Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

63842567 .. 63848709

Gene Structure 6143 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030587

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGATTGCAGAAAAGCCCAGTTGGATTAGGCATGAGGGTTTGCAAATTTTCTCCATTGATGTTCAGCCTGGTGGGCTTAGGGTTGCCACTGGTGGGGGTGACCACAAGGTGCGGGTATGGAACATGAAATCCCTGGGCAGGGATTTCTCAAATGAAGAATCAGCCCAAAGGCTACTTGCAACCCTCCGTGATCACTTCGGTTCTGTGAATTGTGTTAGGTGGGCTAAGCATGGTCGCTATCTTGCATCAGGATCTGATGATCAAGTAATTCTAATTCACGAGAGGAAGCCAGGTTCAGGAACCACTGAGTTTGGCAGTGGAGAGCCCCCTGATGTCGAGAATTGGAAAGTTGCAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTCAATTGGTCTCCAGATGACTCGATGCTGGCCAGTGGAAGTTTAGATAACACTATACACATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGCCATTCTAGCTTGGTTAAAGGAGTTACTTGGGATCCGATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACTGTAATTATTTGGCGAACAAGTGACTGGAGTCTTGCTCACAAAACAGACGGCCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGACGGCTTGGATGGTCCCCTTGTGGTCATTTCATTACTACCACTCATGGGTTCCAGAAACCAAGGCATTCAGCACCTGTTCTAGAGAGAGGGGAATGGTCTGCTACTTTTGACTTCTTAGGACATAATGCACCTGTGATTGTGGTGAAGTTTAACCACTCAATGTTTAAGAGGAATTTTTCCAATGCTCAAGAAGGGAAAGTTGCACCTGTTGGGTGGACCAATGGCACCTCAAAAATAGGTGGGAAAGAAAAAGAACTACAACCTTATAATGTTATAGCAATCGGCAGTCAGGATCGTACAATAACTGTATGGACGACTGCAAGTCCCCGCCCTCTCTTTGTAGCAAAGCATTTCTTCACTCAAAGTGTTGTGGATTTATCTTGGAGCCCCGATGGTTATTCACTTTTCGCATGTTCTTTGGACGGTTCAGTGGCTACTTTCCATTTTGAGGTGAAAGAGCTTGGCCACCGGTTAAGTGATGCTGAACTAGATGAATTAAAGAGAAGTCGTTATGGTGATGTTAGAGGTAGGCAGGTAAATTTAGCAGAAAGCCCAGCACAGTTATTGCTTGAGGCAGCTTCTGCTAAGCAAGCCCCCAAAAAAAAAGTGGTTCGGGAAGTTCAGCAAAGCCAGACAGTCAAACCTTCTGCGGATGTGGTGGTTGCAACAAAAGCATCTGATGGTCAAGTTGATGATAGGAAGAAGAGTGGAGGAGCTTCTGGTGATGTTTTAAATAAAGTTTCAATGCCTGCCCGCATTTCTAGTCCTGTAAAGCAAAGAGAATATAGACGACCTGATGGTCGAAAGAGGATTATTCCGGAGGCAGTTGGCGTGCCTCTCCAGAAGGAAAATAATTCTGTTGGGGCTCAGTCCCAGGCACTTGACTTCCCTCTAATGCCTTCTGATCACAGAAAGGATGATAGTGGGTTAGTTGCTGCTGATAGGGAGATTAAAGAAAATTCTGTCAGGGAAGCTCTTATGAGAGGCAATGATATAAAGGACGGACAGGGGGTCACCGCTAGAGCTATGATTACCAACAGCCTTGTTATTGAGAAGGTTCCCACCTCCACAGGTAGAGATGAAAGCATAAATATAGAGCAGTCAGGAACTGTGAAGGCTTCTAATTCAATATGTGGTTCTAGTCCTATTCTTTCAATTAGGGTGTTTGATAAGAAGGAAGCGGAAGATGCTGTACCATATTGCTTTGAAGCTCGGCCTAAGGAACATGCTGCAAATGACATTATTAACGTGGGAAATTCACTGATCATGAAAGAAACAGAAATCACCTGCTCAAAAGGGTTGCAAACACTCTGGTCTGATAGAATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGCAAACTTTTGGGCTGTTGGATGTGAAGATGGATGCATCCAGCGGAACTGTCTCCTTCATGACTCGCTGTCATCTCTGGTTGCTTTAAACCCAAATCCATCTGCAAACGATGCAGGCATGATCAAAGTTATATCAGCAAAGCTATCAAGATCTGGATCTCCTCTTGTTGTTCTCGCTACTCGCCATGCCTTCCTTTTTGACATGGGACTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTTCTGGGTCAAATTTTGCAAGCTCCTGGCATTTGGGTTTGACGCAGAGCGGTGAGCTGGCTGGTTTGCAGGTTGATGTTAAGAAATATTTGGCTAGGAAGCCAGGTTGGAGCAGGGTGACTGACGATGGAGTACAGACACGTGCTCATTTGGAAGCTCAGCTGGCTTCTTTGCTGGCGTTAAAGTCCCCTAATGAATATCGCCAATGCCTTCTATCATATATACGCTTCCTTGCTAGAGAAGCAGATGAGTCTCGTCTACGAGAAGTCTGTGAGAGTTTCCTTGGACCTCCTACTGGAATGGTGGAAGCTTCTTTAGATTCAAAGAACTTGGCATGGGATCCTTTTGTGCTTGGAATGAGGAAACATAAGCTCTTACGAGAAGATATTCTCCCGGCAATGGCTTCAAATCGAAAAGTACAGCGTTTGCTCAATGAATTCATGGATCTAATTTCTGAATATGAAAGTGCTGAAATAAATGTGGAGTCAAAAACTCAAACTTCAACAGCACGTCTGTCGGCAGCTGATCCAATGGAGTCTGCTCCATCCAGTAAAAATAAAATGGATTCTGTCCCTTTGGCAACTGATCAAGCAAAGCCTGTTCCCGATCCAACTGATCAGAAAGACTCCACTCAGTTAGCAACAGCTAGAGAAAATTCTGCTTCAACAGCGCATGATAAAGTGAATTCAGATCCACCAAAGCTCAATCACGTTACTCATGCCCCACAAAATGATGCAGGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1000

Amino Acids

109.37

Weight (kDa)

7.63

Isoelectric Point (pI)

36.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 373	5e-55	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	13 - 91	6.7e-06	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	14 - 98	9.7e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	15 - 200	6.2e-32	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 201	1.8e-16	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	22 - 193	1.3e-11	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	55 - 262	8.7e-21	CDC20/Fizzy WD40 domain
WD40	PF00400	57 - 92	1.5e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	66 - 271	1.4e-26	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	7.3e-08	WDR35/TULP4 N-terminal
WD40_Prp19	PF24814	111 - 199	3.6e-14	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 195	8.2e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	112 - 210	2.7e-17	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	112 - 208	4.3e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 210	1.6e-09	WDR3 second beta-propeller domain
