Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

63704780 .. 63711590

Gene Structure 6811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00330210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGATTGCAGAGAAGCCCAGTTGGATTAGGCACGAGGGTTTGCAAATTTTCTCCATTGATGTTCAGCCTGGTGGACTCAGGGTTGCCACTGGTGGGGGTGACCACAAGGTGCGGGTATGGAACATGAAATCCCTGGGCAGGGATTTCTCAAATGAAGAATCAGCCCAGAGGCTACTTGCAACCCTCCGTGATCACTTTGGTTCTGTGAATTGTGTTAGGTGGGCTAAGCATGGTCGGTATCTTGCATCAGGATCTGATGATCAAGTAATTCTACTTCATGAGAGGAAGCCAGGTTCAGGAACCACTGAGTTTGGCAGTGGAGAGCCTCCTGATGTCGAGAATTGGAAAGTTGCAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTCAATTGGTCTCCAGATGACTCGATGCTGGCCAGTGGAAGTTTAGATAACACTATACACATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGTCATTCTAGCTTGGTTAAAGGAGTTACTTGGGATCCGATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACTGTAATTATTTGGCGAACAAGTGACTGGAGTCTTGCTCACAAAACAGATGGCCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGGCGGCTTGGATGGTCCCCTTGTGGTCATTTCATTACTACCACTCATGGGTTCCAGAAACCAAGGCATTCAGCACCTGTTCTAGAGAGAGGGGAATGGTCTGCTACTTTTGACTTCTTAGGACATAATGCACCTGTGATTGTGGTGAAGTTTAACCACTCAATGTTTAAGAGGAATTTTTCCAATGCTCAAGAAGGGAAAGTTGCACCTGTTGGGTGGACCAATGGCACTTCAAAAATAGGTGGGAAAGAAAAAGAATTACAACCTTATAATGTTATAGCAATCGGGAGTCAGGATCGTACAATAACTGTATGGACTACTGCAAGTCCCCGCCCTCTCTTTGTAGCAAAGCATTTCTTCACTCAAAGTGTTGTGGATTTATCTTGGAGCCCCGATGGTTATTCACTTTTCGCATGTTCTTTGGACGGTTCAGTGGCTACTTTCCATTTTGAGGTGAAAGAGCTTGGTCACCGGTTAAGTGATGCTGAGCTAGATGAATTAAAGAGAAGTCGTTATGGCGATGTTAGAGGTAGGCAGGTAAATTTAGCTGAAAGCCCAGCACAGTTATTGCTTGAGGCAGCTTCTGCTAAGCAAGCCCCAAAGAAAAAAGTGGTTCGGGATGTTCAGCAAAGCCAGACAGTCAAACCTTCTGCGGATGTGGTGGTTGCAACAAAAGCATCTGATGGTCACGTTGATGATAGGAAGAAGAGTGGAGGAGCTTCTGGTGATGTTTTAAATAAAGTTTCAATGCCTGCCCACATTTCTAGTCCTGTAAAGCAAAGAGAATATAGACGACCTGATGGTCGAAAGAGGATTATTCCAGAGGCAGTTGGTGTGCCTCTCCAGAAGGAAAATAATTCTGTTGGGGCTCAGTCCCAGGCACTTGACTTCCCTCTAATGCCTTCTGATCACAGAAAGGATGATAGTGGGTTAGTTGCTGATGATAGGGGGATTAAAGAAAATTCTGTCAGGGAAACACTTATGAGAGGCAATGATATAAAGGACGGACAGGGGGTCACCGCTAGAGCTATGATTACCAACAGCCTTGTTATTGAGAAGGTTCCCACCTCCACAGGTAGAGATGAAAGCATAAATATAGAACAGTCAGGAACTGTGAAGGCTTCTAATTCAATATGTGGTTCTAGTCCTATTCTTTCAATTAGGGTGTTTGATAAGAAGGAAGCGGAAGATGCTGTACCATATTGCTTGGAAGCTCGGCCTAAGGAACATGCTGCAAATGACATTATTAACGTGGGAAATTCACTGATCATGAAAGAAACCGAAATCACCTGCACAAAAGGGTTGCAAACACTTTGGTCTGATAGAATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGCAAACTTTTGGGCTGTTGGATGTGAAGATGGATGCATCCAGGTTTACACAAAGTGTGGGAGACGCGCTATGCCGACTATGATGGTAGGATCTGCAGCAGTTTTTATAGATTGTGACGAGTGCTGGAAGTTGTTTTTGGTTACCAGAAAAGGATCCTTGTATCTATGGGATCTATTAAAGCGGAACTGTCTCCTTCATGACTCGTTGTCATCTCTGGTTGCTTTAAACCCAAATCCATCTGCAAATGATGCAGGCATGATCAAAGTTATATCAGCAAAGCTATCAAGATCTGGATCTCCTCTTGTTGTCCTCGCTACTCGCCATGCCTTCCTTTTTGACATGGGACTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTTCTGGGTCAAATTTTGCAAGCTCCTGGCATTTGGGTTTGACGCAGAGCGGTGAGCTGGCTGGTTTGCAGGTTGATGTTAAGAAATATTTGGCTAGGAAGCCAGGTTGGAGCAGGGTGACTGACGATGGAGTACAGACACGTGCTCATTTGGAAGCTCAGCTGGCTTCTTTGCTGGCTTTAAAGTCCCCTAATGAATATCGCCAATGCCTTCTATCATATATACGCTTCCTTGCCAGAGAAGCAGATGAGTCTCGTCTACGAGAAGTCTGTGAGAGTTTCCTTGGACCTCCTACTGGAATGGTGGAAGCTACTTCTTTAGATTCAAAGAACTTGGCATGGGATCCTTTTGTGCTTGGAATGAGGAAACATAAGCTCTTACGAGAAGATATTCTCCCGGCAATGGCTTCAAATCGAAAAGTACAGCGTTTGCTCAATGAATTCATGGATCTAATTTCTGAATATGAAAGTGCTGAAATAAATGTAGAGTCAAAAACTCAAACTTCACCAACAGCACGTCTGTCAGCAGCTGATCCAATGGAGTCTGCTCCATCCAGTAAAAATAAAATGGATTCTGTCCCTGTGGCAACTGATCAAGCAAAGCCTGTTCCCGATCCAACTGATCAAAAAGACTCCACTCAGTTAGCAACAGATAGAGAAAATTCTGCTTCAACAGCAAACAATGAAGTTAATTCAGATCCACCAAAGCTCAATCACGTTACTCATGCCCCACAAAATGATGCAGGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1047

Amino Acids

114.83

Weight (kDa)

7.6

Isoelectric Point (pI)

38.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 373	1e-54	CAF1B/HIR1 beta-propeller domain
WD40_Prp19	PF24814	13 - 91	6.8e-06	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	14 - 96	1.6e-07	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	15 - 201	1.1e-31	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 201	4.3e-16	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	21 - 193	8.9e-12	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	54 - 262	1e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	57 - 91	2.1e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	66 - 271	5e-26	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	1e-07	WDR35/TULP4 N-terminal
WD40_Prp19	PF24814	111 - 199	3.8e-14	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 195	8.3e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	112 - 210	2.9e-17	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	112 - 208	4.6e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 210	1.8e-09	WDR3 second beta-propeller domain
