FvH4_2g19370

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002431
Physical Location & Seq
Reverse (-)
16472993 .. 16476727
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19370.t7

Sequence Viewer

Length: 1158 bp
ATGGCTGGTGTGAAACGAAGAATAGATACTGGTTCAGAAATTCGTGCTCTTTACAAAGAACTGGATGCTGTTTCATGCCCCATCTGCATGGACCATCCACATAATGCAGTTCTCCTCCTTTGCAGCTCTCATGACAAGGGTTGCAGATCTTACATTTGTGATACTAGTTACAGGCACTCGAATTGCCTGGACCGTTTTAAAAAGTTGAGGGAAAATAATACAAACAGTCAAAGTTTAGTCAGTTCTTTACCTACAAACCATCATGGCTCTCATAATACTCCTGATATGGCATTCGGAACAGATTTGAATGAAGCTAATGGAAGTCCTAACTTGATTGAAGGCAATGCTGTAACATCTGCTAATATACCTGGACAGCCACAAGAGCGTGTTATTCAGGATCTAAATATGCCACTTCTACCGGAAGAACTTATGGGAGTGGCTGATTCTGAGTCATTTCAGGAAAGGGTTGAGCATGGTGAATTAGATGTGGAAAATTCATCAGAGTCAAATTTGAGCTTGAAATGTCCTTTGTGCCGAGGAGCCATCCTCGGCTGGGAGGTTGTGGAAGATTGCAGAAAGTATCTTAATCTGAAGAAGCGAAGTTGTTCTCGTGAAGCATGCTCTTTTTCTGGTAACTACCAGGAGTTGCGTCGGCATGCTAGGAGAGTTCATCCTGCCACTCGGCCCTCAGACATTGACCCATCTAGAGAGCGAGCATGGAGACACCTTGAGCACCAGAGAGAATTTGGTGATGTTGTTAGTGCTATTCATTCAGCCATACCAGGAGCTGTTGTGGTAGGGGATTATGTGATTGAAAATGGAGATAGGCTAGGGGGTGGAGGTGAAAGTGGTACTGGTGAAGCTAATGGTCCTTGGTGGACTACCATGTTTTTGTTTCAAATGATTGGGTCAGCTGATCGTGGTGGGGAACCAAGGGCAAGGGCAAGGGCTTGGCCTAGACATCGGCGATCGGCGGGAGCTTTATCTGAACGGCGTTTACTTTGGGGTGAGAATTTATTGGGTCTTCAAGATGATGACGAAGATGATGAGGATGACGGTGAGGAGGACATTCTCATGCTGAATGACAGAGATGTATCTCCAATTCCTAGAAGGCGCCGGCGTTTGACACGTTCTAGGTCAGTCGAAGACCGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

386

Amino Acids

43.14

Weight (kDa)

