MD03G1257900.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004390
Physical Location & Seq
Reverse (-)
34513832 .. 34515048
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1257900.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGAGAAACGAAAGCGATTTGAGACCGCCAAGATTTGCGTGGCGTACGGTTTTCTTATGAGCTTCATCGGCAATGGCAATGTTGCTACTGCCCTTTCAATCACCGTGACGGAGTTGGAATGCGTCTCGGAACAAGTTCTACACGAGGGAGACACGGTCTCTGGAAACTTTGTTGTAATCGACCACGACATCTTCTGGAGCTCCGACCACCCTGGTATTGATTTCACTGCCACAGCTCCAGGAGGCGGTATTGTGTTTTCGCTGAAAGGAACGTCTGGGGATAAGTTCGCGTTCAAGGCACCGAAAAGCGGAATGTACAAGTTTTGTTTTCGCAATCCTGTTTCGACTCATGAGACTGTTTCGTTCTACATTCATGTTGGCCACATCCCAAATGACCATGACTTAGCCAAAGACGAACATATGGACCCTTTGAATATTAAGATTGCTGAGCTAAGAGAAGCACTGGAAGTTGTCACAACAGAGCAAAAGTACCTTAAAGCACGCGATGCTAGGCATCGATATACAAATGTTAGCACGAATAAACGAGTGATCTACTTCACACTTGCAGAGTACCTTGTGTTTGCTGGTGCAAGTGCTCTTCAAGTGTTGTACATCCGCAACCTGTTTAGCAAATCAGTTGCCTATAACAGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

219

Amino Acids

24.41

Weight (kDa)

