Rroxscaffold_7G00183590

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002431
Physical Location & Seq
Forward (+)
22683029 .. 22686809
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183590.1

Sequence Viewer

Length: 1311 bp
ATGTATACATTCATTATACATGCTTCCATTGTTTTATGTGATATGAAAAATAAGTTGTTGTTTTCTGCAATCGCCCTCCCTATTGATTGGTACTTGTGTCCTTGTCCTAATAAGTTCCACAGTAAATTAAACAGTTTATGCAAGATGGCTGGTGTGAAACGAAGAATAGATACTGGTTCAGATATTCGTGCTCTTTACAAAGAACTGGATGCTGTTTCATGCCCTATCTGCATGGACCATCCACATAATGCAGTTCTCCTCCTTTGCAGCTCGCATGACAAGGGTTGCAGATCTTACATCTGTGATACGAGTTACAGGCATTCAAATTGCCTGGACCGTTTTAAAAAGTTGAGGGAAAATAATACAAACAGTCCAACTTTAGTCAGTTCTTTACCTACAAACCATCATGGCTCTCATAATACTTCTGATATGGCATCGGGAACAGATTTGAATGAAGCTAATGAAAGTCCTAACTTAATTGAAATTGAAAGCAATGCTGTAATATCTGCCCATTCACCTGGACAGCCACAGGAACGTGTTATTCAAGATCTAAATATGCCACTTCTGCCAGAAGAACTTTTGGGAGTCGCTGATTCCGAGTCAGTTCAGGAAAGGGTTGAGCATAGTGAGTTGGATGTGGAAAATTCATCAGAGTCAAATTTGAGCTTGAAATGTCCTTTGTGCCGGGGAGCCATCCTTGGTTGGGAGGTTGTGGAAGATTGCAGAAAGTATCTTAATCTGAAGAAGCGAAGTTGTTCTCGTGAAGGATGCTCCTTTTCTGGTAACTACCAGGAGTTGCGTCGGCATGCTAGGAGAGTTCATCCCGCTACTCGACCGTCAGACATTGACCCATCTAGAGAGCGAGCCTGGAGACACCTTGAGCACCAGAGAGAATTTGGTGATGTAGTTAGTGCTATTCATTCAGCCATGCCAGGAGCTGTTGTGGTTGGGGATTATGTTATTGAAAATGGAGATAGGCTAGGGGGTGGAGGGGAAATTGGTACTGGTGAAGCTAATGGCCCTTGGTGGACTACCATGTTTTTGTTTCAGATGATTGGTTCAGTTGATCGTGCTGGGGAACCAAGGGCAAGGGCAAGGGCTTGGACTAGACATCGGCGGTCAGCGGGAGCTTTGTCCGAACGCCGGTTACTTTGGGGTGAGAATCTGTTGGGTCTTCAGGATGATGATGAAGATGATGAGGATGATGATGAGGAGGACAATATTCTCATGCTGAATGACAGAGATGTATCTCCAATTCCCAGAAGGCGCCGGCGTTTGACAAGGTCTAGGTCAGATGAAGATCGACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

436

Amino Acids

49.15

Weight (kDa)

5.81

Isoelectric Point (pI)