WD40	PF00400	116 - 153	5.7e-10	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	121 - 258	3.1e-15	WDHD1 first WD40 domain
Beta-prop_Aladin	PF25460	122 - 233	1.3e-08	Aladin seven-bladed propeller
WD40	PF00400	158 - 194	2.7e-06	WD domain, G-beta repeat
Hira	PF07569	691 - 890	1.2e-47	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	924
AarI	CACCTGC	1 cut(s)	1952
AasI	GACNNNNNNGTC	1 cut(s)	1455
Acc36I	ACCTGC	4 cut(s)	1180, 1952, 2452, 3125
AccBSI	CCGCTC	1 cut(s)	2442
AccI	GTMKAC	1 cut(s)	2650
AccII	CGCG	1 cut(s)	2079
AciI	CCGC	8 cut(s)	112, 658, 985, 1307, 1674, 1838, 2194, 2442
AcoI	YGGCCR	2 cut(s)	420, 619
AcsI	RAATTY	8 cut(s)	45, 829, 1195, 1615, 1912, 2404, 2831, 3052
AcvI	CACGTG	1 cut(s)	2534
AdeI	CACNNNGTG	2 cut(s)	109, 2067
AfaI	GTAC	4 cut(s)	955, 1851, 2526, 2814
AfiI	CCNNNNNNNGG	9 cut(s)	93, 138, 139, 296, 654, 702, 1105, 2687, 2794
AflIII	ACRYGT	1 cut(s)	2531
AgeI	ACCGGT	1 cut(s)	1125
AgsI	TTSAA	7 cut(s)	888, 1401, 1785, 1812, 2718, 2802, 3063
AhdI	GACNNNNNGTC	2 cut(s)	1667, 2218
AjiI	CACGTC	1 cut(s)	2909
AjnI	CCWGG	7 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494
AjuI	GAANNNNNNNTTGG	2 cut(s)	2132, 2164
AloI	GAACNNNNNNTCC	4 cut(s)	113, 145, 277, 309
Alw21I	GWGCWC	1 cut(s)	2539
Alw26I	GTCTC	4 cut(s)	405, 2068, 2207, 2649
AlwNI	CAGNNNCTG	5 cut(s)	254, 731, 1238, 1766, 2921
AoxI	GGCC	3 cut(s)	420, 619, 1871
ApeKI	GCWGC	4 cut(s)	1232, 1886, 2108, 2918
ApoI	RAATTY	8 cut(s)	45, 829, 1195, 1615, 1912, 2404, 2831, 3052
AsiGI	ACCGGT	1 cut(s)	1125
Asp700I	GAANNNNTTC	1 cut(s)	2781
AspLEI	GCGC	1 cut(s)	2081
AspS9I	GGNCC	3 cut(s)	669, 873, 2678
AsuC2I	CCSGG	1 cut(s)	2789
AvaII	GGWCC	3 cut(s)	669, 873, 2678
AxyI	CCTNAGG	1 cut(s)	1875
BalI	TGGCCA	2 cut(s)	422, 621
BamHI	GGATCC	3 cut(s)	526, 2165, 2734
BanII	GRGCYC	2 cut(s)	1046, 1525
BarI	GAAGNNNNNNTAC	4 cut(s)	258, 290, 1833, 1865
BauI	CACGAG	1 cut(s)	32
BbrPI	CACGTG	1 cut(s)	2534
Bbv12I	GWGCWC	1 cut(s)	2539
BbvI	GCAGC	4 cut(s)	1244, 1873, 2120, 2930
BciT130I	CCWGG	7 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496
BclI	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2271, 2983, 3013
BcnI	CCSGG	1 cut(s)	2789
BcoDI	GTCTC	4 cut(s)	405, 2068, 2207, 2649
BfaI	CTAG	7 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2487
BfmI	CTRYAG	2 cut(s)	375, 2106
