Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Reverse (-)

39962179 .. 39972517

Gene Structure 10339 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG312200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGATTGCGGAGAAGCCCAGTTGGATTAAGCACGAAGGACTGCAGATATTCTCCATTGATGTTCAGCCCGGTGGGCTTAGGCTTGCCACCGGTGGGGGCGATCACAAGGTACGAATATGGAACATGAAGTCTCTTGGCCGGGATATGGAAAATGAGGACTCAACTCAGAGGCTACTTGCAACCCTCCGTGATCACTTTGGCTCTGTTAACTGTGTTAGGTGGGCTAAGCACAGTCGTTATGTTGCAACAGGGTCTGATGATCAAGTGATTCTAATTCATGAGAGGAAGCCTGGTTCGGGAACCACCGAGTTTGGCAGTGGAGAGCCACCTGATGTTGAGAATTGGAAAGTTATAATGACTTTGAGAGGGCATACTGCTGATGTGGTGGATCTTAATTGGTCTCCAGATGACTCGATGTTGGCCAGTGGAAGTTTAGATAATACTATACATATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGTCACTCTAGCCTGGTTAAAGGAGTTACTTGGGATCCCATTGGCTCGTTCATAGCAAGTCAATCAGACGATAAGACAGTAATTATTTGGAAGACAAGCGACTGGAGTCTTGCTCACAGGACTGACGGTCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGGCGTCTTGGATGGTCACCTTGTGGTCACTTCATTACTACTACTCATGGATTCCAGAAACCAAGGCATTCAGCACCTGTTCTTGAGAGAGGCGAATGGTCTGCCACATTTGACTTCCTAGGACATAATGCTCCTATAATCGTTGTGAAGTTTAACCATTCAATGTTTATGAGGAAGTTTACCAATGCTCAGGAAGGGAAAACTGCACCTGTGGGGTGGACTAATGGGGCTTCGAAGACAGGAGGGAAAGAACCACAACCTTATAATGTTATTGCAATTGGGAGTCAGGACCGGACAATAACTGTATGGACGACTGCAAGCCCACGTCCTCTATTTGTTGCAAAGCATTTCTTTACTCAAAGTGTTGTGGATTTGTCCTGGAGCCCTGATGGTTATTCGCTCTTTGCATGTTCTTTGGATGGTTCGGTGGCTACTTTCCATTTTGAGGTGAAAGAACTTGGTCACCGGTTAAGCGATGCAGAACTAGATGAGTTGAAGAGAAATCGTTATGGTGATGTTAGAGGTCGGCAAGCAAACTTAGCTGAAAGCCCAGCACAGTTATTGCTTGAAGCAGCTTCTGCTAAGCAAGTGGCAAGCAAAAAGGTCTCTCTGGATGTTCAACAAAATCACACACTTGAGAAATCTTCTGCTCATGTGGGGGTTGCAACAAAAGCTTCTGAGACTCAGCTTGCTGATATGAAAAAGAGTGGAGGTGCTGCTCCTGATTGTTTAAATAAAGTTTCGATGGCTACCCGAATATCTGGTCCTGTGAAGCAAAGAGAATATAGACGCCCTGATGGTAGAAAAAGGATTACTCCAGAAGCAGTTGGGGTGCCTTCCCAGCAGGAAAATATCTCTATGGGGGCTCAGTCCCAAGCACTTGACTTCCCTCCCATGTCTTCTGATCATAGGAAGGATGATAATGGTTTAGTTGTTGCTGATAGTGGCATTAGAGAAACTTCTTTCAGGGGAACACTTGGCCGAAGCACTGATACAAAGGAAAGATATGGGGCCACTGCCAGAGCTATGATTACTGAAAGTCTGGTTATTGAGAAGGTTCCTGCTTCCACAAGCAGAGATGAAAGCATAAACGTGGAACAAACAGGAAATGTGAAGGCGTGTAACTCAGTAGGCTCTAGTAGTTCTATTCTTTCAATCCGGGTGTTCGATAAGAAAGAATGGGAAGACACAGTGCCAATTTGTTTGGAAGCTCGACCTTGGGAACAAGCTGCAAATGATGTGTTTGGCATGGGAAATACATTTATTACGAAAGAAACTGAAATCACTTGCACAAGGGGGTTGCAAACTCTTTGGTCTGATAGGATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGTAAACTTTTGGGCTGTTGGCTGTGAAGATGGTTGCATACAGGTTTACACTAAGTGTGGGAGACGTGCTATGCCAACAATGATGGTGGGATCTGCTGCAATATTTATAGATTGTGATGAGTGCTGGAAGTTGTTTTTGGTCACAAAAAAAGGATCCTTTTATCTGTGGGATCTATTCACACGGAACTGTCTTCTTAATGACTCCTTGACATCTCTGGTTACTTCAAACCCAAACCCATCTGCAAAAGATGCAGGCATAATCAAAGTTATATCAGCAAAACTATCAAGATCTGGATCACCTATTGTTGTTCTTGCGACTCGTCATGCATTCCTCTTCGACATGGGCCTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTCCTGGGTCAAATTTTGCAAGTTCTTGGAATTTAACTTCAACACAGAGTGGTGAGCTGGCTGCATTGCAGGTTGATGTTAGGAAATATTTGGCCAGGAAGCCGGTGTGGAGCAGGGTGACTGATGACGGAGTACAGACACGTGCTCATTTGGAAGCTCAGTTGGCTTCTTCACTGGCTTTGAAGTCCCCTTCTGAATATCGCCAATGTCTCCTATCATACATACGCTTCCTTGCAAGGTATTTATATTCATCCCTTGTAGCAAGACCTGTTGTTTCCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

890

Amino Acids

98.02

Weight (kDa)

8.66

Isoelectric Point (pI)

36.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 371	4.3e-55	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	12 - 97	2.8e-06	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	15 - 200	2.3e-33	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	16 - 99	8.6e-09	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 200	1.4e-16	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	22 - 193	1.6e-11	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	54 - 262	1.1e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	57 - 92	9.2e-06	WD domain, G-beta repeat
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	2.7e-08	WDR35/TULP4 N-terminal
Beta-prop_Aladin	PF25460	69 - 231	7.7e-09	Aladin seven-bladed propeller
Beta-prop_WDR3_1st	PF25173	111 - 211	3.1e-18	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	111 - 230	1.6e-19	THOC3 beta-propeller domain
WD40_Prp19	PF24814	111 - 198	1.7e-14	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 195	1.8e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	112 - 208	3.8e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 210	9.6e-10	WDR3 second beta-propeller domain
WD40	PF00400	115 - 153	1.5e-10	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	123 - 258	3.2e-15	WDHD1 first WD40 domain
WD40	PF00400	158 - 194	1.9e-06	WD domain, G-beta repeat
Hira	PF07569	690 - 877	1.3e-43	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	353, 918
Acc36I	ACCTGC	1 cut(s)	2449
AccB1I	GGYRCC	1 cut(s)	1486
