Rorug06G0172400

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002431
Physical Location & Seq
Reverse (-)
25480078 .. 25484681
4604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0172400.1

Sequence Viewer

Length: 1236 bp
ATGGATGAGAAGAATGGTCAAGTTGAAGAATCAAGCAAGGCGTGCAGCGACGGCTCCGTCTGTGGCTACGACTCACTTCACCACCTCCTCAGCGCAAATCTCAAACCCCATCTCTTCCAGGAAGTCAACCGTATTCTCTTAGGTCTGAATTGTGGCAAGGCACTAGAAACAATTTCTGTCCCGGAATCTGCTAAAGCGCTCTCTTCAGAACATGATTTTGACCTCCAGGCTTTCTGCTTTCATGCTGACAAAGAGTTGTTGAGGGAACCTCGAATAGTCAGGGTTGGTCTCATTCAAAACTCTATAGCTGTTCCAACTACTGCCCACTTTCTGGACCAGAAGAGGGCTATCTTTGGGAAATTAAAGCCAATAATTGATGCTGCAGCTGTTTCAGGAGTAAACATATTATGCTTGCAGGAAGCATGGACAATGCCCTTTGCCTTTTGTACACGGGAGAAGAGGTGGTGTGAATTTGCAGAGCCTGTAGATGGGGAATCAACACAATTTCTCCAGGATCTTGCACGGAAACATAACATGGTCATCATAAGTCCTATTCTTGAGAGGGATGTCAATCATGGGGAGACTCTGTGGAACACTGCTGTCATAATTGGAAATCATGGCAACATAATTGGCAAGCATCGAAAGAACCATATCCCAAGAGTTGGGGACTTCAATGAGAGTACCTATTATATGGAAGGAAATACCGGGCATCCAGTGTTTGAGACTGCTTATGGAAAAATTGCGGTAAATATATGTTACGGAAGGCACCATCCTTTGAATTGGTTGGCATTTGGCTTGAATGGTGCAGAGATTGTCTTCAACCCTTCTGCCACTGTTGGTGAACTCAGTGAACCAATGTGGCCCATTGAGGCTCGTAATGCTGCAATTGCAAATAGTTACTTTGTTGGATCAATCAACCGTGTAGGAACAGAGGTTTTCCCCAATCCATTTACTTCTGGTGATGGCAAGCCGCAACATGCAGACTTTGGTCATTTCTATGGGTCAAGTTATTTTTCTTCCCCAGATGCCTCATGCACCCCTTCTCTATCACGCCATCGTGATGGGTTGTTGATATCAGACATGGACCTCAACCTCTGTAGACAGATAAAAGACAAGTGGGGATTCCGAATGACTGCTCGTTACGAGTTGTATGGTGATCTTCTTGATCGTTATTTGAAGCCAGATTTTGAGCCCCAAGTCATTTCTGATCCCATGTTGCATAAGAAGACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

411

Amino Acids

45.93

Weight (kDa)