BfuAI	ACCTGC	4 cut(s)	1180, 1952, 2452, 3125
BglII	AGATCT	1 cut(s)	2300
BisI	GCNGC	5 cut(s)	659, 1233, 1887, 2109, 2919
BlpI	GCTNAGC	4 cut(s)	225, 1140, 1242, 2550
BlsI	GCNGC	5 cut(s)	660, 1234, 1888, 2110, 2920
Bme1390I	CCNGG	8 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496, 2789
Bme18I	GGWCC	3 cut(s)	669, 873, 2678
BmeRI	GACNNNNNGTC	2 cut(s)	1667, 2218
BmgBI	CACGTC	1 cut(s)	2909
BmgT120I	GGNCC	3 cut(s)	669, 873, 2678
BmiI	GGNNCC	9 cut(s)	301, 528, 538, 671, 707, 1043, 1716, 2167, 2736
BmrFI	CCNGG	8 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496, 2789
BmrI	ACTGGG	2 cut(s)	12, 634
BmsI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
BmuI	ACTGGG	2 cut(s)	12, 634
BoxI	GACNNNNGTC	1 cut(s)	597
BplI	GAGNNNNNCTC	2 cut(s)	589, 621
BpmI	CTGGAG	3 cut(s)	387, 616, 1481
Bpu10I	CCTNAGC	1 cut(s)	2372
Bpu1102I	GCTNAGC	4 cut(s)	225, 1140, 1242, 2550
BpuEI	CTTGAG	2 cut(s)	828, 1247
BpuMI	CCSGG	1 cut(s)	2789
BsaAI	YACGTR	1 cut(s)	2534
BsaBI	GATNNNNATC	1 cut(s)	2305
BsaI	GGTCTC	1 cut(s)	405
BsaJI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2674
BsaWI	WCCGGW	1 cut(s)	1125
Bsc4I	CCNNNNNNNGG	9 cut(s)	93, 138, 139, 296, 654, 702, 1105, 2687, 2794
Bse118I	RCCGGY	1 cut(s)	1125
Bse1I	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2692, 2946
Bse21I	CCTNAGG	1 cut(s)	1875
Bse3DI	GCAATG	4 cut(s)	360, 472, 1651, 2799
Bse8I	GATNNNNATC	1 cut(s)	2305
BseBI	CCWGG	7 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496
BseDI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2674
BseGI	GGATG	8 cut(s)	671, 1279, 1315, 1579, 2039, 2049, 2051, 2942
BseJI	GATNNNNATC	1 cut(s)	2305
BseLI	CCNNNNNNNGG	9 cut(s)	93, 138, 139, 296, 654, 702, 1105, 2687, 2794
BseMI	GCAATG	4 cut(s)	360, 472, 1651, 2799
BseMII	CTCAG	6 cut(s)	91, 297, 1131, 1538, 2564, 3044
BseNI	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2692, 2946
BseRI	GAGGAG	2 cut(s)	1383, 2301
BseXI	GCAGC	4 cut(s)	1244, 1873, 2120, 2930
BseYI	CCCAGC	1 cut(s)	1210
BsgI	GTGCAG	1 cut(s)	1930
Bsh1236I	CGCG	1 cut(s)	2079
BshFI	GGCC	3 cut(s)	422, 621, 1873
BshTI	ACCGGT	1 cut(s)	1125
BsiHKAI	GWGCWC	1 cut(s)	2539
BsiSI	CCGG	2 cut(s)	1126, 2789
BslFI	GGGAC	6 cut(s)	655, 966, 1513, 2370, 2563, 2954
BslI	CCNNNNNNNGG	9 cut(s)	93, 138, 139, 296, 654, 702, 1105, 2687, 2794
BsmAI	GTCTC	4 cut(s)	405, 2068, 2207, 2649