61.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 71 - 304 2e-70 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1266
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 825, 1117, 1124
AcsI RAATTY 3 cut(s) 643, 658, 893
AcuI CTGAAG 2 cut(s) 761, 1160
AcyI GRCGYC 1 cut(s) 1267
AfaI GTAC 2 cut(s) 92, 1003
AfiI CCNNNNNNNGG 2 cut(s) 703, 1143
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 7 cut(s) 324, 451, 482, 488, 545, 670, 965
AjnI CCWGG 5 cut(s) 330, 517, 789, 866, 931
AluBI AGCT 6 cut(s) 270, 458, 666, 938, 1013, 1130
AluI AGCT 6 cut(s) 270, 458, 666, 938, 1013, 1130
Alw21I GWGCWC 2 cut(s) 193, 885
Alw26I GTCTC 1 cut(s) 865
AlwNI CAGNNNCTG 1 cut(s) 938
AoxI GGCC 1 cut(s) 1018
ApeKI GCWGC 1 cut(s) 267
ApoI RAATTY 3 cut(s) 643, 658, 893
Asp700I GAANNNNTTC 1 cut(s) 754
AspLEI GCGC 1 cut(s) 1269
AspS9I GGNCC 3 cut(s) 235, 334, 1019
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 4 cut(s) 507, 911, 1019, 1169
AvaII GGWCC 2 cut(s) 235, 334
BaeI ACNNNNGTAYC 2 cut(s) 82, 115
BanI GGYRCC 1 cut(s) 1266
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
BauI CACGAG 1 cut(s) 759
BbsI GAAGAC 1 cut(s) 1166
Bbv12I GWGCWC 2 cut(s) 193, 885
BbvI GCAGC 1 cut(s) 279
BccI CCATC 5 cut(s) 139, 246, 411, 701, 859
BciT130I CCWGG 5 cut(s) 332, 519, 791, 868, 933
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 1 cut(s) 865
BfaI CTAG 5 cut(s) 810, 855, 980, 1107, 1287
BfoI RGCGCY 1 cut(s) 1270
BglII AGATCT 2 cut(s) 290, 547
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
Bme1390I CCNGG 6 cut(s) 332, 519, 686, 791, 868, 933
Bme18I GGWCC 2 cut(s) 235, 334
BmgT120I GGNCC 3 cut(s) 235, 334, 1019
BmiI GGNNCC 3 cut(s) 691, 1080, 1268
BmrFI CCNGG 6 cut(s) 332, 519, 686, 791, 868, 933
BmsI GCATC 3 cut(s) 199, 443, 758
BpiI GAAGAC 1 cut(s) 1166
BpmI CTGGAG 1 cut(s) 889
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 1299
BsaHI GRCGYC 1 cut(s) 1267
BsaJI CCNNGG 4 cut(s) 685, 697, 1022, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 927, 957
Bsc4I CCNNNNNNNGG 2 cut(s) 703, 1143
Bse118I RCCGGY 2 cut(s) 1143, 1269
Bse1I ACTGG 3 cut(s) 178, 210, 1009
Bse3DI GCAATG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 1299
BseBI CCWGG 5 cut(s) 332, 519, 791, 868, 933
BseDI CCNNGG 4 cut(s) 685, 697, 1022, 1082
BseGI GGATG 8 cut(s) 214, 238, 640, 693, 773, 820, 1186, 1207
BseJI GATNNNNATC 1 cut(s) 1299
BseLI CCNNNNNNNGG 2 cut(s) 703, 1143
BseMI GCAATG 1 cut(s) 499
BseNI ACTGG 3 cut(s) 178, 210, 1009
BseRI GAGGAG 2 cut(s) 248, 1226
BseXI GCAGC 1 cut(s) 279
BseYI CCCAGC 1 cut(s) 1073
Bsh1285I CGRYCG 1 cut(s) 836
BshFI GGCC 1 cut(s) 1020
BshNI GGYRCC 1 cut(s) 1266
BsiEI CGRYCG 1 cut(s) 836
BsiHKAI GWGCWC 2 cut(s) 193, 885
BsiSI CCGG 3 cut(s) 685, 1144, 1270
BslI CCNNNNNNNGG 2 cut(s) 703, 1143
BsmAI GTCTC 1 cut(s) 865
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 193, 885
Bsp143I GATC 4 cut(s) 290, 547, 1066, 1300
BspACI CCGC 3 cut(s) 825, 1117, 1124
BspANI GGCC 1 cut(s) 1020
BspLI GGNNCC 3 cut(s) 691, 1080, 1268
BspT107I GGYRCC 1 cut(s) 1266
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 2 cut(s) 1143, 1269
BsrI ACTGG 3 cut(s) 178, 210, 1009
BssAI RCCGGY 2 cut(s) 1143, 1269
BssECI CCNNGG 4 cut(s) 685, 697, 1022, 1082
BssMI GATC 4 cut(s) 290, 547, 1066, 1300
