Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

16115498 .. 16121534

Gene Structure 6037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G44530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3177 bp

ATGATTGCGGAGAAGCCCTTTTGGGTTAAACATGAAGGGTTACAGATATTCTCCATTGATGTTCAACCTAATGGAGAAAGGTTTGCTACTGGCGGTGGTGACCACAAGGTTCGTATATGGAACATGAAGTCTGTTGACAAGGATTTGCAAAACATAGATACAAAAGAGAGGCTTCTTGCAACTCTTCGTGACCATTTTGGGTCAGTCAACTGTGTTAGGTGGGCCAAGAACAGTAGGTATGTAGCATCTGGTTCTGATGATCAAGTGATTCAAATCCATGAGAGGAAGCCTGGTTCAGGCACCACTGAGTTTGGTAGTGGAGAAGCCCCTGATGTTGAGAACTGGAAAGCTGTAATGACTTTAAGGGGACACACTGCCGATGTGGTGGATCTTAATTGGTCTCCAGATGATTCGATGCTGGCTAGTGGGAGTTTGGACAACACAGTGCATATATGGAATATGCGCACTGGTATGTGCACCACTGTTCTCAGGGGTCACTTGAGTTTGGTTAAAGGTGTAACTTGGGATCCAATTGGGTCATTTATTGCTAGCCAGTCGGATGACAAAACTGTGATAATATGGCGAACAAGTGATTGGGGCATGGCTCACAGAACAGATGGTCACTGGGCAAAATCGCTTGGATCCACATTTTTCAGGCGACTTGGGTGGTCACCCTGTGGCCACTTCCTTACTACCACTCATGGGTTTCAGAAGCCGAAGCATTCTGCTCCTGTTTTAGAAAGAGGGGAATGGTCTGTAGCATATGACTTCTTAGGTCATAGTGCTCCAATCATTGTTGTTAGATTCAATCATTCAATGTTCAAAAGAATCCCCTCTAGTACCCATGAAACTAAACAAGTTGGGTGGAGCAATGGAACATCAAAGTCAGGGGAGAAAGATTTGCAGTCATACAATGTTATTGCAATGGGGAGTCAAGACCGTACTATAACCGTATGGACAACTGGAAGTGCGCGTCCTCTTTTTGTCGCCAAGCATTTCTTTGGCCAAAGTGTTGTGGATCTATCATGGAGTCCCGACGGATATTCACTTTTCGCATGTTCCTTGGACGGAACGGTGGCGATGATTCACTTTGACCCAAAAGAACTAGGCGTCAGGCTAACCGATACTGAACTCGACGAATTGAAGAAATCTCGATATGGAGATGTCAGAGGCCGGCAAGCAAACTTAGTTGAGAGCCCAGCCCAGTTATTACTTGAAACAGCCTCGACAAAGCAAGCCGGTAGTAAGAGGGCGGCATCGGATGTTCAACAAAATCAGGTGACTACAAAACCATCTGTTAGTGTGGAGAGTACTGCAAAGAGGCGTAAATCGCAAGTCGATGATCGGAATAAGGCAGCAGAGTCTACTGGTCAAACATTAAATAAAGCATCAACCTTAAATCGAGTATCTAGTCCTGTGAATCAAAAAGTGTATAGAAGGCCTGACGGAAGAAAGAGGATCATTCCTGAAGCTGTTGGAGTTCCCCAACAGGAGAATAATATCATGATTAATGGGGAATCACATAATTTTCTGCCTGCTTCCGCTGCTGCTCCTGCTAAGGGAGATAGTGGAGATTTTCCTGTGGAGATTAGCAATAGGGATTTGTCCGGGAAAGAAATAGTTTGCAGGAACCCTGATCTGAAAGAGCGTTCAAGGATTACAGCTCGTGCTACCATTACTGAGAGCCTTGTTATTGAGAAGGTTCCTGGAACCTCTGGTAGGGATGGAGTTTTAAATGTTGAGCAATCTGTAGGTATAAAAGAATCTTCTAGCACTGATCTGTTGATAAGGGTATTCGATTGGAAAGATGGGGAAGCTGCACCACCTGTTTGCTTAGAAGCTTGTCCAAGGGAGCATGCTTTGGACACTGTTGGTGCAGTAAGTACATCAATGGTCAAGGAAACTGAGATTTCTTGCAAAAAGTCAGGCGAAACTCTTTGGTCTGATCGGATTATGGGGAGAGTCACTGTTTTGGCTGGAAATCCAAATTTTTGGGCTGCTGGATGCGAAGACGGAAGCCTCCAGGTTTACACTAAGTGTGGAAGGCGTGCTATGCCTACAATGATGATGGGGTCTGCTGCAACTTTTATTGATTGTGACGATAGCTGGAAGTTGTTGCTTGTAACAAGAAAAGGATCTCTATATGTATGGGATCTATTCAATAGGAAATGCGTTCTTCACGACTCTTTATCTTCTTTGGTCTCTTCCGATGTCAATTTATCTTCCACAGTGAAAGTAAAAAGAATGAAGAACTGGTATTTACTAATTTTCCTCGTGGGTGACTTGGTAGTGGAGTTACTATCTTGGTATGAAGATAGCTCTGTTATTGATTTGGACTGTACAATAAAAGTGATATCTGTGAAATTATCCAAATCTGGTTCACCGCTTGTTGTTCTAGCCACACGTCATGCATTCCTTTTTGACACTAGTCTAATGTGTTGGCTGAGAGTGGCTGATGACTGCTTCCCGGCCTCAAATTTTAGTAGCTCCTGGAATTTGGGCTCAGCTCCATGCGGCGAGCTTGCTGGTTTGCAGGTTGATGTCAGAAAGTACATGGCTAGAAAGCCAGGGTGGAACAGGATAACTGATGATGGAACGCAAACACGTGCACATTTGGAATCTCAACTTGCATCTTCTCTTGCACTTGAGTCTCCTAATGAATACCGCCAGTGCCTCCTTGCCTATGTGAGGTTTCTAGCAAGAGAGGCAGACGAGTCCAGATTAAGAGAAGTCTGTGAGAGCTTTCTTGGACCTCCCACTGGTATGGCTGAAGCAGCATCTTCAGACACAAACTTGTCTTGGGATCCTTATGTTCTGGGGGTGAAGAAGCATAAACTTTTGAGAAACGATATTCTACCTGCAATGGCGTCAAACAGAAAAGTGCAGCGTCTACTCAATGAGTTTATAGATCTCTTATCTGAATATGAAGACGTAGAAACAGCAGATCCTGCCCCCAAGGGCTCGACACCTACCATGAACTGTGGTGGAGTCCCATCTTCACTGGATCAAATTGGCTCTGATCCTCCAGCCATGACAGCTACTACTCCTATGACTATAGACAATGACAAACCAGTCTCTCTGGAGAATCCAGCGGCTTTGGATATAGGTGTGTGTGAGAAAACGGGTTCTGAGGACCGGGATAAACAAGACCAGAACTCAAGAGACTCGGGCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1058

Amino Acids

116.49

Weight (kDa)

6.4

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 369	2.5e-54	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	12 - 98	9.9e-08	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	13 - 92	7.9e-07	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	15 - 198	1.1e-34	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	16 - 97	1.7e-09	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 199	3.9e-17	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	51 - 197	2.5e-07	MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz	PF24807	51 - 262	1.8e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	58 - 92	2.9e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	66 - 271	1.1e-25	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	5.5e-08	WDR35/TULP4 N-terminal
WD40_WDHD1_1st	PF24817	69 - 232	7.2e-14	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	111 - 211	2e-17	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	111 - 195	3.3e-13	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 194	7.9e-08	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	111 - 193	1.5e-11	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	113 - 203	4.6e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 210	9.4e-09	WDR3 second beta-propeller domain