AciI	CCGC	1 cut(s)	8
AclWI	GGATC	9 cut(s)	396, 521, 534, 649, 2107, 2157, 2170, 2187, 2311
AcoI	YGGCCR	4 cut(s)	136, 420, 1633, 2481
AcsI	RAATTY	2 cut(s)	2401, 2419
AcvI	CACGTG	1 cut(s)	2531
AcyI	GRCGYC	2 cut(s)	658, 1444
AdeI	CACNNNGTG	3 cut(s)	677, 2064, 2438
AfaI	GTAC	2 cut(s)	111, 2523
AfiI	CCNNNNNNNGG	5 cut(s)	93, 145, 296, 654, 1099
AflIII	ACRYGT	1 cut(s)	2528
AgeI	ACCGGT	2 cut(s)	89, 1119
AgsI	TTSAA	8 cut(s)	816, 1150, 1223, 1274, 1809, 2235, 2430, 2572
AhdI	GACNNNNNGTC	1 cut(s)	618
AjiI	CACGTC	2 cut(s)	980, 2075
AjnI	CCWGG	5 cut(s)	289, 504, 1031, 2392, 2483
AjuI	GAANNNNNNNTTGG	2 cut(s)	2129, 2161
AloI	GAACNNNNNNTCC	2 cut(s)	277, 309
Alw21I	GWGCWC	1 cut(s)	2536
Alw26I	GTCTC	6 cut(s)	135, 405, 1264, 1328, 2065, 2603
AlwI	GGATC	9 cut(s)	396, 521, 534, 649, 2107, 2157, 2170, 2187, 2311
AlwNI	CAGNNNCTG	3 cut(s)	254, 731, 1232
AoxI	GGCC	6 cut(s)	136, 420, 1633, 1665, 2353, 2481
ApeKI	GCWGC	5 cut(s)	1226, 1370, 1883, 2105, 2450
ApoI	RAATTY	2 cut(s)	2401, 2419
ArsI	GACNNNNNNTTYG	2 cut(s)	1402, 1434
AsiGI	ACCGGT	2 cut(s)	89, 1119
AspA2I	CCTAGG	1 cut(s)	772
AspS9I	GGNCC	4 cut(s)	943, 1418, 1665, 2353
AsuC2I	CCSGG	3 cut(s)	69, 140, 1814
AsuHPI	GGTGA	7 cut(s)	663, 1109, 1114, 1178, 2298, 2453, 2518
AsuII	TTCGAA	1 cut(s)	887
AvaII	GGWCC	2 cut(s)	943, 1418
AvrII	CCTAGG	1 cut(s)	772
BalI	TGGCCA	2 cut(s)	422, 2483
BamHI	GGATCC	2 cut(s)	526, 2162
BanI	GGYRCC	1 cut(s)	1486
BanII	GRGCYC	2 cut(s)	1040, 1522
BarI	GAAGNNNNNNTAC	2 cut(s)	1630, 1662
BbrPI	CACGTG	1 cut(s)	2531
BbsI	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2192
Bbv12I	GWGCWC	1 cut(s)	2536
BbvI	GCAGC	5 cut(s)	1238, 1357, 1870, 2092, 2437
BccI	CCATC	8 cut(s)	660, 1037, 1067, 1393, 1445, 2033, 2086, 2254
BcgI	CGANNNNNNTGC	2 cut(s)	737, 771
BciT130I	CCWGG	5 cut(s)	291, 506, 1033, 2394, 2485
BclI	TGATCA	3 cut(s)	190, 259, 1558
BcnI	CCSGG	3 cut(s)	69, 140, 1814
BcoDI	GTCTC	6 cut(s)	135, 405, 1264, 1328, 2065, 2603
BfaI	CTAG	4 cut(s)	501, 773, 1139, 1791
BfmI	CTRYAG	1 cut(s)	41
BfuAI	ACCTGC	1 cut(s)	2449
BglI	GCCNNNNNGGC	1 cut(s)	73
BglII	AGATCT	1 cut(s)	2297
BisI	GCNGC	5 cut(s)	1227, 1371, 1884, 2106, 2451
BlnI	CCTAGG	1 cut(s)	772
BlpI	GCTNAGC	2 cut(s)	225, 1236
BlsI	GCNGC	5 cut(s)	1228, 1372, 1885, 2107, 2452
Bme1390I	CCNGG	8 cut(s)	69, 140, 291, 506, 1033, 1814, 2394, 2485