6.05

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 96 - 370 9.6e-57 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccB1I GGYRCC 1 cut(s) 765
AccB7I CCANNNNNTGG 2 cut(s) 331, 662
AccI GTMKAC 1 cut(s) 1099
AciI CCGC 2 cut(s) 743, 971
AclWI GGATC 3 cut(s) 522, 916, 1202
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 2 cut(s) 448, 682
AfeI AGCGCT 1 cut(s) 198
AfiI CCNNNNNNNGG 4 cut(s) 331, 343, 488, 662
AgsI TTSAA 7 cut(s) 26, 296, 673, 778, 799, 820, 1177
AjnI CCWGG 3 cut(s) 117, 225, 510
AluBI AGCT 2 cut(s) 308, 386
AluI AGCT 2 cut(s) 308, 386
Alw26I GTCTC 3 cut(s) 293, 575, 716
AlwI GGATC 3 cut(s) 522, 916, 1202
AlwNI CAGNNNCTG 1 cut(s) 482
Aor51HI AGCGCT 1 cut(s) 198
AoxI GGCC 1 cut(s) 860
ApeKI GCWGC 4 cut(s) 45, 380, 383, 881
ApoI RAATTY 1 cut(s) 470
AspLEI GCGC 2 cut(s) 95, 199
AspS9I GGNCC 3 cut(s) 334, 861, 1084
AsuC2I CCSGG 2 cut(s) 182, 706
AsuHPI GGTGA 4 cut(s) 71, 851, 971, 1166
AvaII GGWCC 2 cut(s) 334, 1084
BanI GGYRCC 1 cut(s) 765
BanII GRGCYC 1 cut(s) 1194
BbsI GAAGAC 2 cut(s) 808, 1232
BbvCI CCTCAGC 1 cut(s) 89
BbvI GCAGC 4 cut(s) 57, 367, 395, 868
BccI CCATC 6 cut(s) 117, 482, 777, 956, 1055, 1062
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 3 cut(s) 119, 227, 512
BcnI CCSGG 2 cut(s) 182, 706
BcoDI GTCTC 3 cut(s) 293, 575, 716
BfaI CTAG 1 cut(s) 164
BfmI CTRYAG 4 cut(s) 303, 381, 483, 1096
BfoI RGCGCY 1 cut(s) 200
BisI GCNGC 5 cut(s) 46, 381, 384, 882, 971
BlsI GCNGC 5 cut(s) 47, 382, 385, 883, 972
Bme1390I CCNGG 5 cut(s) 119, 182, 227, 512, 706
Bme18I GGWCC 2 cut(s) 334, 1084
BmgT120I GGNCC 3 cut(s) 334, 861, 1084
BmiI GGNNCC 3 cut(s) 55, 267, 767
BmrFI CCNGG 5 cut(s) 119, 182, 227, 512, 706
BmsI GCATC 4 cut(s) 367, 646, 718, 1015
BoxI GACNNNNGTC 1 cut(s) 987
BpiI GAAGAC 2 cut(s) 808, 1232
BplI GAGNNNNNCTC 2 cut(s) 253, 285
BpmI CTGGAG 2 cut(s) 209, 494
Bpu10I CCTNAGC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 578
BpuMI CCSGG 2 cut(s) 182, 706
BsaBI GATNNNNATC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 293
BsaXI ACNNNNNCTCC 6 cut(s) 446, 476, 492, 522, 572, 602
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 343, 488, 662
Bse1I ACTGG 1 cut(s) 713
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 119, 227, 512
BseGI GGATG 4 cut(s) 10, 571, 709, 769
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 4 cut(s) 331, 343, 488, 662
BseMII CTCAG 2 cut(s) 103, 859
BseNI ACTGG 1 cut(s) 713
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 4 cut(s) 57, 367, 395, 868
BsgI GTGCAG 2 cut(s) 64, 825
BshFI GGCC 1 cut(s) 862
BshNI GGYRCC 1 cut(s) 765
BsiSI CCGG 2 cut(s) 182, 705
BslFI GGGAC 2 cut(s) 164, 680
BslI CCNNNNNNNGG 4 cut(s) 331, 343, 488, 662
BsmAI GTCTC 3 cut(s) 293, 575, 716
BsmFI GGGAC 2 cut(s) 164, 680
BsnI GGCC 1 cut(s) 862
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 1194
Bsp1407I TGTACA 1 cut(s) 446
Bsp143I GATC 5 cut(s) 514, 908, 1156, 1165, 1207
BspACI CCGC 2 cut(s) 743, 971
BspANI GGCC 1 cut(s) 862
BspCNI CTCAG 2 cut(s) 102, 858