WD40	PF00400	116 - 153	3e-10	WD domain, G-beta repeat
Beta-prop_Aladin	PF25460	117 - 232	3e-08	Aladin seven-bladed propeller
WD40_WDHD1_1st	PF24817	120 - 258	1.8e-14	WDHD1 first WD40 domain
Hira	PF07569	681 - 743	2.5e-14	TUP1-like enhancer of split
Hira	PF07569	778 - 914	1.4e-30	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3071
Acc16I	TGCGCA	1 cut(s)	464
Acc36I	ACCTGC	2 cut(s)	2524, 2866
AccB1I	GGYRCC	1 cut(s)	299
AccI	GTMKAC	2 cut(s)	1364, 2890
AccII	CGCG	1 cut(s)	973
AciI	CCGC	8 cut(s)	8, 93, 1253, 1542, 2384, 2514, 2665, 3092
AcoI	YGGCCR	2 cut(s)	679, 1003
AcsI	RAATTY	3 cut(s)	1987, 2476, 2494
AcuI	CTGAAG	3 cut(s)	1488, 2766, 2790
AcvI	CACGTG	1 cut(s)	2606
AcyI	GRCGYC	2 cut(s)	1110, 2867
AdeI	CACNNNGTG	2 cut(s)	677, 2037
AfaI	GTAC	6 cut(s)	841, 943, 1312, 1885, 2341, 2552
AfiI	CCNNNNNNNGG	6 cut(s)	296, 702, 1559, 1719, 2688, 2759
AflIII	ACRYGT	2 cut(s)	2402, 2603
AhlI	ACTAGT	1 cut(s)	2426
AjiI	CACGTC	1 cut(s)	2405
AjnI	CCWGG	5 cut(s)	289, 1705, 2022, 2489, 2566
AjuI	GAANNNNNNNTTGG	2 cut(s)	577, 609
AloI	GAACNNNNNNTCC	2 cut(s)	277, 309
Alw21I	GWGCWC	3 cut(s)	479, 787, 2611
Alw26I	GTCTC	5 cut(s)	405, 2206, 2655, 3079, 3156
Alw44I	GTGCAC	2 cut(s)	475, 2607
AlwNI	CAGNNNCTG	1 cut(s)	2948
Ama87I	CYCGRG	1 cut(s)	3166
AoxI	GGCC	6 cut(s)	222, 679, 1003, 1171, 1439, 2469
ApaLI	GTGCAC	2 cut(s)	475, 2607
ApeKI	GCWGC	8 cut(s)	1355, 1544, 1547, 1817, 1997, 2078, 2774, 2884
ApoI	RAATTY	3 cut(s)	1987, 2476, 2494
AseI	ATTAAT	1 cut(s)	1509
AspLEI	GCGC	2 cut(s)	465, 973
AspS9I	GGNCC	3 cut(s)	222, 2750, 3133
AsuC2I	CCSGG	3 cut(s)	1609, 2468, 3137
AsuHPI	GGTGA	6 cut(s)	110, 663, 1291, 2291, 2373, 2833
AsuNHI	GCTAGC	1 cut(s)	548
AvaI	CYCGRG	1 cut(s)	3166
AvaII	GGWCC	2 cut(s)	2750, 3133
BaeGI	GKGCMC	2 cut(s)	479, 2611
BalI	TGGCCA	2 cut(s)	681, 1005
BamHI	GGATCC	3 cut(s)	526, 641, 2803
BanI	GGYRCC	1 cut(s)	299
BanII	GRGCYC	4 cut(s)	1199, 2504, 2963, 3173
BauI	CACGAG	2 cut(s)	1665, 2273
BbrPI	CACGTG	1 cut(s)	2606
BbsI	GAAGAC	2 cut(s)	2016, 2934
Bbv12I	GWGCWC	3 cut(s)	479, 787, 2611
BbvI	GCAGC	8 cut(s)	1367, 1531, 1534, 1804, 1984, 2065, 2786, 2896
BccI	CCATC	7 cut(s)	611, 1300, 1718, 1802, 2062, 2585, 3001