WD40	PF00400	116 - 153	5.4e-10	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	121 - 258	3e-15	WDHD1 first WD40 domain
Beta-prop_Aladin	PF25460	122 - 233	1.2e-08	Aladin seven-bladed propeller
WD40	PF00400	158 - 194	2.6e-06	WD domain, G-beta repeat
Hira	PF07569	685 - 844	3.5e-31	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	924
AarI	CACCTGC	1 cut(s)	1952
AasI	GACNNNNNNGTC	1 cut(s)	1455
Acc36I	ACCTGC	4 cut(s)	1180, 1952, 2314, 2981
AccB1I	GGYRCC	1 cut(s)	881
AccBSI	CCGCTC	1 cut(s)	2304
AccI	GTMKAC	1 cut(s)	2512
AccIII	TCCGGA	1 cut(s)	1474
AciI	CCGC	8 cut(s)	112, 985, 1307, 1411, 1674, 1838, 2056, 2304
AcoI	YGGCCR	3 cut(s)	420, 619, 1120
AcsI	RAATTY	9 cut(s)	45, 829, 1195, 1615, 1912, 2266, 2690, 2908, 2938
AcvI	CACGTG	1 cut(s)	2396
AdeI	CACNNNGTG	1 cut(s)	109
AfaI	GTAC	4 cut(s)	955, 1851, 2388, 2673
AfiI	CCNNNNNNNGG	8 cut(s)	93, 138, 139, 296, 702, 1105, 2549, 2653
AflIII	ACRYGT	1 cut(s)	2393
AgeI	ACCGGT	1 cut(s)	1125
AgsI	TTSAA	8 cut(s)	1401, 1785, 1812, 1865, 2577, 2661, 2757, 2919
AhdI	GACNNNNNGTC	3 cut(s)	978, 1667, 2080
AjiI	CACGTC	1 cut(s)	2765
AjnI	CCWGG	6 cut(s)	67, 132, 289, 1530, 2279, 2356
AloI	GAACNNNNNNTCC	4 cut(s)	113, 145, 277, 309
Alw21I	GWGCWC	1 cut(s)	2401
Alw26I	GTCTC	3 cut(s)	405, 2069, 2511
AlwNI	CAGNNNCTG	4 cut(s)	254, 731, 1238, 1766
Aor13HI	TCCGGA	1 cut(s)	1474
AoxI	GGCC	5 cut(s)	420, 493, 619, 1120, 1871
ApeKI	GCWGC	4 cut(s)	1232, 1592, 1886, 2774
ApoI	RAATTY	9 cut(s)	45, 829, 1195, 1615, 1912, 2266, 2690, 2908, 2938
AsiGI	ACCGGT	1 cut(s)	1125
Asp700I	GAANNNNTTC	1 cut(s)	2640
AspLEI	GCGC	1 cut(s)	2926
AspS9I	GGNCC	4 cut(s)	493, 669, 873, 2540
AsuC2I	CCSGG	1 cut(s)	2648
AsuHPI	GGTGA	9 cut(s)	110, 811, 1120, 1184, 1391, 1663, 1933, 2318, 2383
AvaII	GGWCC	3 cut(s)	669, 873, 2540
AxyI	CCTNAGG	1 cut(s)	1875
BalI	TGGCCA	2 cut(s)	422, 1122
BamHI	GGATCC	2 cut(s)	526, 2593
BanI	GGYRCC	1 cut(s)	881
BanII	GRGCYC	3 cut(s)	327, 1046, 1525
BarI	GAAGNNNNNNTAC	2 cut(s)	1833, 1865
BauI	CACGAG	1 cut(s)	278
BbrPI	CACGTG	1 cut(s)	2396
Bbv12I	GWGCWC	1 cut(s)	2401
BbvI	GCAGC	4 cut(s)	1244, 1579, 1873, 2786
BccI	CCATC	8 cut(s)	660, 1043, 1331, 1448, 2036, 2119, 2375, 2806
BceAI	ACGGC	2 cut(s)	634, 674