BsmBI	CGTCTC	1 cut(s)	2068
BsmFI	GGGAC	6 cut(s)	655, 966, 1513, 2370, 2563, 2954
BsmI	GAATGC	1 cut(s)	721
BsnI	GGCC	3 cut(s)	422, 621, 1873
Bso31I	GGTCTC	1 cut(s)	405
Bsp1286I	GDGCHC	3 cut(s)	1046, 1525, 2539
Bsp1720I	GCTNAGC	4 cut(s)	225, 1140, 1242, 2550
BspACI	CCGC	8 cut(s)	112, 658, 985, 1307, 1674, 1838, 2194, 2442
BspANI	GGCC	3 cut(s)	422, 621, 1873
BspCNI	CTCAG	6 cut(s)	90, 298, 1132, 1537, 2563, 3043
BspFNI	CGCG	1 cut(s)	2079
BspHI	TCATGA	3 cut(s)	277, 1923, 2209
BspLI	GGNNCC	9 cut(s)	301, 528, 538, 671, 707, 1043, 1716, 2167, 2736
BspMAI	CTGCAG	2 cut(s)	379, 2110
BspMI	ACCTGC	4 cut(s)	1180, 1952, 2452, 3125
BspTNI	GGTCTC	1 cut(s)	405
BsrBI	CCGCTC	1 cut(s)	2442
BsrDI	GCAATG	4 cut(s)	360, 472, 1651, 2799
BsrFI	RCCGGY	1 cut(s)	1125
BsrI	ACTGG	7 cut(s)	18, 94, 423, 599, 629, 2692, 2946
BssAI	RCCGGY	1 cut(s)	1125
BssECI	CCNNGG	5 cut(s)	132, 133, 716, 1530, 2674
BssSI	CACGAG	1 cut(s)	32
BssT1I	CCWWGG	2 cut(s)	716, 2674
Bst2BI	CACGAG	1 cut(s)	32
Bst2UI	CCWGG	7 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496
Bst4CI	ACNGT	9 cut(s)	571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
Bst6I	CTCTTC	1 cut(s)	1355
BstAPI	GCANNNNNTGC	2 cut(s)	1238, 2261
BstBAI	YACGTR	1 cut(s)	2534
BstC8I	GCNNGC	8 cut(s)	420, 1248, 1407, 2266, 2413, 2451, 2556, 2568
BstEII	GGTNACC	4 cut(s)	98, 1121, 1669, 2152
BstENI	CCTNNNNNAGG	1 cut(s)	652
BstF5I	GGATG	8 cut(s)	671, 1279, 1315, 1579, 2039, 2049, 2051, 2942
BstFNI	CGCG	1 cut(s)	2079
BstHHI	GCGC	1 cut(s)	2081
BstMAI	GTCTC	4 cut(s)	405, 2068, 2207, 2649
BstMWI	GCNNNNNNNGC	9 cut(s)	1238, 1247, 1328, 1844, 2012, 2261, 2555, 2633, 3065
BstNI	CCWGG	7 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496
BstNSI	RCATGY	2 cut(s)	1071, 1886
BstPAI	GACNNNNGTC	1 cut(s)	597
BstPI	GGTNACC	4 cut(s)	98, 1121, 1669, 2152
BstSCI	CCNGG	8 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494, 2787
BstSFI	CTRYAG	2 cut(s)	375, 2106
BstUI	CGCG	1 cut(s)	2079
BstV1I	GCAGC	4 cut(s)	1244, 1873, 2120, 2930
Bsu36I	CCTNAGG	1 cut(s)	1875
BsuRI	GGCC	3 cut(s)	422, 621, 1873
BtgZI	GCGATG	1 cut(s)	1188
BtrI	CACGTC	1 cut(s)	2909
BtsCI	GGATG	8 cut(s)	671, 1279, 1315, 1579, 2039, 2049, 2051, 2942
BtsI	GCAGTG	2 cut(s)	322, 477
BtsIMutI	CAGTG	9 cut(s)	87, 303, 322, 430, 477, 622, 1092, 1916, 1995
BveI	ACCTGC	4 cut(s)	1180, 1952, 2452, 3125
