Rorug06G0172500

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002431
Physical Location & Seq
Forward (+)
25486309 .. 25488298
1990 bp
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UTR
Exon/CDS
Intron
Rorug06G0172500.1

Sequence Viewer

Length: 321 bp
ATGGTTGTTCCTCCACCTGTCAGGCCACCTACTATTACTAAGTTTCTGAAGCCTTATGTCCTGAAAATGCACTTTACAAATAAATATGTAAGTGCCCAGGTAGTCCACTCACCAACTGCCACTGTAGCCTCTTCCGCAAGCTCGCAGGAAAAAGCCTTGAGAGAGAGCCTGGAATCGCATAGGGATGTTGCTGCTGCTGCAAAGATTGGGAAGATATTAGGGGAGCGCCTGCTGCTCAAGAATATCCCTGCTGTATCAGTCCACTTGAAGAGAGAACAGAAATATCATGAGTGGTGGACTGGCGATGTTGGCTTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

106

Amino Acids

11.85

Weight (kDa)

9.93

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 20 - 96 4.3e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 68
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 2 cut(s) 96, 168
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 4 cut(s) 191, 194, 197, 232
AspLEI GCGC 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 97
BbvI GCAGC 4 cut(s) 178, 181, 184, 219
BciT130I CCWGG 2 cut(s) 98, 170
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 229
BisI GCNGC 4 cut(s) 192, 195, 198, 233
BlsI GCNGC 4 cut(s) 193, 196, 199, 234
Bme1390I CCNGG 2 cut(s) 98, 170
BmrFI CCNGG 2 cut(s) 98, 170
BpuEI CTTGAG 2 cut(s) 178, 221
BsaJI CCNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 2 cut(s) 98, 170
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 190
BseNI ACTGG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 4 cut(s) 178, 181, 184, 219
BshFI GGCC 1 cut(s) 25
BsnI GGCC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 97
BspACI CCGC 1 cut(s) 135
BspANI GGCC 1 cut(s) 25
BspHI TCATGA 1 cut(s) 286
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 98, 170
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 136, 263
BstC8I GCNNGC 3 cut(s) 139, 143, 230
BstDEI CTNAG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 190
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstMWI GCNNNNNNNGC 5 cut(s) 125, 134, 197, 232, 309
BstNI CCWGG 2 cut(s) 98, 170
BstSCI CCNGG 2 cut(s) 96, 168
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 4 cut(s) 178, 181, 184, 219
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 120, 316
Cac8I GCNNGC 3 cut(s) 139, 143, 230
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 228
CviAII CATG 1 cut(s) 287
CviJI RGCY 7 cut(s) 25, 52, 128, 141, 155, 168, 312
CviKI_1 RGCY 7 cut(s) 25, 52, 128, 141, 155, 168, 312
DdeI CTNAG 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 136, 263
EarI CTCTTC 2 cut(s) 136, 263
Eco57I CTGAAG 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 96, 168
FaeI CATG 1 cut(s) 290
FaiI YATR 4 cut(s) 57, 87, 180, 288
FatI CATG 1 cut(s) 286
Fnu4HI GCNGC 4 cut(s) 192, 195, 198, 233
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 4 cut(s) 192, 195, 198, 233
GlaI GCGC 1 cut(s) 227
GluI GCNGC 4 cut(s) 192, 195, 198, 233
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 1 cut(s) 25
HhaI GCGC 1 cut(s) 228
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 1 cut(s) 173
HphI GGTGA 1 cut(s) 102
Hpy166II GTNNAC 3 cut(s) 106, 262, 297
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 3 cut(s) 61, 238, 287
Hpy8I GTNNAC 3 cut(s) 106, 262, 297
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 70, 200
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 134, 197, 232, 309
HpyF3I CTNAG 1 cut(s) 39
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 1 cut(s) 226
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 4 cut(s) 178, 181, 184, 219
MboII GAAGA 3 cut(s) 123, 223, 280
MhlI GDGCHC 1 cut(s) 97
MnlI CCTC 2 cut(s) 21, 139
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 98, 170
MvaI CCWGG 2 cut(s) 98, 170
MwoI GCNNNNNNNGC 5 cut(s) 125, 134, 197, 232, 309
NlaIII CATG 1 cut(s) 290
PagI TCATGA 1 cut(s) 286
PfeI GAWTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 193, 196, 199, 234
Psp6I CCWGG 2 cut(s) 96, 168
PspGI CCWGG 2 cut(s) 96, 168
RseI CAYNNNNRTG 1 cut(s) 183
SatI GCNGC 4 cut(s) 192, 195, 198, 233
ScrFI CCNGG 2 cut(s) 98, 170
SduI GDGCHC 1 cut(s) 97
SetI ASST 4 cut(s) 19, 31, 102, 143
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 2 cut(s) 157, 236
SmoI CTYRAG 2 cut(s) 157, 236
SsiI CCGC 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 96, 168
TaaI ACNGT 1 cut(s) 124
TfiI GAWTC 1 cut(s) 173
TscAI CASTG 1 cut(s) 127
TseI GCWGC 4 cut(s) 191, 194, 197, 232
TspRI CASTG 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.