BcgI	CGANNNNNNTGC	2 cut(s)	2751, 2785
BciT130I	CCWGG	6 cut(s)	69, 134, 291, 1532, 2281, 2358
BclI	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2133, 2839, 2869
BcnI	CCSGG	1 cut(s)	2648
BcoDI	GTCTC	3 cut(s)	405, 2069, 2511
BfaI	CTAG	9 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2349, 2490, 2901
BfmI	CTRYAG	1 cut(s)	375
BfuAI	ACCTGC	4 cut(s)	1180, 1952, 2314, 2981
BglI	GCCNNNNNGGC	1 cut(s)	73
BglII	AGATCT	1 cut(s)	2162
BisI	GCNGC	4 cut(s)	1233, 1593, 1887, 2775
BlpI	GCTNAGC	3 cut(s)	225, 1242, 2412
BlsI	GCNGC	4 cut(s)	1234, 1594, 1888, 2776
Bme1390I	CCNGG	7 cut(s)	69, 134, 291, 1532, 2281, 2358, 2648
Bme18I	GGWCC	3 cut(s)	669, 873, 2540
BmeRI	GACNNNNNGTC	3 cut(s)	978, 1667, 2080
BmgBI	CACGTC	1 cut(s)	2765
BmgT120I	GGNCC	4 cut(s)	493, 669, 873, 2540
BmrFI	CCNGG	7 cut(s)	69, 134, 291, 1532, 2281, 2358, 2648
BmrI	ACTGGG	2 cut(s)	12, 634
BmsI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2113, 2977
BmuI	ACTGGG	2 cut(s)	12, 634
BoxI	GACNNNNGTC	1 cut(s)	597
BplI	GAGNNNNNCTC	2 cut(s)	589, 621
BpmI	CTGGAG	3 cut(s)	387, 616, 1481
Bpu10I	CCTNAGC	2 cut(s)	77, 2234
Bpu1102I	GCTNAGC	3 cut(s)	225, 1242, 2412
BpuEI	CTTGAG	2 cut(s)	828, 1247
BpuMI	CCSGG	1 cut(s)	2648
BsaAI	YACGTR	1 cut(s)	2396
BsaBI	GATNNNNATC	1 cut(s)	2167
BsaI	GGTCTC	1 cut(s)	405
BsaJI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2536
BsaWI	WCCGGW	2 cut(s)	1125, 1474
Bsc4I	CCNNNNNNNGG	8 cut(s)	93, 138, 139, 296, 702, 1105, 2549, 2653
Bse118I	RCCGGY	1 cut(s)	1125
Bse1I	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2554, 2802
Bse21I	CCTNAGG	1 cut(s)	1875
Bse3DI	GCAATG	4 cut(s)	360, 472, 1651, 2658
Bse8I	GATNNNNATC	1 cut(s)	2167
BseAI	TCCGGA	1 cut(s)	1474
BseBI	CCWGG	6 cut(s)	69, 134, 291, 1532, 2281, 2358
BseDI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2536
BseGI	GGATG	7 cut(s)	671, 1315, 1579, 2039, 2049, 2051, 2798
BseJI	GATNNNNATC	1 cut(s)	2167
BseLI	CCNNNNNNNGG	8 cut(s)	93, 138, 139, 296, 702, 1105, 2549, 2653
BseMI	GCAATG	4 cut(s)	360, 472, 1651, 2658
BseMII	CTCAG	4 cut(s)	297, 1538, 2426, 2900
BseNI	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2554, 2802
BseRI	GAGGAG	2 cut(s)	1383, 2163
BseXI	GCAGC	4 cut(s)	1244, 1579, 1873, 2786