Bme18I	GGWCC	2 cut(s)	943, 1418
BmeRI	GACNNNNNGTC	1 cut(s)	618
BmgBI	CACGTC	2 cut(s)	980, 2075
BmgT120I	GGNCC	4 cut(s)	943, 1418, 1665, 2353
BmiI	GGNNCC	7 cut(s)	301, 528, 1037, 1488, 1666, 1713, 2164
BmrFI	CCNGG	8 cut(s)	69, 140, 291, 506, 1033, 1814, 2394, 2485
BmrI	ACTGGG	2 cut(s)	12, 634
BmsI	GCATC	2 cut(s)	1120, 2248
BmuI	ACTGGG	2 cut(s)	12, 634
BoxI	GACNNNNGTC	1 cut(s)	597
BpiI	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2192
BplI	GAGNNNNNCTC	2 cut(s)	589, 621
BpmI	CTGGAG	4 cut(s)	387, 616, 1054, 1455
Bpu10I	CCTNAGC	3 cut(s)	77, 843, 2369
Bpu1102I	GCTNAGC	2 cut(s)	225, 1236
Bpu14I	TTCGAA	1 cut(s)	887
BpuEI	CTTGAG	2 cut(s)	758, 1310
BpuMI	CCSGG	3 cut(s)	69, 140, 1814
BsaAI	YACGTR	1 cut(s)	2531
BsaBI	GATNNNNATC	1 cut(s)	2302
BsaHI	GRCGYC	2 cut(s)	658, 1444
BsaI	GGTCTC	2 cut(s)	405, 1264
BsaJI	CCNNGG	4 cut(s)	716, 772, 1871, 2393
BsaWI	WCCGGW	3 cut(s)	89, 945, 1119
Bsc4I	CCNNNNNNNGG	5 cut(s)	93, 145, 296, 654, 1099
Bse118I	RCCGGY	3 cut(s)	89, 1119, 2491
Bse1I	ACTGG	5 cut(s)	18, 423, 599, 629, 2568
Bse3DI	GCAATG	2 cut(s)	472, 2453
Bse8I	GATNNNNATC	1 cut(s)	2302
BseBI	CCWGG	5 cut(s)	291, 506, 1033, 2394, 2485
BseDI	CCNNGG	4 cut(s)	716, 772, 1871, 2393
BseGI	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2639
BseJI	GATNNNNATC	1 cut(s)	2302
BseLI	CCNNNNNNNGG	5 cut(s)	93, 145, 296, 654, 1099
BseMI	GCAATG	2 cut(s)	472, 2453
BseMII	CTCAG	7 cut(s)	179, 857, 1323, 1352, 1535, 1794, 2561
BseNI	ACTGG	5 cut(s)	18, 423, 599, 629, 2568
BseXI	GCAGC	5 cut(s)	1238, 1357, 1870, 2092, 2437
BseYI	CCCAGC	2 cut(s)	1204, 1494
BsgI	GTGCAG	1 cut(s)	843
BshFI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BshNI	GGYRCC	1 cut(s)	1486
BshTI	ACCGGT	2 cut(s)	89, 1119
BsiHKAI	GWGCWC	1 cut(s)	2536
BsiSI	CCGG	7 cut(s)	69, 90, 139, 946, 1120, 1813, 2492
BslFI	GGGAC	2 cut(s)	1510, 2560
BslI	CCNNNNNNNGG	5 cut(s)	93, 145, 296, 654, 1099
BsmAI	GTCTC	6 cut(s)	135, 405, 1264, 1328, 2065, 2603
BsmBI	CGTCTC	1 cut(s)	2065
BsmFI	GGGAC	2 cut(s)	1510, 2560
BsmI	GAATGC	2 cut(s)	721, 2336
BsnI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
Bso31I	GGTCTC	2 cut(s)	405, 1264
Bsp119I	TTCGAA	1 cut(s)	887
Bsp1286I	GDGCHC	3 cut(s)	1040, 1522, 2536
Bsp1720I	GCTNAGC	2 cut(s)	225, 1236
BspACI	CCGC	1 cut(s)	8
BspANI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BspCNI	CTCAG	7 cut(s)	178, 856, 1324, 1351, 1534, 1793, 2560