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 1267
BssSI CACGAG 1 cut(s) 759
BssT1I CCWWGG 3 cut(s) 697, 1022, 1082
Bst1107I GTATAC 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 759
Bst2UI CCWGG 5 cut(s) 332, 519, 791, 868, 933
Bst4CI ACNGT 5 cut(s) 122, 134, 338, 371, 837
BstACI GRCGYC 1 cut(s) 1267
BstC8I GCNNGC 4 cut(s) 272, 807, 864, 1271
BstF5I GGATG 8 cut(s) 214, 238, 640, 693, 773, 820, 1186, 1207
BstH2I RGCGCY 1 cut(s) 1270
BstHHI GCGC 1 cut(s) 1269
BstKTI GATC 4 cut(s) 293, 550, 1069, 1303
BstMAI GTCTC 1 cut(s) 865
BstMBI GATC 4 cut(s) 290, 547, 1066, 1300
BstMCI CGRYCG 1 cut(s) 836
BstMWI GCNNNNNNNGC 2 cut(s) 228, 565
BstNI CCWGG 5 cut(s) 332, 519, 791, 868, 933
BstNSI RCATGY 2 cut(s) 23, 809
BstSCI CCNGG 6 cut(s) 330, 517, 684, 789, 866, 931
BstV1I GCAGC 1 cut(s) 279
BstV2I GAAGAC 1 cut(s) 1166
BstX2I RGATCY 2 cut(s) 290, 547
BstXI CCANNNNNNTGG 1 cut(s) 518
BstYI RGATCY 2 cut(s) 290, 547
BstZ17I GTATAC 1 cut(s) 6
BsuRI GGCC 1 cut(s) 1020
BtsCI GGATG 8 cut(s) 214, 238, 640, 693, 773, 820, 1186, 1207
Cac8I GCNNGC 4 cut(s) 272, 807, 864, 1271
CaiI CAGNNNCTG 1 cut(s) 938
CfoI GCGC 1 cut(s) 1269
Cfr10I RCCGGY 2 cut(s) 1143, 1269
Cfr13I GGNCC 3 cut(s) 235, 334, 1019
CseI GACGC 1 cut(s) 788
Csp6I GTAC 2 cut(s) 91, 1002
CviQI GTAC 2 cut(s) 91, 1002
DinI GGCGCC 1 cut(s) 1268
DpnI GATC 4 cut(s) 292, 549, 1068, 1302
DpnII GATC 4 cut(s) 290, 547, 1066, 1300
DraI TTTAAA 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 697, 1022, 1082
Eco47I GGWCC 2 cut(s) 235, 334
Eco57I CTGAAG 2 cut(s) 761, 1160
EcoRII CCWGG 5 cut(s) 330, 517, 789, 866, 931
EcoT14I CCWWGG 3 cut(s) 697, 1022, 1082
EgeI GGCGCC 1 cut(s) 1268
EheI GGCGCC 1 cut(s) 1268
ErhI CCWWGG 3 cut(s) 697, 1022, 1082
FauI CCCGC 2 cut(s) 832, 1117
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 8 cut(s) 221, 225, 647, 680, 780, 807, 1193, 1214
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 5 cut(s) 810, 855, 980, 1107, 1287
GlaI GCGC 1 cut(s) 1268
GluI GCNGC 1 cut(s) 268
GsaI CCCAGC 1 cut(s) 1077
GsuI CTGGAG 1 cut(s) 889
HaeII RGCGCY 1 cut(s) 1270
HaeIII GGCC 1 cut(s) 1020
HapII CCGG 3 cut(s) 685, 1144, 1270
HgaI GACGC 1 cut(s) 788
HhaI GCGC 1 cut(s) 1269
Hin1I GRCGYC 1 cut(s) 1267
Hin6I GCGC 1 cut(s) 1267
HinP1I GCGC 1 cut(s) 1267
HinfI GANTC 5 cut(s) 585, 593, 599, 653, 1162
HpaII CCGG 3 cut(s) 685, 1144, 1270
HphI GGTGA 4 cut(s) 507, 911, 1019, 1169
Hpy166II GTNNAC 2 cut(s) 6, 1029
Hpy188I TCNGA 9 cut(s) 181, 427, 598, 652, 741, 841, 1050, 1138, 1294
Hpy188III TCNNGA 6 cut(s) 438, 545, 608, 761, 855, 1178
Hpy8I GTNNAC 2 cut(s) 6, 1029
Hpy99I CGWCG 1 cut(s) 804
HpyAV CCTTC 2 cut(s) 758, 1257
HpyCH4III ACNGT 5 cut(s) 122, 134, 338, 371, 837
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 7 cut(s) 68, 141, 231, 251, 267, 288, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 565
HpySE526I ACGT 1 cut(s) 535
Hsp92I GRCGYC 1 cut(s) 1267
HspAI GCGC 1 cut(s) 1267
KasI GGCGCC 1 cut(s) 1266
KroI GCCGGC 1 cut(s) 1269
KroNI GCCGGC 1 cut(s) 1271
Kzo9I GATC 4 cut(s) 290, 547, 1066, 1300
LmnI GCTCC 4 cut(s) 689, 776, 935, 1127
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 3 cut(s) 199, 443, 758
MaeI CTAG 5 cut(s) 810, 855, 980, 1107, 1287
MaeII ACGT 1 cut(s) 535
