AT3G24740

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002431
Physical Location & Seq
Forward (+)
9031078 .. 9033850
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24740.4

Sequence Viewer

Length: 1065 bp
ATGGCTGGTGTAAAGCGAAAATTAAGTACCGAGTCAGATGTTCATGCTCTTCACAAAGAACTGGATGAGGTTTCATGTCCTGTTTGCATGGATCACCCACATAATGCTGTTCTACTGCTGTGCAGCTCTCATGACAAAGGTTGCAGGTCCTACATCTGTGATACCAGTTACCGACATTCCAATTGCCTTGACCGGTTTAAGAAATTGCATTCTGAATCCGCAAATGACCCTACTCCCGAGGCGAACTTGGCTTCAAGGGAACATAATAACGAATCTCTATACGAGCATGGTACAGCCAGTCGAAGCAGTTTTCACCGTGAGTCTGGAAACCGAGGCTCTTCTTGGGATTCCGAATCACTGAGGAGGAGGAGGAGAGTTGAGGAAGAAGTTGAGTCAGAAGATATCACAAATTTGAAATGTCCTCTATGCCGGGGTACTGTTTTAGGATGGAAGGTTGTAGAAGAAGTAAGAACGTATCTTGATCATAAGAACCGAAGTTGCTCCCGAGAATCCTGCTCATTTACTGGCAACTACCAGGATCTGCGCCGCCATGCCAGGAGGACTCACCCAACAACTCGTCCTTCAGACACTGATCCATCAAGAGAGAGAGCTTGGAGGCGGCTTGAGAACCAGAGGGAGTATGGAGATATAGTCAGTGCAATCCGTTCTGCCATGCCCGGTGCTGTTGTAGTAGGAGATTATGTTATAGAAAACGGAGATAGGTTTGCAGGTGAGAGGGAAACGGGAAATGGTGGGAGCGACCTTTGGACCACTTTACTATTGTTTCAGATGATTGGCTCTCTTGACAATGGAGGTTCTAGCGCTAGTGGGAGTGGTGGTGGATCAAGATCCCACAGGTCAAGGGCATGGAGGAATCATCGGCGTTCATCTTCAGATAGGCCGTACCTTTGGGGGGAGAATCTATTGGGTCTACAAGATGAGCGCAATAACAACGATGATGAAGAGTTTCGTTTGCAGAATGATGCAGGCGGTGCTTCTACTCCTGTTCCAAGAAGAAGAAGGAGATTTGGACGTCCTAGATCAAGCGGAAATCATCCGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

354

Amino Acids

40.11

Weight (kDa)

8.68

Isoelectric Point (pI)

