Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Reverse (-)

40909751 .. 40919297

Gene Structure 9547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG294700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3021 bp

ATGAAATCTCTTGGCCGGGATATGGAAAATGAGGACTCAACTCAGAGGCTACTTGCAACCCTCCGTGATCACTTTGGTTCTGTTAACTGTGTTAGGTGGGCTAAGCACAGTCGTTATGTTGCAACAGGATCTGATGATCAAGTGATTCTAATTCATGAGAGGAAGCCTGGTTCGGGAACCACCGAGTTTGGCAGTGGAGAGCCCCCTGACGTTGAGAATTGGAAAGTTATAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTTAATTGGTCTCCAGATGACTCGATGTTGGCCAGTGGAAGTTTAGATAATACTATACATATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGTCACTCTAGCCTGGTTAAAGGAGTTACTTGGGATCCCATTGGCTCGTTCATAGCAAGTCAATCAGACGATAAGACAGTAATTATTTGGAAGACAAGCGACTGGAGTCTTGCTCACAGGACTGACGGTCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGGCGTCTTGGATGGTCACCTTGTGGTCACTTCATTACTACTACTCATGGATTCCAGAAACCAAGGCATTCAGCACCTGTTCTTGAGAGAGGCGAATGGTCTGCCACATTTGACTTCCTAGGACATAATGCTCCTATAATCGTTGTGAAGTTTAACCATTCAATGTTTATGAGGAAGTTTACCAATGCTCAGGAAGGGAAAACTGCACCTGTGGGGTGGACTAATGGGGCTTCGAAGACAGGAGGGAAAGAGCCACAACCTTATAATGTTATTGCAATTGGGAGTCAGGACCGGACAATAACTGTATGGACGACTGCAAGCCCACGTCCTCTATTTGTTGCAAAGCATTTCTTTACTCAAAGTGTTGTGGATTTGTCCTGGAGCCCTGATGGTTATTCGCTCTTTGCATGTTCTTTGGATGGTTCGGTGGCTACTTTCCATTTTGAGGTGAAAGAACTTGGTCACCGGTTAAGCGATGCAGAACTAGATGAGTTGAAGAGAAATCGTTATGGTGATGTTAGAGGTCGGCAAGCAAACTTAGCTGAAAGCCCAGCACAGTTATTGCTTGAAGCAGCTTCTGCTAAGCAAGTGGCAAGCAAAAAGGTCTCTCTGGATGTTCAACAAAATCACACACTTGAGAAACCTTCTGCTCATGTGGGGGTTGCAACAAAAGCTTCTGAGACTCAGCTTGCTGATATGAAAAAGAGTGGAGGTGCTGCTCCTGATTGTTTAAATAAAGTTTCGATGGCTACCCGAATATCTGGTCCTGTGAAGCAAAGAGAATATAGACGCCCTGATGGTAGAAAAAGGATTACTCCAGAAGCAGTTGGGGTGCCTTCCCAGCAGGAAAATATCTCTATGGGGGCTCAGTCCCAAGCACTTGACTTCCCTCCCATGTCTTCTGATCATAGGAAGGATGATAATGGTTTAGTTGTTGCTGATAGTGGCATTAGAGAAACTTCTTTCAGGGGAACACTTGGCCGAAACACTGATACAAAGGAAAGATATGGGGCCACTGCCAGAGCTATGATTACTGAAAGTCTGGTTATTGAGAAGGTTCCTGCTTCCACAAGCAGAGATGAAAGCATAAACGTGGAACAAACAGGAAATGTGAAGGCGTGTAACTCAGTAGGCTCTAGTAGTTCTATTCTTTCAATCCGGGTGTTCGATAAGAAAGAATGGGAAGACACAGTGCCAATTTGTTTGGAAGCTCGACCTTGGGAACAAGCTGCAAATGATGTGTTTGGCATGGGAAATACATTTATTACGAAAGAAACTGAAATCACTTGCACAAGGGGGTTGCAAACTCTTTGGTCTGATAGGATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGTAAACTTTTGGGCTGTTGGCTGTGAAGATGGTTGCATACAGGTTTACACTAAGTGTGGGAGACGTGCTATGCCAACAATGATGGTAGGATCTGCTGCAATATTTATAGATTGTGATGAGTGCTGGAAGTTGTTTTTGGTCACAAAAAAAGGATCCTTGTATCTGTGGGATCTATTCACACGGAACTGTCTCCTTAATGACTCCTTGACATCTCTGGTTACTTCAAACCCAAACCCATCTGCAAAAGATGCAGGCATAATCAAAGTTATATCAGCAAAACTATCAAGATCCGGATCACCTATTGTTGTTCTTGCGACTCGTCATGCATTCCTCTTCGACATGGGCCTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTCCTGGGTCAAATTTTGCAAGTTCTTGGAATTTAACTTCAACTCAGAGTGGCGAGCTGGCTGCATTGCAGGTTGATGTTAGGAAATATTTGGCCAGGAAGCCGGTTTGGAGCAGGGTGACTGATGACGGAGTACAGACACGTGCTCATTTGGAAGCTCAGTTGGCTTCTTCACTGGCTTTGAAGTCCCCTTCTGAATATCGCCAATGTCTCCTATCATACATACGCTTCCTTGCAAGAGAAGCAGATGAGTCCCGTTTGCGAGAAGTCTGTGAGAGTTTTCTTGGACCTCCTACTGGAATGATGGAAAATACATCTTTACATTTGAAGAACTTAGCATGGGATCCTTGTGTCCTTGGAATGAGGAAACATAAACTTTTACGAGAAGAAATTCTTCCAGCAATGGCTTCAAATAGGAAAGTACAACGATTGCTTAATGAATTCATGGATCTCATTTCCGAATATGAAAGTGTTGAAACAAATATAGAGAAAAAATCTCACGCTTCACCAACAGAGAAAGCACCAGCAGCTGAATTAATGGACTCTTCCCCAACGGTGACAAATCAACTGAAGTTGGTACCTGCAACAACAGATCAAATGAAGTCTGTTCATGCTTCAACTGATATAGTGGGCTCCTCCCAGGTAGCAGTAAATCAAGAAAATTCTGCTCCACTAGCTAAAGATAAATTTGATTCAGATCATCCACCACCAGTGATTGATCAAATTAGTCTTACCTCACAAGATGCAGGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1006

Amino Acids

110.65

Weight (kDa)

6.96

Isoelectric Point (pI)

36.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	9 - 330	3.5e-47	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	13 - 221	1.2e-20	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	14 - 159	2.2e-28	WDR5 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	27 - 189	3.2e-08	WDR35/TULP4 N-terminal
Beta-prop_Aladin	PF25460	27 - 190	3.5e-09	Aladin seven-bladed propeller
Beta-prop_THOC3	PF25174	70 - 189	1.7e-19	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	70 - 170	4e-18	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	70 - 157	2e-14	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	70 - 154	3.4e-08	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	70 - 152	1.1e-10	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	71 - 160	5.3e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	71 - 167	4.1e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	74 - 169	1.1e-09	WDR3 second beta-propeller domain