MaeIII GTNAC 3 cut(s) 311, 782, 1146
MalI GATC 4 cut(s) 292, 549, 1068, 1302
MboI GATC 4 cut(s) 290, 547, 1066, 1300
MboII GAAGA 7 cut(s) 174, 584, 728, 754, 1166, 1202, 1310
MflI RGATCY 2 cut(s) 290, 547
MhlI GDGCHC 2 cut(s) 193, 885
MluCI AATT 9 cut(s) 125, 325, 477, 483, 643, 658, 893, 996, 1254
Mly113I GGCGCC 1 cut(s) 1267
MlyI GAGTC 3 cut(s) 594, 608, 662
MmeI TCCRAC 2 cut(s) 398, 612
MnlI CCTC 8 cut(s) 86, 269, 345, 700, 983, 1192, 1204, 1207
MreI CGCCGGCG 1 cut(s) 1269
MroNI GCCGGC 1 cut(s) 1269
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 4 cut(s) 128, 342, 476, 735
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 3 cut(s) 685, 1144, 1270
MspR9I CCNGG 6 cut(s) 332, 519, 686, 791, 868, 933
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 5 cut(s) 332, 519, 791, 868, 933
MwoI GCNNNNNNNGC 2 cut(s) 228, 565
NaeI GCCGGC 1 cut(s) 1271
NarI GGCGCC 1 cut(s) 1267
NciI CCSGG 1 cut(s) 686
NdeII GATC 4 cut(s) 290, 547, 1066, 1300
NgoMIV GCCGGC 1 cut(s) 1269
NlaIV GGNNCC 3 cut(s) 691, 1080, 1268
NspI RCATGY 2 cut(s) 23, 809
PaeI GCATGC 1 cut(s) 809
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PctI GAATGC 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 1271
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 2 cut(s) 593, 1162
PflFI GACNNNGTC 1 cut(s) 1282
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 3 cut(s) 593, 607, 661
PluTI GGCGCC 1 cut(s) 1270
PpsI GAGTC 3 cut(s) 593, 607, 661
Psp6I CCWGG 5 cut(s) 330, 517, 789, 866, 931
PspFI CCCAGC 1 cut(s) 1073
PspGI CCWGG 5 cut(s) 330, 517, 789, 866, 931
PspN4I GGNNCC 3 cut(s) 691, 1080, 1268
PspPI GGNCC 3 cut(s) 235, 334, 1019
PsrI GAACNNNNNNTAC 2 cut(s) 1131, 1163
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 2 cut(s) 290, 547
PsyI GACNNNGTC 1 cut(s) 1282
RsaI GTAC 2 cut(s) 92, 1003
RsaNI GTAC 2 cut(s) 91, 1002
SaqAI TTAA 4 cut(s) 128, 342, 476, 735
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 4 cut(s) 290, 547, 1066, 1300
Sau96I GGNCC 3 cut(s) 235, 334, 1019
SchI GAGTC 3 cut(s) 594, 608, 662
ScrFI CCNGG 6 cut(s) 332, 519, 686, 791, 868, 933
SduI GDGCHC 2 cut(s) 193, 885
SfaNI GCATC 3 cut(s) 199, 443, 758
SfoI GGCGCC 1 cut(s) 1268
SgrAI CRCCGGYG 1 cut(s) 1269
SinI GGWCC 2 cut(s) 235, 334
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 1 cut(s) 809
Sse9I AATT 9 cut(s) 125, 325, 477, 483, 643, 658, 893, 996, 1254
SsiI CCGC 3 cut(s) 825, 1117, 1124
SspDI GGCGCC 1 cut(s) 1266
SspI AATATT 1 cut(s) 1222
SspMI CTAG 5 cut(s) 810, 855, 980, 1107, 1287
StyD4I CCNGG 6 cut(s) 330, 517, 684, 789, 866, 931
StyI CCWWGG 3 cut(s) 697, 1022, 1082
TaaI ACNGT 5 cut(s) 122, 134, 338, 371, 837
TaiI ACGT 1 cut(s) 538
TaqI TCGA 2 cut(s) 832, 1303
TasI AATT 9 cut(s) 125, 325, 477, 483, 643, 658, 893, 996, 1254
TfiI GAWTC 2 cut(s) 593, 1162
Tru1I TTAA 4 cut(s) 128, 342, 476, 735
Tru9I TTAA 4 cut(s) 128, 342, 476, 735
TseI GCWGC 1 cut(s) 267
TspDTI ATGAA 9 cut(s) 59, 207, 468, 477, 636, 809, 908, 1203, 1311
Tth111I GACNNNGTC 1 cut(s) 1282
VpaK11BI GGWCC 2 cut(s) 235, 334
XapI RAATTY 3 cut(s) 643, 658, 893
XbaI TCTAGA 1 cut(s) 854
XceI RCATGY 2 cut(s) 23, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 5 cut(s) 810, 855, 980, 1107, 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.