Cac8I	GCNNGC	8 cut(s)	420, 1248, 1407, 2266, 2413, 2451, 2556, 2568
CaiI	CAGNNNCTG	5 cut(s)	254, 731, 1238, 1766, 2921
CciI	TCATGA	3 cut(s)	277, 1923, 2209
CfoI	GCGC	1 cut(s)	2081
Cfr10I	RCCGGY	1 cut(s)	1125
Cfr13I	GGNCC	3 cut(s)	669, 873, 2678
CseI	GACGC	2 cut(s)	2085, 2443
Csp6I	GTAC	4 cut(s)	954, 1850, 2525, 2813
CspAI	ACCGGT	1 cut(s)	1125
CspCI	CAANNNNNGTGG	2 cut(s)	292, 327
CviQI	GTAC	4 cut(s)	954, 1850, 2525, 2813
DraI	TTTAAA	3 cut(s)	1389, 2238, 2574
DraIII	CACNNNGTG	2 cut(s)	109, 2067
DrdI	GACNNNNNNGTC	1 cut(s)	1455
DriI	GACNNNNNGTC	2 cut(s)	1667, 2218
DseDI	GACNNNNNNGTC	1 cut(s)	1455
EaeI	YGGCCR	2 cut(s)	420, 619
Eam1104I	CTCTTC	1 cut(s)	1355
Eam1105I	GACNNNNNGTC	2 cut(s)	1667, 2218
EarI	CTCTTC	1 cut(s)	1355
Eco130I	CCWWGG	2 cut(s)	716, 2674
Eco24I	GRGCYC	2 cut(s)	1046, 1525
Eco31I	GGTCTC	1 cut(s)	405
Eco47I	GGWCC	3 cut(s)	669, 873, 2678
Eco72I	CACGTG	1 cut(s)	2534
Eco81I	CCTNAGG	1 cut(s)	1875
Eco91I	GGTNACC	4 cut(s)	98, 1121, 1669, 2152
EcoNI	CCTNNNNNAGG	1 cut(s)	652
EcoO65I	GGTNACC	4 cut(s)	98, 1121, 1669, 2152
EcoRI	GAATTC	1 cut(s)	2831
EcoRII	CCWGG	7 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494
EcoT14I	CCWWGG	2 cut(s)	716, 2674
EcoT22I	ATGCAT	1 cut(s)	2051
EcoT38I	GRGCYC	2 cut(s)	1046, 1525
ErhI	CCWWGG	2 cut(s)	716, 2674
Esp3I	CGTCTC	1 cut(s)	2068
FalI	AAGNNNNNCTT	2 cut(s)	995, 1027
FaqI	GGGAC	6 cut(s)	655, 966, 1513, 2370, 2563, 2954
FauI	CCCGC	2 cut(s)	105, 992
FbaI	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2271, 2983, 3013
FblI	GTMKAC	1 cut(s)	2650
Fnu4HI	GCNGC	5 cut(s)	659, 1233, 1887, 2109, 2919
FokI	GGATG	8 cut(s)	678, 1286, 1322, 1586, 2036, 2046, 2058, 2929
FriOI	GRGCYC	2 cut(s)	1046, 1525
Fsp4HI	GCNGC	5 cut(s)	659, 1233, 1887, 2109, 2919
FspBI	CTAG	7 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2487
GlaI	GCGC	1 cut(s)	2080
GluI	GCNGC	5 cut(s)	659, 1233, 1887, 2109, 2919
GsaI	CCCAGC	1 cut(s)	1214
GsuI	CTGGAG	3 cut(s)	387, 616, 1481
HaeIII	GGCC	3 cut(s)	422, 621, 1873
HapII	CCGG	2 cut(s)	1126, 2789
HgaI	GACGC	2 cut(s)	2085, 2443
HhaI	GCGC	1 cut(s)	2081
Hin6I	GCGC	1 cut(s)	2079
HinP1I	GCGC	1 cut(s)	2079
HpaII	CCGG	2 cut(s)	1126, 2789
Hpy166II	GTNNAC	4 cut(s)	74, 873, 2059, 2651
Hpy188I	TCNGA	8 cut(s)	256, 531, 559, 1336, 1561, 1975, 2851, 3088
Hpy8I	GTNNAC	4 cut(s)	74, 873, 2059, 2651