71.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 22 - 219 6.5e-72 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AatII GACGTC 1 cut(s) 1036
Acc36I ACCTGC 2 cut(s) 135, 719
AccI GTMKAC 1 cut(s) 931
AccII CGCG 1 cut(s) 1060
AciI CCGC 6 cut(s) 219, 547, 619, 990, 1047, 1058
AclWI GGATC 5 cut(s) 99, 546, 587, 843, 850
AcsI RAATTY 1 cut(s) 409
AcuI CTGAAG 2 cut(s) 567, 876
AcyI GRCGYC 1 cut(s) 1033
AfaI GTAC 4 cut(s) 28, 292, 436, 905
AfeI AGCGCT 1 cut(s) 823
AfiI CCNNNNNNNGG 1 cut(s) 913
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 2 cut(s) 255, 415
AjnI CCWGG 2 cut(s) 534, 554
AluBI AGCT 2 cut(s) 126, 611
AluI AGCT 2 cut(s) 126, 611
AlwI GGATC 5 cut(s) 99, 546, 587, 843, 850
AlwNI CAGNNNCTG 2 cut(s) 541, 590
Ama87I CYCGRG 2 cut(s) 236, 504
Aor51HI AGCGCT 1 cut(s) 823
AoxI GGCC 1 cut(s) 899
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 1 cut(s) 409
AsiGI ACCGGT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 546, 824, 945
AspS9I GGNCC 2 cut(s) 147, 768
AsuC2I CCSGG 2 cut(s) 431, 678
AsuHPI GGTGA 4 cut(s) 86, 305, 557, 743
AvaI CYCGRG 2 cut(s) 236, 504
AvaII GGWCC 2 cut(s) 147, 768
BbvI GCAGC 1 cut(s) 135
BccI CCATC 2 cut(s) 441, 604
BceAI ACGGC 1 cut(s) 886
BcgI CGANNNNNNTGC 2 cut(s) 495, 529
BciT130I CCWGG 2 cut(s) 536, 556
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 2 cut(s) 431, 678
BfaI CTAG 3 cut(s) 819, 825, 1038
BfoI RGCGCY 1 cut(s) 825
BfuAI ACCTGC 2 cut(s) 135, 719
BisI GCNGC 3 cut(s) 124, 547, 620
BlsI GCNGC 3 cut(s) 125, 548, 621
Bme1390I CCNGG 4 cut(s) 431, 536, 556, 678
Bme18I GGWCC 2 cut(s) 147, 768
BmeT110I CYCGRG 2 cut(s) 236, 504
BmgT120I GGNCC 2 cut(s) 147, 768
BmrFI CCNGG 4 cut(s) 431, 536, 556, 678
BmsI GCATC 1 cut(s) 973
BpuEI CTTGAG 1 cut(s) 644
BpuMI CCSGG 2 cut(s) 431, 678
BsaBI GATNNNNATC 1 cut(s) 847
BsaHI GRCGYC 1 cut(s) 1033
BsaJI CCNNGG 3 cut(s) 237, 331, 430
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 6 cut(s) 636, 666, 687, 717, 823, 853
Bsc4I CCNNNNNNNGG 1 cut(s) 913
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 4 cut(s) 66, 165, 297, 529
Bse8I GATNNNNATC 1 cut(s) 847
BseBI CCWGG 2 cut(s) 536, 556
BseDI CCNNGG 3 cut(s) 237, 331, 430
BseGI GGATG 3 cut(s) 70, 452, 1054
BseJI GATNNNNATC 1 cut(s) 847
BseLI CCNNNNNNNGG 1 cut(s) 913
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 4 cut(s) 66, 165, 297, 529
BseRI GAGGAG 4 cut(s) 376, 379, 382, 385
BseXI GCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 1060
BshFI GGCC 1 cut(s) 901
BshTI ACCGGT 1 cut(s) 192
BsiHKCI CYCGRG 2 cut(s) 236, 504
BsiSI CCGG 3 cut(s) 193, 430, 678
BslI CCNNNNNNNGG 1 cut(s) 913
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 901
BsoBI CYCGRG 2 cut(s) 236, 504
Bsp143I GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
BspACI CCGC 6 cut(s) 219, 547, 619, 990, 1047, 1058
BspANI GGCC 1 cut(s) 901
BspCNI CTCAG 1 cut(s) 351
BspFNI CGCG 1 cut(s) 1060
BspHI TCATGA 1 cut(s) 130
BspMI ACCTGC 2 cut(s) 135, 719
BspPI GGATC 5 cut(s) 99, 546, 587, 843, 850
BspQI GCTCTTC 2 cut(s) 54, 343
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 4 cut(s) 66, 165, 297, 529
