Rh6CG332200

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004390
Physical Location & Seq
Reverse (-)
51960359 .. 51968331
7973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG332200.1

Sequence Viewer

Length: 744 bp
ATGGAGACGAGGAAGAGATTTGAGTGTGCCAAATTATACGTAGCATGCGGTTTTATGTTGTGCCTTATTGGCCATAGGAATGTTGCTGCTCTTTCGATCACTGTGACTGATGTGGAATGCGTTTCGGAGCTAGTTTTGTACGAGGGAGACACCATTTCTGGGAATTTTGTTGCCATTGATCATGATATCTTCTGGAGCCATGATCATCCCGGTCTCGAATTCACCGTAACATCTCCGGGAGGCAGTACAGTATTTTCTCTCAAAGGAACATCGGGCGACAAGTTTGAACTCAAGGCGCCACAAGGCGGAATCTACAAGTTCTGTTTCCGCAATCCGGCTCAAACTCCGGAGACTGTGTCTTTCTACATACATGTTGGTCACATTCCAACTGAGCATGACTTGGCCCAAGATGAACATATGGACCCTCTAAATATTAAGATTGCCGAGCTGAGGGAGGCATTGGAGTCTATCAGAGCAGAGCAAAAGTACCTTAAAGCCCGGGATGCAAGGCATCGATACACAAATCAAAGCACCAAAAAACGTGTTATCTACTTCACACTAGCAGAGTACATTGTCTTTGCCGGTGCAAGTGCTCTTCAAGTGGTGTACATCCGCAATCTGTTCAGCAAGACGAGAGAAAGGTCAATGAGGGCTGTTGCAAATGGACCTAATCGTATTTTAGCAATAAAACTATACATCTATTGTGGTATTAGCAGCACTTTTAAAAGACATGTTTATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

247

Amino Acids

28.0

Weight (kDa)

