MD05G1178600.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002431
Physical Location & Seq
Reverse (-)
30519282 .. 30523246
3965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1178600.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGGCTGGTGTGAAACGAAGATTAGATACTGATTCAGATATTCGTGCTCTTAATAAAGAACTGGATGCCGTTTCATGCCCTATCTGCATGGACCATCCGCATAATGCCGTTCTCCTGCTTTGCAGCTCACATGATAAGGGTTGCAGATCTTATATATGTGATACAAGTTACAGGCATTCAAATTGTTTGGACCGTTTTAAAAAGATGAGAGAAAATACTATGAACAGTCCAACTCTACCCAGTCCTTTCCCTATAAACCGTTACGGTCCTCGTATTTCTGATCTGAACATCCCTCTGAGAACAGACTCAAATGAAGCTAATGAAAGTGACAACCTGATTGAAAGCAATTCTGTATTATCTGTTAATTTACCTGGACCTCCACAGCAACATGTTATTCAAGATTTAAATAGGCCTTTTGAAGCACAACCAGAAGGTGTTATGGAAGTGGTTGATTCTGAGTCATTTCAGGAAAGGGATGAACATGATGATTTGGATGGGGAAAATTCACCGGAGTCACAATTGAGCTTGAAATGCCCTATGTGCCGAGGAGCCATTCTTGGATGGGAGGTTGTGGAAGATACCAGAAAATATCTTAACCTGAAGAAGCGAAGTTGCTCTCGGGAATCATGCTCATTTTCTGGTAACTACCAGGAGTTGCGCCGGCATGCCAGGAGAGTTCACCCAACCACCAGACCCTCTGATATTGACCCATCTAGAGAACGAGCCTGGCGACACCTTGAGCACCAGAGAGAATTTGGTGATGTTGTGAGTGCTATTCATTCAGCCATGCCTGGTGCTGTTGTTGTTGGGGACTATGTTATTGAAAATGGAGATAGGCTACCAGGTGGAGGGGAAAGTGGTGCAGGTGAAGCTAATGGGCCTTGGTGGACTACCCTGTTTTTGTTTCAGATGATTGGCTCAGCTGACCGTGCTGGAGAACCAAGGGCTCGATCAAGGGTTTGGACAAGACATAGGCGGTCAGCAGGTGCTTTAAATGAACGCCGGTTTCTTTGGGGTGAGAATTTGTTGGGACTTCAAGATGATGATGATGATGATGATGACAATGATGAGGACGATGATGATGACAACATGCCCATGACTAATCACAGAGATTTGTCTCCAATCCCCAGAAGGCGTCGACGTTTGACACGCTCAAGGTCCGATGAGGATCGACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

393

Amino Acids

44.61

Weight (kDa)

5.32

Isoelectric Point (pI)