BseYI	CCCAGC	1 cut(s)	1210
BshFI	GGCC	5 cut(s)	422, 495, 621, 1122, 1873
BshNI	GGYRCC	1 cut(s)	881
BshTI	ACCGGT	1 cut(s)	1125
BsiHKAI	GWGCWC	1 cut(s)	2401
BsiSI	CCGG	3 cut(s)	1126, 1475, 2648
BslFI	GGGAC	6 cut(s)	655, 966, 1513, 2232, 2425, 2810
BslI	CCNNNNNNNGG	8 cut(s)	93, 138, 139, 296, 702, 1105, 2549, 2653
BsmAI	GTCTC	3 cut(s)	405, 2069, 2511
BsmFI	GGGAC	6 cut(s)	655, 966, 1513, 2232, 2425, 2810
BsmI	GAATGC	1 cut(s)	721
BsnI	GGCC	5 cut(s)	422, 495, 621, 1122, 1873
Bso31I	GGTCTC	1 cut(s)	405
Bsp1286I	GDGCHC	4 cut(s)	327, 1046, 1525, 2401
Bsp13I	TCCGGA	1 cut(s)	1474
Bsp1720I	GCTNAGC	3 cut(s)	225, 1242, 2412
BspACI	CCGC	8 cut(s)	112, 985, 1307, 1411, 1674, 1838, 2056, 2304
BspANI	GGCC	5 cut(s)	422, 495, 621, 1122, 1873
BspCNI	CTCAG	4 cut(s)	298, 1537, 2425, 2899
BspEI	TCCGGA	1 cut(s)	1474
BspHI	TCATGA	2 cut(s)	1923, 2071
BspMAI	CTGCAG	1 cut(s)	379
BspMI	ACCTGC	4 cut(s)	1180, 1952, 2314, 2981
BspT107I	GGYRCC	1 cut(s)	881
BspTNI	GGTCTC	1 cut(s)	405
BsrBI	CCGCTC	1 cut(s)	2304
BsrDI	GCAATG	4 cut(s)	360, 472, 1651, 2658
BsrFI	RCCGGY	1 cut(s)	1125
BsrI	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2554, 2802
BssAI	RCCGGY	1 cut(s)	1125
BssECI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2536
BssSI	CACGAG	1 cut(s)	278
BssT1I	CCWWGG	2 cut(s)	716, 2536
Bst2BI	CACGAG	1 cut(s)	278
Bst2UI	CCWGG	6 cut(s)	69, 134, 291, 1532, 2281, 2358
Bst4CI	ACNGT	8 cut(s)	571, 964, 1082, 1218, 1294, 1768, 1999, 2063
Bst6I	CTCTTC	1 cut(s)	1355
BstAPI	GCANNNNNTGC	2 cut(s)	1238, 2123
BstBAI	YACGTR	1 cut(s)	2396
BstEII	GGTNACC	2 cut(s)	98, 1669
BstF5I	GGATG	7 cut(s)	671, 1315, 1579, 2039, 2049, 2051, 2798
BstHHI	GCGC	1 cut(s)	2926
BstMAI	GTCTC	3 cut(s)	405, 2069, 2511
BstNI	CCWGG	6 cut(s)	69, 134, 291, 1532, 2281, 2358
BstNSI	RCATGY	2 cut(s)	1071, 1886
BstPAI	GACNNNNGTC	1 cut(s)	597
BstPI	GGTNACC	2 cut(s)	98, 1669
BstSCI	CCNGG	7 cut(s)	67, 132, 289, 1530, 2279, 2356, 2646
BstSFI	CTRYAG	1 cut(s)	375
BstV1I	GCAGC	4 cut(s)	1244, 1579, 1873, 2786
BstX2I	RGATCY	9 cut(s)	251, 388, 526, 641, 2162, 2168, 2593, 2698, 2944
BstYI	RGATCY	9 cut(s)	251, 388, 526, 641, 2162, 2168, 2593, 2698, 2944
Bsu36I	CCTNAGG	1 cut(s)	1875
BsuRI	GGCC	5 cut(s)	422, 495, 621, 1122, 1873
BtrI	CACGTC	1 cut(s)	2765