WD40	PF00400	74 - 112	1.7e-10	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	82 - 217	3.5e-15	WDHD1 first WD40 domain
WD40	PF00400	117 - 153	2.2e-06	WD domain, G-beta repeat
Hira	PF07569	649 - 848	8.2e-50	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	230, 795
Acc36I	ACCTGC	3 cut(s)	2326, 2854, 3002
Acc65I	GGTACC	1 cut(s)	2842
AccB1I	GGYRCC	2 cut(s)	1363, 2842
AccIII	TCCGGA	1 cut(s)	2177
AcoI	YGGCCR	4 cut(s)	13, 297, 1510, 2358
AcsI	RAATTY	6 cut(s)	2278, 2296, 2655, 2705, 2926, 2951
AcuI	CTGAAG	1 cut(s)	2855
AcvI	CACGTG	1 cut(s)	2408
AcyI	GRCGYC	2 cut(s)	535, 1321
AdeI	CACNNNGTG	2 cut(s)	554, 1941
AfaI	GTAC	3 cut(s)	2400, 2688, 2844
AfiI	CCNNNNNNNGG	5 cut(s)	22, 173, 531, 976, 2561
AflIII	ACRYGT	1 cut(s)	2405
AgeI	ACCGGT	1 cut(s)	996
AhdI	GACNNNNNGTC	1 cut(s)	495
AjiI	CACGTC	2 cut(s)	857, 1952
AjnI	CCWGG	6 cut(s)	166, 381, 908, 2269, 2360, 2904
AjuI	GAANNNNNNNTTGG	2 cut(s)	2006, 2038
AloI	GAACNNNNNNTCC	2 cut(s)	154, 186
Alw21I	GWGCWC	1 cut(s)	2413
Alw26I	GTCTC	6 cut(s)	282, 1141, 1205, 1942, 2081, 2480
AlwNI	CAGNNNCTG	4 cut(s)	131, 608, 1109, 2795
Aor13HI	TCCGGA	1 cut(s)	2177
AoxI	GGCC	6 cut(s)	13, 297, 1510, 1542, 2230, 2358
ApeKI	GCWGC	6 cut(s)	1103, 1247, 1760, 1982, 2327, 2792
ApoI	RAATTY	6 cut(s)	2278, 2296, 2655, 2705, 2926, 2951
ArsI	GACNNNNNNTTYG	2 cut(s)	1279, 1311
AseI	ATTAAT	1 cut(s)	2801
AsiGI	ACCGGT	1 cut(s)	996
Asp700I	GAANNNNTTC	2 cut(s)	2655, 2658
Asp718I	GGTACC	1 cut(s)	2842
AspA2I	CCTAGG	1 cut(s)	649
AspS9I	GGNCC	5 cut(s)	820, 1295, 1542, 2230, 2552
AsuC2I	CCSGG	2 cut(s)	17, 1691
AsuHPI	GGTGA	8 cut(s)	540, 986, 991, 1055, 2175, 2395, 2763, 2833
AsuII	TTCGAA	1 cut(s)	764
AvaII	GGWCC	3 cut(s)	820, 1295, 2552
AvrII	CCTAGG	1 cut(s)	649
BalI	TGGCCA	2 cut(s)	299, 2360
BamHI	GGATCC	3 cut(s)	403, 2039, 2608
BanI	GGYRCC	2 cut(s)	1363, 2842
BanII	GRGCYC	4 cut(s)	204, 917, 1399, 2900
BbrPI	CACGTG	1 cut(s)	2408
BbsI	GAAGAC	4 cut(s)	467, 773, 1422, 1722
Bbv12I	GWGCWC	1 cut(s)	2413
BbvI	GCAGC	6 cut(s)	1115, 1234, 1747, 1969, 2314, 2804
BccI	CCATC	9 cut(s)	537, 914, 944, 1270, 1322, 1910, 1963, 2131, 2563
BcgI	CGANNNNNNTGC	4 cut(s)	614, 648, 2309, 2343