8.46

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 37 - 208 2.1e-41 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 4 cut(s) 48, 306, 328, 613
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 2 cut(s) 163, 218
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 5 cut(s) 140, 247, 488, 569, 608
AfiI CCNNNNNNNGG 4 cut(s) 159, 305, 334, 450
AflIII ACRYGT 3 cut(s) 370, 541, 730
AgsI TTSAA 2 cut(s) 287, 599
AluBI AGCT 2 cut(s) 130, 448
AluI AGCT 2 cut(s) 130, 448
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 3 cut(s) 141, 218, 344
Ama87I CYCGRG 1 cut(s) 498
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 2 cut(s) 70, 402
ApeKI GCWGC 2 cut(s) 86, 714
ApoI RAATTY 2 cut(s) 163, 218
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 3 cut(s) 403, 421, 665
AsuC2I CCSGG 4 cut(s) 210, 237, 499, 500
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 2 cut(s) 421, 665
BalI TGGCCA 1 cut(s) 72
BanI GGYRCC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 595
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 2 cut(s) 73, 726
BclI TGATCA 2 cut(s) 178, 202
BcnI CCSGG 4 cut(s) 210, 237, 499, 500
BcoDI GTCTC 3 cut(s) 141, 218, 344
BfaI CTAG 2 cut(s) 131, 560
BfoI RGCGCY 1 cut(s) 299
BglI GCCNNNNNGGC 1 cut(s) 69
BisI GCNGC 2 cut(s) 87, 715
BlsI GCNGC 2 cut(s) 88, 716
Bme1390I CCNGG 4 cut(s) 210, 237, 499, 500
Bme18I GGWCC 2 cut(s) 421, 665
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 3 cut(s) 403, 421, 665
BmiI GGNNCC 3 cut(s) 197, 297, 423
BmrFI CCNGG 4 cut(s) 210, 237, 499, 500
BmsI GCATC 2 cut(s) 493, 520
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 4 cut(s) 210, 237, 499, 500
Bsa29I ATCGAT 1 cut(s) 514
BsaAI YACGTR 1 cut(s) 40
BsaHI GRCGYC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 498
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 305, 334, 450
Bse118I RCCGGY 1 cut(s) 581
BseAI TCCGGA 1 cut(s) 346
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 3 cut(s) 205, 508, 609
BseLI CCNNNNNNNGG 4 cut(s) 159, 305, 334, 450
BseMII CTCAG 2 cut(s) 381, 440
BseXI GCAGC 2 cut(s) 73, 726
BshFI GGCC 2 cut(s) 72, 404
BshNI GGYRCC 1 cut(s) 295
BshVI ATCGAT 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 595
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 6 cut(s) 210, 236, 335, 347, 499, 582
BslI CCNNNNNNNGG 4 cut(s) 159, 305, 334, 450
BsmAI GTCTC 3 cut(s) 141, 218, 344
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 2 cut(s) 72, 404
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 595
Bsp13I TCCGGA 1 cut(s) 346
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 3 cut(s) 96, 178, 202
BspACI CCGC 4 cut(s) 48, 306, 328, 613
BspANI GGCC 2 cut(s) 72, 404
BspCNI CTCAG 2 cut(s) 382, 441
BspDI ATCGAT 1 cut(s) 514
BspEI TCCGGA 1 cut(s) 346
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 197, 297, 423
BspQI GCTCTTC 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 295
BspTNI GGTCTC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 581
BsrGI TGTACA 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 96, 178, 202
BssNI GRCGYC 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 103, 226, 250, 355
Bst6I CTCTTC 2 cut(s) 8, 600
BstACI GRCGYC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 390, 449
BstF5I GGATG 3 cut(s) 205, 508, 609
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 3 cut(s) 99, 181, 205
BstMAI GTCTC 3 cut(s) 141, 218, 344
BstMBI GATC 3 cut(s) 96, 178, 202
BstMWI GCNNNNNNNGC 2 cut(s) 69, 503
BstNSI RCATGY 3 cut(s) 48, 374, 734
BstSCI CCNGG 4 cut(s) 208, 235, 497, 498
BstSNI TACGTA 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 73, 726
Bsu15I ATCGAT 1 cut(s) 514
BsuRI GGCC 2 cut(s) 72, 404
BsuTUI ATCGAT 1 cut(s) 514
BtsCI GGATG 3 cut(s) 205, 508, 609
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 46
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 3 cut(s) 403, 421, 665
Cfr9I CCCGGG 1 cut(s) 498
ClaI ATCGAT 1 cut(s) 514
Csp6I GTAC 5 cut(s) 139, 246, 487, 568, 607
CviAII CATG 6 cut(s) 45, 182, 200, 371, 395, 731
CviJI RGCY 8 cut(s) 72, 130, 198, 338, 404, 448, 497, 653
CviKI_1 RGCY 8 cut(s) 72, 130, 198, 338, 404, 448, 497, 653
CviQI GTAC 5 cut(s) 139, 246, 487, 568, 607
DdeI CTNAG 2 cut(s) 390, 449
DinI GGCGCC 1 cut(s) 297
DpnI GATC 3 cut(s) 98, 180, 204
DpnII GATC 3 cut(s) 96, 178, 202
DraI TTTAAA 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 2 cut(s) 8, 600
EarI CTCTTC 2 cut(s) 8, 600
EciI GGCGGA 1 cut(s) 321
Eco105I TACGTA 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 2 cut(s) 421, 665
Eco88I CYCGRG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 218
EcoRV GATATC 1 cut(s) 187