BtsCI	GGATG	7 cut(s)	671, 1315, 1579, 2039, 2049, 2051, 2798
BtsI	GCAGTG	2 cut(s)	322, 477
BtsIMutI	CAGTG	9 cut(s)	87, 303, 322, 430, 477, 622, 1092, 1916, 1995
BveI	ACCTGC	4 cut(s)	1180, 1952, 2314, 2981
CaiI	CAGNNNCTG	4 cut(s)	254, 731, 1238, 1766
CciI	TCATGA	2 cut(s)	1923, 2071
CfoI	GCGC	1 cut(s)	2926
Cfr10I	RCCGGY	1 cut(s)	1125
Cfr13I	GGNCC	4 cut(s)	493, 669, 873, 2540
CseI	GACGC	1 cut(s)	2305
Csp6I	GTAC	4 cut(s)	954, 1850, 2387, 2672
CspAI	ACCGGT	1 cut(s)	1125
CspCI	CAANNNNNGTGG	2 cut(s)	292, 327
CviQI	GTAC	4 cut(s)	954, 1850, 2387, 2672
DraI	TTTAAA	2 cut(s)	1389, 2100
DraIII	CACNNNGTG	1 cut(s)	109
DrdI	GACNNNNNNGTC	1 cut(s)	1455
DriI	GACNNNNNGTC	3 cut(s)	978, 1667, 2080
DseDI	GACNNNNNNGTC	1 cut(s)	1455
EaeI	YGGCCR	3 cut(s)	420, 619, 1120
Eam1104I	CTCTTC	1 cut(s)	1355
Eam1105I	GACNNNNNGTC	3 cut(s)	978, 1667, 2080
EarI	CTCTTC	1 cut(s)	1355
Eco130I	CCWWGG	2 cut(s)	716, 2536
Eco24I	GRGCYC	3 cut(s)	327, 1046, 1525
Eco31I	GGTCTC	1 cut(s)	405
Eco47I	GGWCC	3 cut(s)	669, 873, 2540
Eco72I	CACGTG	1 cut(s)	2396
Eco81I	CCTNAGG	1 cut(s)	1875
Eco91I	GGTNACC	2 cut(s)	98, 1669
EcoO65I	GGTNACC	2 cut(s)	98, 1669
EcoRI	GAATTC	2 cut(s)	2690, 2938
EcoRII	CCWGG	6 cut(s)	67, 132, 289, 1530, 2279, 2356
EcoT14I	CCWWGG	2 cut(s)	716, 2536
EcoT22I	ATGCAT	1 cut(s)	2051
EcoT38I	GRGCYC	3 cut(s)	327, 1046, 1525
ErhI	CCWWGG	2 cut(s)	716, 2536
FalI	AAGNNNNNCTT	2 cut(s)	995, 1027
FaqI	GGGAC	6 cut(s)	655, 966, 1513, 2232, 2425, 2810
FauI	CCCGC	3 cut(s)	105, 992, 1418
FbaI	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2133, 2839, 2869
FblI	GTMKAC	1 cut(s)	2512
Fnu4HI	GCNGC	4 cut(s)	1233, 1593, 1887, 2775
FokI	GGATG	7 cut(s)	678, 1322, 1586, 2036, 2046, 2058, 2785
FriOI	GRGCYC	3 cut(s)	327, 1046, 1525
Fsp4HI	GCNGC	4 cut(s)	1233, 1593, 1887, 2775
FspBI	CTAG	9 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2349, 2490, 2901
GlaI	GCGC	1 cut(s)	2925
GluI	GCNGC	4 cut(s)	1233, 1593, 1887, 2775
GsaI	CCCAGC	1 cut(s)	1214
GsuI	CTGGAG	3 cut(s)	387, 616, 1481
HaeIII	GGCC	5 cut(s)	422, 495, 621, 1122, 1873
HapII	CCGG	3 cut(s)	1126, 1475, 2648
HgaI	GACGC	1 cut(s)	2305
HhaI	GCGC	1 cut(s)	2926
Hin6I	GCGC	1 cut(s)	2924
HinP1I	GCGC	1 cut(s)	2924
HindIII	AAGCTT	1 cut(s)	2562
HpaII	CCGG	3 cut(s)	1126, 1475, 2648