BciT130I	CCWGG	5 cut(s)	291, 1707, 2024, 2491, 2568
BclI	TGATCA	1 cut(s)	259
BcnI	CCSGG	3 cut(s)	1609, 2468, 3137
BcoDI	GTCTC	5 cut(s)	405, 2206, 2655, 3079, 3156
BcuI	ACTAGT	1 cut(s)	2426
BfmI	CTRYAG	3 cut(s)	756, 1749, 3054
BfuAI	ACCTGC	2 cut(s)	2524, 2866
BglII	AGATCT	1 cut(s)	2908
BlpI	GCTNAGC	1 cut(s)	2503
BmcAI	AGTACT	1 cut(s)	1312
Bme1390I	CCNGG	8 cut(s)	291, 1609, 1707, 2024, 2468, 2491, 2568, 3137
Bme18I	GGWCC	2 cut(s)	2750, 3133
BmeT110I	CYCGRG	1 cut(s)	3166
BmgBI	CACGTC	1 cut(s)	2405
BmgT120I	GGNCC	3 cut(s)	222, 2750, 3133
BmiI	GGNNCC	7 cut(s)	301, 528, 643, 1631, 1704, 1711, 2805
BmrFI	CCNGG	8 cut(s)	291, 1609, 1707, 2024, 2468, 2491, 2568, 3137
BmrI	ACTGGG	2 cut(s)	634, 1198
BmsI	GCATC	7 cut(s)	254, 405, 1265, 1397, 1994, 2639, 2786
BmtI	GCTAGC	1 cut(s)	552
BmuI	ACTGGG	2 cut(s)	634, 1198
BoxI	GACNNNNGTC	1 cut(s)	2427
BpiI	GAAGAC	2 cut(s)	2016, 2934
BpmI	CTGGAG	4 cut(s)	387, 2006, 3009, 3101
Bpu10I	CCTNAGC	1 cut(s)	1557
Bpu1102I	GCTNAGC	1 cut(s)	2503
BpuEI	CTTGAG	3 cut(s)	520, 2666, 3142
BpuMI	CCSGG	3 cut(s)	1609, 2468, 3137
BsaAI	YACGTR	1 cut(s)	2606
BsaBI	GATNNNNATC	1 cut(s)	272
BsaHI	GRCGYC	2 cut(s)	1110, 2867
BsaI	GGTCTC	2 cut(s)	405, 2206
BsaJI	CCNNGG	4 cut(s)	1062, 1847, 2567, 2955
Bsc4I	CCNNNNNNNGG	6 cut(s)	296, 702, 1559, 1719, 2688, 2759
Bse118I	RCCGGY	2 cut(s)	1173, 1238
Bse3DI	GCAATG	3 cut(s)	877, 930, 2868
Bse8I	GATNNNNATC	1 cut(s)	272
BseBI	CCWGG	5 cut(s)	291, 1707, 2024, 2491, 2568
BseDI	CCNNGG	4 cut(s)	1062, 1847, 2567, 2955
BseGI	GGATG	4 cut(s)	565, 1267, 1729, 2009
BseJI	GATNNNNATC	1 cut(s)	272
BseLI	CCNNNNNNNGG	6 cut(s)	296, 702, 1559, 1719, 2688, 2759
BseMI	GCAATG	3 cut(s)	877, 930, 2868
BseMII	CTCAG	7 cut(s)	297, 502, 1671, 1896, 2435, 2517, 3120
BseSI	GKGCMC	2 cut(s)	479, 2611
BseXI	GCAGC	8 cut(s)	1367, 1531, 1534, 1804, 1984, 2065, 2786, 2896
BseYI	CCCAGC	1 cut(s)	1198
BsgI	GTGCAG	3 cut(s)	1803, 1896, 2903
Bsh1236I	CGCG	1 cut(s)	973
BshFI	GGCC	6 cut(s)	224, 681, 1005, 1173, 1441, 2471
BshNI	GGYRCC	1 cut(s)	299
BsiHKAI	GWGCWC	3 cut(s)	479, 787, 2611
BsiHKCI	CYCGRG	1 cut(s)	3166
BsiSI	CCGG	5 cut(s)	1174, 1239, 1608, 2468, 3136
BslFI	GGGAC	3 cut(s)	381, 1017, 2975
BslI	CCNNNNNNNGG	6 cut(s)	296, 702, 1559, 1719, 2688, 2759
BsmAI	GTCTC	5 cut(s)	405, 2206, 2655, 3079, 3156
BsmFI	GGGAC	3 cut(s)	381, 1017, 2975
BsmI	GAATGC	2 cut(s)	721, 2411