64.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 23 - 257 1.3e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 854, 974
Acc36I ACCTGC 2 cut(s) 854, 974
AccI GTMKAC 1 cut(s) 1138
AciI CCGC 2 cut(s) 98, 976
AclWI GGATC 1 cut(s) 1176
AcsI RAATTY 3 cut(s) 502, 752, 1021
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 1135
AfiI CCNNNNNNNGG 1 cut(s) 848
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 7 cut(s) 180, 341, 398, 419, 529, 824, 1037
AjnI CCWGG 6 cut(s) 370, 648, 668, 725, 790, 841
AluBI AGCT 5 cut(s) 126, 317, 525, 872, 923
AluI AGCT 5 cut(s) 126, 317, 525, 872, 923
Alw21I GWGCWC 2 cut(s) 49, 744
Alw26I GTCTC 1 cut(s) 1122
AlwI GGATC 1 cut(s) 1176
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 2 cut(s) 410, 878
ApeKI GCWGC 1 cut(s) 123
ApoI RAATTY 3 cut(s) 502, 752, 1021
AspLEI GCGC 1 cut(s) 660
AspS9I GGNCC 6 cut(s) 91, 190, 266, 374, 878, 1158
AsuHPI GGTGA 5 cut(s) 498, 671, 770, 878, 1028
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 5 cut(s) 91, 190, 266, 374, 1158
BanII GRGCYC 1 cut(s) 949
BarI GAAGNNNNNNTAC 2 cut(s) 10, 42
Bbv12I GWGCWC 2 cut(s) 49, 744
BbvI GCAGC 1 cut(s) 135
BccI CCATC 4 cut(s) 102, 488, 555, 718
BceAI ACGGC 2 cut(s) 53, 92
BciT130I CCWGG 6 cut(s) 372, 650, 670, 727, 792, 843
BcoDI GTCTC 1 cut(s) 1122
BfaI CTAG 1 cut(s) 714
BfuAI ACCTGC 2 cut(s) 854, 974
BglII AGATCT 1 cut(s) 146
BisI GCNGC 1 cut(s) 124
BlpI GCTNAGC 1 cut(s) 919
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 6 cut(s) 372, 650, 670, 727, 792, 843
Bme18I GGWCC 5 cut(s) 91, 190, 266, 374, 1158
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 6 cut(s) 91, 190, 266, 374, 878, 1158
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 6 cut(s) 372, 650, 670, 727, 792, 843
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 55
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 954
Bpu1102I GCTNAGC 1 cut(s) 919
BpuEI CTTGAG 2 cut(s) 758, 1138
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 1 cut(s) 1135
BsaJI CCNNGG 3 cut(s) 544, 881, 941
BsaWI WCCGGW 1 cut(s) 508
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse118I RCCGGY 2 cut(s) 660, 1002
Bse1I ACTGG 2 cut(s) 66, 240
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 6 cut(s) 372, 650, 670, 727, 792, 843
BseDI CCNNGG 3 cut(s) 544, 881, 941
BseGI GGATG 6 cut(s) 70, 94, 288, 481, 499, 566
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMII CTCAG 3 cut(s) 287, 447, 933
BseNI ACTGG 2 cut(s) 66, 240
BseRI GAGGAG 1 cut(s) 561
BseXI GCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 882
BshFI GGCC 2 cut(s) 412, 880
BsiHKAI GWGCWC 2 cut(s) 49, 744
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 3 cut(s) 509, 661, 1003
BslFI GGGAC 2 cut(s) 824, 1044
BslI CCNNNNNNNGG 1 cut(s) 848
BsmAI GTCTC 1 cut(s) 1122
BsmFI GGGAC 2 cut(s) 824, 1044
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 2 cut(s) 412, 880
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 3 cut(s) 49, 744, 949
Bsp143I GATC 4 cut(s) 146, 280, 950, 1168
Bsp1720I GCTNAGC 1 cut(s) 919
BspACI CCGC 2 cut(s) 98, 976
BspANI GGCC 2 cut(s) 412, 880
BspCNI CTCAG 3 cut(s) 288, 448, 932
BspLI GGNNCC 1 cut(s) 550
BspMI ACCTGC 2 cut(s) 854, 974
BspPI GGATC 1 cut(s) 1176
BsrFI RCCGGY 2 cut(s) 660, 1002