HphI	GGTGA	9 cut(s)	110, 811, 1120, 1184, 1391, 1663, 1933, 2318, 2383
Hpy166II	GTNNAC	2 cut(s)	873, 2513
Hpy8I	GTNNAC	2 cut(s)	873, 2513
HpyCH4III	ACNGT	8 cut(s)	571, 964, 1082, 1218, 1294, 1768, 1999, 2063
HpyCH4IV	ACGT	4 cut(s)	1905, 2395, 2764, 2964
HpySE526I	ACGT	4 cut(s)	1905, 2395, 2764, 2964
HspAI	GCGC	1 cut(s)	2924
Kpn2I	TCCGGA	1 cut(s)	1474
Ksp22I	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2133, 2839, 2869
LmnI	GCTCC	6 cut(s)	542, 1041, 1370, 2282, 2364, 2800
Lsp1109I	GCAGC	4 cut(s)	1244, 1579, 1873, 2786
LweI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2113, 2977
MaeI	CTAG	9 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2349, 2490, 2901
MaeII	ACGT	4 cut(s)	1905, 2395, 2764, 2964
MaeIII	GTNAC	7 cut(s)	98, 517, 590, 1669, 1993, 2371, 2965
MbiI	CCGCTC	1 cut(s)	2304
MboII	GAAGA	7 cut(s)	167, 1003, 1369, 1372, 1853, 2051, 2648
MfeI	CAATTG	1 cut(s)	394
MflI	RGATCY	9 cut(s)	251, 388, 526, 641, 2162, 2168, 2593, 2698, 2944
MhlI	GDGCHC	4 cut(s)	327, 1046, 1525, 2401
MlsI	TGGCCA	2 cut(s)	422, 1122
MluNI	TGGCCA	2 cut(s)	422, 1122
MlyI	GAGTC	7 cut(s)	404, 607, 2069, 2513, 2747, 2798, 2873
MmeI	TCCRAC	3 cut(s)	2011, 2342, 2888
Mox20I	TGGCCA	2 cut(s)	422, 1122
Mph1103I	ATGCAT	1 cut(s)	2051
MroI	TCCGGA	1 cut(s)	1474
MroXI	GAANNNNTTC	1 cut(s)	2640
MscI	TGGCCA	2 cut(s)	422, 1122
Msp20I	TGGCCA	2 cut(s)	422, 1122
MspA1I	CMGCKG	3 cut(s)	2056, 2416, 2777
MspI	CCGG	3 cut(s)	1126, 1475, 2648
MspR9I	CCNGG	7 cut(s)	69, 134, 291, 1532, 2281, 2358, 2648
MunI	CAATTG	1 cut(s)	394
Mva1269I	GAATGC	1 cut(s)	721
MvaI	CCWGG	6 cut(s)	69, 134, 291, 1532, 2281, 2358
NciI	CCSGG	1 cut(s)	2648
NmeAIII	GCCGAG	1 cut(s)	1849
NmuCI	GTSAC	5 cut(s)	98, 590, 1669, 1993, 2371
NsiI	ATGCAT	1 cut(s)	2051
NspI	RCATGY	2 cut(s)	1071, 1886
PagI	TCATGA	2 cut(s)	1923, 2071
PaqCI	CACCTGC	1 cut(s)	1952
PasI	CCCWGGG	1 cut(s)	133
PctI	GAATGC	1 cut(s)	721
PdmI	GAANNNNTTC	1 cut(s)	2640
PfeI	GAWTC	3 cut(s)	158, 2573, 2819
PinAI	ACCGGT	1 cut(s)	1125
PkrI	GCNGC	4 cut(s)	1234, 1594, 1888, 2776
PleI	GAGTC	7 cut(s)	404, 606, 2069, 2512, 2746, 2797, 2873
PmaCI	CACGTG	1 cut(s)	2396
PmlI	CACGTG	1 cut(s)	2396
PpsI	GAGTC	7 cut(s)	404, 606, 2069, 2512, 2746, 2797, 2873
Ppu21I	YACGTR	1 cut(s)	2396
