Rh4BG014800

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004390
Physical Location & Seq
Forward (+)
2125254 .. 2127872
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG014800.1

Sequence Viewer

Length: 645 bp
ATGGAGTCTTGGCAGAGCAGAAAAGGCGTGATCTTGATGGGGATTCTATTATTGATGAGCTTTGTGGGGCGAATCGCGTCGCTATCGGTGACGGTGAACGACATGGAATGCGTGTACGAGTATGTGCTCTACGAAGACGACGTCGTTTCGGGGAACTTCGTGGTGGTCGACCATGATATCTTCTGGGCCAGCGATCATCCGGGTATCGATTTAATTGTGACATCTCCTGGGGGTAATACAGTGCACACTTCAAAGGGAACATCTGGAGATAAGTTTGAGTTTAAGGCTCCAAGGAGTGGAATGTACAAGTTCTGTTTTCACAATCCCTACTCAACTCCAGAAACGGTTTCATTCAATATACATGTCGGCCATATTCCTACTGAGCACAACATTGCCAAGGATGAACATCTAGACCCTATTAATGTGAAGATAGCTGAGCTTAGAGAGGCATTGGAGTCAGTTACATTGGAACAAAAGTACTTGAAAGCACGTGATGCTCGCCATCGTCATACAAATGAGAGCACAAGAAAGCGTGTTGTGCTCTACACAGTGGGAGAGTACTTCATGCTAGTTGCTGCAAGTGCTTTACAAGTTGTATACATCCGCCGCCTCTTTAGCAAATCAATTGGATATAATCGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

214

Amino Acids

24.27

Weight (kDa)