BciT130I	CCWGG	6 cut(s)	168, 383, 910, 2271, 2362, 2906
BclI	TGATCA	4 cut(s)	67, 136, 1435, 2983
BcnI	CCSGG	2 cut(s)	17, 1691
BcoDI	GTCTC	6 cut(s)	282, 1141, 1205, 1942, 2081, 2480
BfaI	CTAG	5 cut(s)	378, 650, 1016, 1668, 2939
BfmI	CTRYAG	1 cut(s)	252
BfuAI	ACCTGC	3 cut(s)	2326, 2854, 3002
BisI	GCNGC	6 cut(s)	1104, 1248, 1761, 1983, 2328, 2793
BlnI	CCTAGG	1 cut(s)	649
BlpI	GCTNAGC	2 cut(s)	102, 1113
BlsI	GCNGC	6 cut(s)	1105, 1249, 1762, 1984, 2329, 2794
Bme1390I	CCNGG	8 cut(s)	17, 168, 383, 910, 1691, 2271, 2362, 2906
Bme18I	GGWCC	3 cut(s)	820, 1295, 2552
BmeRI	GACNNNNNGTC	1 cut(s)	495
BmgBI	CACGTC	2 cut(s)	857, 1952
BmgT120I	GGNCC	5 cut(s)	820, 1295, 1542, 2230, 2552
BmrFI	CCNGG	8 cut(s)	17, 168, 383, 910, 1691, 2271, 2362, 2906
BmrI	ACTGGG	1 cut(s)	511
BmsI	GCATC	3 cut(s)	997, 2125, 2998
BmuI	ACTGGG	1 cut(s)	511
BoxI	GACNNNNGTC	1 cut(s)	474
BpiI	GAAGAC	4 cut(s)	467, 773, 1422, 1722
BplI	GAGNNNNNCTC	2 cut(s)	466, 498
BpmI	CTGGAG	4 cut(s)	264, 493, 931, 1332
Bpu10I	CCTNAGC	2 cut(s)	720, 2246
Bpu1102I	GCTNAGC	2 cut(s)	102, 1113
Bpu14I	TTCGAA	1 cut(s)	764
BpuEI	CTTGAG	2 cut(s)	635, 1187
BpuMI	CCSGG	2 cut(s)	17, 1691
BsaAI	YACGTR	1 cut(s)	2408
BsaBI	GATNNNNATC	2 cut(s)	2179, 2961
BsaHI	GRCGYC	2 cut(s)	535, 1321
BsaI	GGTCTC	2 cut(s)	282, 1141
BsaJI	CCNNGG	6 cut(s)	593, 649, 1748, 2270, 2620, 2904
BsaWI	WCCGGW	3 cut(s)	822, 996, 2177
Bsc4I	CCNNNNNNNGG	5 cut(s)	22, 173, 531, 976, 2561
Bse118I	RCCGGY	2 cut(s)	996, 2368
Bse1I	ACTGG	6 cut(s)	300, 476, 506, 2445, 2566, 2975
Bse3DI	GCAATG	3 cut(s)	349, 2330, 2673
Bse8I	GATNNNNATC	2 cut(s)	2179, 2961
BseAI	TCCGGA	1 cut(s)	2177
BseBI	CCWGG	6 cut(s)	168, 383, 910, 2271, 2362, 2906
BseDI	CCNNGG	6 cut(s)	593, 649, 1748, 2270, 2620, 2904
BseGI	GGATG	5 cut(s)	548, 955, 1150, 1453, 2965
BseJI	GATNNNNATC	2 cut(s)	2179, 2961
BseLI	CCNNNNNNNGG	5 cut(s)	22, 173, 531, 976, 2561
BseMI	GCAATG	3 cut(s)	349, 2330, 2673
BseMII	CTCAG	8 cut(s)	56, 734, 1200, 1229, 1412, 1671, 2324, 2438
BseNI	ACTGG	6 cut(s)	300, 476, 506, 2445, 2566, 2975
BseRI	GAGGAG	1 cut(s)	2890
BseXI	GCAGC	6 cut(s)	1115, 1234, 1747, 1969, 2314, 2804
BseYI	CCCAGC	2 cut(s)	1081, 1371
BsgI	GTGCAG	1 cut(s)	720