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
FaeI CATG 6 cut(s) 48, 185, 203, 374, 398, 734
FatI CATG 6 cut(s) 44, 181, 199, 370, 394, 730
FauNDI CATATG 1 cut(s) 417
FbaI TGATCA 2 cut(s) 178, 202
Fnu4HI GCNGC 2 cut(s) 87, 715
FokI GGATG 3 cut(s) 192, 515, 596
Fsp4HI GCNGC 2 cut(s) 87, 715
FspBI CTAG 2 cut(s) 131, 560
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 87, 715
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 2 cut(s) 72, 404
HapII CCGG 6 cut(s) 210, 236, 335, 347, 499, 582
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 6 cut(s) 48, 185, 203, 374, 398, 734
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HinfI GANTC 2 cut(s) 309, 464
HpaII CCGG 6 cut(s) 210, 236, 335, 347, 499, 582
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 2 cut(s) 127, 473
Hpy188III TCNNGA 4 cut(s) 182, 193, 215, 347
Hpy8I GTNNAC 1 cut(s) 607
HpyCH4III ACNGT 4 cut(s) 103, 226, 250, 355
HpyCH4IV ACGT 2 cut(s) 39, 541
HpyCH4V TGCA 3 cut(s) 506, 587, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 503
HpyF3I CTNAG 2 cut(s) 390, 449
HpySE526I ACGT 2 cut(s) 39, 541
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 6 cut(s) 48, 185, 203, 374, 398, 734
HspAI GCGC 1 cut(s) 296
KasI GGCGCC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 346
Ksp22I TGATCA 2 cut(s) 178, 202
Kzo9I GATC 3 cut(s) 96, 178, 202
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 2 cut(s) 127, 195
LpnPI CCDG 8 cut(s) 144, 178, 223, 249, 348, 360, 512, 595
Lsp1109I GCAGC 2 cut(s) 73, 726
LweI GCATC 2 cut(s) 493, 520
MaeI CTAG 2 cut(s) 131, 560
MaeII ACGT 2 cut(s) 39, 541
MaeIII GTNAC 3 cut(s) 103, 226, 377
MalI GATC 3 cut(s) 98, 180, 204
MboI GATC 3 cut(s) 96, 178, 202
MboII GAAGA 3 cut(s) 25, 181, 587
MhlI GDGCHC 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 3 cut(s) 32, 163, 218
MluNI TGGCCA 1 cut(s) 72
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 7 cut(s) 3, 136, 233, 435, 444, 448, 642
Mox20I TGGCCA 1 cut(s) 72
MroI TCCGGA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 72
MseI TTAA 3 cut(s) 435, 492, 723
MslI CAYNNNNRTG 2 cut(s) 78, 735
Msp20I TGGCCA 1 cut(s) 72
MspI CCGG 6 cut(s) 210, 236, 335, 347, 499, 582
MspR9I CCNGG 4 cut(s) 210, 237, 499, 500
Mva1269I GAATGC 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 69, 503
NarI GGCGCC 1 cut(s) 296
NciI CCSGG 4 cut(s) 210, 237, 499, 500
NdeI CATATG 1 cut(s) 417
NdeII GATC 3 cut(s) 96, 178, 202
NlaIII CATG 6 cut(s) 48, 185, 203, 374, 398, 734
NlaIV GGNNCC 3 cut(s) 197, 297, 423
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 2 cut(s) 103, 377
NspI RCATGY 3 cut(s) 48, 374, 734
PaeI GCATGC 1 cut(s) 48
PagI TCATGA 1 cut(s) 181
PciI ACATGT 2 cut(s) 370, 730
PciSI GCTCTTC 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 309
PflFI GACNNNGTC 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 2 cut(s) 88, 716
PleI GAGTC 1 cut(s) 472
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 40
PscI ACATGT 2 cut(s) 370, 730
PspN4I GGNNCC 3 cut(s) 197, 297, 423
PspPI GGNCC 3 cut(s) 403, 421, 665
PsyI GACNNNGTC 1 cut(s) 355
RsaI GTAC 5 cut(s) 140, 247, 488, 569, 608
RsaNI GTAC 5 cut(s) 139, 246, 487, 568, 607
RseI CAYNNNNRTG 2 cut(s) 78, 735
SapI GCTCTTC 1 cut(s) 600
SaqAI TTAA 3 cut(s) 435, 492, 723
SatI GCNGC 2 cut(s) 87, 715
Sau3AI GATC 3 cut(s) 96, 178, 202
Sau96I GGNCC 3 cut(s) 403, 421, 665
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 4 cut(s) 210, 237, 499, 500
SduI GDGCHC 1 cut(s) 595
SetI ASST 7 cut(s) 42, 132, 450, 492, 544, 644, 670
SfaNI GCATC 2 cut(s) 493, 520
SfoI GGCGCC 1 cut(s) 297
SinI GGWCC 2 cut(s) 421, 665
SmaI CCCGGG 1 cut(s) 500
SmiMI CAYNNNNRTG 2 cut(s) 78, 735
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SnaBI TACGTA 1 cut(s) 40
SphI GCATGC 1 cut(s) 48
Sse9I AATT 3 cut(s) 32, 163, 218
SsiI CCGC 4 cut(s) 48, 306, 328, 613
SspDI GGCGCC 1 cut(s) 295
SspI AATATT 1 cut(s) 433
SspMI CTAG 2 cut(s) 131, 560
StyD4I CCNGG 4 cut(s) 208, 235, 497, 498
TaaI ACNGT 4 cut(s) 103, 226, 250, 355
TaiI ACGT 2 cut(s) 42, 544
TaqI TCGA 3 cut(s) 95, 216, 514
TasI AATT 3 cut(s) 32, 163, 218
TatI WGTACW 3 cut(s) 245, 567, 606
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 3 cut(s) 435, 492, 723
Tru9I TTAA 3 cut(s) 435, 492, 723
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 2 cut(s) 103, 377
TseI GCWGC 2 cut(s) 86, 714
Tsp45I GTSAC 2 cut(s) 103, 377
TspDTI ATGAA 1 cut(s) 426
TspMI CCCGGG 1 cut(s) 498
TspRI CASTG 1 cut(s) 106
Tth111I GACNNNGTC 1 cut(s) 355
VpaK11BI GGWCC 2 cut(s) 421, 665
XapI RAATTY 2 cut(s) 163, 218
XceI RCATGY 3 cut(s) 48, 374, 734
XmaI CCCGGG 1 cut(s) 498
XspI CTAG 2 cut(s) 131, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.