MD10G1166800.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002431
Physical Location & Seq
Reverse (-)
25707922 .. 25711484
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1166800.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCTGGTGTGAAACGAAGATTAGATACTGATTCAGATATTCATGCTCTTAACAAAGAACTGGATGCCGTTTCGTGCCCCATCTGCATGGACCATCCACATAATGCCGTTCTCCTGCTTTGTAGCTCGCATGATAAAGGTTGCAGATCATATATATGTGATACAAGTTACAGGCATTCAAATTGCCTGGACCGTTTTAAAAAGTTGAGGGAAAATACTAGGAACAGTCCAACTCTACCCAGTCCTTTACCTATAAACCGTTATGGTCCTAGCATTACTTCTGATCTGAACATCCCTCTGAGAATAGACTCAAATGAAGCTAATGAAAGTCACAACCTGATTGAAAGCAATTCTGTATTATCTGTTAATTTACCTGGACTGCGACAACAAAATGTTATTCAAGATTTAAATAGGCCTTTTGAAGCACAACCAGAAGGTTTTATGGAAGTGGTTGATTCTGAGTCATTTCAGGAAAGGGCTGAACATGATGATTTGGATGGGGAAATTTCACCGGAGTCACAATTGAGCTTGAAATGCCCTATGTGCCGTGGAGCCATTCTTGGATGGGAGGTTGTAGAAGATGCCAGAAAATATCTTAACCTGAAGAAGCGAAGCTGTTCTCGGGAATCATGCTCATTTTCTGGAAACTACCAGGAGTTGCGCCGGCATGCCAGGAGAGTTCACCCAACCACTAGACCCTCTGACATTGACCCGTCTAGAGAACGAGCCTGGCGACACCTTGAGCACCAGAGAGAATTTGGTGATGTTGCAAGTGCTATTCATTCAGCCATGCCAGGTGCTGTTGTGGTTGGGGACTATGTTATTGAAAATGGAGATAGGCTAGCGGGTGGAGGGGAAAGTGGTGTAGTTGAAGCTAATGGACCTTGGTGGACAACTCTGTTTTTGTTTCAGATGATTGGCTCAGCTGACCGTGCTGGGGAACCAAGGGCTCGATCGAGGCCTTGGACAAGACATCGACGGTCAGCAGGTGCTTTAAATGAACGCCGGTTTCTTTGGGGTGAGAATTTGTTAGGTCTTCAGGATGATGACGATGATGACGATGATGAGGACAATGATGATGACAACATGCCTATGATTAATCACAGAGATTTGTCTCCAATCCCCAGAAGGCGTCGGCGTTTAACACGCTTGAGGTCCGATGAGGATCGACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

392

Amino Acids

44.48

Weight (kDa)

5.66

Isoelectric Point (pI)