BspHI	TCATGA	1 cut(s)	277
BspLI	GGNNCC	7 cut(s)	301, 528, 1037, 1488, 1666, 1713, 2164
BspMAI	CTGCAG	1 cut(s)	45
BspMI	ACCTGC	1 cut(s)	2449
BspPI	GGATC	9 cut(s)	396, 521, 534, 649, 2107, 2157, 2170, 2187, 2311
BspT104I	TTCGAA	1 cut(s)	887
BspT107I	GGYRCC	1 cut(s)	1486
BspTNI	GGTCTC	2 cut(s)	405, 1264
BsrDI	GCAATG	2 cut(s)	472, 2453
BsrFI	RCCGGY	3 cut(s)	89, 1119, 2491
BsrI	ACTGG	5 cut(s)	18, 423, 599, 629, 2568
BssAI	RCCGGY	3 cut(s)	89, 1119, 2491
BssECI	CCNNGG	4 cut(s)	716, 772, 1871, 2393
BssNI	GRCGYC	2 cut(s)	658, 1444
BssT1I	CCWWGG	3 cut(s)	716, 772, 1871
Bst2UI	CCWGG	5 cut(s)	291, 506, 1033, 2394, 2485
Bst4CI	ACNGT	9 cut(s)	212, 233, 571, 620, 958, 1212, 1846, 1996, 2198
Bst6I	CTCTTC	2 cut(s)	1145, 2348
BstACI	GRCGYC	2 cut(s)	658, 1444
BstAPI	GCANNNNNTGC	2 cut(s)	1232, 2258
BstBAI	YACGTR	1 cut(s)	2531
BstBI	TTCGAA	1 cut(s)	887
BstC8I	GCNNGC	7 cut(s)	84, 973, 1185, 1249, 1344, 2263, 2448
BstEII	GGTNACC	2 cut(s)	669, 1115
BstENI	CCTNNNNNAGG	1 cut(s)	652
BstF5I	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2639
BstMAI	GTCTC	6 cut(s)	135, 405, 1264, 1328, 2065, 2603
BstMWI	GCNNNNNNNGC	7 cut(s)	73, 1193, 1232, 1325, 1495, 2258, 2552
BstNI	CCWGG	5 cut(s)	291, 506, 1033, 2394, 2485
BstNSI	RCATGY	1 cut(s)	1065
BstPAI	GACNNNNGTC	1 cut(s)	597
BstPI	GGTNACC	2 cut(s)	669, 1115
BstSCI	CCNGG	8 cut(s)	67, 138, 289, 504, 1031, 1812, 2392, 2483
BstSFI	CTRYAG	1 cut(s)	41
BstV1I	GCAGC	5 cut(s)	1238, 1357, 1870, 2092, 2437
BstV2I	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2192
BstX2I	RGATCY	7 cut(s)	388, 526, 641, 2099, 2162, 2179, 2297
BstYI	RGATCY	7 cut(s)	388, 526, 641, 2099, 2162, 2179, 2297
BsuRI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BtgZI	GCGATG	1 cut(s)	1143
BtrI	CACGTC	2 cut(s)	980, 2075
BtsCI	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2639
BtsI	GCAGTG	3 cut(s)	322, 477, 1668
BtsIMutI	CAGTG	9 cut(s)	322, 430, 477, 622, 1641, 1668, 1851, 1992, 2561
BveI	ACCTGC	1 cut(s)	2449
Cac8I	GCNNGC	7 cut(s)	84, 973, 1185, 1249, 1344, 2263, 2448
CaiI	CAGNNNCTG	3 cut(s)	254, 731, 1232
CciI	TCATGA	1 cut(s)	277
Cfr10I	RCCGGY	3 cut(s)	89, 1119, 2491
Cfr13I	GGNCC	4 cut(s)	943, 1418, 1665, 2353
CseI	GACGC	2 cut(s)	647, 1452
Csp6I	GTAC	2 cut(s)	110, 2522
CspAI	ACCGGT	2 cut(s)	89, 1119