6.55

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 30 - 206 3e-48 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 2 cut(s) 168, 597
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 604, 607
AcoI YGGCCR 1 cut(s) 367
AcvI CACGTG 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 4 cut(s) 116, 305, 479, 560
AfiI CCNNNNNNNGG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 3 cut(s) 252, 355, 484
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 3 cut(s) 60, 434, 439
AluI AGCT 3 cut(s) 60, 434, 439
Alw21I GWGCWC 5 cut(s) 129, 246, 387, 524, 543
Alw44I GTGCAC 1 cut(s) 242
AoxI GGCC 2 cut(s) 186, 367
ApaLI GTGCAC 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 575
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AseI ATTAAT 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 100, 106
BaeGI GKGCMC 1 cut(s) 246
BbrPI CACGTG 1 cut(s) 491
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 5 cut(s) 129, 246, 387, 524, 543
BbvI GCAGC 1 cut(s) 562
BccI CCATC 2 cut(s) 31, 510
BciT130I CCWGG 1 cut(s) 228
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 2 cut(s) 410, 569
BisI GCNGC 2 cut(s) 576, 607
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 2 cut(s) 577, 608
BmcAI AGTACT 2 cut(s) 479, 560
Bme1390I CCNGG 2 cut(s) 201, 228
BmgT120I GGNCC 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 2 cut(s) 201, 228
BmsI GCATC 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 285, 321
Bpu1102I GCTNAGC 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 227, 290, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 390
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 3 cut(s) 227, 290, 396
BseGI GGATG 3 cut(s) 196, 406, 600
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 2 cut(s) 372, 426
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 1 cut(s) 562
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 188, 369
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 5 cut(s) 129, 246, 387, 524, 543
BsiSI CCGG 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 296
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 2 cut(s) 188, 369
Bsp1286I GDGCHC 5 cut(s) 129, 246, 387, 524, 543
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 2 cut(s) 30, 193
Bsp1720I GCTNAGC 1 cut(s) 435
BspACI CCGC 2 cut(s) 604, 607
BspANI GGCC 2 cut(s) 188, 369
BspCNI CTCAG 2 cut(s) 373, 427
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 3 cut(s) 227, 290, 396
BssMI GATC 2 cut(s) 30, 193
BssNAI GTATAC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 290, 396
Bst1107I GTATAC 1 cut(s) 598
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 4 cut(s) 94, 241, 346, 550
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 190, 499
BstDEI CTNAG 3 cut(s) 381, 435, 440
BstF5I GGATG 3 cut(s) 196, 406, 600
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 2 cut(s) 33, 196
BstMBI GATC 2 cut(s) 30, 193
BstMWI GCNNNNNNNGC 5 cut(s) 24, 494, 538, 581, 615
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 199, 226
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 1 cut(s) 141
BstZ17I GTATAC 1 cut(s) 598
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 188, 369
BsuTUI ATCGAT 1 cut(s) 207
BtsCI GGATG 3 cut(s) 196, 406, 600
BtsIMutI CAGTG 2 cut(s) 246, 555
Cac8I GCNNGC 2 cut(s) 190, 499
Cfr13I GGNCC 1 cut(s) 186
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 66
Csp6I GTAC 4 cut(s) 115, 304, 478, 559
CviAII CATG 4 cut(s) 103, 173, 362, 565
CviJI RGCY 6 cut(s) 60, 188, 287, 369, 434, 439
CviKI_1 RGCY 6 cut(s) 60, 188, 287, 369, 434, 439
CviQI GTAC 4 cut(s) 115, 304, 478, 559
DdeI CTNAG 3 cut(s) 381, 435, 440
DpnI GATC 2 cut(s) 32, 195
DpnII GATC 2 cut(s) 30, 193
EaeI YGGCCR 1 cut(s) 367
EciI GGCGGA 1 cut(s) 593
Eco130I CCWWGG 2 cut(s) 290, 396
Eco32I GATATC 1 cut(s) 178
Eco72I CACGTG 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 226
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 2 cut(s) 290, 396
ErhI CCWWGG 2 cut(s) 290, 396
FaeI CATG 4 cut(s) 106, 176, 365, 568
FatI CATG 4 cut(s) 102, 172, 361, 564
FblI GTMKAC 2 cut(s) 168, 597
Fnu4HI GCNGC 2 cut(s) 576, 607
FokI GGATG 3 cut(s) 183, 413, 587
Fsp4HI GCNGC 2 cut(s) 576, 607
FspBI CTAG 2 cut(s) 410, 569
GluI GCNGC 2 cut(s) 576, 607
GsuI CTGGAG 2 cut(s) 285, 321
HaeIII GGCC 2 cut(s) 188, 369
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 66
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 4 cut(s) 106, 176, 365, 568
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 4 cut(s) 5, 43, 72, 455
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 100, 106
Hpy166II GTNNAC 5 cut(s) 97, 115, 169, 244, 598
Hpy188III TCNNGA 4 cut(s) 34, 264, 338, 410
Hpy8I GTNNAC 5 cut(s) 97, 115, 169, 244, 598
Hpy99I CGWCG 3 cut(s) 82, 143, 146
HpyCH4III ACNGT 4 cut(s) 94, 241, 346, 550
HpyCH4IV ACGT 2 cut(s) 141, 490
HpyCH4V TGCA 2 cut(s) 244, 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 494, 538, 581, 615
HpyF3I CTNAG 3 cut(s) 381, 435, 440
HpySE526I ACGT 2 cut(s) 141, 490
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 4 cut(s) 106, 176, 365, 568
Kzo9I GATC 2 cut(s) 30, 193
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 7 cut(s) 169, 202, 213, 213, 240, 249, 351
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 1 cut(s) 484
MaeI CTAG 2 cut(s) 410, 569
MaeII ACGT 2 cut(s) 141, 490
MaeIII GTNAC 3 cut(s) 88, 217, 460
MalI GATC 2 cut(s) 32, 195
MboI GATC 2 cut(s) 30, 193
MboII GAAGA 3 cut(s) 146, 172, 439
MfeI CAATTG 1 cut(s) 624
MhlI GDGCHC 5 cut(s) 129, 246, 387, 524, 543
MluCI AATT 2 cut(s) 213, 624
MlyI GAGTC 2 cut(s) 14, 464
MnlI CCTC 2 cut(s) 439, 620
MseI TTAA 3 cut(s) 212, 282, 420
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 201, 228
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 5 cut(s) 24, 494, 538, 581, 615
NciI CCSGG 1 cut(s) 201
NdeII GATC 2 cut(s) 30, 193
NlaIII CATG 4 cut(s) 106, 176, 365, 568
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 2 cut(s) 88, 217
NspI RCATGY 1 cut(s) 365
PciI ACATGT 1 cut(s) 361
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 165
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 43, 72
PflFI GACNNNGTC 1 cut(s) 140
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 2 cut(s) 577, 608
PleI GAGTC 2 cut(s) 13, 463
PmaCI CACGTG 1 cut(s) 491
PmlI CACGTG 1 cut(s) 491
PpsI GAGTC 2 cut(s) 13, 463
Ppu21I YACGTR 1 cut(s) 491
PscI ACATGT 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 226
PspCI CACGTG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 186
PsyI GACNNNGTC 1 cut(s) 140
RsaI GTAC 4 cut(s) 116, 305, 479, 560
RsaNI GTAC 4 cut(s) 115, 304, 478, 559
RseI CAYNNNNRTG 1 cut(s) 513
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 3 cut(s) 212, 282, 420
SatI GCNGC 2 cut(s) 576, 607
Sau3AI GATC 2 cut(s) 30, 193
Sau96I GGNCC 1 cut(s) 186
ScaI AGTACT 2 cut(s) 479, 560
SchI GAGTC 2 cut(s) 14, 464
ScrFI CCNGG 2 cut(s) 201, 228
SduI GDGCHC 5 cut(s) 129, 246, 387, 524, 543
SetI ASST 5 cut(s) 62, 144, 436, 441, 493
SfaNI GCATC 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 513
Sse9I AATT 2 cut(s) 213, 624
SsiI CCGC 2 cut(s) 604, 607
SspMI CTAG 2 cut(s) 410, 569
StyD4I CCNGG 2 cut(s) 199, 226
StyI CCWWGG 2 cut(s) 290, 396
TaaI ACNGT 4 cut(s) 94, 241, 346, 550
TaiI ACGT 2 cut(s) 144, 493
TaqI TCGA 2 cut(s) 168, 207
TasI AATT 2 cut(s) 213, 624
TatI WGTACW 3 cut(s) 303, 477, 558
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 2 cut(s) 43, 72
Tru1I TTAA 3 cut(s) 212, 282, 420
Tru9I TTAA 3 cut(s) 212, 282, 420
TscAI CASTG 2 cut(s) 246, 555
TseFI GTSAC 2 cut(s) 88, 217
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 2 cut(s) 88, 217
TspDTI ATGAA 3 cut(s) 339, 417, 553
TspRI CASTG 2 cut(s) 246, 555
Tth111I GACNNNGTC 1 cut(s) 140
Van91I CCANNNNNTGG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 242
VspI ATTAAT 1 cut(s) 420
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 1 cut(s) 365
XmiI GTMKAC 2 cut(s) 168, 597
XspI CTAG 2 cut(s) 410, 569
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 2 cut(s) 479, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.