cut(s) 190, 217
BstSLI GKGCMC 1 cut(s) 237
BstUI CGCG 2 cut(s) 14, 182
BstV1I GCAGC 2 cut(s) 550, 553
BstV2I GAAGAC 1 cut(s) 29
BstZ17I GTATAC 2 cut(s) 537, 589
BsuRI GGCC 1 cut(s) 195
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 2 cut(s) 187, 297
BtsIMutI CAGTG 2 cut(s) 90, 546
Cac8I GCNNGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 335
CciI TCATGA 1 cut(s) 45
CfoI GCGC 3 cut(s) 14, 182, 559
Csp6I GTAC 3 cut(s) 106, 295, 469
CviAII CATG 2 cut(s) 46, 353
CviJI RGCY 4 cut(s) 195, 278, 430, 563
CviKI_1 RGCY 4 cut(s) 195, 278, 430, 563
CviQI GTAC 3 cut(s) 106, 295, 469
DdeI CTNAG 2 cut(s) 426, 431
DpnI GATC 3 cut(s) 65, 162, 186
DpnII GATC 3 cut(s) 63, 160, 184
DraI TTTAAA 1 cut(s) 274
Eco72I CACGTG 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 190, 217
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 2 cut(s) 49, 356
FaiI YATR 9 cut(s) 47, 114, 354, 363, 410, 501, 537, 589, 622
FatI CATG 2 cut(s) 45, 352
FblI GTMKAC 2 cut(s) 536, 588
Fnu4HI GCNGC 2 cut(s) 564, 567
FokI GGATG 2 cut(s) 174, 304
Fsp4HI GCNGC 2 cut(s) 564, 567
FspBI CTAG 1 cut(s) 401
GlaI GCGC 3 cut(s) 13, 181, 558
GluI GCNGC 2 cut(s) 564, 567
HaeIII GGCC 1 cut(s) 195
HhaI GCGC 3 cut(s) 14, 182, 559
Hin1II CATG 2 cut(s) 49, 356
Hin6I GCGC 3 cut(s) 12, 180, 557
HinP1I GCGC 3 cut(s) 12, 180, 557
HinfI GANTC 1 cut(s) 446
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 88, 235, 537, 589
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 4 cut(s) 46, 164, 208, 401
Hpy8I GTNNAC 4 cut(s) 88, 235, 537, 589
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 10, 119, 238
HpyCH4III ACNGT 5 cut(s) 85, 136, 232, 337, 541
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 3 cut(s) 235, 386, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 572
HpyF3I CTNAG 2 cut(s) 426, 431
HpySE526I ACGT 1 cut(s) 481
Hsp92II CATG 2 cut(s) 49, 356
HspAI GCGC 3 cut(s) 12, 180, 557
Kzo9I GATC 3 cut(s) 63, 160, 184
LpnPI CCDG 8 cut(s) 160, 177, 204, 204, 231, 240, 342, 545
Lsp1109I GCAGC 2 cut(s) 550, 553
LweI GCATC 3 cut(s) 15, 77, 406
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 3 cut(s) 79, 208, 451
MalI GATC 3 cut(s) 65, 162, 186
MboI GATC 3 cut(s) 63, 160, 184
MboII GAAGA 3 cut(s) 29, 32, 46
MhlI GDGCHC 4 cut(s) 120, 237, 378, 515
MluCI AATT 2 cut(s) 299, 420
MmeI TCCRAC 2 cut(s) 337, 423
MnlI CCTC 2 cut(s) 206, 430
MseI TTAA 4 cut(s) 203, 273, 345, 417
MspR9I CCNGG 2 cut(s) 192, 219
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 2 cut(s) 192, 219
MvnI CGCG 2 cut(s) 14, 182
MwoI GCNNNNNNNGC 2 cut(s) 563, 572
NdeII GATC 3 cut(s) 63, 160, 184
NlaIII CATG 2 cut(s) 49, 356
NmuCI GTSAC 2 cut(s) 79, 208
NspV TTCGAA 1 cut(s) 242
PagI TCATGA 1 cut(s) 45
PcsI WCGNNNNNNNCGW 4 cut(s) 156, 162, 204, 487
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 446
PflFI GACNNNGTC 1 cut(s) 495
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 2 cut(s) 565, 568
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
Ppu21I YACGTR 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 190, 217
PspCI CACGTG 1 cut(s) 482
PspGI CCWGG 2 cut(s) 190, 217
PstNI CAGNNNCTG 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 495
RsaI GTAC 3 cut(s) 107, 296, 470
RsaNI GTAC 3 cut(s) 106, 295, 469
SaqAI TTAA 4 cut(s) 203, 273, 345, 417
SatI GCNGC 2 cut(s) 564, 567
Sau3AI GATC 3 cut(s) 63, 160, 184
ScaI AGTACT 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 192, 219
SduI GDGCHC 4 cut(s) 120, 237, 378, 515
SetI ASST 2 cut(s) 432, 484
SfaNI GCATC 3 cut(s) 15, 77, 406
SfuI TTCGAA 1 cut(s) 242
Sse9I AATT 2 cut(s) 299, 420
SsiI CCGC 1 cut(s) 182
SspI AATATT 1 cut(s) 382
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 190, 217
TaaI ACNGT 5 cut(s) 85, 136, 232, 337, 541
TaiI ACGT 1 cut(s) 484
TaqI TCGA 5 cut(s) 96, 159, 198, 242, 490
TasI AATT 2 cut(s) 299, 420
TatI WGTACW 2 cut(s) 294, 468
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 4 cut(s) 203, 273, 345, 417
Tru9I TTAA 4 cut(s) 203, 273, 345, 417
TscAI CASTG 2 cut(s) 90, 546
TseFI GTSAC 2 cut(s) 79, 208
TseI GCWGC 2 cut(s) 563, 566
Tsp45I GTSAC 2 cut(s) 79, 208
TspDTI ATGAA 1 cut(s) 341
TspRI CASTG 2 cut(s) 90, 546
Tth111I GACNNNGTC 1 cut(s) 495
Van91I CCANNNNNTGG 1 cut(s) 287
VneI GTGCAC 1 cut(s) 233
XapI RAATTY 1 cut(s) 299
XbaI TCTAGA 1 cut(s) 400
XmiI GTMKAC 2 cut(s) 536, 588
XspI CTAG 1 cut(s) 401
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.