BshFI	GGCC	6 cut(s)	15, 299, 1512, 1544, 2232, 2360
BshNI	GGYRCC	2 cut(s)	1363, 2842
BshTI	ACCGGT	1 cut(s)	996
BsiHKAI	GWGCWC	1 cut(s)	2413
BsiSI	CCGG	6 cut(s)	16, 823, 997, 1690, 2178, 2369
BslFI	GGGAC	3 cut(s)	1387, 2437, 2503
BslI	CCNNNNNNNGG	5 cut(s)	22, 173, 531, 976, 2561
BsmAI	GTCTC	6 cut(s)	282, 1141, 1205, 1942, 2081, 2480
BsmBI	CGTCTC	1 cut(s)	1942
BsmFI	GGGAC	3 cut(s)	1387, 2437, 2503
BsmI	GAATGC	2 cut(s)	598, 2213
BsnI	GGCC	6 cut(s)	15, 299, 1512, 1544, 2232, 2360
Bso31I	GGTCTC	2 cut(s)	282, 1141
Bsp119I	TTCGAA	1 cut(s)	764
Bsp1286I	GDGCHC	5 cut(s)	204, 917, 1399, 2413, 2900
Bsp13I	TCCGGA	1 cut(s)	2177
Bsp1720I	GCTNAGC	2 cut(s)	102, 1113
BspANI	GGCC	6 cut(s)	15, 299, 1512, 1544, 2232, 2360
BspCNI	CTCAG	8 cut(s)	55, 733, 1201, 1228, 1411, 1670, 2323, 2437
BspEI	TCCGGA	1 cut(s)	2177
BspHI	TCATGA	1 cut(s)	154
BspMAI	CTGCAG	1 cut(s)	256
BspMI	ACCTGC	3 cut(s)	2326, 2854, 3002
BspT104I	TTCGAA	1 cut(s)	764
BspT107I	GGYRCC	2 cut(s)	1363, 2842
BspTNI	GGTCTC	2 cut(s)	282, 1141
BsrDI	GCAATG	3 cut(s)	349, 2330, 2673
BsrFI	RCCGGY	2 cut(s)	996, 2368
BsrI	ACTGG	6 cut(s)	300, 476, 506, 2445, 2566, 2975
BssAI	RCCGGY	2 cut(s)	996, 2368
BssECI	CCNNGG	6 cut(s)	593, 649, 1748, 2270, 2620, 2904
BssNI	GRCGYC	2 cut(s)	535, 1321
BssT1I	CCWWGG	4 cut(s)	593, 649, 1748, 2620
Bst2UI	CCWGG	6 cut(s)	168, 383, 910, 2271, 2362, 2906
Bst6I	CTCTTC	3 cut(s)	1022, 2225, 2815
BstACI	GRCGYC	2 cut(s)	535, 1321
BstAPI	GCANNNNNTGC	2 cut(s)	1109, 2135
BstBAI	YACGTR	1 cut(s)	2408
BstBI	TTCGAA	1 cut(s)	764
BstC8I	GCNNGC	7 cut(s)	850, 1062, 1126, 1221, 2140, 2321, 2325
BstEII	GGTNACC	2 cut(s)	546, 992
BstENI	CCTNNNNNAGG	1 cut(s)	529
BstF5I	GGATG	5 cut(s)	548, 955, 1150, 1453, 2965
BstMAI	GTCTC	6 cut(s)	282, 1141, 1205, 1942, 2081, 2480
BstMWI	GCNNNNNNNGC	9 cut(s)	1070, 1109, 1202, 1372, 2135, 2429, 2507, 2792, 2939
BstNI	CCWGG	6 cut(s)	168, 383, 910, 2271, 2362, 2906
BstNSI	RCATGY	1 cut(s)	942
BstPAI	GACNNNNGTC	1 cut(s)	474
BstPI	GGTNACC	2 cut(s)	546, 992
BstSCI	CCNGG	8 cut(s)	15, 166, 381, 908, 1689, 2269, 2360, 2904
BstSFI	CTRYAG	1 cut(s)	252
BstV1I	GCAGC	6 cut(s)	1115, 1234, 1747, 1969, 2314, 2804
BstV2I	GAAGAC	4 cut(s)	467, 773, 1422, 1722