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 3 cut(s) 237, 331, 430
BssMI GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
BssNI GRCGYC 1 cut(s) 1033
Bst2UI CCWGG 2 cut(s) 536, 556
Bst4CI ACNGT 2 cut(s) 317, 439
Bst6I CTCTTC 3 cut(s) 54, 343, 957
BstACI GRCGYC 1 cut(s) 1033
BstAPI GCANNNNNTGC 1 cut(s) 992
BstC8I GCNNGC 1 cut(s) 988
BstDEI CTNAG 1 cut(s) 359
BstF5I GGATG 3 cut(s) 70, 452, 1054
BstFNI CGCG 1 cut(s) 1060
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 3 cut(s) 546, 824, 945
BstKTI GATC 7 cut(s) 94, 484, 541, 595, 845, 851, 1043
BstMBI GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
BstMWI GCNNNNNNNGC 2 cut(s) 248, 992
BstNI CCWGG 2 cut(s) 536, 556
BstSCI CCNGG 4 cut(s) 429, 534, 554, 676
BstUI CGCG 1 cut(s) 1060
BstV1I GCAGC 1 cut(s) 135
BstX2I RGATCY 2 cut(s) 538, 848
BstYI RGATCY 2 cut(s) 538, 848
BsuRI GGCC 1 cut(s) 901
BtsCI GGATG 3 cut(s) 70, 452, 1054
BtsIMutI CAGTG 3 cut(s) 356, 588, 661
BveI ACCTGC 2 cut(s) 135, 719
Cac8I GCNNGC 1 cut(s) 988
CaiI CAGNNNCTG 2 cut(s) 541, 590
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 546, 824, 945
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 2 cut(s) 147, 768
Csp6I GTAC 4 cut(s) 27, 291, 435, 904
CspAI ACCGGT 1 cut(s) 192
CviAII CATG 8 cut(s) 44, 75, 88, 131, 287, 551, 673, 867
CviJI RGCY 9 cut(s) 5, 126, 251, 296, 336, 611, 622, 798, 901
CviKI_1 RGCY 9 cut(s) 5, 126, 251, 296, 336, 611, 622, 798, 901
CviQI GTAC 4 cut(s) 27, 291, 435, 904
DdeI CTNAG 1 cut(s) 359
DpnI GATC 7 cut(s) 93, 483, 540, 594, 844, 850, 1042
DpnII GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
Eam1104I CTCTTC 3 cut(s) 54, 343, 957
EarI CTCTTC 3 cut(s) 54, 343, 957
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 147, 768
Eco47III AGCGCT 1 cut(s) 823
Eco57I CTGAAG 2 cut(s) 567, 876
Eco88I CYCGRG 2 cut(s) 236, 504
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 534, 554
EcoRV GATATC 1 cut(s) 403
FaeI CATG 8 cut(s) 47, 78, 91, 134, 290, 554, 676, 870
FatI CATG 8 cut(s) 43, 74, 87, 130, 286, 550, 672, 866
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 1 cut(s) 931
Fnu4HI GCNGC 3 cut(s) 124, 547, 620
FokI GGATG 3 cut(s) 77, 459, 1041
Fsp4HI GCNGC 3 cut(s) 124, 547, 620
FspBI CTAG 3 cut(s) 819, 825, 1038
GlaI GCGC 3 cut(s) 545, 823, 944
GluI GCNGC 3 cut(s) 124, 547, 620
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 1 cut(s) 901
HapII CCGG 3 cut(s) 193, 430, 678
HhaI GCGC 3 cut(s) 546, 824, 945
Hin1I GRCGYC 1 cut(s) 1033
Hin1II CATG 8 cut(s) 47, 78, 91, 134, 290, 554, 676, 870
Hin6I GCGC 3 cut(s) 544, 822, 943
HinP1I GCGC 3 cut(s) 544, 822, 943
HpaII CCGG 3 cut(s) 193, 430, 678
HphI GGTGA 4 cut(s) 86, 305, 557, 743
Hpy166II GTNNAC 1 cut(s) 932
Hpy188I TCNGA 7 cut(s) 37, 214, 352, 397, 586, 789, 895
Hpy188III TCNNGA 8 cut(s) 131, 236, 324, 479, 504, 600, 803, 846
Hpy8I GTNNAC 1 cut(s) 932
HpyAV CCTTC 3 cut(s) 445, 591, 1014
HpyCH4III ACNGT 2 cut(s) 317, 439
HpyCH4IV ACGT 2 cut(s) 473, 1033
HpyCH4V TGCA 8 cut(s) 87, 123, 144, 208, 659, 728, 976, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 992