CspCI	CAANNNNNGTGG	4 cut(s)	292, 327, 2076, 2111
CviAII	CATG	9 cut(s)	124, 278, 701, 1062, 1307, 1549, 1903, 2333, 2350
CviQI	GTAC	2 cut(s)	110, 2522
DraI	TTTAAA	1 cut(s)	1386
DraIII	CACNNNGTG	3 cut(s)	677, 2064, 2438
DriI	GACNNNNNGTC	1 cut(s)	618
EaeI	YGGCCR	4 cut(s)	136, 420, 1633, 2481
Eam1104I	CTCTTC	2 cut(s)	1145, 2348
Eam1105I	GACNNNNNGTC	1 cut(s)	618
EarI	CTCTTC	2 cut(s)	1145, 2348
Eco130I	CCWWGG	3 cut(s)	716, 772, 1871
Eco24I	GRGCYC	2 cut(s)	1040, 1522
Eco31I	GGTCTC	2 cut(s)	405, 1264
Eco32I	GATATC	1 cut(s)	1980
Eco47I	GGWCC	2 cut(s)	943, 1418
Eco72I	CACGTG	1 cut(s)	2531
Eco91I	GGTNACC	2 cut(s)	669, 1115
EcoNI	CCTNNNNNAGG	1 cut(s)	652
EcoO65I	GGTNACC	2 cut(s)	669, 1115
EcoRII	CCWGG	5 cut(s)	289, 504, 1031, 2392, 2483
EcoRV	GATATC	1 cut(s)	1980
EcoT14I	CCWWGG	3 cut(s)	716, 772, 1871
EcoT22I	ATGCAT	1 cut(s)	2338
EcoT38I	GRGCYC	2 cut(s)	1040, 1522
ErhI	CCWWGG	3 cut(s)	716, 772, 1871
Esp3I	CGTCTC	1 cut(s)	2065
FaeI	CATG	9 cut(s)	127, 281, 704, 1065, 1310, 1552, 1906, 2336, 2353
FalI	AAGNNNNNCTT	2 cut(s)	989, 1021
FaqI	GGGAC	2 cut(s)	1510, 2560
FatI	CATG	9 cut(s)	123, 277, 700, 1061, 1306, 1548, 1902, 2332, 2349
FbaI	TGATCA	3 cut(s)	190, 259, 1558
Fnu4HI	GCNGC	5 cut(s)	1227, 1371, 1884, 2106, 2451
FokI	GGATG	5 cut(s)	678, 1085, 1280, 1583, 2626
FriOI	GRGCYC	2 cut(s)	1040, 1522
Fsp4HI	GCNGC	5 cut(s)	1227, 1371, 1884, 2106, 2451
FspBI	CTAG	4 cut(s)	501, 773, 1139, 1791
GluI	GCNGC	5 cut(s)	1227, 1371, 1884, 2106, 2451
GsaI	CCCAGC	2 cut(s)	1208, 1498
GsuI	CTGGAG	4 cut(s)	387, 616, 1054, 1455
HaeIII	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
HapII	CCGG	7 cut(s)	69, 90, 139, 946, 1120, 1813, 2492
HgaI	GACGC	2 cut(s)	647, 1452
Hin1I	GRCGYC	2 cut(s)	658, 1444
Hin1II	CATG	9 cut(s)	127, 281, 704, 1065, 1310, 1552, 1906, 2336, 2353
HincII	GTYRAC	1 cut(s)	208
HindII	GTYRAC	1 cut(s)	208
HindIII	AAGCTT	1 cut(s)	1326
HinfI	GANTC	9 cut(s)	158, 268, 410, 598, 705, 937, 1336, 2210, 2326
HpaI	GTTAAC	1 cut(s)	208
HpaII	CCGG	7 cut(s)	69, 90, 139, 946, 1120, 1813, 2492
HphI	GGTGA	7 cut(s)	663, 1109, 1114, 1178, 2298, 2453, 2518
Hpy166II	GTNNAC	5 cut(s)	208, 834, 873, 2014, 2056
Hpy188I	TCNGA	7 cut(s)	168, 256, 559, 1333, 1558, 1972, 2584
Hpy8I	GTNNAC	5 cut(s)	208, 834, 873, 2014, 2056
HpyAV	CCTTC	7 cut(s)	29, 842, 1500, 1561, 1702, 1762, 2589