PshAI	GACNNNNGTC	1 cut(s)	597
PsiI	TTATAA	1 cut(s)	924
Psp6I	CCWGG	6 cut(s)	67, 132, 289, 1530, 2279, 2356
PspCI	CACGTG	1 cut(s)	2396
PspEI	GGTNACC	2 cut(s)	98, 1669
PspFI	CCCAGC	1 cut(s)	1210
PspGI	CCWGG	6 cut(s)	67, 132, 289, 1530, 2279, 2356
PspPI	GGNCC	4 cut(s)	493, 669, 873, 2540
PstI	CTGCAG	1 cut(s)	379
PstNI	CAGNNNCTG	4 cut(s)	254, 731, 1238, 1766
PsuI	RGATCY	9 cut(s)	251, 388, 526, 641, 2162, 2168, 2593, 2698, 2944
PvuII	CAGCTG	2 cut(s)	2416, 2777
RsaI	GTAC	4 cut(s)	955, 1851, 2388, 2673
RsaNI	GTAC	4 cut(s)	954, 1850, 2387, 2672
SatI	GCNGC	4 cut(s)	1233, 1593, 1887, 2775
Sau96I	GGNCC	4 cut(s)	493, 669, 873, 2540
SchI	GAGTC	7 cut(s)	404, 607, 2069, 2513, 2747, 2798, 2873
ScrFI	CCNGG	7 cut(s)	69, 134, 291, 1532, 2281, 2358, 2648
SduI	GDGCHC	4 cut(s)	327, 1046, 1525, 2401
SfaNI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2113, 2977
SfcI	CTRYAG	1 cut(s)	375
SinI	GGWCC	3 cut(s)	669, 873, 2540
SmlI	CTYRAG	2 cut(s)	843, 1226
SmoI	CTYRAG	2 cut(s)	843, 1226
SsiI	CCGC	8 cut(s)	112, 985, 1307, 1411, 1674, 1838, 2056, 2304
SspI	AATATT	1 cut(s)	2342
SspMI	CTAG	9 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2349, 2490, 2901
StyD4I	CCNGG	7 cut(s)	67, 132, 289, 1530, 2279, 2356, 2646
StyI	CCWWGG	2 cut(s)	716, 2536
TaaI	ACNGT	8 cut(s)	571, 964, 1082, 1218, 1294, 1768, 1999, 2063
TaiI	ACGT	4 cut(s)	1908, 2398, 2767, 2967
TaqI	TCGA	4 cut(s)	336, 413, 1459, 2665
TatI	WGTACW	2 cut(s)	2386, 2671
TfiI	GAWTC	3 cut(s)	158, 2573, 2819
TscAI	CASTG	9 cut(s)	94, 310, 322, 430, 484, 629, 1092, 1923, 2002
TseFI	GTSAC	5 cut(s)	98, 590, 1669, 1993, 2371
TseI	GCWGC	4 cut(s)	1232, 1592, 1886, 2774
Tsp45I	GTSAC	5 cut(s)	98, 590, 1669, 1993, 2371
TspGWI	ACGGA	2 cut(s)	176, 1674
TspRI	CASTG	9 cut(s)	94, 310, 322, 430, 484, 629, 1092, 1923, 2002
VpaK11BI	GGWCC	3 cut(s)	669, 873, 2540
XapI	RAATTY	9 cut(s)	45, 829, 1195, 1615, 1912, 2266, 2690, 2908, 2938
XbaI	TCTAGA	1 cut(s)	736
XceI	RCATGY	2 cut(s)	1071, 1886
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1261
XmiI	GTMKAC	1 cut(s)	2512
XmnI	GAANNNNTTC	1 cut(s)	2640
XspI	CTAG	9 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2349, 2490, 2901
Zsp2I	ATGCAT	1 cut(s)	2051

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.