BsrI ACTGG 2 cut(s) 66, 240
BssAI RCCGGY 2 cut(s) 660, 1002
BssECI CCNNGG 3 cut(s) 544, 881, 941
BssMI GATC 4 cut(s) 146, 280, 950, 1168
BssNI GRCGYC 1 cut(s) 1135
BssT1I CCWWGG 2 cut(s) 881, 941
Bst2UI CCWGG 6 cut(s) 372, 650, 670, 727, 792, 843
Bst4CI ACNGT 5 cut(s) 194, 227, 260, 266, 929
BstACI GRCGYC 1 cut(s) 1135
BstC8I GCNNGC 2 cut(s) 662, 666
BstDEI CTNAG 3 cut(s) 296, 456, 919
BstF5I GGATG 6 cut(s) 70, 94, 288, 481, 499, 566
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 4 cut(s) 149, 283, 953, 1171
BstMAI GTCTC 1 cut(s) 1122
BstMBI GATC 4 cut(s) 146, 280, 950, 1168
BstMWI GCNNNNNNNGC 5 cut(s) 84, 531, 540, 869, 929
BstNI CCWGG 6 cut(s) 372, 650, 670, 727, 792, 843
BstNSI RCATGY 3 cut(s) 392, 668, 1093
BstSCI CCNGG 6 cut(s) 370, 648, 668, 725, 790, 841
BstV1I GCAGC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 412, 880
BtsCI GGATG 6 cut(s) 70, 94, 288, 481, 499, 566
BveI ACCTGC 2 cut(s) 854, 974
Cac8I GCNNGC 2 cut(s) 662, 666
CfoI GCGC 1 cut(s) 660
Cfr10I RCCGGY 2 cut(s) 660, 1002
Cfr13I GGNCC 6 cut(s) 91, 190, 266, 374, 878, 1158
CseI GACGC 1 cut(s) 1124
CsiI ACCWGGT 1 cut(s) 841
DdeI CTNAG 3 cut(s) 296, 456, 919
DpnI GATC 4 cut(s) 148, 282, 952, 1170
DpnII GATC 4 cut(s) 146, 280, 950, 1168
DraI TTTAAA 3 cut(s) 199, 405, 993
Eco130I CCWWGG 2 cut(s) 881, 941
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 949
Eco47I GGWCC 5 cut(s) 91, 190, 266, 374, 1158
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 618
EcoRII CCWGG 6 cut(s) 370, 648, 668, 725, 790, 841
EcoT14I CCWWGG 2 cut(s) 881, 941
EcoT38I GRGCYC 1 cut(s) 949
ErhI CCWWGG 2 cut(s) 881, 941
FaqI GGGAC 2 cut(s) 824, 1044
FblI GTMKAC 1 cut(s) 1138
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 6 cut(s) 77, 81, 275, 488, 506, 573
FriOI GRGCYC 1 cut(s) 949
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 1 cut(s) 714
GlaI GCGC 1 cut(s) 659
GluI GCNGC 1 cut(s) 124
GsuI CTGGAG 1 cut(s) 954
HaeIII GGCC 2 cut(s) 412, 880
HapII CCGG 3 cut(s) 509, 661, 1003
HgaI GACGC 1 cut(s) 1124
HhaI GCGC 1 cut(s) 660
Hin1I GRCGYC 1 cut(s) 1135
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HincII GTYRAC 1 cut(s) 1139
HindII GTYRAC 1 cut(s) 1139
HinfI GANTC 6 cut(s) 32, 305, 452, 458, 512, 623
HpaII CCGG 3 cut(s) 509, 661, 1003
HphI GGTGA 5 cut(s) 498, 671, 770, 878, 1028
Hpy166II GTNNAC 3 cut(s) 679, 888, 1139
Hpy188I TCNGA 8 cut(s) 37, 280, 285, 297, 457, 700, 909, 1162
Hpy188III TCNNGA 5 cut(s) 398, 467, 620, 714, 1037
Hpy8I GTNNAC 3 cut(s) 679, 888, 1139
Hpy99I CGWCG 2 cut(s) 1140, 1143
HpyAV CCTTC 2 cut(s) 425, 1125
HpyCH4III ACNGT 5 cut(s) 194, 227, 260, 266, 929
HpyCH4IV ACGT 1 cut(s) 1141
HpyCH4V TGCA 4 cut(s) 87, 123, 144, 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 531, 540, 869, 929
HpyF3I CTNAG 3 cut(s) 296, 456, 919
HpySE526I ACGT 1 cut(s) 1141
Hsp92I GRCGYC 1 cut(s) 1135
HspAI GCGC 1 cut(s) 658
KroI GCCGGC 1 cut(s) 660
KroNI GCCGGC 1 cut(s) 662
Kzo9I GATC 4 cut(s) 146, 280, 950, 1168
LmnI GCTCC 1 cut(s) 548
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 1 cut(s) 55
MabI ACCWGGT 1 cut(s) 841
MaeI CTAG 1 cut(s) 714
MaeII ACGT 1 cut(s) 1141
MaeIII GTNAC 5 cut(s) 167, 260, 326, 513, 641
MalI GATC 4 cut(s) 148, 282, 952, 1170