BsuRI	GGCC	6 cut(s)	15, 299, 1512, 1544, 2232, 2360
BtgZI	GCGATG	1 cut(s)	1020
BtrI	CACGTC	2 cut(s)	857, 1952
BtsCI	GGATG	5 cut(s)	548, 955, 1150, 1453, 2965
BtsI	GCAGTG	3 cut(s)	199, 354, 1545
BveI	ACCTGC	3 cut(s)	2326, 2854, 3002
Cac8I	GCNNGC	7 cut(s)	850, 1062, 1126, 1221, 2140, 2321, 2325
CaiI	CAGNNNCTG	4 cut(s)	131, 608, 1109, 2795
CciI	TCATGA	1 cut(s)	154
Cfr10I	RCCGGY	2 cut(s)	996, 2368
Cfr13I	GGNCC	5 cut(s)	820, 1295, 1542, 2230, 2552
CseI	GACGC	2 cut(s)	524, 1329
Csp6I	GTAC	3 cut(s)	2399, 2687, 2843
CspAI	ACCGGT	1 cut(s)	996
CspCI	CAANNNNNGTGG	2 cut(s)	169, 204
CviQI	GTAC	3 cut(s)	2399, 2687, 2843
DraI	TTTAAA	1 cut(s)	1263
DraIII	CACNNNGTG	2 cut(s)	554, 1941
DriI	GACNNNNNGTC	1 cut(s)	495
EaeI	YGGCCR	4 cut(s)	13, 297, 1510, 2358
Eam1104I	CTCTTC	3 cut(s)	1022, 2225, 2815
Eam1105I	GACNNNNNGTC	1 cut(s)	495
EarI	CTCTTC	3 cut(s)	1022, 2225, 2815
Eco130I	CCWWGG	4 cut(s)	593, 649, 1748, 2620
Eco24I	GRGCYC	4 cut(s)	204, 917, 1399, 2900
Eco31I	GGTCTC	2 cut(s)	282, 1141
Eco32I	GATATC	1 cut(s)	1857
Eco47I	GGWCC	3 cut(s)	820, 1295, 2552
Eco57I	CTGAAG	1 cut(s)	2855
Eco72I	CACGTG	1 cut(s)	2408
Eco91I	GGTNACC	2 cut(s)	546, 992
EcoNI	CCTNNNNNAGG	1 cut(s)	529
EcoO65I	GGTNACC	2 cut(s)	546, 992
EcoRI	GAATTC	1 cut(s)	2705
EcoRII	CCWGG	6 cut(s)	166, 381, 908, 2269, 2360, 2904
EcoRV	GATATC	1 cut(s)	1857
EcoT14I	CCWWGG	4 cut(s)	593, 649, 1748, 2620
EcoT22I	ATGCAT	1 cut(s)	2215
EcoT38I	GRGCYC	4 cut(s)	204, 917, 1399, 2900
ErhI	CCWWGG	4 cut(s)	593, 649, 1748, 2620
Esp3I	CGTCTC	1 cut(s)	1942
FalI	AAGNNNNNCTT	4 cut(s)	866, 898, 2643, 2675
FaqI	GGGAC	3 cut(s)	1387, 2437, 2503
FbaI	TGATCA	4 cut(s)	67, 136, 1435, 2983
Fnu4HI	GCNGC	6 cut(s)	1104, 1248, 1761, 1983, 2328, 2793
FokI	GGATG	5 cut(s)	555, 962, 1157, 1460, 2952
FriOI	GRGCYC	4 cut(s)	204, 917, 1399, 2900
Fsp4HI	GCNGC	6 cut(s)	1104, 1248, 1761, 1983, 2328, 2793
FspBI	CTAG	5 cut(s)	378, 650, 1016, 1668, 2939
GluI	GCNGC	6 cut(s)	1104, 1248, 1761, 1983, 2328, 2793
GsaI	CCCAGC	2 cut(s)	1085, 1375
GsuI	CTGGAG	4 cut(s)	264, 493, 931, 1332
HaeIII	GGCC	6 cut(s)	15, 299, 1512, 1544, 2232, 2360
HapII	CCGG	6 cut(s)	16, 823, 997, 1690, 2178, 2369
HgaI	GACGC	2 cut(s)	524, 1329
Hin1I	GRCGYC	2 cut(s)	535, 1321
HincII	GTYRAC	1 cut(s)	85