66.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 23 - 258 2.5e-68 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 977
Acc36I ACCTGC 1 cut(s) 977
AciI CCGC 1 cut(s) 845
AclWI GGATC 1 cut(s) 1173
AcsI RAATTY 3 cut(s) 504, 755, 1024
AcuI CTGAAG 2 cut(s) 623, 1022
AcyI GRCGYC 1 cut(s) 1132
AfiI CCNNNNNNNGG 1 cut(s) 937
AgsI TTSAA 7 cut(s) 180, 344, 401, 422, 532, 827, 872
AjnI CCWGG 6 cut(s) 186, 373, 651, 671, 728, 793
AluBI AGCT 6 cut(s) 126, 320, 528, 615, 875, 926
AluI AGCT 6 cut(s) 126, 320, 528, 615, 875, 926
Alw21I GWGCWC 1 cut(s) 747
Alw26I GTCTC 1 cut(s) 1119
AlwI GGATC 1 cut(s) 1173
AlwNI CAGNNNCTG 1 cut(s) 800
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 2 cut(s) 413, 959
ApoI RAATTY 3 cut(s) 504, 755, 1024
AseI ATTAAT 1 cut(s) 1098
Asp700I GAANNNNTTC 1 cut(s) 616
AspLEI GCGC 1 cut(s) 663
AspS9I GGNCC 5 cut(s) 91, 190, 266, 881, 1155
AsuHPI GGTGA 4 cut(s) 501, 674, 773, 1031
AsuNHI GCTAGC 1 cut(s) 841
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 5 cut(s) 91, 190, 266, 881, 1155
BaeGI GKGCMC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 952
BarI GAAGNNNNNNTAC 2 cut(s) 10, 42
BbsI GAAGAC 1 cut(s) 1028
Bbv12I GWGCWC 1 cut(s) 747
BccI CCATC 4 cut(s) 89, 102, 491, 558
BceAI ACGGC 3 cut(s) 53, 92, 531
BciT130I CCWGG 6 cut(s) 188, 375, 653, 673, 730, 795
BcoDI GTCTC 1 cut(s) 1119
BfaI CTAG 5 cut(s) 219, 270, 693, 717, 842
BfuAI ACCTGC 1 cut(s) 977
BlpI GCTNAGC 1 cut(s) 922
Bme1390I CCNGG 6 cut(s) 188, 375, 653, 673, 730, 795
Bme18I GGWCC 5 cut(s) 91, 190, 266, 881, 1155
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 5 cut(s) 91, 190, 266, 881, 1155
BmiI GGNNCC 2 cut(s) 553, 942
BmrFI CCNGG 6 cut(s) 188, 375, 653, 673, 730, 795
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 55, 571
BmtI GCTAGC 1 cut(s) 845
BmuI ACTGGG 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 1028
Bpu1102I GCTNAGC 1 cut(s) 922
BpuEI CTTGAG 2 cut(s) 761, 1171
BsaBI GATNNNNATC 1 cut(s) 1164
BsaHI GRCGYC 1 cut(s) 1132
BsaJI CCNNGG 4 cut(s) 547, 884, 944, 962
BsaWI WCCGGW 1 cut(s) 511
Bsc4I CCNNNNNNNGG 1 cut(s) 937
Bse118I RCCGGY 2 cut(s) 663, 1005
Bse1I ACTGG 2 cut(s) 66, 240
Bse8I GATNNNNATC 1 cut(s) 1164
BseBI CCWGG 6 cut(s) 188, 375, 653, 673, 730, 795
BseDI CCNNGG 4 cut(s) 547, 884, 944, 962
BseGI GGATG 6 cut(s) 70, 94, 291, 502, 569, 1048
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 1 cut(s) 937
BseMII CTCAG 3 cut(s) 290, 450, 936
BseNI ACTGG 2 cut(s) 66, 240
BseSI GKGCMC 1 cut(s) 80
BseYI CCCAGC 1 cut(s) 935
Bsh1285I CGRYCG 1 cut(s) 956
BshFI GGCC 2 cut(s) 415, 961
BsiEI CGRYCG 1 cut(s) 956
BsiHKAI GWGCWC 1 cut(s) 747
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 3 cut(s) 512, 664, 1006
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 1 cut(s) 937
BsmAI GTCTC 1 cut(s) 1119
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 2 cut(s) 415, 961
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 3 cut(s) 80, 747, 952
Bsp143I GATC 4 cut(s) 146, 283, 953, 1165
Bsp1720I GCTNAGC 1 cut(s) 922
BspACI CCGC 1 cut(s) 845
BspANI GGCC 2 cut(s) 415, 961
BspCNI CTCAG 3 cut(s) 291, 451, 935
BspLI GGNNCC 2 cut(s) 553, 942
BspMI ACCTGC 1 cut(s) 977
BspOI GCTAGC 1 cut(s) 845
BspPI GGATC 1 cut(s) 1173
BsrFI RCCGGY 2 cut(s) 663, 1005
BsrI ACTGG 2 cut(s) 66, 240
BssAI RCCGGY 2 cut(s) 663, 1005