MboI GATC 4 cut(s) 146, 280, 950, 1168
MboII GAAGA 3 cut(s) 30, 587, 613
MfeI CAATTG 1 cut(s) 518
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 3 cut(s) 49, 744, 949
MluCI AATT 7 cut(s) 181, 346, 364, 502, 518, 752, 1021
MlyI GAGTC 3 cut(s) 299, 467, 521
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 9 cut(s) 279, 303, 387, 539, 559, 706, 842, 1063, 1159
MroNI GCCGGC 1 cut(s) 660
MseI TTAA 6 cut(s) 51, 198, 363, 404, 594, 992
MslI CAYNNNNRTG 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 3 cut(s) 509, 661, 1003
MspR9I CCNGG 6 cut(s) 372, 650, 670, 727, 792, 843
MunI CAATTG 1 cut(s) 518
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 6 cut(s) 372, 650, 670, 727, 792, 843
MwoI GCNNNNNNNGC 5 cut(s) 84, 531, 540, 869, 929
NaeI GCCGGC 1 cut(s) 662
NdeII GATC 4 cut(s) 146, 280, 950, 1168
NgoMIV GCCGGC 1 cut(s) 660
NlaIV GGNNCC 1 cut(s) 550
NmeAIII GCCGAG 1 cut(s) 569
NmuCI GTSAC 2 cut(s) 326, 513
NspI RCATGY 3 cut(s) 392, 668, 1093
PaeI GCATGC 1 cut(s) 668
PaqCI CACCTGC 2 cut(s) 854, 974
PceI AGGCCT 1 cut(s) 412
PciI ACATGT 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 727
PctI GAATGC 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 662
PfeI GAWTC 3 cut(s) 32, 452, 623
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 3 cut(s) 299, 466, 520
PpsI GAGTC 3 cut(s) 299, 466, 520
PscI ACATGT 1 cut(s) 388
Psp6I CCWGG 6 cut(s) 370, 648, 668, 725, 790, 841
PspGI CCWGG 6 cut(s) 370, 648, 668, 725, 790, 841
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 6 cut(s) 91, 190, 266, 374, 878, 1158
PsuI RGATCY 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 923
RseI CAYNNNNRTG 1 cut(s) 1094
SalI GTCGAC 1 cut(s) 1137
SaqAI TTAA 6 cut(s) 51, 198, 363, 404, 594, 992
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 4 cut(s) 146, 280, 950, 1168
Sau96I GGNCC 6 cut(s) 91, 190, 266, 374, 878, 1158
SchI GAGTC 3 cut(s) 299, 467, 521
ScrFI CCNGG 6 cut(s) 372, 650, 670, 727, 792, 843
SduI GDGCHC 3 cut(s) 49, 744, 949
SexAI ACCWGGT 1 cut(s) 841
SfaNI GCATC 1 cut(s) 55
SgrDI CGTCGACG 1 cut(s) 1137
SinI GGWCC 5 cut(s) 91, 190, 266, 374, 1158
SmiI ATTTAAAT 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 2 cut(s) 737, 1153
SmoI CTYRAG 2 cut(s) 737, 1153
SphI GCATGC 1 cut(s) 668
Sse9I AATT 7 cut(s) 181, 346, 364, 502, 518, 752, 1021
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 2 cut(s) 98, 976
SspMI CTAG 1 cut(s) 714
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 6 cut(s) 370, 648, 668, 725, 790, 841
StyI CCWWGG 2 cut(s) 881, 941
SwaI ATTTAAAT 1 cut(s) 405
TaaI ACNGT 5 cut(s) 194, 227, 260, 266, 929
TaiI ACGT 1 cut(s) 1144
TaqI TCGA 3 cut(s) 949, 1138, 1171
TasI AATT 7 cut(s) 181, 346, 364, 502, 518, 752, 1021
TfiI GAWTC 3 cut(s) 32, 452, 623
Tru1I TTAA 6 cut(s) 51, 198, 363, 404, 594, 992
Tru9I TTAA 6 cut(s) 51, 198, 363, 404, 594, 992
TseFI GTSAC 2 cut(s) 326, 513
TseI GCWGC 1 cut(s) 123
Tsp45I GTSAC 2 cut(s) 326, 513
TspDTI ATGAA 7 cut(s) 63, 236, 327, 336, 492, 767, 1011
VpaK11BI GGWCC 5 cut(s) 91, 190, 266, 374, 1158
XapI RAATTY 3 cut(s) 502, 752, 1021
XbaI TCTAGA 1 cut(s) 713
XceI RCATGY 3 cut(s) 392, 668, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 1138
XspI CTAG 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.