MD11G1278600.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004390
Physical Location & Seq
Reverse (-)
39580638 .. 39582494
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1278600.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGAGAAAAGAAAGAGATTCGAGACCGCCAAGATTTACGTGGCGTACGGTTTTCTTCTGAGCCTCATCGGCAATGGCAGTGTTGCTACTGCTCTTTCAATCACCGTGACGGAGTTTGAATGCGTATCAGAGCAGGTTCTGCACGAGGGAGACACCGTCTCCGGAAACTTTGTTGCCATAGACCATGACATCTTCTGGAGCTCCGACCACCCTGGCATCGATTTCACTGCTACAGCTCCAGGAGGTAGCATTGTGTCTTCTCTGAAAGGAACGTCGGGAGATAAGTTCAAGTTCAAGGCACCAAAAAGCGGAATCTACAAGTTCTGTTTTCGAAATCCTGCTTCGACTCCTGAGACTGTTTCGTTCTACATCCATGTTGGCCACATCCCAAATGAGCATGAATTGGCTAAAGACGAACATATGGACCCTCTGAATATTAAAATTGCCGAGCTGAGAGAAGCATTGGAGGCTATCACAACGGAGCAAAAGTTCCTTAAAGCGCGCGATGCCAGGCATAGATATACAAATGTTAGCACGAACAAACGAGTGATCTACTTCACACTTGCAGAGTACCTTGTATTTGCCGGTGCAAGCGCTCTTCAAGTGCTCTACATCCGCAATCTGTTCAGCAAGTCCGTTGCTTATAACAGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

219

Amino Acids

24.34

Weight (kDa)

7.8

Isoelectric Point (pI)