HindII	GTYRAC	1 cut(s)	85
HindIII	AAGCTT	1 cut(s)	1203
HpaI	GTTAAC	1 cut(s)	85
HpaII	CCGG	6 cut(s)	16, 823, 997, 1690, 2178, 2369
HphI	GGTGA	8 cut(s)	540, 986, 991, 1055, 2175, 2395, 2763, 2833
Hpy166II	GTNNAC	5 cut(s)	85, 711, 750, 1891, 1933
Hpy8I	GTNNAC	5 cut(s)	85, 711, 750, 1891, 1933
HpyAV	CCTTC	7 cut(s)	719, 1185, 1377, 1438, 1579, 1639, 2466
HpyCH4IV	ACGT	5 cut(s)	210, 856, 1623, 1951, 2407
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1070, 1109, 1202, 1372, 2135, 2429, 2507, 2792, 2939
HpySE526I	ACGT	5 cut(s)	210, 856, 1623, 1951, 2407
Hsp92I	GRCGYC	2 cut(s)	535, 1321
Kpn2I	TCCGGA	1 cut(s)	2177
KpnI	GGTACC	1 cut(s)	2846
Ksp22I	TGATCA	4 cut(s)	67, 136, 1435, 2983
KspAI	GTTAAC	1 cut(s)	85
LmnI	GCTCC	6 cut(s)	667, 912, 1255, 2376, 2903, 2938
Lsp1109I	GCAGC	6 cut(s)	1115, 1234, 1747, 1969, 2314, 2804
LweI	GCATC	3 cut(s)	997, 2125, 2998
MaeI	CTAG	5 cut(s)	378, 650, 1016, 1668, 2939
MaeII	ACGT	5 cut(s)	210, 856, 1623, 1951, 2407
MfeI	CAATTG	1 cut(s)	807
MhlI	GDGCHC	5 cut(s)	204, 917, 1399, 2413, 2900
MlsI	TGGCCA	2 cut(s)	299, 2360
MluNI	TGGCCA	2 cut(s)	299, 2360
MlyI	GAGTC	9 cut(s)	29, 281, 484, 823, 1207, 2081, 2197, 2525, 2801
Mox20I	TGGCCA	2 cut(s)	299, 2360
Mph1103I	ATGCAT	1 cut(s)	2215
MroI	TCCGGA	1 cut(s)	2177
MroXI	GAANNNNTTC	2 cut(s)	2655, 2658
MscI	TGGCCA	2 cut(s)	299, 2360
MslI	CAYNNNNRTG	1 cut(s)	1622
Msp20I	TGGCCA	2 cut(s)	299, 2360
MspA1I	CMGCKG	1 cut(s)	2795
MspI	CCGG	6 cut(s)	16, 823, 997, 1690, 2178, 2369
MspR9I	CCNGG	8 cut(s)	17, 168, 383, 910, 1691, 2271, 2362, 2906
MunI	CAATTG	1 cut(s)	807
Mva1269I	GAATGC	2 cut(s)	598, 2213
MvaI	CCWGG	6 cut(s)	168, 383, 910, 2271, 2362, 2906
MwoI	GCNNNNNNNGC	9 cut(s)	1070, 1109, 1202, 1372, 2135, 2429, 2507, 2792, 2939
NciI	CCSGG	2 cut(s)	17, 1691
NmuCI	GTSAC	9 cut(s)	371, 497, 546, 557, 992, 1867, 2026, 2383, 2821
NsiI	ATGCAT	1 cut(s)	2215
NspI	RCATGY	1 cut(s)	942
NspV	TTCGAA	1 cut(s)	764
PagI	TCATGA	1 cut(s)	154
PctI	GAATGC	2 cut(s)	598, 2213
PdmI	GAANNNNTTC	2 cut(s)	2655, 2658
PfeI	GAWTC	3 cut(s)	145, 582, 2957
PfoI	TCCNGGA	1 cut(s)	908
PinAI	ACCGGT	1 cut(s)	996
PkrI	GCNGC	6 cut(s)	1105, 1249, 1762, 1984, 2329, 2794