7.12

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 36 - 209 3e-45 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 298
AccII CGCG 2 cut(s) 290, 504
AciI CCGC 4 cut(s) 27, 246, 309, 616
AcoI YGGCCR 1 cut(s) 379
AfaI GTAC 5 cut(s) 47, 317, 491, 572, 611
AfiI CCNNNNNNNGG 2 cut(s) 245, 308
AgsI TTSAA 4 cut(s) 99, 295, 433, 602
AjnI CCWGG 2 cut(s) 211, 238
AluBI AGCT 4 cut(s) 63, 201, 236, 451
AluI AGCT 4 cut(s) 63, 201, 236, 451
Alw21I GWGCWC 2 cut(s) 203, 598
Alw26I GTCTC 5 cut(s) 17, 130, 144, 163, 347
AoxI GGCC 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 381
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 203
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 2 cut(s) 203, 598
BciT130I CCWGG 2 cut(s) 213, 240
BcoDI GTCTC 5 cut(s) 17, 130, 144, 163, 347
BfaI CTAG 1 cut(s) 510
BlpI GCTNAGC 1 cut(s) 447
Bme1390I CCNGG 2 cut(s) 213, 240
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 300, 426
BmrFI CCNGG 2 cut(s) 213, 240
BmsI GCATC 2 cut(s) 496, 523
BpmI CTGGAG 2 cut(s) 217, 222
Bpu1102I GCTNAGC 1 cut(s) 447
Bsa29I ATCGAT 1 cut(s) 517
BsaI GGTCTC 2 cut(s) 17, 163
BsaJI CCNNGG 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 308
Bse1I ACTGG 1 cut(s) 468
Bse3DI GCAATG 2 cut(s) 79, 85
BseBI CCWGG 2 cut(s) 213, 240
BseCI ATCGAT 1 cut(s) 517
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 2 cut(s) 384, 612
BseLI CCNNNNNNNGG 2 cut(s) 245, 308
BseMI GCAATG 2 cut(s) 79, 85
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 290, 504
BshFI GGCC 1 cut(s) 381
BshNI GGYRCC 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 203, 598
BsiWI CGTACG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 245, 308
BsmAI GTCTC 5 cut(s) 17, 130, 144, 163, 347
BsmBI CGTCTC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 1 cut(s) 381
Bso31I GGTCTC 2 cut(s) 17, 163
Bsp1286I GDGCHC 2 cut(s) 203, 598
Bsp1407I TGTACA 2 cut(s) 315, 609
Bsp143I GATC 1 cut(s) 549
Bsp1720I GCTNAGC 1 cut(s) 447
BspACI CCGC 4 cut(s) 27, 246, 309, 616
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 1 cut(s) 439
BspDI ATCGAT 1 cut(s) 517
BspFNI CGCG 2 cut(s) 290, 504
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 300, 426
BspQI GCTCTTC 1 cut(s) 603
BspT107I GGYRCC 1 cut(s) 298
BspTNI GGTCTC 2 cut(s) 17, 163
BsrDI GCAATG 2 cut(s) 79, 85
BsrGI TGTACA 2 cut(s) 315, 609
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 2 cut(s) 213, 240
Bst4CI ACNGT 4 cut(s) 50, 106, 157, 358
Bst6I CTCTTC 1 cut(s) 603
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 2 cut(s) 315, 609
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 3 cut(s) 403, 447, 452
BstF5I GGATG 2 cut(s) 384, 612
BstFNI CGCG 2 cut(s) 290, 504
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 5 cut(s) 17, 130, 144, 163, 347
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 3 cut(s) 69, 296, 506
BstNI CCWGG 2 cut(s) 213, 240
BstSCI CCNGG 2 cut(s) 211, 238
BstUI CGCG 2 cut(s) 290, 504
Bsu15I ATCGAT 1 cut(s) 517
BsuRI GGCC 1 cut(s) 381
BsuTUI ATCGAT 1 cut(s) 517
BtgZI GCGATG 1 cut(s) 519
BtsCI GGATG 2 cut(s) 384, 612
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 461
Cac8I GCNNGC 1 cut(s) 502
CciI TCATGA 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 517
CseI GACGC 1 cut(s) 112
Csp6I GTAC 5 cut(s) 46, 316, 490, 571, 610
CviAII CATG 3 cut(s) 350, 374, 398
CviJI RGCY 6 cut(s) 63, 201, 236, 381, 407, 451
CviKI_1 RGCY 6 cut(s) 63, 201, 236, 381, 407, 451
CviQI GTAC 5 cut(s) 46, 316, 490, 571, 610
DdeI CTNAG 3 cut(s) 403, 447, 452
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 203
Eco31I GGTCTC 2 cut(s) 17, 163
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 211, 238
EcoT38I GRGCYC 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 3 cut(s) 353, 377, 401
FaiI YATR 8 cut(s) 59, 351, 375, 399, 420, 422, 522, 645
FatI CATG 3 cut(s) 349, 373, 397
FauNDI CATATG 1 cut(s) 420
FokI GGATG 2 cut(s) 371, 599
FriOI GRGCYC 1 cut(s) 203
FspBI CTAG 1 cut(s) 510
GsuI CTGGAG 2 cut(s) 217, 222
HaeIII GGCC 1 cut(s) 381
HgaI GACGC 1 cut(s) 112
Hin1II CATG 3 cut(s) 353, 377, 401
HinfI GANTC 1 cut(s) 346
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 130, 205
Hpy188III TCNNGA 3 cut(s) 162, 196, 350
HpyCH4III ACNGT 4 cut(s) 50, 106, 157, 358
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 566, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 296, 506
HpyF3I CTNAG 3 cut(s) 403, 447, 452
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 3 cut(s) 353, 377, 401
Kzo9I GATC 1 cut(s) 549
LguI GCTCTTC 1 cut(s) 603
LmnI GCTCC 3 cut(s) 198, 206, 241
LweI GCATC 2 cut(s) 496, 523
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 106, 472
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 2 cut(s) 184, 590
MhlI GDGCHC 2 cut(s) 203, 598
MlsI TGGCCA 1 cut(s) 381
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 2 cut(s) 96, 228
MnlI CCTC 2 cut(s) 139, 236
Mox20I TGGCCA 1 cut(s) 381
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 1 cut(s) 381
MseI TTAA 2 cut(s) 438, 495
Msp20I TGGCCA 1 cut(s) 381
MspR9I CCNGG 2 cut(s) 213, 240
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 2 cut(s) 213, 240
MvnI CGCG 2 cut(s) 290, 504
MwoI GCNNNNNNNGC 3 cut(s) 69, 296, 506
NdeI CATATG 1 cut(s) 420
NdeII GATC 1 cut(s) 549
NlaIII CATG 3 cut(s) 353, 377, 401
NlaIV GGNNCC 2 cut(s) 300, 426
NmuCI GTSAC 2 cut(s) 106, 472
PagI TCATGA 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 603
PctI GAATGC 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 135
Pfl23II CGTACG 1 cut(s) 45
PflFI GACNNNGTC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 238
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Psp124BI GAGCTC 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 211, 238
PspGI CCWGG 2 cut(s) 211, 238
PspLI CGTACG 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 300, 426
PspPI GGNCC 1 cut(s) 424
PsrI GAACNNNNNNTAC 2 cut(s) 123, 155
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 5 cut(s) 47, 317, 491, 572, 611
RsaNI GTAC 5 cut(s) 46, 316, 490, 571, 610
SacI GAGCTC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 2 cut(s) 438, 495
Sau3AI GATC 1 cut(s) 549
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 213, 240
SduI GDGCHC 2 cut(s) 203, 598
SetI ASST 8 cut(s) 65, 203, 238, 275, 453, 495, 576, 624
SfaNI GCATC 2 cut(s) 496, 523
SinI GGWCC 1 cut(s) 424
SsiI CCGC 4 cut(s) 27, 246, 309, 616
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 211, 238
TaaI ACNGT 4 cut(s) 50, 106, 157, 358
TaiI ACGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 180, 344, 517
TatI WGTACW 2 cut(s) 315, 609
Tru1I TTAA 2 cut(s) 438, 495
Tru9I TTAA 2 cut(s) 438, 495
TscAI CASTG 2 cut(s) 232, 468
TseFI GTSAC 2 cut(s) 106, 472
Tsp45I GTSAC 2 cut(s) 106, 472
TspDTI ATGAA 2 cut(s) 55, 362
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 232, 468
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.