5.74

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 23 - 254 5.9e-72 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1113
AciI CCGC 1 cut(s) 974
AclWI GGATC 1 cut(s) 405
AcsI RAATTY 5 cut(s) 39, 493, 508, 743, 1012
AcuI CTGAAG 1 cut(s) 611
AcyI GRCGYC 1 cut(s) 1114
AfaI GTAC 1 cut(s) 853
AfiI CCNNNNNNNGG 1 cut(s) 553
AflIII ACRYGT 1 cut(s) 1127
AgsI TTSAA 6 cut(s) 307, 338, 520, 815, 899, 1028
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 4 cut(s) 186, 367, 639, 781
AluBI AGCT 7 cut(s) 126, 314, 516, 788, 863, 914, 980
AluI AGCT 7 cut(s) 126, 314, 516, 788, 863, 914, 980
Alw21I GWGCWC 2 cut(s) 49, 735
Alw26I GTCTC 1 cut(s) 715
AlwI GGATC 1 cut(s) 405
AlwNI CAGNNNCTG 1 cut(s) 788
AoxI GGCC 2 cut(s) 683, 953
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 5 cut(s) 39, 493, 508, 743, 1012
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 1 cut(s) 1116
AspS9I GGNCC 4 cut(s) 91, 190, 684, 869
AsuHPI GGTGA 6 cut(s) 488, 761, 854, 869, 1019, 1070
AvaII GGWCC 3 cut(s) 91, 190, 869
BanI GGYRCC 1 cut(s) 1113
BarI GAAGNNNNNNTAC 2 cut(s) 10, 42
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 2 cut(s) 1016, 1152
Bbv12I GWGCWC 2 cut(s) 49, 735
BbvI GCAGC 1 cut(s) 135
BccI CCATC 5 cut(s) 89, 102, 267, 551, 709
BceAI ACGGC 1 cut(s) 1007
BciT130I CCWGG 4 cut(s) 188, 369, 641, 783
BcoDI GTCTC 1 cut(s) 715
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 7 cut(s) 165, 660, 705, 830, 957, 1107, 1134
BfoI RGCGCY 1 cut(s) 1117
BglII AGATCT 1 cut(s) 146
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 4 cut(s) 188, 369, 641, 783
Bme18I GGWCC 3 cut(s) 91, 190, 869
BmgT120I GGNCC 4 cut(s) 91, 190, 684, 869
BmiI GGNNCC 3 cut(s) 541, 930, 1115
BmrFI CCNGG 4 cut(s) 188, 369, 641, 783
BmsI GCATC 1 cut(s) 55
BpiI GAAGAC 2 cut(s) 1016, 1152
BplI GAGNNNNNCTC 2 cut(s) 531, 563
BpuEI CTTGAG 1 cut(s) 749
BsaHI GRCGYC 1 cut(s) 1114
BsaJI CCNNGG 4 cut(s) 535, 547, 872, 932
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse118I RCCGGY 1 cut(s) 1116
Bse1I ACTGG 3 cut(s) 34, 66, 859
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 4 cut(s) 188, 369, 641, 783
BseDI CCNNGG 4 cut(s) 535, 547, 872, 932
BseGI GGATG 5 cut(s) 70, 94, 543, 670, 1057
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 2 cut(s) 438, 702
BseNI ACTGG 3 cut(s) 34, 66, 859
BseRI GAGGAG 3 cut(s) 104, 552, 1076
BseXI GCAGC 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 552
Bsh1285I CGRYCG 1 cut(s) 971
BshFI GGCC 2 cut(s) 685, 955
BshNI GGYRCC 1 cut(s) 1113
BsiEI CGRYCG 1 cut(s) 971
BsiHKAI GWGCWC 2 cut(s) 49, 735
BsiSI CCGG 2 cut(s) 419, 1117
BslFI GGGAC 1 cut(s) 1137
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 1 cut(s) 715
BsmFI GGGAC 1 cut(s) 1137
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 2 cut(s) 685, 955
Bsp1286I GDGCHC 2 cut(s) 49, 735
Bsp143I GATC 4 cut(s) 146, 397, 916, 968
BspACI CCGC 1 cut(s) 974
BspANI GGCC 2 cut(s) 685, 955
BspCNI CTCAG 2 cut(s) 439, 701
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 541, 930, 1115
BspPI GGATC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 1113
BsrDI GCAATG 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 1116