BsnI	GGCC	6 cut(s)	224, 681, 1005, 1173, 1441, 2471
Bso31I	GGTCTC	2 cut(s)	405, 2206
BsoBI	CYCGRG	1 cut(s)	3166
Bsp1286I	GDGCHC	7 cut(s)	479, 787, 1199, 2504, 2611, 2963, 3173
Bsp1407I	TGTACA	1 cut(s)	2339
Bsp1720I	GCTNAGC	1 cut(s)	2503
BspACI	CCGC	8 cut(s)	8, 93, 1253, 1542, 2384, 2514, 2665, 3092
BspANI	GGCC	6 cut(s)	224, 681, 1005, 1173, 1441, 2471
BspCNI	CTCAG	7 cut(s)	298, 501, 1672, 1897, 2436, 2516, 3121
BspFNI	CGCG	1 cut(s)	973
BspHI	TCATGA	1 cut(s)	1503
BspLI	GGNNCC	7 cut(s)	301, 528, 643, 1631, 1704, 1711, 2805
BspMI	ACCTGC	2 cut(s)	2524, 2866
BspOI	GCTAGC	1 cut(s)	552
BspT107I	GGYRCC	1 cut(s)	299
BspTNI	GGTCTC	2 cut(s)	405, 2206
BsrDI	GCAATG	3 cut(s)	877, 930, 2868
BsrFI	RCCGGY	2 cut(s)	1173, 1238
BsrGI	TGTACA	1 cut(s)	2339
BssAI	RCCGGY	2 cut(s)	1173, 1238
BssECI	CCNNGG	4 cut(s)	1062, 1847, 2567, 2955
BssNI	GRCGYC	2 cut(s)	1110, 2867
BssSI	CACGAG	2 cut(s)	1665, 2273
BssT1I	CCWWGG	3 cut(s)	1062, 1847, 2955
Bst2BI	CACGAG	2 cut(s)	1665, 2273
Bst2UI	CCWGG	5 cut(s)	291, 1707, 2024, 2491, 2568
Bst6I	CTCTTC	2 cut(s)	189, 2209
BstACI	GRCGYC	2 cut(s)	1110, 2867
BstAPI	GCANNNNNTGC	1 cut(s)	2948
BstAUI	TGTACA	1 cut(s)	2339
BstBAI	YACGTR	1 cut(s)	2606
BstEII	GGTNACC	2 cut(s)	98, 669
BstENI	CCTNNNNNAGG	3 cut(s)	294, 1717, 2686
BstF5I	GGATG	4 cut(s)	565, 1267, 1729, 2009
BstFNI	CGCG	1 cut(s)	973
BstHHI	GCGC	2 cut(s)	465, 973
BstMAI	GTCTC	5 cut(s)	405, 2206, 2655, 3079, 3156
BstMWI	GCNNNNNNNGC	8 cut(s)	1330, 1544, 1553, 2053, 2705, 2774, 2948, 3035
BstNI	CCWGG	5 cut(s)	291, 1707, 2024, 2491, 2568
BstNSI	RCATGY	2 cut(s)	1059, 1859
BstPAI	GACNNNNGTC	1 cut(s)	2427
BstPI	GGTNACC	2 cut(s)	98, 669
BstSCI	CCNGG	8 cut(s)	289, 1607, 1705, 2022, 2466, 2489, 2566, 3135
BstSFI	CTRYAG	3 cut(s)	756, 1749, 3054
BstSLI	GKGCMC	2 cut(s)	479, 2611
BstUI	CGCG	1 cut(s)	973
BstV1I	GCAGC	8 cut(s)	1367, 1531, 1534, 1804, 1984, 2065, 2786, 2896
BstV2I	GAAGAC	2 cut(s)	2016, 2934
BstX2I	RGATCY	9 cut(s)	388, 526, 641, 1018, 2135, 2152, 2803, 2908, 2944
BstXI	CCANNNNNNTGG	2 cut(s)	1992, 2764
BstYI	RGATCY	9 cut(s)	388, 526, 641, 1018, 2135, 2152, 2803, 2908, 2944
BsuRI	GGCC	6 cut(s)	224, 681, 1005, 1173, 1441, 2471
BtgZI	GCGATG	1 cut(s)	1094
BtrI	CACGTC	1 cut(s)	2405
BtsCI	GGATG	4 cut(s)	565, 1267, 1729, 2009
BtsI	GCAGTG	1 cut(s)	372
BveI	ACCTGC	2 cut(s)	2524, 2866
CaiI	CAGNNNCTG	1 cut(s)	2948