BssECI CCNNGG 4 cut(s) 547, 884, 944, 962
BssMI GATC 4 cut(s) 146, 283, 953, 1165
BssNI GRCGYC 1 cut(s) 1132
BssT1I CCWWGG 3 cut(s) 884, 944, 962
Bst2UI CCWGG 6 cut(s) 188, 375, 653, 673, 730, 795
Bst4CI ACNGT 5 cut(s) 194, 227, 260, 932, 981
BstACI GRCGYC 1 cut(s) 1132
BstC8I GCNNGC 4 cut(s) 128, 665, 669, 843
BstDEI CTNAG 3 cut(s) 299, 459, 922
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 6 cut(s) 70, 94, 291, 502, 569, 1048
BstHHI GCGC 1 cut(s) 663
BstKTI GATC 4 cut(s) 149, 286, 956, 1168
BstMAI GTCTC 1 cut(s) 1119
BstMBI GATC 4 cut(s) 146, 283, 953, 1165
BstMCI CGRYCG 1 cut(s) 956
BstMWI GCNNNNNNNGC 4 cut(s) 84, 534, 543, 932
BstNI CCWGG 6 cut(s) 188, 375, 653, 673, 730, 795
BstNSI RCATGY 2 cut(s) 671, 1090
BstSCI CCNGG 6 cut(s) 186, 373, 651, 671, 728, 793
BstSLI GKGCMC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 1028
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuRI GGCC 2 cut(s) 415, 961
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 6 cut(s) 70, 94, 291, 502, 569, 1048
BveI ACCTGC 1 cut(s) 977
Cac8I GCNNGC 4 cut(s) 128, 665, 669, 843
CaiI CAGNNNCTG 1 cut(s) 800
CfoI GCGC 1 cut(s) 663
Cfr10I RCCGGY 2 cut(s) 663, 1005
Cfr13I GGNCC 5 cut(s) 91, 190, 266, 881, 1155
CseI GACGC 1 cut(s) 1121
CviAII CATG 9 cut(s) 44, 88, 131, 485, 630, 668, 790, 1087, 1173
DdeI CTNAG 3 cut(s) 299, 459, 922
DpnI GATC 4 cut(s) 148, 285, 955, 1167
DpnII GATC 4 cut(s) 146, 283, 953, 1165
DraI TTTAAA 3 cut(s) 199, 408, 996
Eco130I CCWWGG 3 cut(s) 884, 944, 962
Eco147I AGGCCT 2 cut(s) 415, 961
Eco24I GRGCYC 1 cut(s) 952
Eco47I GGWCC 5 cut(s) 91, 190, 266, 881, 1155
Eco57I CTGAAG 2 cut(s) 623, 1022
Eco88I CYCGRG 1 cut(s) 621
EcoRII CCWGG 6 cut(s) 186, 373, 651, 671, 728, 793
EcoT14I CCWWGG 3 cut(s) 884, 944, 962
EcoT38I GRGCYC 1 cut(s) 952
ErhI CCWWGG 3 cut(s) 884, 944, 962
FaeI CATG 9 cut(s) 47, 91, 134, 488, 633, 671, 793, 1090, 1176
FaqI GGGAC 1 cut(s) 827
FatI CATG 9 cut(s) 43, 87, 130, 484, 629, 667, 789, 1086, 1172
FauI CCCGC 1 cut(s) 838
FokI GGATG 6 cut(s) 77, 81, 278, 509, 576, 1055
FriOI GRGCYC 1 cut(s) 952
FspBI CTAG 5 cut(s) 219, 270, 693, 717, 842
GlaI GCGC 1 cut(s) 662
GsaI CCCAGC 1 cut(s) 939
HaeIII GGCC 2 cut(s) 415, 961
HapII CCGG 3 cut(s) 512, 664, 1006
HgaI GACGC 1 cut(s) 1121
HhaI GCGC 1 cut(s) 663
Hin1I GRCGYC 1 cut(s) 1132
Hin1II CATG 9 cut(s) 47, 91, 134, 488, 633, 671, 793, 1090, 1176
Hin6I GCGC 1 cut(s) 661
HinP1I GCGC 1 cut(s) 661
HinfI GANTC 6 cut(s) 32, 308, 455, 461, 515, 626
HpaII CCGG 3 cut(s) 512, 664, 1006
HphI GGTGA 4 cut(s) 501, 674, 773, 1031
Hpy166II GTNNAC 2 cut(s) 682, 891
Hpy188I TCNGA 8 cut(s) 37, 283, 288, 300, 460, 703, 912, 1159
Hpy188III TCNNGA 6 cut(s) 401, 470, 623, 642, 717, 1040
Hpy8I GTNNAC 2 cut(s) 682, 891
Hpy99I CGWCG 2 cut(s) 981, 1137
HpyAV CCTTC 2 cut(s) 428, 1122
HpyCH4III ACNGT 5 cut(s) 194, 227, 260, 932, 981
HpyCH4V TGCA 3 cut(s) 87, 144, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 534, 543, 932
HpyF3I CTNAG 3 cut(s) 299, 459, 922
Hsp92I GRCGYC 1 cut(s) 1132
Hsp92II CATG 9 cut(s) 47, 91, 134, 488, 633, 671, 793, 1090, 1176
HspAI GCGC 1 cut(s) 661
KroI GCCGGC 1 cut(s) 663
KroNI GCCGGC 1 cut(s) 665
Kzo9I GATC 4 cut(s) 146, 283, 953, 1165
LmnI GCTCC 1 cut(s) 551
LweI GCATC 2 cut(s) 55, 571
MaeI CTAG 5 cut(s) 219, 270, 693, 717, 842
MaeIII GTNAC 3 cut(s) 167, 329, 516
MalI GATC 4 cut(s) 148, 285, 955, 1167