HpyF3I CTNAG 1 cut(s) 359
HpySE526I ACGT 2 cut(s) 473, 1033
Hsp92I GRCGYC 1 cut(s) 1033
Hsp92II CATG 8 cut(s) 47, 78, 91, 134, 290, 554, 676, 870
HspAI GCGC 3 cut(s) 544, 822, 943
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
LguI GCTCTTC 2 cut(s) 54, 343
LmnI GCTCC 2 cut(s) 506, 756
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 973
MaeI CTAG 3 cut(s) 819, 825, 1038
MaeII ACGT 2 cut(s) 473, 1033
MaeIII GTNAC 1 cut(s) 167
MalI GATC 7 cut(s) 93, 483, 540, 594, 844, 850, 1042
MboI GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
MboII GAAGA 9 cut(s) 41, 330, 395, 410, 473, 882, 974, 1026, 1029
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 2 cut(s) 538, 848
MluCI AATT 4 cut(s) 20, 181, 203, 409
MlyI GAGTC 4 cut(s) 41, 329, 401, 556
MroXI GAANNNNTTC 1 cut(s) 966
MseI TTAA 2 cut(s) 23, 198
MspI CCGG 3 cut(s) 193, 430, 678
MspR9I CCNGG 4 cut(s) 431, 536, 556, 678
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 2 cut(s) 536, 556
MvnI CGCG 1 cut(s) 1060
MwoI GCNNNNNNNGC 2 cut(s) 248, 992
NciI CCSGG 2 cut(s) 431, 678
NdeII GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
NlaIII CATG 8 cut(s) 47, 78, 91, 134, 290, 554, 676, 870
PagI TCATGA 1 cut(s) 130
PaqCI CACCTGC 1 cut(s) 719
PciSI GCTCTTC 2 cut(s) 54, 343
PctI GAATGC 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 7 cut(s) 215, 272, 347, 353, 509, 874, 919
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 3 cut(s) 125, 548, 621
PleI GAGTC 4 cut(s) 40, 328, 400, 556
PpsI GAGTC 4 cut(s) 40, 328, 400, 556
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 534, 554
PspGI CCWGG 2 cut(s) 534, 554
PspPI GGNCC 2 cut(s) 147, 768
PspPPI RGGWCCY 1 cut(s) 147
PstNI CAGNNNCTG 2 cut(s) 541, 590
PsuI RGATCY 2 cut(s) 538, 848
RsaI GTAC 4 cut(s) 28, 292, 436, 905
RsaNI GTAC 4 cut(s) 27, 291, 435, 904
SapI GCTCTTC 2 cut(s) 54, 343
SaqAI TTAA 2 cut(s) 23, 198
SatI GCNGC 3 cut(s) 124, 547, 620
Sau3AI GATC 7 cut(s) 91, 481, 538, 592, 842, 848, 1040
Sau96I GGNCC 2 cut(s) 147, 768
SchI GAGTC 4 cut(s) 41, 329, 401, 556
ScrFI CCNGG 4 cut(s) 431, 536, 556, 678
SfaNI GCATC 1 cut(s) 973
SinI GGWCC 2 cut(s) 147, 768
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 4 cut(s) 20, 181, 203, 409
SsiI CCGC 6 cut(s) 219, 547, 619, 990, 1047, 1058
SspMI CTAG 3 cut(s) 819, 825, 1038
StyD4I CCNGG 4 cut(s) 429, 534, 554, 676
TaaI ACNGT 2 cut(s) 317, 439
TaiI ACGT 2 cut(s) 476, 1036
TaqI TCGA 1 cut(s) 301
TasI AATT 4 cut(s) 20, 181, 203, 409
TauI GCSGC 2 cut(s) 549, 622
TfiI GAWTC 7 cut(s) 215, 272, 347, 353, 509, 874, 919
Tru1I TTAA 2 cut(s) 23, 198
Tru9I TTAA 2 cut(s) 23, 198
TscAI CASTG 3 cut(s) 363, 595, 661
TseI GCWGC 1 cut(s) 123
TspDTI ATGAA 4 cut(s) 32, 63, 876, 975
TspGWI ACGGA 2 cut(s) 653, 729
TspRI CASTG 3 cut(s) 363, 595, 661
VpaK11BI GGWCC 2 cut(s) 147, 768
XapI RAATTY 1 cut(s) 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 931
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 3 cut(s) 819, 825, 1038
ZraI GACGTC 1 cut(s) 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.