CciI	TCATGA	1 cut(s)	1503
CfoI	GCGC	2 cut(s)	465, 973
Cfr10I	RCCGGY	2 cut(s)	1173, 1238
Cfr13I	GGNCC	3 cut(s)	222, 2750, 3133
CseI	GACGC	4 cut(s)	962, 1099, 2856, 2876
Csp6I	GTAC	6 cut(s)	840, 942, 1311, 1884, 2340, 2551
CspCI	CAANNNNNGTGG	4 cut(s)	292, 327, 845, 880
CviQI	GTAC	6 cut(s)	840, 942, 1311, 1884, 2340, 2551
DraI	TTTAAA	1 cut(s)	1734
DraIII	CACNNNGTG	2 cut(s)	677, 2037
DrdI	GACNNNNNNGTC	1 cut(s)	3071
DseDI	GACNNNNNNGTC	1 cut(s)	3071
EaeI	YGGCCR	2 cut(s)	679, 1003
Eam1104I	CTCTTC	2 cut(s)	189, 2209
EarI	CTCTTC	2 cut(s)	189, 2209
Eco130I	CCWWGG	3 cut(s)	1062, 1847, 2955
Eco147I	AGGCCT	1 cut(s)	1441
Eco24I	GRGCYC	4 cut(s)	1199, 2504, 2963, 3173
Eco31I	GGTCTC	2 cut(s)	405, 2206
Eco32I	GATATC	1 cut(s)	2355
Eco47I	GGWCC	2 cut(s)	2750, 3133
Eco57I	CTGAAG	3 cut(s)	1488, 2766, 2790
Eco72I	CACGTG	1 cut(s)	2606
Eco88I	CYCGRG	1 cut(s)	3166
Eco91I	GGTNACC	2 cut(s)	98, 669
EcoNI	CCTNNNNNAGG	3 cut(s)	294, 1717, 2686
EcoO65I	GGTNACC	2 cut(s)	98, 669
EcoRII	CCWGG	5 cut(s)	289, 1705, 2022, 2489, 2566
EcoRV	GATATC	1 cut(s)	2355
EcoT14I	CCWWGG	3 cut(s)	1062, 1847, 2955
EcoT22I	ATGCAT	1 cut(s)	2413
EcoT38I	GRGCYC	4 cut(s)	1199, 2504, 2963, 3173
ErhI	CCWWGG	3 cut(s)	1062, 1847, 2955
FalI	AAGNNNNNCTT	4 cut(s)	156, 188, 983, 1015
FaqI	GGGAC	3 cut(s)	381, 1017, 2975
FauNDI	CATATG	1 cut(s)	763
FbaI	TGATCA	1 cut(s)	259
FblI	GTMKAC	2 cut(s)	1364, 2890
FokI	GGATG	4 cut(s)	572, 1274, 1736, 2016
FriOI	GRGCYC	4 cut(s)	1199, 2504, 2963, 3173
FspAI	RTGCGCAY	1 cut(s)	464
FspI	TGCGCA	1 cut(s)	464
GlaI	GCGC	2 cut(s)	464, 972
GsaI	CCCAGC	1 cut(s)	1202
GsuI	CTGGAG	4 cut(s)	387, 2006, 3009, 3101
HaeIII	GGCC	6 cut(s)	224, 681, 1005, 1173, 1441, 2471
HapII	CCGG	5 cut(s)	1174, 1239, 1608, 2468, 3136
HgaI	GACGC	4 cut(s)	962, 1099, 2856, 2876
HhaI	GCGC	2 cut(s)	465, 973
Hin1I	GRCGYC	2 cut(s)	1110, 2867
Hin6I	GCGC	2 cut(s)	463, 971
HinP1I	GCGC	2 cut(s)	463, 971
HincII	GTYRAC	2 cut(s)	136, 208
HindII	GTYRAC	2 cut(s)	136, 208
HindIII	AAGCTT	1 cut(s)	1839
HpaII	CCGG	5 cut(s)	1174, 1239, 1608, 2468, 3136
HphI	GGTGA	6 cut(s)	110, 663, 1291, 2291, 2373, 2833
Hpy166II	GTNNAC	8 cut(s)	136, 208, 477, 1365, 2029, 2381, 2609, 2891
Hpy8I	GTNNAC	8 cut(s)	136, 208, 477, 1365, 2029, 2381, 2609, 2891
Hpy99I	CGWCG	2 cut(s)	1040, 1139
HpyAV	CCTTC	4 cut(s)	29, 1431, 1693, 2037
HpyCH4IV	ACGT	3 cut(s)	2404, 2605, 2931