HpyCH4III	ACNGT	9 cut(s)	571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
HpyCH4IV	ACGT	5 cut(s)	1344, 1905, 2533, 2908, 3108
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1238, 1247, 1328, 1844, 2012, 2261, 2555, 2633, 3065
HpySE526I	ACGT	5 cut(s)	1344, 1905, 2533, 2908, 3108
HspAI	GCGC	1 cut(s)	2079
Ksp22I	TGATCA	7 cut(s)	190, 259, 1561, 1920, 2271, 2983, 3013
LmnI	GCTCC	6 cut(s)	542, 1041, 1370, 2420, 2502, 2944
Lsp1109I	GCAGC	4 cut(s)	1244, 1873, 2120, 2930
LweI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
MaeI	CTAG	7 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2487
MaeII	ACGT	5 cut(s)	1344, 1905, 2533, 2908, 3108
MbiI	CCGCTC	1 cut(s)	2442
MboII	GAAGA	7 cut(s)	167, 1003, 1369, 1372, 1853, 2051, 2789
MfeI	CAATTG	1 cut(s)	394
MhlI	GDGCHC	3 cut(s)	1046, 1525, 2539
MlsI	TGGCCA	2 cut(s)	422, 621
MluNI	TGGCCA	2 cut(s)	422, 621
MlyI	GAGTC	9 cut(s)	69, 404, 607, 952, 2207, 2651, 2888, 2942, 3017
MmeI	TCCRAC	3 cut(s)	2011, 2480, 3032
Mox20I	TGGCCA	2 cut(s)	422, 621
Mph1103I	ATGCAT	1 cut(s)	2051
MroXI	GAANNNNTTC	1 cut(s)	2781
MscI	TGGCCA	2 cut(s)	422, 621
MslI	CAYNNNNRTG	1 cut(s)	1347
Msp20I	TGGCCA	2 cut(s)	422, 621
MspA1I	CMGCKG	2 cut(s)	2554, 2921
MspI	CCGG	2 cut(s)	1126, 2789
MspR9I	CCNGG	8 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496, 2789
MunI	CAATTG	1 cut(s)	394
Mva1269I	GAATGC	1 cut(s)	721
MvaI	CCWGG	7 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496
MvnI	CGCG	1 cut(s)	2079
MwoI	GCNNNNNNNGC	9 cut(s)	1238, 1247, 1328, 1844, 2012, 2261, 2555, 2633, 3065
NciI	CCSGG	1 cut(s)	2789
NlaIV	GGNNCC	9 cut(s)	301, 528, 538, 671, 707, 1043, 1716, 2167, 2736
NmeAIII	GCCGAG	1 cut(s)	1849
NmuCI	GTSAC	8 cut(s)	98, 590, 1121, 1340, 1669, 1993, 2126, 2509
NsiI	ATGCAT	1 cut(s)	2051
NspI	RCATGY	2 cut(s)	1071, 1886
PagI	TCATGA	3 cut(s)	277, 1923, 2209
PaqCI	CACCTGC	1 cut(s)	1952
PasI	CCCWGGG	1 cut(s)	133
PctI	GAATGC	1 cut(s)	721
PdmI	GAANNNNTTC	1 cut(s)	2781
PfeI	GAWTC	3 cut(s)	158, 2714, 2963
PinAI	ACCGGT	1 cut(s)	1125
PkrI	GCNGC	5 cut(s)	660, 1234, 1888, 2110, 2920
PleI	GAGTC	9 cut(s)	69, 404, 606, 951, 2207, 2650, 2887, 2941, 3017
PmaCI	CACGTG	1 cut(s)	2534
PmlI	CACGTG	1 cut(s)	2534
PpsI	GAGTC	9 cut(s)	69, 404, 606, 951, 2207, 2650, 2887, 2941, 3017
Ppu21I	YACGTR	1 cut(s)	2534
PshAI	GACNNNNGTC	1 cut(s)	597
PsiI	TTATAA	1 cut(s)	924