HpyCH4III	ACNGT	9 cut(s)	212, 233, 571, 620, 958, 1212, 1846, 1996, 2198
HpyCH4IV	ACGT	4 cut(s)	979, 1746, 2074, 2530
HpyF10VI	GCNNNNNNNGC	7 cut(s)	73, 1193, 1232, 1325, 1495, 2258, 2552
HpySE526I	ACGT	4 cut(s)	979, 1746, 2074, 2530
Hsp92I	GRCGYC	2 cut(s)	658, 1444
Hsp92II	CATG	9 cut(s)	127, 281, 704, 1065, 1310, 1552, 1906, 2336, 2353
Ksp22I	TGATCA	3 cut(s)	190, 259, 1558
KspAI	GTTAAC	1 cut(s)	208
LmnI	GCTCC	4 cut(s)	790, 1035, 1378, 2499
Lsp1109I	GCAGC	5 cut(s)	1238, 1357, 1870, 2092, 2437
LweI	GCATC	2 cut(s)	1120, 2248
MaeI	CTAG	4 cut(s)	501, 773, 1139, 1791
MaeII	ACGT	4 cut(s)	979, 1746, 2074, 2530
MfeI	CAATTG	1 cut(s)	930
MflI	RGATCY	7 cut(s)	388, 526, 641, 2099, 2162, 2179, 2297
MhlI	GDGCHC	3 cut(s)	1040, 1522, 2536
MlsI	TGGCCA	2 cut(s)	422, 2483
MluCI	AATT	8 cut(s)	273, 340, 394, 573, 930, 1851, 2401, 2419
MluNI	TGGCCA	2 cut(s)	422, 2483
MlyI	GAGTC	7 cut(s)	152, 404, 607, 946, 1330, 2204, 2320
Mox20I	TGGCCA	2 cut(s)	422, 2483
Mph1103I	ATGCAT	1 cut(s)	2338
MscI	TGGCCA	2 cut(s)	422, 2483
MseI	TTAA	9 cut(s)	27, 207, 393, 510, 807, 1124, 1385, 2205, 2423
MslI	CAYNNNNRTG	1 cut(s)	1745
Msp20I	TGGCCA	2 cut(s)	422, 2483
MspI	CCGG	7 cut(s)	69, 90, 139, 946, 1120, 1813, 2492
MspR9I	CCNGG	8 cut(s)	69, 140, 291, 506, 1033, 1814, 2394, 2485
MunI	CAATTG	1 cut(s)	930
Mva1269I	GAATGC	2 cut(s)	721, 2336
MvaI	CCWGG	5 cut(s)	291, 506, 1033, 2394, 2485
MwoI	GCNNNNNNNGC	7 cut(s)	73, 1193, 1232, 1325, 1495, 2258, 2552
NciI	CCSGG	3 cut(s)	69, 140, 1814
NlaIII	CATG	9 cut(s)	127, 281, 704, 1065, 1310, 1552, 1906, 2336, 2353
NlaIV	GGNNCC	7 cut(s)	301, 528, 1037, 1488, 1666, 1713, 2164
NmuCI	GTSAC	8 cut(s)	494, 620, 669, 680, 1115, 1990, 2149, 2506
NsiI	ATGCAT	1 cut(s)	2338
NspI	RCATGY	1 cut(s)	1065
NspV	TTCGAA	1 cut(s)	887
PagI	TCATGA	1 cut(s)	277
PctI	GAATGC	2 cut(s)	721, 2336
PfeI	GAWTC	2 cut(s)	268, 705
PfoI	TCCNGGA	1 cut(s)	1031
PinAI	ACCGGT	2 cut(s)	89, 1119
PkrI	GCNGC	5 cut(s)	1228, 1372, 1885, 2107, 2452
PleI	GAGTC	7 cut(s)	152, 404, 606, 945, 1330, 2204, 2320
PmaCI	CACGTG	1 cut(s)	2531
PmlI	CACGTG	1 cut(s)	2531
PpsI	GAGTC	7 cut(s)	152, 404, 606, 945, 1330, 2204, 2320
Ppu21I	YACGTR	1 cut(s)	2531
PshAI	GACNNNNGTC	1 cut(s)	597
PsiI	TTATAA	2 cut(s)	353, 918
Psp6I	CCWGG	5 cut(s)	289, 504, 1031, 2392, 2483
PspCI	CACGTG	1 cut(s)	2531
PspEI	GGTNACC	2 cut(s)	669, 1115