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1330, 1544, 1553, 2053, 2705, 2774, 2948, 3035
HpySE526I	ACGT	3 cut(s)	2404, 2605, 2931
Hsp92I	GRCGYC	2 cut(s)	1110, 2867
HspAI	GCGC	2 cut(s)	463, 971
KroI	GCCGGC	1 cut(s)	1173
KroNI	GCCGGC	1 cut(s)	1175
Ksp22I	TGATCA	1 cut(s)	259
LmnI	GCTCC	7 cut(s)	733, 790, 867, 1555, 1852, 2492, 2512
Lsp1109I	GCAGC	8 cut(s)	1367, 1531, 1534, 1804, 1984, 2065, 2786, 2896
LweI	GCATC	7 cut(s)	254, 405, 1265, 1397, 1994, 2639, 2786
MaeII	ACGT	3 cut(s)	2404, 2605, 2931
MfeI	CAATTG	1 cut(s)	531
MflI	RGATCY	9 cut(s)	388, 526, 641, 1018, 2135, 2152, 2803, 2908, 2944
MhlI	GDGCHC	7 cut(s)	479, 787, 1199, 2504, 2611, 2963, 3173
MlsI	TGGCCA	2 cut(s)	681, 1005
MluNI	TGGCCA	2 cut(s)	681, 1005
MlyI	GAGTC	9 cut(s)	940, 1039, 1370, 1971, 2177, 2657, 2723, 2997, 3158
MmeI	TCCRAC	2 cut(s)	537, 1456
Mox20I	TGGCCA	2 cut(s)	681, 1005
Mph1103I	ATGCAT	1 cut(s)	2413
MroNI	GCCGGC	1 cut(s)	1173
MscI	TGGCCA	2 cut(s)	681, 1005
MseI	TTAA	9 cut(s)	27, 362, 393, 510, 1379, 1397, 1509, 1733, 2723
MslI	CAYNNNNRTG	2 cut(s)	470, 2762
Msp20I	TGGCCA	2 cut(s)	681, 1005
MspA1I	CMGCKG	2 cut(s)	1544, 3092
MspI	CCGG	5 cut(s)	1174, 1239, 1608, 2468, 3136
MspR9I	CCNGG	8 cut(s)	291, 1609, 1707, 2024, 2468, 2491, 2568, 3137
MunI	CAATTG	1 cut(s)	531
Mva1269I	GAATGC	2 cut(s)	721, 2411
MvaI	CCWGG	5 cut(s)	291, 1707, 2024, 2491, 2568
MvnI	CGCG	1 cut(s)	973
MwoI	GCNNNNNNNGC	8 cut(s)	1330, 1544, 1553, 2053, 2705, 2774, 2948, 3035
NaeI	GCCGGC	1 cut(s)	1175
NciI	CCSGG	3 cut(s)	1609, 2468, 3137
NdeI	CATATG	1 cut(s)	763
NgoMIV	GCCGGC	1 cut(s)	1173
NheI	GCTAGC	1 cut(s)	548
NlaIV	GGNNCC	7 cut(s)	301, 528, 643, 1631, 1704, 1711, 2805
NmuCI	GTSAC	9 cut(s)	98, 188, 494, 620, 669, 1279, 1963, 2096, 2279
NsbI	TGCGCA	1 cut(s)	464
NsiI	ATGCAT	1 cut(s)	2413
NspI	RCATGY	2 cut(s)	1059, 1859
PaeI	GCATGC	1 cut(s)	1859
PagI	TCATGA	1 cut(s)	1503
PceI	AGGCCT	1 cut(s)	1441
PctI	GAATGC	2 cut(s)	721, 2411
PdiI	GCCGGC	1 cut(s)	1175
PfoI	TCCNGGA	3 cut(s)	1607, 1705, 2489
PleI	GAGTC	9 cut(s)	939, 1038, 1369, 1970, 2177, 2656, 2722, 2996, 3158
PmaCI	CACGTG	1 cut(s)	2606
PmlI	CACGTG	1 cut(s)	2606
PpsI	GAGTC	9 cut(s)	939, 1038, 1369, 1970, 2177, 2656, 2722, 2996, 3158
Ppu21I	YACGTR	1 cut(s)	2606
PshAI	GACNNNNGTC	1 cut(s)	2427
PshBI	ATTAAT	1 cut(s)	1509
Psp6I	CCWGG	5 cut(s)	289, 1705, 2022, 2489, 2566