BsrI ACTGG 3 cut(s) 34, 66, 859
BssAI RCCGGY 1 cut(s) 1116
BssECI CCNNGG 4 cut(s) 535, 547, 872, 932
BssMI GATC 4 cut(s) 146, 397, 916, 968
BssNI GRCGYC 1 cut(s) 1114
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 872, 932
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 4 cut(s) 188, 369, 641, 783
Bst4CI ACNGT 4 cut(s) 194, 227, 1058, 1151
BstACI GRCGYC 1 cut(s) 1114
BstC8I GCNNGC 4 cut(s) 619, 657, 714, 1118
BstDEI CTNAG 2 cut(s) 447, 688
BstF5I GGATG 5 cut(s) 70, 94, 543, 670, 1057
BstH2I RGCGCY 1 cut(s) 1117
BstHHI GCGC 1 cut(s) 1116
BstKTI GATC 4 cut(s) 149, 400, 919, 971
BstMAI GTCTC 1 cut(s) 715
BstMBI GATC 4 cut(s) 146, 397, 916, 968
BstMCI CGRYCG 1 cut(s) 971
BstMWI GCNNNNNNNGC 2 cut(s) 84, 382
BstNI CCWGG 4 cut(s) 188, 369, 641, 783
BstNSI RCATGY 2 cut(s) 621, 659
BstSCI CCNGG 4 cut(s) 186, 367, 639, 781
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 2 cut(s) 1016, 1152
BstX2I RGATCY 2 cut(s) 146, 397
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 2 cut(s) 146, 397
BsuRI GGCC 2 cut(s) 685, 955
BtsCI GGATG 5 cut(s) 70, 94, 543, 670, 1057
Cac8I GCNNGC 4 cut(s) 619, 657, 714, 1118
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 130
CfoI GCGC 1 cut(s) 1116
Cfr10I RCCGGY 1 cut(s) 1116
Cfr13I GGNCC 4 cut(s) 91, 190, 684, 869
CseI GACGC 1 cut(s) 638
Csp6I GTAC 1 cut(s) 852
CviQI GTAC 1 cut(s) 852
DdeI CTNAG 2 cut(s) 447, 688
DinI GGCGCC 1 cut(s) 1115
DpnI GATC 4 cut(s) 148, 399, 918, 970
DpnII GATC 4 cut(s) 146, 397, 916, 968
DraI TTTAAA 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 872, 932
Eco47I GGWCC 3 cut(s) 91, 190, 869
Eco57I CTGAAG 1 cut(s) 611
EcoRII CCWGG 4 cut(s) 186, 367, 639, 781
EcoT14I CCWWGG 2 cut(s) 872, 932
EgeI GGCGCC 1 cut(s) 1115
EheI GGCGCC 1 cut(s) 1115
ErhI CCWWGG 2 cut(s) 872, 932
FaqI GGGAC 1 cut(s) 1137
FauI CCCGC 1 cut(s) 967
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 5 cut(s) 77, 81, 530, 657, 1064
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 7 cut(s) 165, 660, 705, 830, 957, 1107, 1134
GlaI GCGC 1 cut(s) 1115
GluI GCNGC 1 cut(s) 124
GsaI CCCAGC 1 cut(s) 556
HaeII RGCGCY 1 cut(s) 1117
HaeIII GGCC 2 cut(s) 685, 955
HapII CCGG 2 cut(s) 419, 1117
HgaI GACGC 1 cut(s) 638
HhaI GCGC 1 cut(s) 1116
Hin1I GRCGYC 1 cut(s) 1114
Hin6I GCGC 1 cut(s) 1114
HinP1I GCGC 1 cut(s) 1114
HinfI GANTC 3 cut(s) 443, 449, 503
HpaII CCGG 2 cut(s) 419, 1117
HphI GGTGA 6 cut(s) 488, 761, 854, 869, 1019, 1070
Hpy166II GTNNAC 2 cut(s) 879, 998
Hpy188I TCNGA 7 cut(s) 37, 296, 448, 502, 591, 691, 988
Hpy188III TCNNGA 7 cut(s) 131, 281, 395, 458, 611, 705, 1028
Hpy8I GTNNAC 2 cut(s) 879, 998
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 2 cut(s) 332, 1104
HpyCH4III ACNGT 4 cut(s) 194, 227, 1058, 1151
HpyCH4IV ACGT 1 cut(s) 1129
HpyCH4V TGCA 5 cut(s) 87, 107, 123, 144, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 382
HpyF3I CTNAG 2 cut(s) 447, 688
HpySE526I ACGT 1 cut(s) 1129
Hsp92I GRCGYC 1 cut(s) 1114
HspAI GCGC 1 cut(s) 1114
KasI GGCGCC 1 cut(s) 1113
KroI GCCGGC 1 cut(s) 1116
KroNI GCCGGC 1 cut(s) 1118
Kzo9I GATC 4 cut(s) 146, 397, 916, 968
LmnI GCTCC 3 cut(s) 539, 785, 977
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 55