Psp6I	CCWGG	7 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494
PspCI	CACGTG	1 cut(s)	2534
PspEI	GGTNACC	4 cut(s)	98, 1121, 1669, 2152
PspFI	CCCAGC	1 cut(s)	1210
PspGI	CCWGG	7 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494
PspN4I	GGNNCC	9 cut(s)	301, 528, 538, 671, 707, 1043, 1716, 2167, 2736
PspPI	GGNCC	3 cut(s)	669, 873, 2678
PstI	CTGCAG	2 cut(s)	379, 2110
PstNI	CAGNNNCTG	5 cut(s)	254, 731, 1238, 1766, 2921
PvuII	CAGCTG	2 cut(s)	2554, 2921
RsaI	GTAC	4 cut(s)	955, 1851, 2526, 2814
RsaNI	GTAC	4 cut(s)	954, 1850, 2525, 2813
RseI	CAYNNNNRTG	1 cut(s)	1347
SatI	GCNGC	5 cut(s)	659, 1233, 1887, 2109, 2919
Sau96I	GGNCC	3 cut(s)	669, 873, 2678
SchI	GAGTC	9 cut(s)	69, 404, 607, 952, 2207, 2651, 2888, 2942, 3017
ScrFI	CCNGG	8 cut(s)	69, 134, 291, 1532, 2054, 2419, 2496, 2789
SduI	GDGCHC	3 cut(s)	1046, 1525, 2539
SfaNI	GCATC	9 cut(s)	254, 405, 1126, 1340, 1834, 2036, 2058, 2251, 3121
SfcI	CTRYAG	2 cut(s)	375, 2106
SinI	GGWCC	3 cut(s)	669, 873, 2678
SmiMI	CAYNNNNRTG	1 cut(s)	1347
SmlI	CTYRAG	2 cut(s)	843, 1226
SmoI	CTYRAG	2 cut(s)	843, 1226
SsiI	CCGC	8 cut(s)	112, 658, 985, 1307, 1674, 1838, 2194, 2442
SspI	AATATT	1 cut(s)	2480
SspMI	CTAG	7 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2487
StyD4I	CCNGG	8 cut(s)	67, 132, 289, 1530, 2052, 2417, 2494, 2787
StyI	CCWWGG	2 cut(s)	716, 2674
TaaI	ACNGT	9 cut(s)	571, 964, 1082, 1218, 1294, 1758, 1768, 1999, 2201
TaiI	ACGT	5 cut(s)	1347, 1908, 2536, 2911, 3111
TaqI	TCGA	4 cut(s)	336, 413, 1459, 2806
TatI	WGTACW	2 cut(s)	2524, 2812
TauI	GCSGC	1 cut(s)	661
TfiI	GAWTC	3 cut(s)	158, 2714, 2963
TscAI	CASTG	9 cut(s)	94, 310, 322, 430, 484, 629, 1092, 1923, 2002
TseFI	GTSAC	8 cut(s)	98, 590, 1121, 1340, 1669, 1993, 2126, 2509
TseI	GCWGC	4 cut(s)	1232, 1886, 2108, 2918
Tsp45I	GTSAC	8 cut(s)	98, 590, 1121, 1340, 1669, 1993, 2126, 2509
TspGWI	ACGGA	2 cut(s)	176, 1674
TspRI	CASTG	9 cut(s)	94, 310, 322, 430, 484, 629, 1092, 1923, 2002
VpaK11BI	GGWCC	3 cut(s)	669, 873, 2678
XagI	CCTNNNNNAGG	1 cut(s)	652
XapI	RAATTY	8 cut(s)	45, 829, 1195, 1615, 1912, 2404, 2831, 3052
XbaI	TCTAGA	1 cut(s)	736
XceI	RCATGY	2 cut(s)	1071, 1886
XmiI	GTMKAC	1 cut(s)	2650
XmnI	GAANNNNTTC	1 cut(s)	2781
XspI	CTAG	7 cut(s)	501, 737, 1145, 1419, 1677, 1797, 2487
Zsp2I	ATGCAT	1 cut(s)	2051

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.