PspCI	CACGTG	1 cut(s)	2606
PspEI	GGTNACC	2 cut(s)	98, 669
PspFI	CCCAGC	1 cut(s)	1198
PspGI	CCWGG	5 cut(s)	289, 1705, 2022, 2489, 2566
PspN4I	GGNNCC	7 cut(s)	301, 528, 643, 1631, 1704, 1711, 2805
PspPI	GGNCC	3 cut(s)	222, 2750, 3133
PstNI	CAGNNNCTG	1 cut(s)	2948
PsuI	RGATCY	9 cut(s)	388, 526, 641, 1018, 2135, 2152, 2803, 2908, 2944
RsaI	GTAC	6 cut(s)	841, 943, 1312, 1885, 2341, 2552
RsaNI	GTAC	6 cut(s)	840, 942, 1311, 1884, 2340, 2551
RseI	CAYNNNNRTG	2 cut(s)	470, 2762
SaqAI	TTAA	9 cut(s)	27, 362, 393, 510, 1379, 1397, 1509, 1733, 2723
Sau96I	GGNCC	3 cut(s)	222, 2750, 3133
ScaI	AGTACT	1 cut(s)	1312
SchI	GAGTC	9 cut(s)	940, 1039, 1370, 1971, 2177, 2657, 2723, 2997, 3158
ScrFI	CCNGG	8 cut(s)	291, 1609, 1707, 2024, 2468, 2491, 2568, 3137
SduI	GDGCHC	7 cut(s)	479, 787, 1199, 2504, 2611, 2963, 3173
SfaNI	GCATC	7 cut(s)	254, 405, 1265, 1397, 1994, 2639, 2786
SfcI	CTRYAG	3 cut(s)	756, 1749, 3054
SinI	GGWCC	2 cut(s)	2750, 3133
SmiMI	CAYNNNNRTG	2 cut(s)	470, 2762
SmlI	CTYRAG	3 cut(s)	499, 2645, 3157
SmoI	CTYRAG	3 cut(s)	499, 2645, 3157
SpeI	ACTAGT	1 cut(s)	2426
SphI	GCATGC	1 cut(s)	1859
SseBI	AGGCCT	1 cut(s)	1441
SsiI	CCGC	8 cut(s)	8, 93, 1253, 1542, 2384, 2514, 2665, 3092
StuI	AGGCCT	1 cut(s)	1441
StyD4I	CCNGG	8 cut(s)	289, 1607, 1705, 2022, 2466, 2489, 2566, 3135
StyI	CCWWGG	3 cut(s)	1062, 1847, 2955
TaiI	ACGT	3 cut(s)	2407, 2608, 2934
TaqI	TCGA	8 cut(s)	413, 1134, 1153, 1226, 1338, 1402, 1797, 2963
TatI	WGTACW	4 cut(s)	1310, 1883, 2339, 2550
TauI	GCSGC	3 cut(s)	1256, 2517, 3095
Tru1I	TTAA	9 cut(s)	27, 362, 393, 510, 1379, 1397, 1509, 1733, 2723
Tru9I	TTAA	9 cut(s)	27, 362, 393, 510, 1379, 1397, 1509, 1733, 2723
TseFI	GTSAC	9 cut(s)	98, 188, 494, 620, 669, 1279, 1963, 2096, 2279
TseI	GCWGC	8 cut(s)	1355, 1544, 1547, 1817, 1997, 2078, 2774, 2884
Tsp45I	GTSAC	9 cut(s)	98, 188, 494, 620, 669, 1279, 1963, 2096, 2279
TspDTI	ATGAA	8 cut(s)	48, 140, 861, 2261, 2325, 2673, 2940, 2990
TspGWI	ACGGA	4 cut(s)	1053, 1083, 1461, 2028
VneI	GTGCAC	2 cut(s)	475, 2607
VpaK11BI	GGWCC	2 cut(s)	2750, 3133
VspI	ATTAAT	1 cut(s)	1509
XagI	CCTNNNNNAGG	3 cut(s)	294, 1717, 2686
XapI	RAATTY	3 cut(s)	1987, 2476, 2494
XceI	RCATGY	2 cut(s)	1059, 1859
XmiI	GTMKAC	2 cut(s)	1364, 2890
ZrmI	AGTACT	1 cut(s)	1312
Zsp2I	ATGCAT	1 cut(s)	2413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.