23.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 37 - 209 5.1e-44 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 124
AccB1I GGYRCC 1 cut(s) 298
AccII CGCG 2 cut(s) 502, 504
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 3 cut(s) 27, 309, 616
AcoI YGGCCR 1 cut(s) 379
AfaI GTAC 2 cut(s) 47, 572
AfeI AGCGCT 1 cut(s) 595
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 5 cut(s) 99, 119, 289, 295, 602
AjnI CCWGG 3 cut(s) 211, 238, 509
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 3 cut(s) 201, 236, 451
AluI AGCT 3 cut(s) 201, 236, 451
Alw21I GWGCWC 2 cut(s) 203, 609
Alw26I GTCTC 4 cut(s) 17, 144, 163, 347
AlwNI CAGNNNCTG 1 cut(s) 139
Aor13HI TCCGGA 1 cut(s) 161
Aor51HI AGCGCT 1 cut(s) 595
AoxI GGCC 1 cut(s) 379
AspLEI GCGC 3 cut(s) 502, 504, 596
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 381
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 203
BauI CACGAG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 203, 609
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 3 cut(s) 213, 240, 511
BcoDI GTCTC 4 cut(s) 17, 144, 163, 347
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 69
Bme1390I CCNGG 3 cut(s) 213, 240, 511
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 300, 426
BmrFI CCNGG 3 cut(s) 213, 240, 511
BmsI GCATC 2 cut(s) 225, 496
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 2 cut(s) 217, 222
Bpu14I TTCGAA 1 cut(s) 331
Bsa29I ATCGAT 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 40
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 211
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 6 cut(s) 141, 143, 171, 173, 473, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 1 cut(s) 584
Bse3DI GCAATG 1 cut(s) 79
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 3 cut(s) 213, 240, 511
BseCI ATCGAT 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 3 cut(s) 369, 384, 612
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMI GCAATG 1 cut(s) 79
BseMII CTCAG 3 cut(s) 50, 342, 443
BsePI GCGCGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 125
Bsh1236I CGCG 2 cut(s) 502, 504
BshFI GGCC 1 cut(s) 381
BshNI GGYRCC 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 203, 609
BsiSI CCGG 2 cut(s) 162, 585
BsiWI CGTACG 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 4 cut(s) 17, 144, 163, 347
BsmBI CGTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 1 cut(s) 381
Bso31I GGTCTC 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 2 cut(s) 203, 609
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 1 cut(s) 549
BspACI CCGC 3 cut(s) 27, 309, 616
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 3 cut(s) 51, 343, 444
BspDI ATCGAT 1 cut(s) 219
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 2 cut(s) 502, 504
BspLI GGNNCC 2 cut(s) 300, 426
BspMI ACCTGC 1 cut(s) 124
BspQI GCTCTTC 1 cut(s) 603
BspT104I TTCGAA 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 584
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 211
BssHII GCGCGC 1 cut(s) 500
BssMI GATC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 3 cut(s) 213, 240, 511
Bst4CI ACNGT 4 cut(s) 50, 106, 157, 358
Bst6I CTCTTC 1 cut(s) 603
BstAPI GCANNNNNTGC 1 cut(s) 139
BstBAI YACGTR 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 502, 592
BstDEI CTNAG 3 cut(s) 59, 351, 452
BstF5I GGATG 3 cut(s) 369, 384, 612
BstFNI CGCG 2 cut(s) 502, 504
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 3 cut(s) 502, 504, 596
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 4 cut(s) 17, 144, 163, 347
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 4 cut(s) 69, 139, 467, 506
BstNI CCWGG 3 cut(s) 213, 240, 511
BstSCI CCNGG 3 cut(s) 211, 238, 509
BstSFI CTRYAG 1 cut(s) 231
BstUI CGCG 2 cut(s) 502, 504
BstV2I GAAGAC 1 cut(s) 249
Bsu15I ATCGAT 1 cut(s) 219
BsuRI GGCC 1 cut(s) 381
BsuTUI ATCGAT 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 519
BtsCI GGATG 3 cut(s) 369, 384, 612
BtsI GCAGTG 2 cut(s) 85, 225
BtsIMutI CAGTG 2 cut(s) 85, 225
BveI ACCTGC 1 cut(s) 124
Cac8I GCNNGC 2 cut(s) 502, 592
CaiI CAGNNNCTG 1 cut(s) 139
CfoI GCGC 3 cut(s) 502, 504, 596
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 219
Csp6I GTAC 2 cut(s) 46, 571