MaeI CTAG 7 cut(s) 165, 660, 705, 830, 957, 1107, 1134
MaeII ACGT 1 cut(s) 1129
MaeIII GTNAC 3 cut(s) 167, 349, 632
MalI GATC 4 cut(s) 148, 399, 918, 970
MboI GATC 4 cut(s) 146, 397, 916, 968
MboII GAAGA 7 cut(s) 30, 434, 578, 604, 1016, 1052, 1157
MflI RGATCY 2 cut(s) 146, 397
MhlI GDGCHC 2 cut(s) 49, 735
MluCI AATT 8 cut(s) 39, 181, 479, 493, 508, 743, 1012, 1101
Mly113I GGCGCC 1 cut(s) 1114
MlyI GAGTC 2 cut(s) 458, 512
MreI CGCCGGCG 1 cut(s) 1116
MroNI GCCGGC 1 cut(s) 1116
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 2 cut(s) 198, 585
MslI CAYNNNNRTG 2 cut(s) 86, 1073
MspA1I CMGCKG 1 cut(s) 914
MspI CCGG 2 cut(s) 419, 1117
MspR9I CCNGG 4 cut(s) 188, 369, 641, 783
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 4 cut(s) 188, 369, 641, 783
MwoI GCNNNNNNNGC 2 cut(s) 84, 382
NaeI GCCGGC 1 cut(s) 1118
NarI GGCGCC 1 cut(s) 1114
NdeII GATC 4 cut(s) 146, 397, 916, 968
NgoMIV GCCGGC 1 cut(s) 1116
NlaIV GGNNCC 3 cut(s) 541, 930, 1115
NmeAIII GCCGAG 3 cut(s) 528, 560, 661
NspI RCATGY 2 cut(s) 621, 659
PaeI GCATGC 2 cut(s) 621, 659
PagI TCATGA 1 cut(s) 130
PctI GAATGC 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 1118
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 443
PkrI GCNGC 1 cut(s) 125
Ple19I CGATCG 1 cut(s) 971
PleI GAGTC 2 cut(s) 457, 511
PluTI GGCGCC 1 cut(s) 1117
PpsI GAGTC 2 cut(s) 457, 511
Psp6I CCWGG 4 cut(s) 186, 367, 639, 781
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 4 cut(s) 186, 367, 639, 781
PspN4I GGNNCC 3 cut(s) 541, 930, 1115
PspPI GGNCC 4 cut(s) 91, 190, 684, 869
PsrI GAACNNNNNNTAC 2 cut(s) 981, 1013
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 2 cut(s) 146, 397
PvuI CGATCG 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 1 cut(s) 853
RsaNI GTAC 1 cut(s) 852
RseI CAYNNNNRTG 2 cut(s) 86, 1073
SaqAI TTAA 2 cut(s) 198, 585
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 4 cut(s) 146, 397, 916, 968
Sau96I GGNCC 4 cut(s) 91, 190, 684, 869
SchI GAGTC 2 cut(s) 458, 512
ScrFI CCNGG 4 cut(s) 188, 369, 641, 783
SduI GDGCHC 2 cut(s) 49, 735
SfaNI GCATC 1 cut(s) 55
SfoI GGCGCC 1 cut(s) 1115
SgrAI CRCCGGYG 1 cut(s) 1116
SinI GGWCC 3 cut(s) 91, 190, 869
SmiMI CAYNNNNRTG 2 cut(s) 86, 1073
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 164
SphI GCATGC 2 cut(s) 621, 659
Sse9I AATT 8 cut(s) 39, 181, 479, 493, 508, 743, 1012, 1101
SsiI CCGC 1 cut(s) 974
SspDI GGCGCC 1 cut(s) 1113
SspMI CTAG 7 cut(s) 165, 660, 705, 830, 957, 1107, 1134
StyD4I CCNGG 4 cut(s) 186, 367, 639, 781
StyI CCWWGG 2 cut(s) 872, 932
TaaI ACNGT 4 cut(s) 194, 227, 1058, 1151
TaiI ACGT 1 cut(s) 1132
TaqI TCGA 2 cut(s) 179, 1143
TasI AATT 8 cut(s) 39, 181, 479, 493, 508, 743, 1012, 1101
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 2 cut(s) 198, 585
Tru9I TTAA 2 cut(s) 198, 585
TseI GCWGC 1 cut(s) 123
TspDTI ATGAA 5 cut(s) 63, 324, 486, 659, 758
VpaK11BI GGWCC 3 cut(s) 91, 190, 869
XapI RAATTY 5 cut(s) 39, 493, 508, 743, 1012
XbaI TCTAGA 1 cut(s) 704
XceI RCATGY 2 cut(s) 621, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 7 cut(s) 165, 660, 705, 830, 957, 1107, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.