PspFI	CCCAGC	2 cut(s)	1204, 1494
PspGI	CCWGG	5 cut(s)	289, 504, 1031, 2392, 2483
PspN4I	GGNNCC	7 cut(s)	301, 528, 1037, 1488, 1666, 1713, 2164
PspPI	GGNCC	4 cut(s)	943, 1418, 1665, 2353
PstI	CTGCAG	1 cut(s)	45
PstNI	CAGNNNCTG	3 cut(s)	254, 731, 1232
PsuI	RGATCY	7 cut(s)	388, 526, 641, 2099, 2162, 2179, 2297
RsaI	GTAC	2 cut(s)	111, 2523
RsaNI	GTAC	2 cut(s)	110, 2522
RseI	CAYNNNNRTG	1 cut(s)	1745
SaqAI	TTAA	9 cut(s)	27, 207, 393, 510, 807, 1124, 1385, 2205, 2423
SatI	GCNGC	5 cut(s)	1227, 1371, 1884, 2106, 2451
Sau96I	GGNCC	4 cut(s)	943, 1418, 1665, 2353
SchI	GAGTC	7 cut(s)	152, 404, 607, 946, 1330, 2204, 2320
ScrFI	CCNGG	8 cut(s)	69, 140, 291, 506, 1033, 1814, 2394, 2485
SduI	GDGCHC	3 cut(s)	1040, 1522, 2536
SfaNI	GCATC	2 cut(s)	1120, 2248
SfcI	CTRYAG	1 cut(s)	41
SfuI	TTCGAA	1 cut(s)	887
SgrAI	CRCCGGYG	1 cut(s)	89
SinI	GGWCC	2 cut(s)	943, 1418
SmiMI	CAYNNNNRTG	1 cut(s)	1745
SmlI	CTYRAG	2 cut(s)	737, 1289
SmoI	CTYRAG	2 cut(s)	737, 1289
Sse9I	AATT	8 cut(s)	273, 340, 394, 573, 930, 1851, 2401, 2419
SsiI	CCGC	1 cut(s)	8
SspI	AATATT	2 cut(s)	2112, 2477
SspMI	CTAG	4 cut(s)	501, 773, 1139, 1791
StyD4I	CCNGG	8 cut(s)	67, 138, 289, 504, 1031, 1812, 2392, 2483
StyI	CCWWGG	3 cut(s)	716, 772, 1871
TaaI	ACNGT	9 cut(s)	212, 233, 571, 620, 958, 1212, 1846, 1996, 2198
TaiI	ACGT	4 cut(s)	982, 1749, 2077, 2533
TaqI	TCGA	6 cut(s)	413, 887, 1397, 1821, 1867, 2346
TasI	AATT	8 cut(s)	273, 340, 394, 573, 930, 1851, 2401, 2419
TatI	WGTACW	1 cut(s)	2521
TfiI	GAWTC	2 cut(s)	268, 705
Tru1I	TTAA	9 cut(s)	27, 207, 393, 510, 807, 1124, 1385, 2205, 2423
Tru9I	TTAA	9 cut(s)	27, 207, 393, 510, 807, 1124, 1385, 2205, 2423
TscAI	CASTG	9 cut(s)	322, 430, 484, 629, 1648, 1675, 1851, 1999, 2568
TseFI	GTSAC	8 cut(s)	494, 620, 669, 680, 1115, 1990, 2149, 2506
TseI	GCWGC	5 cut(s)	1226, 1370, 1883, 2105, 2450
Tsp45I	GTSAC	8 cut(s)	494, 620, 669, 680, 1115, 1990, 2149, 2506
TspDTI	ATGAA	7 cut(s)	140, 266, 532, 676, 1367, 1749, 2628
TspGWI	ACGGA	3 cut(s)	176, 2206, 2532
TspRI	CASTG	9 cut(s)	322, 430, 484, 629, 1648, 1675, 1851, 1999, 2568
VpaK11BI	GGWCC	2 cut(s)	943, 1418
XagI	CCTNNNNNAGG	1 cut(s)	652
XapI	RAATTY	2 cut(s)	2401, 2419
XceI	RCATGY	1 cut(s)	1065
XmaJI	CCTAGG	1 cut(s)	772
XspI	CTAG	4 cut(s)	501, 773, 1139, 1791
Zsp2I	ATGCAT	1 cut(s)	2338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.