CviAII CATG 3 cut(s) 185, 374, 398
CviJI RGCY 7 cut(s) 63, 201, 236, 381, 407, 451, 470
CviKI_1 RGCY 7 cut(s) 63, 201, 236, 381, 407, 451, 470
CviQI GTAC 2 cut(s) 46, 571
DdeI CTNAG 3 cut(s) 59, 351, 452
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 424
Eco47III AGCGCT 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 201
EcoRII CCWGG 3 cut(s) 211, 238, 509
EcoT38I GRGCYC 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 3 cut(s) 188, 377, 401
FaiI YATR 9 cut(s) 179, 186, 375, 399, 420, 422, 516, 522, 645
FatI CATG 3 cut(s) 184, 373, 397
FauNDI CATATG 1 cut(s) 420
FokI GGATG 3 cut(s) 356, 371, 599
FriOI GRGCYC 1 cut(s) 203
GlaI GCGC 3 cut(s) 501, 503, 595
GsuI CTGGAG 2 cut(s) 217, 222
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 1 cut(s) 381
HapII CCGG 2 cut(s) 162, 585
HhaI GCGC 3 cut(s) 502, 504, 596
Hin1II CATG 3 cut(s) 188, 377, 401
Hin6I GCGC 3 cut(s) 500, 502, 594
HinP1I GCGC 3 cut(s) 500, 502, 594
HinfI GANTC 3 cut(s) 18, 312, 346
HpaII CCGG 2 cut(s) 162, 585
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 5 cut(s) 60, 130, 205, 264, 432
Hpy188III TCNNGA 5 cut(s) 22, 162, 196, 276, 350
Hpy99I CGWCG 1 cut(s) 277
HpyCH4III ACNGT 4 cut(s) 50, 106, 157, 358
HpyCH4IV ACGT 2 cut(s) 39, 272
HpyCH4V TGCA 3 cut(s) 142, 566, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 139, 467, 506
HpyF3I CTNAG 3 cut(s) 59, 351, 452
HpySE526I ACGT 2 cut(s) 39, 272
Hsp92II CATG 3 cut(s) 188, 377, 401
HspAI GCGC 3 cut(s) 500, 502, 594
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 1 cut(s) 549
LguI GCTCTTC 1 cut(s) 603
LmnI GCTCC 4 cut(s) 198, 206, 241, 481
LweI GCATC 2 cut(s) 225, 496
MaeII ACGT 2 cut(s) 39, 272
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 4 cut(s) 47, 184, 249, 590
MhlI GDGCHC 2 cut(s) 203, 609
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 2 cut(s) 401, 441
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 5 cut(s) 74, 139, 236, 438, 460
Mox20I TGGCCA 1 cut(s) 381
MroI TCCGGA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 381
MseI TTAA 3 cut(s) 438, 495, 655
Msp20I TGGCCA 1 cut(s) 381
MspI CCGG 2 cut(s) 162, 585
MspR9I CCNGG 3 cut(s) 213, 240, 511
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 3 cut(s) 213, 240, 511
MvnI CGCG 2 cut(s) 502, 504
MwoI GCNNNNNNNGC 4 cut(s) 69, 139, 467, 506
NdeI CATATG 1 cut(s) 420
NdeII GATC 1 cut(s) 549
NlaIII CATG 3 cut(s) 188, 377, 401
NlaIV GGNNCC 2 cut(s) 300, 426
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 106
NspV TTCGAA 1 cut(s) 331
PauI GCGCGC 1 cut(s) 500
PciSI GCTCTTC 1 cut(s) 603
PctI GAATGC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 18, 312
Pfl23II CGTACG 1 cut(s) 45
PflFI GACNNNGTC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 238
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Ppu21I YACGTR 1 cut(s) 40
PsiI TTATAA 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 211, 238, 509
PspGI CCWGG 3 cut(s) 211, 238, 509
PspLI CGTACG 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 300, 426
PspPI GGNCC 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 139
PsyI GACNNNGTC 1 cut(s) 155
PteI GCGCGC 1 cut(s) 500
RsaI GTAC 2 cut(s) 47, 572
RsaNI GTAC 2 cut(s) 46, 571
SacI GAGCTC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 3 cut(s) 438, 495, 655
Sau3AI GATC 1 cut(s) 549
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 3 cut(s) 213, 240, 511
SduI GDGCHC 2 cut(s) 203, 609
SetI ASST 8 cut(s) 42, 138, 203, 238, 247, 275, 453, 576
SfaNI GCATC 2 cut(s) 225, 496
SfcI CTRYAG 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 331
SinI GGWCC 1 cut(s) 424
Sse9I AATT 2 cut(s) 401, 441
SsiI CCGC 3 cut(s) 27, 309, 616
SspI AATATT 1 cut(s) 436
SstI GAGCTC 1 cut(s) 203
StyD4I CCNGG 3 cut(s) 211, 238, 509
TaaI ACNGT 4 cut(s) 50, 106, 157, 358
TaiI ACGT 2 cut(s) 42, 275
TaqI TCGA 4 cut(s) 21, 219, 331, 344
TasI AATT 2 cut(s) 401, 441
TfiI GAWTC 2 cut(s) 18, 312
Tru1I TTAA 3 cut(s) 438, 495, 655
Tru9I TTAA 3 cut(s) 438, 495, 655
TscAI CASTG 2 cut(s) 85, 232
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 414
TspGWI ACGGA 3 cut(s) 125, 494, 625
TspRI CASTG 2 cut(s) 85, 232
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.