PleI	GAGTC	9 cut(s)	29, 281, 483, 822, 1207, 2081, 2197, 2524, 2801
PmaCI	CACGTG	1 cut(s)	2408
PmlI	CACGTG	1 cut(s)	2408
PpsI	GAGTC	9 cut(s)	29, 281, 483, 822, 1207, 2081, 2197, 2524, 2801
Ppu21I	YACGTR	1 cut(s)	2408
PshAI	GACNNNNGTC	1 cut(s)	474
PshBI	ATTAAT	1 cut(s)	2801
PsiI	TTATAA	2 cut(s)	230, 795
Psp6I	CCWGG	6 cut(s)	166, 381, 908, 2269, 2360, 2904
PspCI	CACGTG	1 cut(s)	2408
PspEI	GGTNACC	2 cut(s)	546, 992
PspFI	CCCAGC	2 cut(s)	1081, 1371
PspGI	CCWGG	6 cut(s)	166, 381, 908, 2269, 2360, 2904
PspPI	GGNCC	5 cut(s)	820, 1295, 1542, 2230, 2552
PstI	CTGCAG	1 cut(s)	256
PstNI	CAGNNNCTG	4 cut(s)	131, 608, 1109, 2795
PvuII	CAGCTG	1 cut(s)	2795
RsaI	GTAC	3 cut(s)	2400, 2688, 2844
RsaNI	GTAC	3 cut(s)	2399, 2687, 2843
RseI	CAYNNNNRTG	1 cut(s)	1622
SatI	GCNGC	6 cut(s)	1104, 1248, 1761, 1983, 2328, 2793
Sau96I	GGNCC	5 cut(s)	820, 1295, 1542, 2230, 2552
SchI	GAGTC	9 cut(s)	29, 281, 484, 823, 1207, 2081, 2197, 2525, 2801
ScrFI	CCNGG	8 cut(s)	17, 168, 383, 910, 1691, 2271, 2362, 2906
SduI	GDGCHC	5 cut(s)	204, 917, 1399, 2413, 2900
SfaNI	GCATC	3 cut(s)	997, 2125, 2998
SfcI	CTRYAG	1 cut(s)	252
SfuI	TTCGAA	1 cut(s)	764
SinI	GGWCC	3 cut(s)	820, 1295, 2552
SmiMI	CAYNNNNRTG	1 cut(s)	1622
SmlI	CTYRAG	2 cut(s)	614, 1166
SmoI	CTYRAG	2 cut(s)	614, 1166
SspI	AATATT	2 cut(s)	1989, 2354
SspMI	CTAG	5 cut(s)	378, 650, 1016, 1668, 2939
StyD4I	CCNGG	8 cut(s)	15, 166, 381, 908, 1689, 2269, 2360, 2904
StyI	CCWWGG	4 cut(s)	593, 649, 1748, 2620
TaiI	ACGT	5 cut(s)	213, 859, 1626, 1954, 2410
TaqI	TCGA	6 cut(s)	290, 764, 1274, 1698, 1744, 2223
TatI	WGTACW	2 cut(s)	2398, 2686
TfiI	GAWTC	3 cut(s)	145, 582, 2957
TseFI	GTSAC	9 cut(s)	371, 497, 546, 557, 992, 1867, 2026, 2383, 2821
TseI	GCWGC	6 cut(s)	1103, 1247, 1760, 1982, 2327, 2792
Tsp45I	GTSAC	9 cut(s)	371, 497, 546, 557, 992, 1867, 2026, 2383, 2821
TspGWI	ACGGA	3 cut(s)	53, 2083, 2409
VpaK11BI	GGWCC	3 cut(s)	820, 1295, 2552
VspI	ATTAAT	1 cut(s)	2801
XagI	CCTNNNNNAGG	1 cut(s)	529
XapI	RAATTY	6 cut(s)	2278, 2296, 2655, 2705, 2926, 2951
XceI	RCATGY	1 cut(s)	942
XmaJI	CCTAGG	1 cut(s)	649
XmnI	GAANNNNTTC	2 cut(s)	2655, 2658
XspI	CTAG	5 cut(s)	378, 650, 1016, 1668, 2939
Zsp2I	ATGCAT	1 cut(s)	2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.