BspLI GGNNCC 3 cut(s) 55, 267, 767
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 3 cut(s) 522, 916, 1202
BspT107I GGYRCC 1 cut(s) 765
BspTNI GGTCTC 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 446
BsrI ACTGG 1 cut(s) 713
BssMI GATC 5 cut(s) 514, 908, 1156, 1165, 1207
Bst2UI CCWGG 3 cut(s) 119, 227, 512
Bst4CI ACNGT 3 cut(s) 131, 835, 920
Bst6I CTCTTC 4 cut(s) 119, 208, 335, 452
BstAPI GCANNNNNTGC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 446
BstC8I GCNNGC 4 cut(s) 43, 413, 635, 968
BstDEI CTNAG 3 cut(s) 89, 139, 845
BstF5I GGATG 4 cut(s) 10, 571, 709, 769
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 2 cut(s) 95, 199
BstKTI GATC 5 cut(s) 517, 911, 1159, 1168, 1210
BstMAI GTCTC 3 cut(s) 293, 575, 716
BstMBI GATC 5 cut(s) 514, 908, 1156, 1165, 1207
BstMWI GCNNNNNNNGC 4 cut(s) 42, 51, 878, 887
BstNI CCWGG 3 cut(s) 119, 227, 512
BstNSI RCATGY 1 cut(s) 980
BstPAI GACNNNNGTC 1 cut(s) 987
BstSCI CCNGG 5 cut(s) 117, 180, 225, 510, 704
BstSFI CTRYAG 4 cut(s) 303, 381, 483, 1096
BstV1I GCAGC 4 cut(s) 57, 367, 395, 868
BstV2I GAAGAC 2 cut(s) 808, 1232
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 1 cut(s) 1061
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 1 cut(s) 862
BtsCI GGATG 4 cut(s) 10, 571, 709, 769
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 4 cut(s) 594, 720, 831, 853
Cac8I GCNNGC 4 cut(s) 43, 413, 635, 968
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 2 cut(s) 95, 199
Cfr13I GGNCC 3 cut(s) 334, 861, 1084
Csp6I GTAC 2 cut(s) 447, 681
CviQI GTAC 2 cut(s) 447, 681
DdeI CTNAG 3 cut(s) 89, 139, 845
DpnI GATC 5 cut(s) 516, 910, 1158, 1167, 1209
DpnII GATC 5 cut(s) 514, 908, 1156, 1165, 1207
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
Eam1104I CTCTTC 4 cut(s) 119, 208, 335, 452
EarI CTCTTC 4 cut(s) 119, 208, 335, 452
Eco24I GRGCYC 1 cut(s) 1194
Eco31I GGTCTC 1 cut(s) 293
Eco32I GATATC 1 cut(s) 1074
Eco47I GGWCC 2 cut(s) 334, 1084
Eco47III AGCGCT 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 189
EcoRII CCWGG 3 cut(s) 117, 225, 510
EcoRV GATATC 1 cut(s) 1074
EcoT38I GRGCYC 1 cut(s) 1194
FaqI GGGAC 2 cut(s) 164, 680
FblI GTMKAC 1 cut(s) 1099
Fnu4HI GCNGC 5 cut(s) 46, 381, 384, 882, 971
FokI GGATG 4 cut(s) 17, 578, 696, 756
FriOI GRGCYC 1 cut(s) 1194
Fsp4HI GCNGC 5 cut(s) 46, 381, 384, 882, 971
FspBI CTAG 1 cut(s) 164
GlaI GCGC 2 cut(s) 94, 198
GluI GCNGC 5 cut(s) 46, 381, 384, 882, 971
GsuI CTGGAG 2 cut(s) 209, 494
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 1 cut(s) 862
HapII CCGG 2 cut(s) 182, 705
HhaI GCGC 2 cut(s) 95, 199
Hin6I GCGC 2 cut(s) 93, 197
HinP1I GCGC 2 cut(s) 93, 197
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 6 cut(s) 29, 71, 185, 494, 583, 1122
HpaII CCGG 2 cut(s) 182, 705
HphI GGTGA 4 cut(s) 71, 851, 971, 1166
Hpy166II GTNNAC 6 cut(s) 127, 400, 449, 842, 851, 1100
Hpy188I TCNGA 5 cut(s) 147, 208, 1078, 1127, 1207
Hpy188III TCNNGA 5 cut(s) 332, 393, 557, 1058, 1163
Hpy8I GTNNAC 6 cut(s) 127, 400, 449, 842, 851, 1100