MboI GATC 4 cut(s) 146, 283, 953, 1165
MboII GAAGA 4 cut(s) 30, 590, 616, 1028
MfeI CAATTG 1 cut(s) 521
MhlI GDGCHC 3 cut(s) 80, 747, 952
MluCI AATT 7 cut(s) 181, 349, 367, 504, 521, 755, 1024
MlyI GAGTC 3 cut(s) 302, 470, 524
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 9 cut(s) 201, 306, 562, 709, 845, 951, 1060, 1146, 1156
MroNI GCCGGC 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 616
MseI TTAA 8 cut(s) 51, 198, 366, 407, 597, 995, 1098, 1142
MslI CAYNNNNRTG 3 cut(s) 86, 154, 1091
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 3 cut(s) 512, 664, 1006
MspR9I CCNGG 6 cut(s) 188, 375, 653, 673, 730, 795
MunI CAATTG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 6 cut(s) 188, 375, 653, 673, 730, 795
MwoI GCNNNNNNNGC 4 cut(s) 84, 534, 543, 932
NaeI GCCGGC 1 cut(s) 665
NdeII GATC 4 cut(s) 146, 283, 953, 1165
NgoMIV GCCGGC 1 cut(s) 663
NheI GCTAGC 1 cut(s) 841
NlaIII CATG 9 cut(s) 47, 91, 134, 488, 633, 671, 793, 1090, 1176
NlaIV GGNNCC 2 cut(s) 553, 942
NmuCI GTSAC 2 cut(s) 329, 516
NspI RCATGY 2 cut(s) 671, 1090
PaeI GCATGC 1 cut(s) 671
PaqCI CACCTGC 1 cut(s) 977
PceI AGGCCT 2 cut(s) 415, 961
PcsI WCGNNNNNNNCGW 2 cut(s) 730, 1056
PctI GAATGC 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 665
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 3 cut(s) 32, 455, 626
Ple19I CGATCG 1 cut(s) 956
PleI GAGTC 3 cut(s) 302, 469, 523
PpsI GAGTC 3 cut(s) 302, 469, 523
PshBI ATTAAT 1 cut(s) 1098
Psp6I CCWGG 6 cut(s) 186, 373, 651, 671, 728, 793
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 6 cut(s) 186, 373, 651, 671, 728, 793
PspN4I GGNNCC 2 cut(s) 553, 942
PspPI GGNCC 5 cut(s) 91, 190, 266, 881, 1155
PstNI CAGNNNCTG 1 cut(s) 800
PvuI CGATCG 1 cut(s) 956
PvuII CAGCTG 1 cut(s) 926
RseI CAYNNNNRTG 3 cut(s) 86, 154, 1091
SaqAI TTAA 8 cut(s) 51, 198, 366, 407, 597, 995, 1098, 1142
Sau3AI GATC 4 cut(s) 146, 283, 953, 1165
Sau96I GGNCC 5 cut(s) 91, 190, 266, 881, 1155
SchI GAGTC 3 cut(s) 302, 470, 524
ScrFI CCNGG 6 cut(s) 188, 375, 653, 673, 730, 795
SduI GDGCHC 3 cut(s) 80, 747, 952
SfaNI GCATC 2 cut(s) 55, 571
SinI GGWCC 5 cut(s) 91, 190, 266, 881, 1155
SmiI ATTTAAAT 1 cut(s) 408
SmiMI CAYNNNNRTG 3 cut(s) 86, 154, 1091
SmlI CTYRAG 2 cut(s) 740, 1150
SmoI CTYRAG 2 cut(s) 740, 1150
SphI GCATGC 1 cut(s) 671
Sse9I AATT 7 cut(s) 181, 349, 367, 504, 521, 755, 1024
SseBI AGGCCT 2 cut(s) 415, 961
SsiI CCGC 1 cut(s) 845
SspMI CTAG 5 cut(s) 219, 270, 693, 717, 842
StuI AGGCCT 2 cut(s) 415, 961
StyD4I CCNGG 6 cut(s) 186, 373, 651, 671, 728, 793
StyI CCWWGG 3 cut(s) 884, 944, 962
SwaI ATTTAAAT 1 cut(s) 408
TaaI ACNGT 5 cut(s) 194, 227, 260, 932, 981
TaqI TCGA 4 cut(s) 952, 956, 976, 1168
TasI AATT 7 cut(s) 181, 349, 367, 504, 521, 755, 1024
TfiI GAWTC 3 cut(s) 32, 455, 626
Tru1I TTAA 8 cut(s) 51, 198, 366, 407, 597, 995, 1098, 1142
Tru9I TTAA 8 cut(s) 51, 198, 366, 407, 597, 995, 1098, 1142
TseFI GTSAC 2 cut(s) 329, 516
Tsp45I GTSAC 2 cut(s) 329, 516
TspDTI ATGAA 5 cut(s) 32, 330, 339, 770, 1014
VpaK11BI GGWCC 5 cut(s) 91, 190, 266, 881, 1155
VspI ATTAAT 1 cut(s) 1098
XapI RAATTY 3 cut(s) 504, 755, 1024
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 2 cut(s) 671, 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 616
XspI CTAG 5 cut(s) 219, 270, 693, 717, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.