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 4 cut(s) 689, 756, 834, 1050
HpyCH4III ACNGT 3 cut(s) 131, 835, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 51, 878, 887
HpyF3I CTNAG 3 cut(s) 89, 139, 845
HspAI GCGC 2 cut(s) 93, 197
Kzo9I GATC 5 cut(s) 514, 908, 1156, 1165, 1207
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 4 cut(s) 57, 367, 395, 868
LweI GCATC 4 cut(s) 367, 646, 718, 1015
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 3 cut(s) 755, 896, 1139
MalI GATC 5 cut(s) 516, 910, 1158, 1167, 1209
MboI GATC 5 cut(s) 514, 908, 1156, 1165, 1207
MboII GAAGA 9 cut(s) 22, 38, 106, 195, 352, 469, 808, 1008, 1151
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 1194
MlyI GAGTC 2 cut(s) 65, 577
MmeI TCCRAC 2 cut(s) 338, 886
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 2 cut(s) 996, 1059
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 2 cut(s) 182, 705
MspR9I CCNGG 5 cut(s) 119, 182, 227, 512, 706
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 3 cut(s) 119, 227, 512
MwoI GCNNNNNNNGC 4 cut(s) 42, 51, 878, 887
NciI CCSGG 2 cut(s) 182, 706
NdeII GATC 5 cut(s) 514, 908, 1156, 1165, 1207
NlaIV GGNNCC 3 cut(s) 55, 267, 767
NspI RCATGY 1 cut(s) 980
PfeI GAWTC 4 cut(s) 29, 185, 494, 1122
PflMI CCANNNNNTGG 2 cut(s) 331, 662
PfoI TCCNGGA 3 cut(s) 117, 180, 510
PkrI GCNGC 5 cut(s) 47, 382, 385, 883, 972
PleI GAGTC 2 cut(s) 65, 577
PpsI GAGTC 2 cut(s) 65, 577
PshAI GACNNNNGTC 1 cut(s) 987
Psp6I CCWGG 3 cut(s) 117, 225, 510
PspGI CCWGG 3 cut(s) 117, 225, 510
PspN4I GGNNCC 3 cut(s) 55, 267, 767
PspPI GGNCC 3 cut(s) 334, 861, 1084
PstI CTGCAG 1 cut(s) 385
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 1 cut(s) 514
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 448, 682
RsaNI GTAC 2 cut(s) 447, 681
RseI CAYNNNNRTG 2 cut(s) 996, 1059
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 5 cut(s) 46, 381, 384, 882, 971
Sau3AI GATC 5 cut(s) 514, 908, 1156, 1165, 1207
Sau96I GGNCC 3 cut(s) 334, 861, 1084
SchI GAGTC 2 cut(s) 65, 577
ScrFI CCNGG 5 cut(s) 119, 182, 227, 512, 706
SduI GDGCHC 1 cut(s) 1194
SfaNI GCATC 4 cut(s) 367, 646, 718, 1015
SfcI CTRYAG 4 cut(s) 303, 381, 483, 1096
SinI GGWCC 2 cut(s) 334, 1084
SmiMI CAYNNNNRTG 2 cut(s) 996, 1059
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SsiI CCGC 2 cut(s) 743, 971
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 5 cut(s) 117, 180, 225, 510, 704
TaaI ACNGT 3 cut(s) 131, 835, 920
TaqI TCGA 2 cut(s) 271, 640
TatI WGTACW 1 cut(s) 446
TauI GCSGC 1 cut(s) 973
TfiI GAWTC 4 cut(s) 29, 185, 494, 1122
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TscAI CASTG 4 cut(s) 601, 720, 838, 853
TseI GCWGC 4 cut(s) 45, 380, 383, 881
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 3 cut(s) 46, 538, 774
TspRI CASTG 4 cut(s) 601, 720, 838, 853
Van91I CCANNNNNTGG 2 cut(s) 331, 662
VpaK11BI GGWCC 2 cut(s) 334, 1084
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 980
XmiI GTMKAC 1 cut(s) 1099
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.